IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Combined high-resolution chromatography techniques and sensory analysis as a support decision system tool for the oenologist

Combined high-resolution chromatography techniques and sensory analysis as a support decision system tool for the oenologist

Abstract

One of the main challenges in the wine industry is to understand how different wine processing techniques and practices can influence the overall quality of the final product. Winemakers base the decision process mostly on their personal experience, which is often influenced by emotional aspects not always scientifically supported. Other issues come from the terroir and climate change, which are affecting the quality and production techniques, both in vineyards and wineries. In addition, it is important to consider that wine culture in the different production areas is also extremely variegated, even within the same country. Relying on analytical methods is a necessary step taken in many parts of the winemaking process, starting from the determination of the optimal time for the harvest. Monitoring the fermentation is usually performed by controlling the density or the residual sugars: secondary metabolites are usually not determined. This means that in some cases the fermentation can get stuck without really knowing the reason.

This research project aims to create a predictive multivariate statistical tool in order to support the winemaker during the workflow in the winery. So, the oenologist can obtain the desired style of wine by extracting information from correlating basic oenological parameters with high resolution and sensory analysis.

Pinot Noir cultivar is a very important variety for South Tyrol representing 9.1% of the local vineyard (source: vinialtoadige.com). The experimental scheme shown in figure 1 was developed in collaboration with a South Tyrolean winery. The study plan was aimed at ensuring control over the winemaking protocols while still working at the winery production scale (90 hL per experiment).

The experimental plan included four vineyards. Besides, for one of these vineyards, the plan included the study of a viticultural technique (treatment of the canopy with chitosan prior to harvest), and two different oenological treatments: pre fermentative 4-days cryo-maceration and 7-days grape freezing. The samples were analyzed by HS-SPME-GCxGC-ToF/MS for volatile compounds, HPLC-DAD-FLD for phenolic compounds with off-line HPLC-MS/MS to identify the components, and sensory analysis by quantitative descriptive analysis (QDA®) (Poggesi, et al., 2021). The study was repeated in two different vintages (2019 and 2020) with three replicates.

As a result, multivariate statistic models showed good separations between vineyards, frozen grapes, and the cryo-macerated treatment, and separation between chitosan treatment and the control treatment. Furthermore, the time evolution of the main chemical markers was evaluated. Finally, the results obtained on the 2019 vintage were supported by the 2020 ones

References

Alto Adige Wine – Exquisite Wines from Northern Italy (altoadigewines.com)
Poggesi, S., de Matos, A. D., Longo, E., Chiotti, D., Pedri, U., Eisenstecken, D., Robatscher, P., & Boselli, E. (2021). Chemosensory profile of South Tyrolean pinot blanc wines: A multivariate regression approach. Molecules, 26(20), 1–18. https://doi.org/10.3390/molecules26206245

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Poggesi Simone¹, Darnal¹, Merkyte¹, Longo¹, Montali²and Boselli ¹

¹Faculty of Science and Technology, Free University of Bozen-Bolzano
²Faculty of Computer Science, Free University of Bozen-Bolzano

Contact the author

Keywords

Pinot Noir, bidimensional gas chromatography, non-volatile phenols, support decision tool, sensory analysis

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Green berries on Gewürztraminer (Vitis vinifera L.) in South Tyrol (Italy)

The grape variety Gewürztraminer is known to be affected by two physiological disorders namely berry shrivel and bunch stem necrosis. During the season 2014 we noticed a new symptomatology type of ripening disorder on the variety. The new symptom showed not all berries fallowing the normal maturation stages, but single berries remaining at a soft but green stage till harvest. The broad distribution of these so called “green berries” symptoms in different production sites of our region, caused huge damage due to the difficulty of eliminating single berries per bunch before harvesting. Therefore, the Research Centre Laimburg began to investigate the reasons and origins of this new symptom. This work shows the results of first attempts to find causes for the symptom as well as the resulting approach to mitigate symptoms. Applications of magnesium leaf fertilizer showed first promising results against this putative disorder. To study the causal effect of the green berries 30 symptomatic vineyards in 2014 have been selected for a monitoring during the season 2016. To evaluate the foliar nutrient treatment two vineyards have been selected for application of magnesium sulfate and magnesium chloride. Leaf and berry nutrient analysis, as well as the main quality parameters during ripening have been performed. As soon as “green berries” symptoms appeared, incidence and severity have been evaluated. Most of the symptomatic vineyards of the 2016 monitoring showed light to clear magnesium deficit symptoms on their foliage. Only during the seasons 2020 and 2021 “green berries” symptoms could be found in the leaf fertilizer treatment vineyards. Both seasons showed a significant effect of the magnesium treatments to reduce the incidence and severity of the symptom. It seems that the appearance of the “green berries” symptom on Gewürztraminer is correlated to a disturbed uptake of magnesium of the vines.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.

δ13C : A still underused indicator in precision viticulture  

The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
The lack of private laboratories proposing the analysis, the cost of the technology, as well as the long analytical delays, have been detrimental to its development. Some laboratories tried to overcome the analytical difficulties of isotopic analysis by using fourier transformed infrared spectroscopy, as a fast and cheap alternative to the official OIV method (IRMS). These claimed FTIR models have never been published or peer reviewed and cannot be considered robust. In this work, thanks to the recent acquisition of IRMS technology, new modern and robust applications of δ13C for viticulture are proposed. This includes the use of the analysis to make parcel separations at harvesting, the possibility to increase the precision of hydric stress cartography and the potential cost reduction when compared with Scholander pressure bomb analysis.

Effect of fertigation strategies to adapt PGI Côtes de Gascogne production to hot vintage

The development of fertigation could be a possible solution to adapt PGI Côtes de Gascogne (south-western France) wine production to climate change. The goal would be to limit the negative effects of water stress on yield performance expectation (around 15 tons per hectare) and to make the use of fertilizers more efficient. This study aimed to compare the effects of three strategies of water and minerals supply on grapes and wines qualities. Two fertigation practices were compared to a rainfed control which is the current standard of the local grape growing production. The fertilizers (nitrogen and potassium) were (i) fully brought by irrigation pipe during the season, (ii) partially brought by irrigation pipe and partially on the soil or (iii) fully brought on the soil at the beginning of the season for the non-irrigated control (local standard). The trial was run on cv. Colombard trained on spur pruned with vertical shoot positioning system on a sandy-silty-clay soil over the 2020 vintage which was particularly hot for the region. Moderate to strong water deficit appeared during the growing period of the berries and held on after veraison. Irrigation strategies allowed for maintaining grapevine without water deficit and being significantly different from the control water status. Grapevine with fully or partial fertigation strategies produced 25% more yield mainly due to the increase of the bunch weight. Also, the fully fertigation showed the best ratio between yield and maturity and brought 30% less of fertilizers (both nitrogen and potassium) than the two other strategies. Finally, the analysis of aromatic compounds in Colombard wines, varietal thiols family, showed the same level of concentrations for the 3 treatments, confirming that the yield performance did not impact the aromatic potential in this trial.

Impact of yeast derivatives to increase the phenolic maturity and aroma intensity of wine

Using viticultural and enological techniques to increase aromatics in white wine is a prized yet challenging technique for commercial wine producers. Equally difficult are challenges encountered in hastening phenolic maturity and thereby increasing color intensity in red wines. The ability to alter organoleptic and visual properties of wines plays a decisive role in vintages in which grapes are not able to reach full maturity, which is seen increasingly more often as a result of climate change. A new, yeast-based product on the viticultural market may give the opportunity to increase sensory properties of finished wines. Manufacturer packaging claims these yeast derivatives intensify wine aromas of white grape varieties, as well as improve phenolic ripeness of red varieties, but the effects of this application have been little researched until now. The current study applied the yeast derivative, according to the manufacture’s instructions, to the leaves of both neutral and aromatic white wine varieties, as well as on structured red wine varieties. Chemical parameters and volatile aromatics were analyzed in grape musts and finished wines, and all wines were subjected to sensory analysis by a tasting panel. Collective results of all analyses showed that the application of the yeast derivative in the vineyard showed no effect across all varieties examined, and did not intensify white wine aromatics, nor improve phenolic ripeness and color intensity in red wine.