The use of rootstock as a lever in the face of climate change and dieback of vineyard

Epidermal Patterning Factors are a class of cysteine rich peptides known to be involved in many developmental processes. The role of EPF1, EPF2 and EPFL9 in controlling leaf stomata formation has been well described in model plants and cereals, and recently also in grapevine, while little is known about their activity in other organs. The aim of our study is to investigate whether VviEPFL9-2 can have a specific biological function in grapevine roots, where it resulted to be expressed. As grapevine is cultivated in the form of a grafted plant, we focused our study on the commonly used rootstock Kober 5BB (Vitis berlandieri x Vitis riparia). VviEPFL9-2 was edited in Kober 5BB plants using Agrobacterium tumefaciens transformation of embryogenic calli and the CRISPR/Cas9 technology. The phenotypic evaluation in greenhouse indicated that, as expected, the leaves of knock-out (KO) plants have a significant lower stomatal density compared to WT, associated with a lower stomatal conductance.

Grape composition is of high interest for producing quality wines. For that, grape analyses are necessary, and they still require sample preparation, whether with classical analyses or with NIR analyses.

Le “terroir” est dans tous les discours, les articles, les étiquettes et les publicités. Le voca­ble est en situation d’utilisation euphorique. Indiscutablement l’emploi historique est agri­cole, puis viticole, mais il n’est jamais juridique.

Mandilaria, a red grape variety indigenous to the Aegean islands, is well known for its robust tannins and pronounced astringency, which can challenge the palatability and marketability of its wines. The aim of this study was the reduction of astringency in wines made exclusively from mandilaria grapes through dehydrations practices and targeted winery applications.

Grapevine is subject to multiple stresses, either biotic or abiotic, frequently in combination. These stresses may negatively impact the health status of plants and reduce yields. For biotic stress, grapevine is affected by numerous pest and diseases such as downy and powdery mildews, grey mold, black rot, grapevine fanleaf virus and trunk diseases (namely GTDs). The interaction between grapevine and pathogens is relatively complex and linked to various pathogenicity factors including cell-wall-degrading enzymes (especially CAZymes) and phytotoxic secondary metabolites, growth regulators, effectors proteins, and fungal viruses.