IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The effect of wine matrix on the initial release of volatile compounds and their evolution in the headspace

The effect of wine matrix on the initial release of volatile compounds and their evolution in the headspace

Abstract

There is evidence in the literature that non-volatile wine matrix can modify the release and therefore the perception of the compounds involved in wine aroma [1-3]. The aim of the present study is to make an estimation of the nature of these changes by using a standard volatile composition added to different real wine matrices and then analyze the headspace above them.The analytical methodology is based on a previously developed DHS-TD-GC-MS method [4]. This analytical method provides a snapshot of the contents in wine vapors and allows a better understanding of the headspace profile changes. To study the influence of the wine matrix on the release of volatile compounds, the non-volatile matrix from six different wines was isolated and all volatile compounds removed. The non-volatile matrices were used to reconstitute the six original wines but this time the volatile composition was a standard aroma solution (15 volatile compounds of different chemical families) and the same alcoholic content. The headspaces of the reconstituted wines and a model wine (12% vol. ethanol, pH 3.5) were analyzed and compared at two different moments: just after wine pouring (t=0 min) and after 10 min with glass shaking (t=10 min). The analyses were triplicated for each model wine. Also, free and total sulfur dioxide, total polyphenol index, total acidity, pH, dry mass and contents on copper, iron and zinc were determined for each wine matrix.The data collected was studied according to the time spent after wine pouring, as this factor substantially modifies the headspace of most volatile compounds. The results of a one-way ANOVA to assess the influence of the wine matrix on the initial headspace composition showed significant differences for all compounds except ethyl decanoate. Dimethyl sulfide presented marked differences among wines matrices and a significant linear anti-correlation with the copper content of the matrices. Esters showed a similar trend in the release across wine matrices, although one wine was consistently releasing lower contents of ethyl esters. Butyric and hexanoic acids were the compounds with more marked differences in release, although other compounds like β-damascenone also displayed significant differences according to the wine matrix. The variation on the release of more polar and heavier compounds, like linalool, 4-ethylphenol or vanillin in the studied matrices was more similar to that of the model wine. Only in the matrix of a young red wine a salting-out effect was detected. The data obtained in this work proves that the same volatile composition in the liquid phase of very dissimilar non-volatile wine matrices produces a headspace profile above the wines that can be significantly different and, therefore, can undoubtedly influence the perception of wine aroma.

References

[1] D.-M. Jung, S.E. Ebeler, Headspace Solid-Phase Microextraction Method for the Study of the Volatility of Selected Flavor Compounds, (2003) 6.
[2] M.-P. Sáenz-Navajas, E. Campo, L. Culleré, P. Fernández-Zurbano, D. Valentin, V. Ferreira, Effects of the Nonvolatile Matrix on the Aroma Perception of Wine, J. Agric. Food Chem. 58 (2010) 5574–5585. https://doi.org/10.1021/jf904377p.
[3] J.J. Rodríguez-Bencomo, C. Muñoz-González, I. Andújar-Ortiz, P.J. Martín-Álvarez, M.V. Moreno-Arribas, M.Á. Pozo-Bayón, Assessment of the effect of the non-volatile wine matrix on the volatility of typical wine aroma compounds by headspace solid phase microextraction/gas chromatography analysis, J. Sci. Food Agric. 91 (2011) 2484–2494. https://doi.org/10.1002/jsfa.4494.
[4] Y. Wen, R. Lopez, V. Ferreira, An automated gas chromatographic-mass spectrometric method for the quantitative analysis of the odor-active molecules present in the vapors emanated from wine, J. Chromatogr. A. 1534 (2018) 130–138. https://doi.org/10.1016/j.chroma.2017.12.064.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Lopez Ricardo¹, Wen Yan¹and Ferreira Vicente¹

¹Laboratory for Aroma Analysis and Enology, Instituto Agroalimentario de Aragón (IA2), Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza

Contact the author

Keywords

headspace, aroma release, flavor-matrix interactions, wine, GC-MS

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Evolution of the amino acids content through grape ripening: Effect of foliar application of methyl jasmonate with or without urea

The parameters that determine the grape quality, and therefore the optimal harvest time, suffer variations during berry ripening, related to climate change, with the widely known problem of the gap between technological and phenolic maturities. However, there are few studies about its incidence on grape nitrogen composition. For this reason, the use of an elicitor, methyl jasmonate (MeJ), alone or with urea, is proposed as a tool to reduce climatic decoupling, allowing to establish the harvest time in order to achieve the optimum grape quality. The aim was to study the effect of MeJ and MeJ+Urea foliar applications on the evolution of Tempranillo amino acids content throughout the grape maturation. Three treatments were foliarly applied, at veraison and 7 days later: control (water), MeJ (10 mM) and MeJ+Urea (10 mM+6 kg N/ha). Grape samples were taken at five stages of maturation: day before the first and second applications, 15 days after the second application (pre-harvest), harvest day, and 15 days after harvest (post-harvest). The amino acids analysis of the samples was carried out by HPLC. Results showed that the evolution of amino acids was similar regardless of the treatment; however, foliar applications influenced the nitrogen compounds content, i.e., there was no qualitative effect but quantitative one. Most of the amino acids reached their maximum concentration in pre-harvest, being higher in grapes from the treatments than in the control. In general, no differences in grape amino acids content were observed between MeJ and MeJ+Urea treatments. Foliar applications with MeJ and MeJ+Urea enhanced the grape amino acids content, without affecting their profile, helping to optimize their quality and allowing to establish a more complete grape ripening standard. Therefore, MeJ and MeJ+Urea foliar applications can be a simple agronomic practice, which has shown promising results in order to enhance the grape quality.

Current climate change in the Oplenac wine-growing district (Serbia)

Serbian autochthonous vine varieties Smederevka (for white wines) and Prokupac (for rosé and red wines) are the primary representatives of typical characteristics of wines and terroir of numerous wine-growing areas in Serbia. In the past, these varieties were the leading vine varieties, however, as the result of globalization of winemaking and the trend of consumption of wines from widely prevalent vine varieties, they were replaced by introduced international varieties. Smederevka and Prokupac vine varieties are characterized by later time of grape ripening, and relative sensitivity to low temperatures. Climate conditions can be a restrictive factor for production of high-quality grapes and wine and for the spatial spreading of these varieties in hilly continental wine-growing areas.
This paper focuses on the spatial analysis of changes of main climate parameters, in particular, analysis of viticultural bioclimatic indices that were determined for the purposes of viticulture zoning of wine-growing areas in the period 1961-2010, and those same parameters determined for the current, that is, referential climate period (1988-2017). Results of the research, that is, analysis of climate changes indicate that the majority of examined climate parameters in the Oplenac wine-growing district improved from the perspective of Smederevka and Prokupac vine varieties. These studies of climate conditions indicate that changes of analyzed climate parameters, that is, bioclimatic indices will be favorable for cultivation of varieties with later grape ripening times and those more sensitive to low temperatures, such as the autochthonous vine varieties Smederevka and Prokupac, therefore, it is recommended to producers to more actively plant vineyards with these varieties in the territory of the Oplenac wine-growing district.

Climate modeling at local scale in the Waipara winegrowing region in the climate change context

In viticulture, a warming climate can have a very significant impact on grapevine development and therefore on the quality and characteristics of wines across different spatial scales, ranging from global to local. In order to adapt wine-growing to climate change, global climate models can be used to define future scenarios, but only at the scale of major wine regions. Despite the huge progress made over the last ten years in terms of the spatial resolution of climate models (now downscaled to a few square kilometres), they are not yet sufficiently precise to account for the local climate variability associated with such parameters as local topography, in spite of these parameters being decisive for vine and wine characteristics. This study describes a method to downscale future climate scenarios to vineyard scale. Networks of data loggers have been used to collect air temperature at canopy level in the Waipara winegrowing region (New Zealand) over five growing seasons. These measurements allow the creation of fine-scale geostatistical models and maps of temperature (at 100 m resolution) for the growing season. In order to model climate change at pilot site scale, these geostatistical models have been combined with regional climate change predictions for the periods 2031-2050 and 2081-2100 based on the RCP8.5 climate change scenario. The integration of local climate variability with regionalized climate change simulations allows assessment of the impacts of climate change at the vineyard scale. The improved knowledge gained using this methodology results from the increased horizontal resolution that better addresses the concerns of winegrowers. The results provide the local winegrowers with information necessary to understand current processes, as well as historical and future viticulture trends at the scale of their site, thereby facilitating decisions about future response strategies.

Vineyards and clay minerals: multi-technique analytical approach and correlations with soil properties

Purpose of this research is to quantitatively assess the mineral component of vineyard soils, with particular attention to the mineralogical analysis of clays, which represent an element of high importance in the vineyard culture as well as in general agriculture. An X-ray diffraction (XRD) / thermogravimetric (TG) multi-technique analytical approach was developed, tested on soil samples taken from vineyards around the world. This codified analytical procedure was necessary to obtain precise qualitative and quantitative mineralogical data, globally comparable to distinguish the geopedological identity of the vineyards. Soil samples from vineyards of various locations were analysed, in very different geological conditions. The bulk-rock quantitative phase analysis (QPA) was obtained by the Rietveld method while the detailed composition of the clay-sized fraction was determined by modelling of the oriented X-ray diffraction patterns. The research provided a precise classification of the mineral component of soils, distinguishing the mineral phases of the clays and the so-called mixed-layer clay minerals. We found that the content in mixed layers can be directly correlated with the water retention and the cation exchange capacity ​​of the soil, while the presence of other clayey minerals and phyllosilicates in this research did not affect this CEC parameter, which codes the fertility level of the soils. The study demonstrates that terroir, in particular soils formed in complex or very different geological conditions, can only be effectively interpreted by properly analysing its mineral phases, in particular the mixed-layer clay component. These are characteristic abiotic ecological indicators, which may have specific eco-physiological influences on the plant.

Climate change impacts: a multi-stress issue

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.