IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Evaluation of “Accentuated cut edges” technique on the release of varietal thiols and their precursors in Shiraz and Sauvignon blanc wine production

Evaluation of “Accentuated cut edges” technique on the release of varietal thiols and their precursors in Shiraz and Sauvignon blanc wine production

Abstract

Accentuated cut edges (ACE) is a novel grape crushing technique used sequentially after a conventional crusher to increase the extraction rate and content of polyphenolics, as shown for Pinot noir wine. This inspired us to apply the technique during Shiraz and Sauvignon blanc winemaking, primarily to assess its impact on the extraction of varietal thiol precursors in grape must/juice and formation of varietal thiols in the resultant wines. Other variables were jointly studied, including skin contact time and water addition to Shiraz grape must, and yeast strain and malolactic fermentation (MLF) for Sauvignon blanc. Varietal thiol precursors (3-S-glutathionylhexan-1-ol (GSH-3-SH), 3-S-cysteinylhexan-1-ol (Cys-3-SH)) and varietal thiols derivatised with 4,4′-dithiodipyridine were separately determined using high performance liquid chromatography tandem mass spectrometry. The concentrations of GSH-3-SH and Cys-3-SH in Shiraz grape must and varietal thiols (3-sulfanylhexan-1-ol (3-SH) and 3-sulfanylhexyl acetate (3-SHA)) in Shiraz wine were not significantly affected by grape crushing method, but a shorter skin contact time (3 days) during fermentation significantly increased 3-SH compared to a 6-day treatment. For Sauvignon blanc juice, the evolution profile of GSH-3-SH in the ACE treatment during cold maceration showed an increase from 313 µg/L at the beginning of maceration to the maximum content of 514 µg/L within 9 h, in stark comparison to the conventional crushing treatment, which initially contained 315 µg/L and reached a maximum of 382 µg/L at the end of the cold maceration period (21 h). The evolution profile of Cys-3-SH was similar to that of the GSH-3-SH, yielding 16 µg/L in ACE and 7 µg/L in conventional crushing at the end of maceration. Varietal thiols were determined in Sauvignon blanc wine, including 4-methyl-4-sulfanylpentan-2-one (4-MSP) and enantiomers of 3-SH and 3-SHA, with concentrations of 76–188 ng/L for 4-MSP, 456–864 ng/L and 434 850 ng/L for (3S)-3-SH and (3R)-3-SH, respectively, and 13–29 ng/L and 6–15 ng/L for (3S)-3-SHA and (3R)-3-SHA, respectively. Three-way analysis of variance revealed that their concentrations were significantly affected by the interaction effects of crushing method, yeast strain, and MLF, with ACE significantly increasing their concentrations compared to conventional crushing. Differences were also observed for yeast strain and MLF, with VIN13 yeast strain leading to greater amounts of 3-SH and 3-SHA enantiomers but less 4-MSP than Sauvy, whereas MLF treatment afforded higher amounts of 3-SH enantiomers and 4-MSP but lower levels of 3-SHA enantiomers than those without MLF. The molar conversion yield from the sum of GSH-3-SH and Cys-3-SH to the sum of 3-SH and 3-SHA was relatively low – ranging from 0.65% to 1.01% – and was significantly affected by two-way interaction effects, with VIN13, MLF, and ACE significantly increasing the conversion yield by up to 0.2%.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Wang Xingchen1, Capona Dimitra L.1, Roland Aurélie2, Kang Wenyu1 and Jeffery David W.1

1Department of Wine Science and Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia
2SPO, Univ Montpellier, INRAE, Institut Agro.

Contact the author

Keywords

Accentuated cut edges; varietal thiols; precursors; three-way analysis of variance; Sauvignon blanc

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Combining effect of leaf removal and natural shading on grape ripening under two irrigation strategies in Manto negro (Vitis vinifera L.)

The increasingly frequent heat waves during grape ripening pose challenges for high quality wine grape production. Defoliation is a common practice that can improve the control of diseases in bunches, but also it increases the exposure to sunlight. Grapes exposed to solar radiation reach temperatures over the optimum for berry development and maturation. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 using Manto negro wine grapes to study the effect of applied irrigation and different light exposure levels on grape quality. Two irrigation treatments were imposed based on the frequency and amount of water doses in a four-block experimental vineyard at Bodega Ribas (Mallorca). Three light exposure treatments were randomly applied in each irrigation plot. The light treatments included exposed clusters from pea size, non-exposed clusters, and shaded clusters after softening. Leaf area index and canopy porosity was estimated every 2 weeks. Midday leaf water potential was measured weekly. Additionally, apparent electrical conductivity was measured between rows to estimate the soil water content variability. Light and temperature sensors were installed at the bunch level to quantify the differences in bunch temperature and light intensity among treatments. The effect of irrigation and cluster light exposure on berry weight, TSS, TA, malic acid, tartaric acid, K+, and pH were analysed at 5 moments along grape ripening. During different heat waves, the natural shading technique decreased the maximum bunch temperature around 10 °C respect to the exposed bunches in both irrigation strategies. The combination of defoliation and shading techniques after softening decreased TSS at harvest and affected most of the quality parameters during the last stages of ripening, showing an interesting technique to delay ripening in warm viticulture areas.

Terroir analysis and its complexity

Terroir is not only a geographical site, but it is a more complex concept able to express the “collective knowledge of the interactions” between the environment and the vines mediated through human action and “providing distinctive characteristics” to the final product (OIV 2010). It is often treated and accepted as a “black box”, in which the relationships between wine and its origin have not been clearly explained. Nevertheless, it is well known that terroir expression is strongly dependent on the physical environment, and in particular on the interaction between soil-plant and atmosphere system, which influences the grapevine responses, grapes composition and wine quality. The Terroir studying and mapping are based on viticultural zoning procedures, obtained with different levels of know-how, at different spatial and temporal scales, empiricism and complexity in the description of involved bio-physical processes, and integrating or not the multidisciplinary nature of the terroir. The scientific understanding of the mechanisms ruling both the vineyard variability and the quality of grapes is one of the most important scientific focuses of terroir research. In fact, this know-how is crucial for supporting the analysis of climate change impacts on terroir resilience, identifying new promised lands for viticulture, and driving vineyard management toward a target oenological goal. In this contribution, an overview of the last findings in terroir studies and approaches will be shown with special attention to the terroir resilience analysis to climate change, facing the use and abuse of terroir concept and new technology able to support it and identifying the terroir zones.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Climate change impacts: a multi-stress issue

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).