Effect of one-year cover crop and arbuscular mycorrhiza inocululation in the microbial soil community of a vineyard

The advance in maturation of wine grapes is an important climate change risk related effect that could affect warm regions like Portuguese Douro Wine Region. Indeed, the climate analysis over the past years registered a decrease in the precipitation, significant higher average temperatures, and a more frequent occurrence of extreme weather events, including heat waves. In these conditions the length from anthesis until maturation is shortened and the uncoupling of technical and phenolic maturity results in berries with higher sugar concentration (and lower acidity), but lower anthocyanins, tannins, and total phenolic concentration, which produce unbalanced wines.
In this work, an innovative strategy of crop forcing, based on forcing vine regrowth after a second pruning of green shoots, was tested, aimed at delaying ripening until the temperature becomes lower and, therefore, preventing acidity loss and increasing anthocyanin-to-sugar ratio. The experiments were conducted in 2019 and 2020 in a commercial vineyard of ‘Touriga Nacional’ located in the Douro Region. Crop forcing was conducted 15 (CF1) to 30 (CF2) days after fruit set. Vines pruned with conventional methods were used as control (CF0). Results confirmed that fruit ripening was shifted from the hot season (August/September), until a cooler period (October through early-November). At harvest, grapevine berries from CF1 and CF2 presented lower pH and higher acidity, than control, with no significant differences in colour intensity and phenolic levels composition. Sugar content was lower in CF2-treated vines in both seasons. However, in CF-treated vines the number and size of clusters were significantly lower (up to 88% reduction) than in control plants. A metabolomics analysis of mature berries from CF-treated vines and control is underway. Crop forcing was indeed effective in producing a more balance berry composition but severely reduced grapevine yield,

The Grenache Noir grape variety, due to its originality and its representativeness, contributes very directly to the quality and typicality of the wines of the Rhône Valley. It is generally appreciated for its varied aromatic palette and for the roundness and suppleness it gives to wines. Since 1995, the Rhodanien Institute has set up a network of reference plots representative of the different types of terroir present in the southern zone of the Côtes du Rhône Appellation (TRUC, 1997; VAUDOUR et al, 1996 ) . Publications on the aromatic composition of grapes and wines are very abundant, but only a few articles have appeared on the Grenache grape variety PAUMES et al., 1986).

he intensification of t vineyard practices led to an impoverishment of the biological diversity. In vineyard management, the reflection to reduce pesticides uses concerns mainly the soil management of the vineyard, and often focuses on flora management in the inter-row.

In field conditions, grapevine roots are colonized by arbuscular mycorrhizal fungi (AMF). Little is known about the species composition of AMF communities associated to grapevine.

Nitrogen (N) nutrition of the vineyard plays a crucial role in the composition of must and wine, impacting fermentation, as well as the aroma and taste of the final product. N-deficient grape juice can result in increased astringency and bitterness, and a decrease in pleasant aromas in the wine.