IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Trace-level analysis of phosphonate in wine and must by ion chromatography with inductively coupled plasma mass spectrometry (IC-ICP-MS).

Trace-level analysis of phosphonate in wine and must by ion chromatography with inductively coupled plasma mass spectrometry (IC-ICP-MS).


Phosphonic acid and especially potassium dihydrogen phosphonate are widely used to restrain the ubiquitous pressure of grapevine downy mildew in viticulture. Nevertheless, phosphonic acid and its derivatives have been banned in organic viticulture in October 2013, because they have been classified as plant protection products since then. This development has fueled the need for analytical methods for sensitive phosphonate determination. Current routine analysis of phosphonic acid is usually performed by ion chromatography with conductivity detection (IC-CD), which is not always sufficiently sensitive and specific. Furthermore, the quick polar pesticide evaluation method (QuPPe) of the European Reference Laboratory in combination with LC-MS/MS is well established for most polar pesticides. However, in case of phosphonic acid, issues regarding mass transitions and poor chromatographic resolution, can occur. Therefore, we sought to evaluate a new method based on IC separation coupled with ICP-MS detection as an alternative for previously described methods. By coupling an ICP-MS to an IC, non-phosphorus-containing, coeluting substances can be eliminated and thus a higher specificity can be achieved. Hence, this contribution highlights the development and validation of an IC-ICP-MS based workflow for the robust, sensitive and reliable determination of phosphonic acid at low µg/kg levels in wine and must. This method is then compared to the previous detection by CD and the advantages and disadvantages of each are briefly described. Quantification limits are 20 µg/kg or lower with % RSDs typically


Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster


Otto Sören1, May Bianca2 and Schweiggert Ralf1

1Department of Beverage Research, Chair Analysis and Technology of Plant-based Foods, Geisenheim University
2Department of Enology, Chair Wine and Beverage Chemistry, Geisenheim University

Contact the author


polar pesticides, IC-ICP-MS, IC-CD, phosphonic acid, organic viticulture


IVAS 2022 | IVES Conference Series


Related articles…

Geochemistry of Vrbničko Polje (Croatia) winegrowing site

A multi-element pedo-geochemical survey was carried out in Vrbničko polje vineyards on the Krk Island, Croatia. This Mediterranean winegrowing site is famous by Žlahtina wine production.

Under-vine cover crops: impact on weed development, yield and grape composition

This study aims to evaluate the interest of using an under-vine cover crop as a sustainable management tool replacing herbicides or tillage to control weeds, evaluating its effects on yield and berry parameters in a semi-arid climate. 
The performance of Trifolium fragiferum as an under-vine cover crop was evaluated in 2018 and 2019 in a Merlot vineyard in

Effects of post-veraison irrigation dose on Cabernet-Sauvignon vines in a dry and warm season in Valencia, Spain

In the old-world viticulture, there is a common but most often not scientifically proved consideration that supplemental irrigation should detrimentally affect berry and wine composition. In the semi-arid

Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Le chargement et l’accumulation des sucres ainsi que la biosynthèse des phénols ont été étudiés sur la Syrah, dans le cadre d’un programme de recherche de paramètres qui permettraient de déterminer une ou plusieurs qualités de raisin en relation avec des styles de vins pour un terroir donné. La relation entre la dynamique d’accumulation des

The effects of calcite silicon-mediated particle film application on leaf temperature and grape composition of Merlot (Vitis vinifera L.) vines under different irrigation conditions

This study examined whether the application of calcite-silicon mediated particle film (CaPF) at veraison can mitigate a drought-induced increase in leaf temperature on grapevine, thus contributing to improved leaf functionality, yield and grape composition traits. A total of 48 five-year-old Merlot (Vitis vinifera L.)