Effect of one-year cover crop and arbuscular mycorrhiza inocululation in the microbial soil community of a vineyard

This study investigates the effect of wood chips addition during the alcoholic fermentation on the phenolic
composition of the produced wines. A series of wood chips, originating from American, French, Slavonia
oak and Acacia were added at the beginning of wine alcoholic fermentation. Besides, a mixture consisting
of 50% French and 50% Americal oak chips were added during the experimentation. The wine samples
were analyzed one month after the end of malolactic fermentation, examining various chemical
parameters such as total anthocyanins, total phenolic content, tannins combined with protein (BSA) and
ellagitannin content.

Ethyl acetate (EtOAc) is the main ester present in all wines, generally produced by yeasts during alcoholic fermentation and sometimes by bacteria during barrel ageing. Its odor is characterized by solvent notes, which give wines their acescent note [1].

Polyphenols are important compounds involved in many chemical and sensory wine features. In winemaking, adding oenological tannins claims to have positive impacts on wine stability, protection from oxidation and aroma persistence. Polyphenols are antioxidant compounds by either scavenging reactive oxygen and nitrogen species or chelating Fe2+ ions (1). However, as tannins oxidation leads to the formation of highly reactive species (i.e. ortho-quinones), it is still unclear if they have an effective role toward oxidation of wine aromas (2). In this work, we aim at studying the effect of two commercial tannins (proanthocyanidins, ellagitannins) on red wine flavour (mainly aroma) before and after air exposition.

Many works have been carried out to identify the key aroma volatile compounds of oak wood (e.g., whisky-lactone, furfural, maltol, eugenol, guaiacol, vanillin) using conventional gas chromatography coupled with olfactometry and mass spectrometry (GC-O-MS). Inspired by recent untargeted approaches in the field of food “omics”, this work aims to extend our knowledge on the impact of cooperage process on the volatile composition of oak wood using two-dimensional comprehensive gas chromatography coupled with time of flight mass spectrometry (GCxGC-ToFMS).

Grapevine leaf removal (LR) in the cluster area is typically done between fruit set and cluster closure to create an unfavorable microclimate for fungal diseases, such as Botrytis cinerea and powdery mildew. Grape growers are now turning their attention to pre-flowering LR, which has additional benefits under certain conditions. When applied before flowering, LR strongly affects fruit set and thus the number of berries per cluster. It is therefore a good yield control tool, replacing time-consuming manual cluster thinning (Poni et al. 2006). It also improves berry structure, that is, skin thickness, skin-to-pulp ratio, and berry composition (total soluble solids, titratable acidity, and polyphenols) (Palliotti et al. 2012; Komm and Moyer 2015). By exacerbating competition for assimilates between reproductive and vegetative organs, pre-flowering LR also poses some risks. Excessive yield loss at the same year’s harvest due to a too low fruit set rate is the main concern: intensive pre-flowering LR (100% of the cluster area) can induce up to 50% yield loss in potted vines (Poni et al. 2005). Other parameters, such as cool climatic conditions during flowering, also affect fruit set rate and make it difficult to predict potential yield at harvest. Repeated and overly intensive preflowering LR can have repercussions over time and induce a decline in bud fruiting and plant vigor (Risco et al. 2014).