IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Combined high-resolution chromatography techniques and sensory analysis as a support decision system tool for the oenologist

Combined high-resolution chromatography techniques and sensory analysis as a support decision system tool for the oenologist

Abstract

One of the main challenges in the wine industry is to understand how different wine processing techniques and practices can influence the overall quality of the final product. Winemakers base the decision process mostly on their personal experience, which is often influenced by emotional aspects not always scientifically supported. Other issues come from the terroir and climate change, which are affecting the quality and production techniques, both in vineyards and wineries. In addition, it is important to consider that wine culture in the different production areas is also extremely variegated, even within the same country. Relying on analytical methods is a necessary step taken in many parts of the winemaking process, starting from the determination of the optimal time for the harvest. Monitoring the fermentation is usually performed by controlling the density or the residual sugars: secondary metabolites are usually not determined. This means that in some cases the fermentation can get stuck without really knowing the reason.

This research project aims to create a predictive multivariate statistical tool in order to support the winemaker during the workflow in the winery. So, the oenologist can obtain the desired style of wine by extracting information from correlating basic oenological parameters with high resolution and sensory analysis.

Pinot Noir cultivar is a very important variety for South Tyrol representing 9.1% of the local vineyard (source: vinialtoadige.com). The experimental scheme shown in figure 1 was developed in collaboration with a South Tyrolean winery. The study plan was aimed at ensuring control over the winemaking protocols while still working at the winery production scale (90 hL per experiment).

The experimental plan included four vineyards. Besides, for one of these vineyards, the plan included the study of a viticultural technique (treatment of the canopy with chitosan prior to harvest), and two different oenological treatments: pre fermentative 4-days cryo-maceration and 7-days grape freezing. The samples were analyzed by HS-SPME-GCxGC-ToF/MS for volatile compounds, HPLC-DAD-FLD for phenolic compounds with off-line HPLC-MS/MS to identify the components, and sensory analysis by quantitative descriptive analysis (QDA®) (Poggesi, et al., 2021). The study was repeated in two different vintages (2019 and 2020) with three replicates.

As a result, multivariate statistic models showed good separations between vineyards, frozen grapes, and the cryo-macerated treatment, and separation between chitosan treatment and the control treatment. Furthermore, the time evolution of the main chemical markers was evaluated. Finally, the results obtained on the 2019 vintage were supported by the 2020 ones

References

Alto Adige Wine – Exquisite Wines from Northern Italy (altoadigewines.com)
Poggesi, S., de Matos, A. D., Longo, E., Chiotti, D., Pedri, U., Eisenstecken, D., Robatscher, P., & Boselli, E. (2021). Chemosensory profile of South Tyrolean pinot blanc wines: A multivariate regression approach. Molecules, 26(20), 1–18. https://doi.org/10.3390/molecules26206245

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Poggesi Simone¹, Darnal¹, Merkyte¹, Longo¹, Montali²and Boselli ¹

¹Faculty of Science and Technology, Free University of Bozen-Bolzano
²Faculty of Computer Science, Free University of Bozen-Bolzano

Contact the author

Keywords

Pinot Noir, bidimensional gas chromatography, non-volatile phenols, support decision tool, sensory analysis

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Diagnosis of soil quality and evaluation of the impact of viticultural practices on soil biodiversity in a vineyard in southwestern France

Viticulture is facing two major changes – climate change and agroecological transition. In both cases, soil quality is seen as a lever to move towards a more sustainable viticulture. However, soil biological quality is little considered in the implementation of viticultural practices. Gascogn’Innov (2017-2022) is an Operational Group funded by the European Innovation Partnership for Agriculture. As such, it brings together winegrowers from the south-west of France, scientists, advisors and technicians, around a project focused on viticultural soil biological functioning and the design of technical routes more respectful toward soil heritage. To achieve this, the project aims to acquire references on the impact of viticultural practices on soil biology from a dynamic way, and to test a methodology to integrate information provided by the soil bioindicators to manage farming systems. A set of indicators of soil biological quality are evaluated in the project: microorganisms (bacteria and fungi abundance and diversity), fauna (abundance and diversity of nematodes and earthworms), physico-chemical characteristics, soil structure assessment and degradation rate of organic matter. Based on a network of 13 plots that have been subject to an initial diagnosis in 2017, several agronomical practices to restore soil fertility are experimented to redesign the cropping system (for instance plant cover, organic matter inputs, reduction of herbicides, mineral fertilizers). System redesign was made in collaboration by winegrowers and an interdisciplinary group of experts (agronomists, biologists). Several indicators are measured on vine and soil at each vintage to assess vine health and productivity. At the end of the project (2021), a final diagnosis was carried out. Gascogn’Innov allowed to create a regional database on the quality of wine-growing soils, which permitted to evaluate the effect of practices according to soil types. Especially, decreasing the intensity of tillage and increasing the duration and diversity of grass coverage tends to increase the abundance of all the organisms studied. This project confirmed the value of soil biological quality indicators to drive the sustainability of practices, but also highlighted the key-role of expertise, in both agronomy and soil biology, to help winegrowers understand and appropriate their soil quality diagnoses.

Vineyards and clay minerals: multi-technique analytical approach and correlations with soil properties

Purpose of this research is to quantitatively assess the mineral component of vineyard soils, with particular attention to the mineralogical analysis of clays, which represent an element of high importance in the vineyard culture as well as in general agriculture. An X-ray diffraction (XRD) / thermogravimetric (TG) multi-technique analytical approach was developed, tested on soil samples taken from vineyards around the world. This codified analytical procedure was necessary to obtain precise qualitative and quantitative mineralogical data, globally comparable to distinguish the geopedological identity of the vineyards. Soil samples from vineyards of various locations were analysed, in very different geological conditions. The bulk-rock quantitative phase analysis (QPA) was obtained by the Rietveld method while the detailed composition of the clay-sized fraction was determined by modelling of the oriented X-ray diffraction patterns. The research provided a precise classification of the mineral component of soils, distinguishing the mineral phases of the clays and the so-called mixed-layer clay minerals. We found that the content in mixed layers can be directly correlated with the water retention and the cation exchange capacity ​​of the soil, while the presence of other clayey minerals and phyllosilicates in this research did not affect this CEC parameter, which codes the fertility level of the soils. The study demonstrates that terroir, in particular soils formed in complex or very different geological conditions, can only be effectively interpreted by properly analysing its mineral phases, in particular the mixed-layer clay component. These are characteristic abiotic ecological indicators, which may have specific eco-physiological influences on the plant.

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.

Revealing the Barossa zone sub-divisions through sensory and chemical analysis of Shiraz wine

The Barossa zone is arguably one of the most well-recognised wine producing regions in Australia and internationally; known mainly for the production of its distinct Shiraz wines. However, within the broad Barossa geographical delimitation, a variation in terroir can be perceived and is expressed as sensorial and chemical profile differences between wines. This study aimed to explore the sub-division classification across the Barossa region using chemical and sensory measurements. Shiraz grapes from 4 different vintages and different vineyards across the Barossa (2018, n = 69; 2019, n = 72; 2020, n = 79; 2021, n = 64) were harvested and made using a standardised small lot winemaking procedure. The analysis involved a sensory descriptive analysis with a highly trained panel and chemical measurement including basic chemistry (e.g. pH, TA, alcohol content, total SO2), phenolic composition, volatile compounds, metals, proline, and polysaccharides. The datasets were combined and analysed through an unsupervised, clustering analysis. Firstly, each vintage was considered separately to investigate any vintage to vintage variation. The datasets were then combined and analysed as a whole. The number of sub-divisions based on the measurements were identified and characterised with their sensory and chemical profile and some consistencies were seen between the vintages. Preliminary analysis of the sensory results showed that in most vintages, two major groups could be identified characterised with one group showing a fruit-forward profile and another displaying savoury and cooked vegetables characters. The exploration of distinct profiles arising from the Barossa wine producing region will provide producers with valuable information about the regional potential of their wine assisting with tools to increase their target market and reputation. This study will also provide a robust and comprehensive basis to determine the distinctive terroir characteristics which exist within the Barossa wine producing region.

Making sense of available information for climate change adaptation and building resilience into wine production systems across the world

Effects of climate change on viticulture systems and winemaking processes are being felt across the world. The IPCC 6thAssessment Report concluded widespread and rapid changes have occurred, the scale of recent changes being unprecedented over many centuries to many thousands of years. These changes will continue under all emission scenarios considered, including increases in frequency and intensity of hot extremes, heatwaves, heavy precipitation and droughts. Wine companies need tools and models allowing to peer into the future and identify the moment for intervention and measures for mitigation and/or avoidance. Previously, we presented conceptual guidelines for a 5-stage framework for defining adaptation strategies for wine businesses. That framework allows for direct comparison of different solutions to mitigate perceived climate change risks. Recent global climatic evolution and multiple reports of severe events since then (smoke taint, heatwave and droughts, frost, hail and floods, rising sea levels) imply urgency in providing effective tools to tackle the multiple perceived risks. A coordinated drive towards a higher level of resilience is therefore required. Recent publications such as the Australian Wine Future Climate Atlas and results from projects such as H2020 MED-GOLD inform on expected climate change impacts to the wine sector, foreseeing the climate to expect at regional and vineyard scale in coming decades. We present examples of practical application of the Climate Change Adaptation Framework (CCAF) to impacts affecting wine production in two wine regions: Barossa (Australia) and Douro (Portugal). We demonstrate feasibility of the framework for climate adaptation from available data and tools to estimate historical climate-induced profitability loss, to project it in the future and to identify critical moments when disruptions may occur if timely measures are not implemented. Finally, we discuss adaptation measures and respective timeframes for successful mitigation of disruptive risk while enhancing resilience of wine systems.