IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Origin of unpleasant smelling sulphur compounds during wine fermentation

Origin of unpleasant smelling sulphur compounds during wine fermentation

Abstract

The wine sector is undergoing considerable transformation, particularly as a result of climate change and increasing consumer expectations for quality products, in a globalised and increasingly competitive market. Therefore, the control of the sensory quality of wines is a major challenge that the actors of the sector have to overcome, promoting the formation of compounds with positive contribution while limiting the production of off-flavours. This requires a thorough understanding of the underlying mechanisms and the factors that can modulate these productions. Significant research efforts have been made to produce this information for positive compounds, but data on negative molecules remain very sparse.

Volatile sulphur compounds (VSCs) are considered as responsible for the reduction defect of wines, a regular issue for winemakers. These molecules, belonging mainly to the chemical families of thiols, thioesters, sulphides and disulphides, are formed during the fermentation and aging of wines. Their production involves the metabolism of yeast but also chemical reactions, as well responsible for many interconversions between these compounds. The main objective of our project was to provide a comprehensive view of the formation of VSCs during fermentation and its regulation by environmental factors.

To elucidate the metabolic and chemical molecular basis of these production and the better understand the regulatory mechanisms, two complementary lines of research were developed. A chemical approach spiking ongoing fermentation with sulphur compounds and incubating samples with and without cells was carried out. This enabled us to discriminate between enzymatic and chemical reactions within the VSCs formation network and to unravel the interconnections between compounds. Furthermore, the dynamics of formation of VSCs was monitored both in liquid samples and in the headspace of fermenters, directly connected to a gas chromatography device to detect extremely volatile compounds, as sulphide and methanethiol. The sequence of VSCs production, including the transient formation of some molecules, was therefore established. Our data clearly showed the involvement of methionine and cysteine as precursors for the biological formation of VSCs, as well as the key role of methanethiol as metabolic hub. In addition, our results highlighted the existence of chemical and biological interconversions between thiols, thioesters and disulphides that contribute to the VSC profile of wines. Overall, extending our knowledge on the VSCs formation and origin during wine fermentation, this study provided clues for the design of strategies to control the formation of these unpleasant smelling molecules during wine fermentation

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Carole Camarasa

¹INRA Montpellier France

Contact the author

Keywords

volatile sulphur compounds, metabolism, fermentation, ageing of wine

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

The relationship between wind exposure and viticultural performance of Vitis vinifera L. cv. Merlot in a coastal vineyard (South Africa)

The South Western Cape of South Africa is exposed to strong southerly and south easterly synoptic winds during the growth period of the grapevine. The development of sea breezes in the afternoon is also a phenomenon associated with the ripening period of grapes cultivated in this coastal area. Wind is one of the environmental variables having the greatest spatial variation but the implications of regular exposure to wind for the performance of the grapevine has not yet been determined for vineyards in the South Western Cape. This study was initiated to meet this need.
The study was conducted in a hedge-trellised vineyard of Vitis vinifera L. cv Merlot with north east – south west row direction. Thirty experimental sites, each consisting of 14 vines, were identified as being exposed to wind or sheltered based on hand-held anemometer readings during the 2001/2002 season. Four stationary anemometers were strategically positioned between the thirty sites. Stomatal conductance and leaf temperature were measured with a PP systems porometer. Vegetative and yield measurements were performed during the 2002/2003 season. The t-test of equal variance was used to determine significant differences in measured parameters between exposed and sheltered grapevines.
Stomatal conductance and leaf area were significantly reduced by exposure to wind. This was associated with a significant reduction in the leaf area of primary shoots, related to shorter shoots, but a significant augmentation of secondary shoot leaf number and area. The number of bunches per vine and yield were also reduced for exposed vines. The berry potassium content was significantly increased for exposed grapevines.
This demonstrates that exposure to wind can result in significant within-vineyard, and potentially between-vineyard, variability in grapevine physiology, vegetative growth, yield and berry composition, with implications for wine style and quality.

Aromatic profile evolution of corvina, corvinone and rondinella grapes during withering

AIM AND METHODS: Grape withering is one of the key steps in the production of the most renowned red wines of the Valpolicella area, namely Amarone and Recioto. This practice, which was already used since Roman times, entails important modifications in grape composition and in the chemical and sensorial characteristics of the corresponding wines, especially in terms of aromatic profile. The aim of this research is evaluating the aromatic evolution during grape withering of the three main varieties used in Valpolicella wines: Corvina, Corvinone and Rondinella.Samples of the three varieties were analyzed at harvest and at different stages of withering, namely10%, 20% and 30% of weight loss. Free and glycosidically bound compounds were extracted and analyzed using Gas Chromatography- Mass Spectrometry (GC-MS). RESULTS: For all the samples the data were normalized to eliminate the effect of concentration due to grape dehydration. Terpene content and evolution varied considerably in relationship to grape variety. Corvinone was richer in cyclic terpenes (including phellandrene, limonene, and cymene) and they decreased during withering.

Energy partitioning and functionality of photosystem II in water-stressed grapevines during heatwaves revealed by continuous measurements of chlorophyll fluorescence

The increased intensity and frequency of heatwaves, coupled with prolonged periods of drought, are a significant threat to viticulture worldwide. During these conditions the more exposed leaves can show visible symptoms of heat damage. We monitored the functionality of photosystem II (PSII) in the field to better understand the impact of heatwaves on canopy performance. A factorial experiment was established in summer 2023 using Shiraz grapevines in the Barossa valley of South Australia, involving water-stressed and well-watered vines.

A phylogenomic study reveals the major dissemination routes of ‘Tempranillo Tinto’ in the Iberian Peninsula

‘Tempranillo Tinto’ is a black-berried Iberian cultivar that originated from a hybridization between cvs. ‘Benedicto’ and ‘Albillo Mayor’ [1]. Today, it is the third most widely grown wine grape cultivar worldwide with more than 200,000 hectares of vineyards mostly distributed along the Iberian Peninsula, where it is also known as ‘Cencibel’, ‘Tinta de Toro’, ‘Tinta Roriz’, and ‘Aragonez’, among other synonyms. Here, we quantified the intra-varietal genomic diversity in this cultivar through the study of 35 clones or ancient vines from seven different Iberian wine-making regions. A comparative analysis after Illumina whole-genome sequencing revealed the presence of 1,120 clonal single nucleotide variants (SNVs).

Study of fungal and bacterial laccases for the reduction of ochratoxin A content in model wine

Ochratoxin A (OTA) is a mycotoxin produced by several filamentous fungi infecting grape bunches (Penicillium and Aspergillus spp.), this toxin pass to must when grapes are crushed and later it is found in wine. Following the evaluations of the toxicity of OTA, European Commission Regulations have been promulgated introducing upper limits for OTA concentrations in various commodities (cereals, cereal products, dried vine fruit, coffee, wine, grape juice, baby foods and dietary foods for special medical purposes).