IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The effect of wine matrix on the initial release of volatile compounds and their evolution in the headspace

The effect of wine matrix on the initial release of volatile compounds and their evolution in the headspace

Abstract

There is evidence in the literature that non-volatile wine matrix can modify the release and therefore the perception of the compounds involved in wine aroma [1-3]. The aim of the present study is to make an estimation of the nature of these changes by using a standard volatile composition added to different real wine matrices and then analyze the headspace above them.The analytical methodology is based on a previously developed DHS-TD-GC-MS method [4]. This analytical method provides a snapshot of the contents in wine vapors and allows a better understanding of the headspace profile changes. To study the influence of the wine matrix on the release of volatile compounds, the non-volatile matrix from six different wines was isolated and all volatile compounds removed. The non-volatile matrices were used to reconstitute the six original wines but this time the volatile composition was a standard aroma solution (15 volatile compounds of different chemical families) and the same alcoholic content. The headspaces of the reconstituted wines and a model wine (12% vol. ethanol, pH 3.5) were analyzed and compared at two different moments: just after wine pouring (t=0 min) and after 10 min with glass shaking (t=10 min). The analyses were triplicated for each model wine. Also, free and total sulfur dioxide, total polyphenol index, total acidity, pH, dry mass and contents on copper, iron and zinc were determined for each wine matrix.The data collected was studied according to the time spent after wine pouring, as this factor substantially modifies the headspace of most volatile compounds. The results of a one-way ANOVA to assess the influence of the wine matrix on the initial headspace composition showed significant differences for all compounds except ethyl decanoate. Dimethyl sulfide presented marked differences among wines matrices and a significant linear anti-correlation with the copper content of the matrices. Esters showed a similar trend in the release across wine matrices, although one wine was consistently releasing lower contents of ethyl esters. Butyric and hexanoic acids were the compounds with more marked differences in release, although other compounds like β-damascenone also displayed significant differences according to the wine matrix. The variation on the release of more polar and heavier compounds, like linalool, 4-ethylphenol or vanillin in the studied matrices was more similar to that of the model wine. Only in the matrix of a young red wine a salting-out effect was detected. The data obtained in this work proves that the same volatile composition in the liquid phase of very dissimilar non-volatile wine matrices produces a headspace profile above the wines that can be significantly different and, therefore, can undoubtedly influence the perception of wine aroma.

References

[1] D.-M. Jung, S.E. Ebeler, Headspace Solid-Phase Microextraction Method for the Study of the Volatility of Selected Flavor Compounds, (2003) 6.
[2] M.-P. Sáenz-Navajas, E. Campo, L. Culleré, P. Fernández-Zurbano, D. Valentin, V. Ferreira, Effects of the Nonvolatile Matrix on the Aroma Perception of Wine, J. Agric. Food Chem. 58 (2010) 5574–5585. https://doi.org/10.1021/jf904377p.
[3] J.J. Rodríguez-Bencomo, C. Muñoz-González, I. Andújar-Ortiz, P.J. Martín-Álvarez, M.V. Moreno-Arribas, M.Á. Pozo-Bayón, Assessment of the effect of the non-volatile wine matrix on the volatility of typical wine aroma compounds by headspace solid phase microextraction/gas chromatography analysis, J. Sci. Food Agric. 91 (2011) 2484–2494. https://doi.org/10.1002/jsfa.4494.
[4] Y. Wen, R. Lopez, V. Ferreira, An automated gas chromatographic-mass spectrometric method for the quantitative analysis of the odor-active molecules present in the vapors emanated from wine, J. Chromatogr. A. 1534 (2018) 130–138. https://doi.org/10.1016/j.chroma.2017.12.064.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Lopez Ricardo¹, Wen Yan¹and Ferreira Vicente¹

¹Laboratory for Aroma Analysis and Enology, Instituto Agroalimentario de Aragón (IA2), Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza

Contact the author

Keywords

headspace, aroma release, flavor-matrix interactions, wine, GC-MS

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Sustaining wine identity through intra-varietal diversification

With contemporary climate change, cultivated Vitis vinifera L. is at risk as climate is a critical component in defining ecologically fitted plant materiel. While winegrowers can draw on the rich diversity among grapevine varieties to limit expected impacts (Morales-Castilla et al., 2020), replacing a signature variety that has created a sense of local distinctiveness may lead to several challenges. In order to sustain wine identity in uncertain climate outcomes, the study of intra-varietal diversity is important to reflect the adaptive and evolutionary potential of current cultivated varieties. The aim of this ongoing study is to understand to what extent can intra-varietal diversity be a climate change adaptation solution. With a focus on early (Sauvignon blanc, Riesling, Grolleau, Pinot noir) to moderate late (Chenin, Petit Verdot, Cabernet franc) ripening varieties, data was collected for flowering and veraison for the various studied accessions (from conservatory plots) and clones. For these phenological growing stages, heat requirements were established using nearby weather stations (adapted from the GFV model, Parker et al., 2013) and model performances were verified. Climate change projections were then integrated to predict the future behaviour of the intra-varietal diversity. Study findings highlight the strong phenotypic diversity of studied varieties and the importance of diversification to enhance climate change resilience. While model performances may require improvements, this study is the first step towards quantifying heat requirements of different clones and how they can provide adaptation solutions for winegrowers to sustain local wine identity in a global changing climate. As genetic diversity is an ongoing process through point mutations and epigenetic adaptations, perspective work is to explore clonal data from a wide variety of geographic locations.

Assessment of the impact of actions in the vineyard and its surrounding environment on biodiversity in Rioja Alavesa (Spain)

Traditional viticulture areas have experienced in the last decades an intensification of field practices, linked to an increased use of fertilisers and phytosanitary products, and to a more intensive mechanization and uniformization of the landscape. This change in management has sometimes led to higher rates of soil erosion andloss of soil structure, fertility decline, groundwater contamination, and to an increased pressure of pests and diseases. Additionally, intensification usually leads to a simplification of landscapes, of particular concern in prestigious wine grape regions where the economical revenue encourages the conversion of land use from natural habitats to high value wine grape production. To revert this trend, it is necessary that growers implement actions that promote biodiversity in their vineyards. The aim of this study is to assess the impact of the implementation of cover crops, vegetational corridors, dry stone walls and vineyard biodiversity hotspots estimated through the study of arthropods. The work has been carried out in four vineyards in Rioja Alavesa belonging to Ostatu winery, where these infrastructures were implemented in 2020. The presence and diversity of arthropods was studied by capturing them at different times in the season and at different distances from the infrastructure using pit-fall traps in the soil and yellow, white and blue chromatic traps at the canopy level. This is a preliminary study in which all adult insects were sorted to the taxonomic level of order and Coleoptera were classified to morphospecies. The results obtained show that there is a relationship between the basic characteristics of the vineyard and the arthropods captured, with a positive effect, although also dependent on the vineyard, of the presence of infrastructure.

A blueprint for managing vine physiological balance at different spatial and temporal scales in Champagne

In Champagne, the vine adaptation to different climatic and technical changes during these last 20 years can be seen through physiological balance disruptions. These disruptions emphasize the general grapevine decline. Since the 2000s, among other nitrogen stress indicators, the must nitrogen has been decreasing. The combination of restricted mineral fertilizers and herbicide use, the growing variability of spring rainfall, the increasing thermal stress as well as the soil type heterogeneity are only a few underlying factors that trigger loss of physiological balance in the vineyards. It is important to weigh and quantify the impact of these factors on the vine. In order to do so, the Comité Champagne uses two key-tools: networking and modelization. The use of quantitative and harmonized ecophysiological indicators is necessary, especially in large spatial scales such as the Champagne appellation. A working group with different professional structures of Champagne has been launched by the Comité Champagne in order to create a common ecophysiology protocol and thus monitor the vine physiology, yearly, around 100 plots, with various cultural practices and types of soil. The use of crop modelling to follow the vine physiological balance within different pedoclimatic conditions enables to understand the present balance but also predict the possible disruptions to come in future climatic scenarios. The physiological references created each year through the working group, benefit the calibration of the STICS model used in Champagne. In return, the model delivers ecophysiology indicators, on a daily scale and can be used on very different types of soils. This study will present the bottom-up method used to give accurate information on the impacts of soil, climate and cultural practices on vine physiology.

Investigating the impact of grape exposure and UV radiations on rotundone in Vitis vinifera L. Tardif grapes under field trial conditions

Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing sustainable viticultural strategies to enhance rotundone accumulation or limit its degradation. It was recently proposed that ultraviolet (UV) radiations could stimulate rotundone production. The aim of this study was to investigate under field trial conditions the impact of grape exposure and UV treatments on rotundone in Vitis vinifera L. Tardif, an almost extinct grape variety from south-west France that can express particularly high rotundone levels. Four different treatments were compared in 2021 to a control treatment using a randomised complete block design with three replications per treatment. Grape exposure was manipulated through early or late defoliation. Leaf and laterals shoots were removed at Eichorn Lorenz growth stages 32 or 34 on the morning-sun side of the canopy. During grape maturation, UV radiations were either reduced by 99% by installing UV radiation-shielding sheets, or applied four times using the Boxilumix™ non thermal device (Asclepios Tech, Tournefeuille) with the aim of activating plant signalling pathway. Loggers displayed in solar radiation shields were used to assess the effect of such shielding sheets on air temperature within the bunch zone. The composition of grapes subjected to these treatments will be soon analysed for their rotundone content and basic classical laboratory analyses. Grapes will be harvested to elaborate wines under standardized small-scale vinification conditions (60kg) that will be assessed by a trained sensory panel.

Pruned vine biomass exclusion from a clay loam vineyard soil – examining the impact on physical/chemical properties

The wine industry worldwide faces increasing challenges to achieve sustainable levels of carbon emission mitigation. This project seeks to establish the feasibility of harvesting winter pruned vineyard biomass (PVB) for potential use in carbon footprint reduction, through its use as a renewable biofuel for energy production. In order to make this recommendation, technical issues such as the potential environmental impact, chemical composition and fuel suitability, and logistical challenges of harvesting biomass needs to be understood to compare with the results from similar studies. Of particular interest is the role PVB plays as a carbon source in vineyard soils and what effect annual removal might have on soil carbon sequestration. A preliminary trial was established in the Waite Campus vineyard (University of Adelaide) to test current management strategies. Vines are grown in a Eutrophic, Red Dermosol clay loam soil with well managed midrow swards. A comparison was undertaken of mid-row treatments in two 0.25 Ha blocks (Shiraz and Semillon), including annual cultivation for seed bed preparation, the deliberate exclusion of PVB (25 years) and incorporation of PVB (13 years) at an average of 3.4 and 5.5 Mg/Ha-1 for Shiraz and Semillon respectively. In both 0-10cm and 10-30cm soil core sample depths, combined soil carbon % measures in the desired range of 1.80 to 3.50, were not significantly different between treatments or cultivars and yielded an estimated 42 Mg/ha-1 of sequestered soil carbon. Other key physical and chemical measures were likewise not significantly different between treatments. Preliminary results suggest that in a temperate zone vineyard, managed such as the one used in this study, there is no long term negative impact on soil carbon sequestration through removing PVB. This implies that growers could confidently harvest PVB for use in several end fates including as a bio fuel.