IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Application of grape pomace and stem extracts on Vitis vinifera L. cv. Monastrell: Increased stilbene content of grapes and wines

Application of grape pomace and stem extracts on Vitis vinifera L. cv. Monastrell: Increased stilbene content of grapes and wines

Abstract

Pomace and grape stems are the main solid organic waste from winery industries, resulting from the pressing and/or fermentation processes it is generated in large amounts in many parts of the world, and disposed of in open areas causing environmental and economic problems.1,2 Therefore, it is necessary to look for alternatives to revalue these bioproducts, making the winemaking process a more sustainable activity. Therefore, this work aims to determine whether grape pomace extracts can be used as elicitors to increase the biosynthesis of healthy compounds in wine grapes.The experiment was carried out in 2021. Two polyphenolic extracts were obtained: one from pomace and the other from grape stems. Subsequently, the extracts were sprayed on Vitis vinifera L. cv Monastrell at the beginning of veraison (1st application) and seven days later (2nd application). When grapes reached technological maturity, they were harvested and transported in boxes to the winery for physicochemical analysis and vinification. Wines were analyzed at the end of alcoholic fermentation.The results indicated a significant increase in the concentration of grape stilbenes, especially by treatment with pomace extracts. The following stilbenes were increased: T-piceid, piceatanol, C-piceid, T-resveratrol and viniferins. The wines produced also showed a higher concentration of stilbenes compared to the wines from control grapes; mainly T-resveratrol and viniferins were released into the wine.In this sense, stilbenes are of particular importance in plants as they are synthesized under biotic or abiotic stress, giving the plant greater resistance to fungal attack.3 On the other hand, resveratrol has been extensively studied for its importance in health, as it is attributed with antioxidant, anticarcinogenic, neuroprotective and cardioprotective properties.4 Therefore, these results show that the use of pomace and grape stem extracts are a very interesting alternative since they would allow: revaluing the winery’s bioproducts, reducing the use of synthetic pesticides and increasing the functional value of grapes and wines.

References

1 Beres C, Costa GNS, Cabezudo I, Silva-James NK da, Teles ASC, Cruz APG, Mellinger-Silva C, Tonon R V, Cabral LMC, and Freitas SP. Towards integral utilization of grape pomace from winemaking process: A review. Waste Manag. p. 581–594 2017.
2 Christ KL and Burritt RL. Critical environmental concerns in wine production: An integrative review. J. Clean. Prod. Elsevier; p. 232–242 2013.
3 Bavaresco L, Fregoni C, Zeller De Macedo Basto Gonçalves MI Van, and Vezzulli S. Physiology & molecular biology of grapevine stilbenes: An update. Grapevine Molecular Physiology and Biotechnology: Second Edition Springer Netherlands; p. 341–364 2009.
4 Ruiz-García Y. Elicitores: una herramienta para incrementar el color y el aroma de uvas y vinos. [Murcia-Spain]: (Tesis Doctoral). Universidad de Murcia. Murcia-España; 2014.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Paladines-Quezada Diego F.1, Giménez-Banón1, Moreno-Olivares Juan D.1, Gómez-Martínez José C.1, Cebrián-Pérez Ana1, Fernández-Fernández José I.1, Bleda-Sánchez Juan A.1 and Gil-Munoz Rocío

¹Instituto Murciano de Investigación y Desarrollo Agrario y Medioambiental (IMIDA)

Contact the author

Keywords

bioproducts, revalue, sustainable, resveratrol, health.

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Grapevine yield-gap: identification of environmental limitations by soil and climate zoning in Languedoc-Roussillon region (south of France)

Grapevine yield has been historically overlooked, assuming a strong trade-off between grape yield and wine quality. At present, menaced by climate change, many vineyards in Southern France are far from the quality label threshold, becoming grapevine yield-gaps a major subject of concern. Although yield-gaps are well studied in arable crops, we know very little about grapevine yield-gaps. In the present study, we analysed the environmental component of grapevine yield-gaps linked to climate and soil resources in the Languedoc Roussillon. We used SAFRAN data and IGP Pays d’Oc wine yields from 2010 to 2018. We selected climate and soil indicators proving to have a significant effect on average wine yield-gaps at the municipality scale. The most significant factors of grapevine yield were the Soil Available Water Capacity; followed by the Huglin Index and the Climatic Dryness Index. The Days of Frost; the Soil pH; and the Very Hot Days were also significant. Then, we clustered geographical zones presenting similar indicators, facilitating the identification of resources yield-gaps. We discussed the number of zones with the experts of IGP Pays d’Oc label, obtaining 7 zones with similar limitations for grapevine yield. Finally, we analysed the main resources causing yield-gaps and the grapevine varieties planted on each zone. Mapping grapevine resource yield-gaps are the first stage for understanding grapevine yield-gaps at the regional scale.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

Influence of agronomic practices in soil water content in mid-mountain vineyards

In the context of LIFE project MIDMACC (LIFE18 CCA/ES/001099), several pilots have been installed in vineyards in mid mountain areas of Catalonia (NE Spain) to test well stablished agronomic practices to increase the adaptation of Mediterranean mid mountain to climate change. Soil water content (SWC) at three different depths (15, 30 and 45cm) was measured in continuum from August 2020. One pilot (WC) included a well-established green cover (GC), a new GC (NC) and a conventional soil management (CM, tilling+herbicides). NC presented an intermediate state between WC and CM, responding similarly to CM in autumn but quickly reaching similar SWC to WC, then following the same evolution till next spring, with CM presenting lower values along autumn and winter. Then vegetation activation decreased SWC in all plots, (much slower in CM, lacking GC). Sensibility to spring rains is again intermediate for NC, which joins SWC evolution of CM by the end of spring till next autumn. It is expected that NC will resemble WC more and more as its GC develops. In the pilot combining vine training (VSP vs Gobelet) and hillside management (slope vs terrace), no clear pattern could be related with these conditions. However, both terraces seem to be more sensitive to spring rains. A third pilot included new vineyards (7 and 1 year old). In the new vineyard (N), higher canopy development, a spontaneous green cover and row straw resulted in a slower SWC dynamic, not so sensitive to rains but conserving more soil water in spring and most of summer, even with presumably a higher water extraction by vines. In the newest vineyard (VN) the deepest sensor is still sensitive to rain events all over the year and SWC is always highest at this depth, revealing small water capture by vines.

Teasing apart terroir: the influence of management style on native yeast communities within Oregon wineries and vineyards

Newer sequencing technologies have allowed for the addition of microbes to the story of terroir. The same environmental factors that influence the phenotypic expression of a crop also shape the composition of the microbial communities found on that crop. For fermented goods, such as wine, that microbial community ultimately influences the organoleptic properties of the final product that is delivered to customers. Recent studies have begun to study the biogeography of wine-associated microbes within different growing regions, finding that communities are distinct across landscapes. Despite this new knowledge, there are still many questions about what factors drive these differences. Our goal was to quantify differences in yeast communities due to management style between seven pairs of conventional and biodynamic vineyards (14 in total) throughout Oregon, USA. We wanted to answer the following questions: 1) are yeast communities distinct between biodynamic vineyards and conventional vineyards? 2) are these differences consistent across a large geographic region? 3) can differences in yeast communities be tied to differences in metabolite profiles of the bottled wine? To collect our data we took soil, bark, leaf, and grape samples from within each vineyard from five different vines of pinot noir. We also collected must and a 10º brix sample from each winery. Using these samples, we performed 18S amplicon sequencing to identify the yeast present. We then used metabolomics to characterize the organoleptic compounds present in the bottled wine from the blocks the year that we sampled. We are actively in the process of analysing our data from this study.

Adaptability of grapevines to climate change: characterization of phenology and sugar accumulation of 50 varieties, under hot climate conditions

Climate is the major factor influencing the dynamics of the vegetative cycle and can determine the timing of phenological periods. Knowledge of the phenology of varieties, their chronological duration, and thermal requirements, allows not only for the better management of interventions in the vineyard, but also to predict the varieties’ behaviour in a scenario of climate change, giving the wine producer the possibility of selecting the grape varieties that are best adapted to the climatic conditions of a certain terroir. In 2014, Symington Family Estates, Vinhos, established two grape variety libraries in two different places with distinctive climate conditions (Douro Superior, and Cima Corgo), with the commitment of contributing to a deeper agronomic and oenological understanding of some grape varieties, in hot climate conditions. In these research vineyards are represented local varieties that are important in the regional and national viticulture, but also others that have over time been forgotten — as well as five international reference cultivars. From 2017 to 2021, phenological observations have been made three times a week, following a defined protocol, to determine the average dates of budbreak, flowering and veraison. With the climate data of each location, the thermal requirements of each variety and the chronological duration of each phase have been calculated. During maturation, berry samples have been gathered weekly to study the dynamics of sugar accumulation, between other parameters. The data was analysed applying phenological and sugar accumulation models available in literature. The results obtained show significant differences between the varieties over several parameters, from the chronological duration and thermal requirements to complete the various stages of development, to the differences between the two locations, confirming the influence of the climate on phenology and the stages of maturation, in these specific conditions.