IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Trace-level analysis of phosphonate in wine and must by ion chromatography with inductively coupled plasma mass spectrometry (IC-ICP-MS).

Trace-level analysis of phosphonate in wine and must by ion chromatography with inductively coupled plasma mass spectrometry (IC-ICP-MS).

Abstract

Phosphonic acid and especially potassium dihydrogen phosphonate are widely used to restrain the ubiquitous pressure of grapevine downy mildew in viticulture. Nevertheless, phosphonic acid and its derivatives have been banned in organic viticulture in October 2013, because they have been classified as plant protection products since then. This development has fueled the need for analytical methods for sensitive phosphonate determination. Current routine analysis of phosphonic acid is usually performed by ion chromatography with conductivity detection (IC-CD), which is not always sufficiently sensitive and specific. Furthermore, the quick polar pesticide evaluation method (QuPPe) of the European Reference Laboratory in combination with LC-MS/MS is well established for most polar pesticides. However, in case of phosphonic acid, issues regarding mass transitions and poor chromatographic resolution, can occur. Therefore, we sought to evaluate a new method based on IC separation coupled with ICP-MS detection as an alternative for previously described methods. By coupling an ICP-MS to an IC, non-phosphorus-containing, coeluting substances can be eliminated and thus a higher specificity can be achieved. Hence, this contribution highlights the development and validation of an IC-ICP-MS based workflow for the robust, sensitive and reliable determination of phosphonic acid at low µg/kg levels in wine and must. This method is then compared to the previous detection by CD and the advantages and disadvantages of each are briefly described. Quantification limits are 20 µg/kg or lower with % RSDs typically

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Otto Sören1, May Bianca2 and Schweiggert Ralf1

1Department of Beverage Research, Chair Analysis and Technology of Plant-based Foods, Geisenheim University
2Department of Enology, Chair Wine and Beverage Chemistry, Geisenheim University

Contact the author

Keywords

polar pesticides, IC-ICP-MS, IC-CD, phosphonic acid, organic viticulture

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Comparison between satellite and ground data with UAV-based information to analyse vineyard spatio-temporal variability

Currently, the greatest challenge for vine growers is to improve the yield and quality of grapes by minimizing costs and environmental impacts. This goal can be achieved through a better knowledge of vineyard spatial variability. Traditional platforms such as airborne, satellite and unmanned aerial vehicles (UAVs) solutions are useful investigation tools for vineyard site specific management.

A comprehensive ecological study of grapevine sensitivity to temperature; how terroir will shift under climate change

Fossil fuel combustion continues to drive increases in atmospheric carbon dioxide, consequently elevating the global annual mean temperature and specifically increasing the growing season temperatures in many of the world’s most important wine growing regions (IPCC 2014; Jones et al 2005). Grapes are sensitive to changes in growing season temperatures, and past models have shown a direct link between warming temperatures and earlier harvest dates (Cook and Wolkovich 2016). Globally, there have been shifts of 1-2 weeks for wine growing regions (Wolkovich et al 2017 and references within). The phenological shifts resulting from growing season temperature increases are documented internationally, and models predicting phenology using temperature are becoming more precise (Parker et al 2011).

Anthocyanin and trans-resveratrol accumulation is associated with abscisic acid and methyl jasmonicanthocyanin and trans-resveratrol accumulation is acid in berry skin of vitis vinifera L. Cvs. Malbec, Bonarda, Syrah, Cabernet sauvignon, and Pinot noir

Red grapes contain significant amounts of phenolic compounds, known to contribute to wine quality and to provide important health benefits. Berry skin phenolics can be elicited by plant hormones. The aim of this work was to increase the content of anthocyanins and trans-resveratrol in five red varieties cultured in Argentina: Malbec (M), Bonarda (B), Syrah (S), Cabernet Sauvignon (CS), and Pinot Noir (PN), in two different growing regions: Santa Rosa (SR) and Valle de Uco (VU), by applying a post-veraison hormonal treatment with abscisic acid (ABA) and methyl jasmonate (MeJA).

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.

Protein stabilization of white wines by stabilizing filtration: pilot studies

Protein stabilization is an important part of the winemaking process of white wines, and in this work we present the results of protein stabilization of different monovarietal wines (Xarel.lo, Chardonnay, and Muscat) by a continuous stabilizing filtration process using a column packed with zirconium oxide operating in a continuous regime in a closed loop at pilot scale.