IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Infrared spectroscopy investigation of fresh grapevine organs for clustering and classification.

Infrared spectroscopy investigation of fresh grapevine organs for clustering and classification.

Abstract

The spectral information acquired from fresh whole grapevine organs have yet to be fully explored. Infrared spectroscopy provides the means to rapidly measure fresh plant material and providing extensive information on the physical and chemical structure of samples. This study aimed to explore the spectra of fresh grapevine shoots, leaves, and berries throughout the growing season for clustering and classification. Sampling was performed across two vintages (2019-2020; 2020-2021) from November to March. Five locations, seven cultivars, and 17 commercial vineyards were included. Collection of whole shoots, including leaves and grape bunches, were performed on a monthly basis. The fresh grapevine organs were analysed using three spectroscopy methods within 24-36 hours of sampling. Mid-infrared (MIR) and near-infrared (NIR), making use of a solid probe (NIR-SP) and a rotating sphere (NIR-RS), were investigated. The raw spectra were firstly investigated using principal component analysis (PCA) followed by a more novel chemometric approach, unsupervised
self-organising maps (SOM). PCA as well as unsupervised SOM showed the most considerable grouping based on organ type. Additionally, the unsupervised SOM showed separation trends based on phenological stage. Investigation of the datasets per organ with SOM showed separation based on the phenological stage for berries and shoots, as well as shoots clustering based on lignification. Supervised SOM were examined for classification based on the observed clustering per organ type, phenological stage, and lignification. The accurate prediction of organ at 90.3% was possible for the NIR-SP dataset for 2019-2021. Overlapping of various phenological stages were seen for the grape berry datasets, but prediction improved to 85.6% for the NIR-RS 2019-2021 dataset when certain phenological
stages were grouped together. Accurate predictions of lignified and unlignified shoots were also seen for the NIR-SP 2019-2021 and NIR-RS 2020-2021 datasets at 74.4% and 89.9% respectively. The possibility of using spectral variable selection to improve the supervised SOM predictions were explored and promising results obtained for certain datasets. Following variable selection with OPLS-DA and S-plots, the prediction of shoots and leaves improved by 14% for the NIR-RS 2020-2021 dataset. The prediction of lignified and unlignified shoots improved considerably to 92.3% for the NIR-SP 2019-2021 dataset and 95.9% for the NIR-RS 2020-2021 dataset. This study showed the extensive information available in infrared spectra of fresh grapevine organs and how the information could be used to achieve important clustering and classifications objectives

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Van Wyngaard Elizma¹, Blancquaert Erna¹, Nieuwoudt Hélène¹and Aleixandre-Tudo Jose Luis1,²

¹South African Grape and Wine Research Institute (SAGWRI), Department of Viticulture and Oenology, Stellenbosch University, Stellenbosch, South Africa
²Instituto de Ingeniería de Alimentos para el Desarrollo (IIAD), Departamento de Tecnologia de Alimentos, Universidad Politécnica de Valencia, Valencia, Spain

Contact the author

Keywords

Spectroscopy, grapevine organs, clustering, classification

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Under-vine management effects on grapevine production, soil properties and plant communities in South Australia

Under-vine (UV) management has traditionally consisted of synthetic herbicide use to limit competition between weeds and grapevines. With growing global interest towards non-synthetic chemical use, this study aimed to capture the effects of alternative UV management at two commercial Shiraz vineyards in South Australia, where the sole management variables were UV management since 2016. In adjacent treatment blocks, cultivation (CU) was compared to spontaneous vegetation (SV) in McLaren Vale (MV), and herbicide was compared to SV in Eden Valley (EV). Soil water infiltration rates were slower and grapevine stem water potential was lower in CU compared to SV in MV, with the latter having a plant community dominated by soursob (Oxalis pes-caprae) during winter; while in EV, there was little separation between the treatments. Yields were affected at both sites, with SV being higher in MV and HE being higher in EV. In MV, the only effect on grape must was a lower 13C:12C isotope ratio in CU, indicating greater grapevine water stress. In the grape must at EV, SV had higher total soluble solids, total phenolics, anthocyanins, and yeast available nitrogen; and lower pH and titratable acidity. Pruning weights were not affected by the treatments in MV, while they were higher in HE at EV. Assessments revealed that the differing soil types at the two sites were likely the main determinants of the opposing production outcomes associated with UV management. In the silty loam soil of MV, the higher yields in SV were likely due to more plant-available water, as a potential result of the continuous soil bio-pores formed by winter UV vegetation. Conversely, in the loamy sand soils of EV with a lower cation exchange capacity, the lower yields and pruning weights in SV suggest the UV vegetation competed significantly with the grapevines for available water and nutrients.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Effect of regulated deficit irrigation regime on amino acids content of Monastrell (Vitis vinifera L.) grapes

Irrigation is an important practice to influence vine quality, especially in Mediterranean regions, characterized by hot summers and severe droughts during the growing season. This study focused on deficit irrigation regime influence on amino acids composition of Monastrell grapevines under semiarid conditions (Albacete, Southeastern of Spain). In 2019, two treatments were applied: non-irrigation (NI) and regulated deficit irrigation (RDI), watered at 30% of the estimated crop evapotranspiration from fruit set to onset of veraison. Grape amino acids content was analyzed by HPLC. Berries from non-irrigated vines showed higher concentration of several amino acids, such as tryptophan (73%), arginine (70%), lysine (36%), isoleucine (27%), and leucine (21%), compared to RDI grapes. Arginine is, together with ammonium ion, the principal nitrogen source for yeasts during the alcoholic fermentation; while isoleucine, tryptophan, and leucine are precursors of fermentative volatile compounds, key compounds for wine quality. Moreover, NI treatment increased in a 14% the total amino acids content in grapes compared to RDI treatment. The reported effects might be because yield was 70% higher in RDI vines than in the NI ones and, therefore, the sink demand was increased in the irrigated vines. In addition, NI vines suffered more severe water stress and it is known that the amino acids synthesis and accumulation can be influenced by the plant response to stress. According to the results, the irrigation regime showed effect on amino acids concentration in Monastrell grapes under semiarid conditions. Grapes from non-irrigated vines showed a higher content of several amino acids relevant to the fermentative process and to the wine aroma compounds formation. It is demonstrated that the final content of nitrogen-related components in grapes is influenced by the irrigation regime. The convenience of the irrigation strategy to suggest will depend on the desired wine style and the target yield levels.

Phenological characterization of a wide range of Vitis Vinifera varieties

In order to study the impact of climate change on Bordeaux grape varieties and to assess the adaptation capacities of candidates to the grape varieties of this wine region to the new climatic conditions, an experimental block design composed of 52 grape varieties was set up in 2009 at the INRAE Bordeaux Aquitaine center. Among the many parameters studied, the three main phenological stages of the vine (budburst, flowering and veraison) have been closely monitored since 2012. Observations for each year, stage and variety were carried out on four independent replicates. Precocity indices have been calculated from the data obtained over the 2012-2021 period (Barbeau et al. 1998). This work allowed to group the phenological behaviour of the grapevine varieties, not only based on the timing of the subsequent developmental stages, but also on the overall precocity of the cycle and the total length of the cycle between budburst and veraison. Results regarding the variability observed among the different grape varieties for these phenological stages are presented as heat maps.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…