IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Origin of unpleasant smelling sulphur compounds during wine fermentation

Origin of unpleasant smelling sulphur compounds during wine fermentation

Abstract

The wine sector is undergoing considerable transformation, particularly as a result of climate change and increasing consumer expectations for quality products, in a globalised and increasingly competitive market. Therefore, the control of the sensory quality of wines is a major challenge that the actors of the sector have to overcome, promoting the formation of compounds with positive contribution while limiting the production of off-flavours. This requires a thorough understanding of the underlying mechanisms and the factors that can modulate these productions. Significant research efforts have been made to produce this information for positive compounds, but data on negative molecules remain very sparse.

Volatile sulphur compounds (VSCs) are considered as responsible for the reduction defect of wines, a regular issue for winemakers. These molecules, belonging mainly to the chemical families of thiols, thioesters, sulphides and disulphides, are formed during the fermentation and aging of wines. Their production involves the metabolism of yeast but also chemical reactions, as well responsible for many interconversions between these compounds. The main objective of our project was to provide a comprehensive view of the formation of VSCs during fermentation and its regulation by environmental factors.

To elucidate the metabolic and chemical molecular basis of these production and the better understand the regulatory mechanisms, two complementary lines of research were developed. A chemical approach spiking ongoing fermentation with sulphur compounds and incubating samples with and without cells was carried out. This enabled us to discriminate between enzymatic and chemical reactions within the VSCs formation network and to unravel the interconnections between compounds. Furthermore, the dynamics of formation of VSCs was monitored both in liquid samples and in the headspace of fermenters, directly connected to a gas chromatography device to detect extremely volatile compounds, as sulphide and methanethiol. The sequence of VSCs production, including the transient formation of some molecules, was therefore established. Our data clearly showed the involvement of methionine and cysteine as precursors for the biological formation of VSCs, as well as the key role of methanethiol as metabolic hub. In addition, our results highlighted the existence of chemical and biological interconversions between thiols, thioesters and disulphides that contribute to the VSC profile of wines. Overall, extending our knowledge on the VSCs formation and origin during wine fermentation, this study provided clues for the design of strategies to control the formation of these unpleasant smelling molecules during wine fermentation

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Carole Camarasa

¹INRA Montpellier France

Contact the author

Keywords

volatile sulphur compounds, metabolism, fermentation, ageing of wine

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Inhibition of Oenococcus oeni during alcoholic fermentation by a selected Lactiplantibacillus plantarum strain

The use of selected cultures of the species Lactiplantibacillus plantarum in Oenology has grown in prominence in recent years. While initial applications of this species centred very much around malolactic fermentation (MLF), there is strong evidence to show that certain strains can be harnessed for their bio-protective effects. Unwanted spontaneous MLF during alcoholic fermentation (AF), driven by rogue Oenococcus oeni, is a winemaking deviation that is very difficult to manage when it occurs. This work set out to determine the efficacy of one particular strain of Lactiplantibacillus plantarum(Viniflora® NoVA™ Protect), against this problem in Cabernet Sauvignon must. The work was carried out at commercial scale and in a winery environment and compared the bio-protective culture with the more traditional approach of reducing must pH by the addition of tartaric acid. The combination of both was also investigated. The concentration of both Oenococcus oeni and Lactiplantibacillus plantarum was determined using qPCR. The adventitious Oenococcus oeni showed the most growth during AF in the control wine, whereas in the wines treated with Lactiplantibacillus plantarum a bacteriostatic effect against this species was observed. This effect was comparable to the wines treated with tartaric acid. This has particular commercial relevance for controlling the flora in musts with high pH, or when the addition of tartaric acid is either not permitted or is prohibitive for other reasons.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Sustaining wine identity through intra-varietal diversification

With contemporary climate change, cultivated Vitis vinifera L. is at risk as climate is a critical component in defining ecologically fitted plant materiel. While winegrowers can draw on the rich diversity among grapevine varieties to limit expected impacts (Morales-Castilla et al., 2020), replacing a signature variety that has created a sense of local distinctiveness may lead to several challenges. In order to sustain wine identity in uncertain climate outcomes, the study of intra-varietal diversity is important to reflect the adaptive and evolutionary potential of current cultivated varieties. The aim of this ongoing study is to understand to what extent can intra-varietal diversity be a climate change adaptation solution. With a focus on early (Sauvignon blanc, Riesling, Grolleau, Pinot noir) to moderate late (Chenin, Petit Verdot, Cabernet franc) ripening varieties, data was collected for flowering and veraison for the various studied accessions (from conservatory plots) and clones. For these phenological growing stages, heat requirements were established using nearby weather stations (adapted from the GFV model, Parker et al., 2013) and model performances were verified. Climate change projections were then integrated to predict the future behaviour of the intra-varietal diversity. Study findings highlight the strong phenotypic diversity of studied varieties and the importance of diversification to enhance climate change resilience. While model performances may require improvements, this study is the first step towards quantifying heat requirements of different clones and how they can provide adaptation solutions for winegrowers to sustain local wine identity in a global changing climate. As genetic diversity is an ongoing process through point mutations and epigenetic adaptations, perspective work is to explore clonal data from a wide variety of geographic locations.