IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Quantification of Eugenol in various matrixes from hybrids vines. Case study of Armagnac white spirits production

Quantification of Eugenol in various matrixes from hybrids vines. Case study of Armagnac white spirits production

Abstract

Nowadays, winemaking is dealing with great challenges, notably climate change, disease resistance and low pesticide inputs, desire for more sustainable agricultural productions and permanent changing of consumer preference. Trying to propose practice improvements, scientists are exploring vine hybridization a paradoxically old but still promising way to take up such challenges (Pedneault & Provost, 2016). Phylloxera crisis in Europe (XIXth century) was a crucial step for improving hybridization in grapevine. Unfortunately some of the wines produced then presented redibitory sensorial default and were finally excluded for getting the PDO (Protected Designation of Origin) wine label in France.However, one grape variety from Armagnac vineyard was maintained despite the ban: the Baco blanc, a complex hybrid of Vitis labrusca x Vitis riparia x Vitis vinifera. Baco was created to be a disease tolerant and productive vine of white wine intended for distillation (Baco, 1925).Various matrixes, from vine shoots to distillates, are available for analysis in Armagnac wine spirit production. It was noticed that Baco blanc samples (all matrixes included) had an atypical chemical profile. Indeed, eugenol, a phenylpropenic compound, usually known to be extracted by contact with oak, is significantly more concentrated in white spirits made with Baco than same products made with V.vinifera cultivars. Eugenol was also quantified in other hybrids (Vitis riparia-containing hybrids as Marechal Foch and Frontenac) wines but in lower concentrations than in Baco blanc wines (Sun et al., 2011).Eugenol has a clove aroma and a noticeable and well-known antiseptic action, a gustative impact including an anesthesic power. These observations raise many questions, the main ones being: “Is there a link between tolerance of Baco to diseases and the presence of eugenol?”; “What is the dynamic of eugenol levels during spirit making?”; “Is there a link between the eugenol presence and the typicity of Baco wine spirits ?”Trying to answer these questions eugenol quantification was carried out by a HS-SPME-GC-MS method using deuterium labelled eugenol as internal standard and a procedure adapted to each matrix. An enzymatic hydrolysis (β-glucosidase enzyme) with addition of citrate-phosphate buffer was performed for plant material, musts and wines. The main goal was to highlight the existence of two eugenol fractions : a free one and a bound one. Such eugenol « cartography » resulted in interesting observations. First a greater eugenol concentration and accumulation during maturation occurs in Baco blanc than in other V.vinifera cultivars tested (Ugni blanc and Folle blanche). Second using enzymes increases the eugenol content during first steps of winemaking. Third, eugenol amounts seem to increase along with the storage duration on lees (before distillation). Finally, alambic characteristics may influence the alcohol content which may also impact eugenol concentration.

References

Baco, F. (1925). Précis complet de viticulture moderne et de vinification : Mes meilleures vignes hybrides franco-americaines : leurs principaux caractères : les meilleurs moyens pour les multiplier, les planter, les tailler, les cultiver, les vinifier (Imprimeries Gounouilhou)
Pedneault, K., & Provost, C. (2016). Fungus resistant grape varieties as a suitable alternative for organic wine production : Benefits, limits, and challenges. Scientia Horticulturae, 208, 57-77. https://doi.org/10.1016/j.scienta.2016.03.016
Sun, Q., Gates, M. J., Lavin, E. H., Acree, T. E., & Sacks, G. L. (2011). Comparison of Odor-Active Compounds in Grapes and Wines from Vitis vinifera and Non-Foxy American Grape Species. Journal of Agricultural and Food Chemistry, 59(19), 10657-10664. https://doi.org/10.1021/jf2026204

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Hastoy Xavier1, Franc Céline1, Riquier Laurent1, Marchand-Marion Stéphanie1, Ségur Marie-Claude2, Fermaud Marc3 and De Revel Gilles1

1Université de Bordeaux, INRAE, Bordeaux INP, OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
2Bureau National Interprofessionnel de l’Armagnac (BNIA), 32800 Eauze, France
3INRAE, UMR SAVE, UMTSeven, Bordeaux Science Agro, ISVV, F-33882, Villenave d’Ornon, France 

Contact the author

Keywords

Phenylpropenes, Baco blanc, Hybrid vines, White wine spirits, Armagnac

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Rapid damage assessment and grapevine recovery after fire

There is increasing scientific consensus that climate changeis the underlying cause of the prolonged dry and hot conditions that have increased the risk of extreme fire weather in many countries around the world. In December 2019, a bushfire event occurred in the Adelaide Hills, South Australia where 25,000 hectares were burnt and in vineyards and surrounding areas various degrees of scorching and infrastructure damage occurred. The ability to coordinate and plan recovery after a fire event relies on robust and timely data. The current practice for measuring the scale and distribution of fire damage is to walk or drive the vineyard and score individual vines based on visual observation. The process is time consuming, subjective, or semi-quantitative at best. After the December 2019 fires, it took many months to access properties and estimate the area of vineyard damaged. This study compares the rapid assessment and mapping of fire damage using high-resolution satellite imagery with more traditional ground based measures. Satellite imagery tracking vineyard recovery in the season following the bushfire is being correlated to field assessments of vineyard productivity such as canopy health and development, fertility and carbohydrate storage. Canopy health in the seasons following the fires correlated to the severity of the initial fire damage. Severely damaged vines had reduced canopy growth, were infertile or had very low fertility as well as lower carbohydrate levels in buds and canes during dormancy, which reduced productivity in the seasons following the bushfire event. In contrast, vines that received minor damage were able to recover within 1-2 years. Tools that rapidly and affordably capture the extent and severity of damage over large vineyard area will allow producers, government and industry bodies to manage decisions in relation to fire recovery planning, coordination and delivery, improving the efficiency and effectiveness of their response.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.

VINIoT: Precision viticulture service for SMEs based on IoT sensors network

The main innovation in the VINIoT service is the joint use of two technologies that are currently used separately: vineyard monitoring using multispectral imaging and deployed terrain sensors. One part of the system is based on the development of artificial intelligence algorithms that are feed on the images of the multispectral camera and IoT sensors, high-level information on water stress, grape ripening status and the presence of diseases. In order to obtain algorithms to determine the state of ripening of the grapes and avoid losing information due to the diversity of the grape berries, it was decided to work along the first year 2020 at berry scale in the laboratory, during the second year at the cluster scale and on the last year at plot scale. Different varieties of white and red grapes were used; in the case of Galicia we worked with the white grape variety Treixadura and the red variety Mencía. During the 2020 and 2021 campaigns, multispectral images were taken in the visible and infrared range of: 1) sets of 100 grapes classifying them by means of densimetric baths, 2) individual bunches. The images taken with the laboratory analysis of the ripening stage were correlated. Technological maturity, pH, probable degree, malic acid content, tartaric acid content and parameters for assessing phenolic maturity, IPT, anthocyanin content were determined. It has been calculated for each single image the mean value of each spectral band (only taking into account the pixels of interest) and a correlation study of these values with laboratory data has been carried out. These studies are still provisional and it will be necessary to continue with them, jointly with the training of the machine learning algorithms. Processed data will allow to determine the sensitivity of the multispectral images and select bands of interest in maturation.

Soil, vine, climate change – what is observed – what is expected

To evaluate the current and future impact of climate change on Viticulture requires an integrated view on a complex interacting system within the soil-plant-atmospheric continuum under continuous change. Aside of the globally observed increase in temperature in basically all viticulture regions for at least four decades, we observe several clear trends at the regional level in the ratio of precipitation to potential evapotranspiration. Additionally the recently published 6th assessment report of the IPCC (The physical science basis) shows case-dependent further expected shifts in climate patterns which will have substantial impacts on the way we will conduct viticulture in the decades to come.
Looking beyond climate developments, we observe rising temperatures in the upper soil layers which will have an impact on the distribution of microbial populations, the decay rate of organic matter or the storage capacity for carbon, thus affecting the emission of greenhouse gases (GHGs) and the viscosity of water in the soil-plant pathway, altering the transport of water. If the upper soil layers dry out faster due to less rainfall and/or increased evapotranspiration driven by higher temperatures, the spectral reflection properties of bare soil change and the transport of latent heat into the fruiting zone is increased putting a higher temperature load on the fruit. Interactions between micro-organisms in the rhizosphere and the grapevine root system are poorly understood but respond to environmental factors (such as increased soil temperatures) and the plant material (rootstock for instance), respectively the cultivation system (for example bio-organic versus conventional). This adds to an extremely complex system to manage in terms of increased resilience, adaptation to and even mitigation of climate change. Nevertheless, taken as a whole, effects on the individual expressions of wines with a given origin, seem highly likely to become more apparent.

Modeling island and coastal vineyards potential in the context of climate change

Climate change impacts regional and local climates, which in turn affects the world’s wine regions. In the short term, these modifications rises issues about maintaining quality and style of wine, and in a longer term about the suitability of grape varieties and the sustainability of traditional wine regions. Thus, adaptation to climate change represents a major challenge for viticulture. In this context, island and coastal vineyards could become coveted areas due to their specific climatic conditions. In regions subject to warming, the proximity of the sea can moderate extremes temperatures, which could be an advantage for wine. However, coastal and island areas are particular prized spaces and subject to multiple pressures that make the establishment or extension of viticulture complex.
In this perspective, it seems relevant to assess the potentialities of coastal and island areas for viticulture. This contribution will present a spatial optimization model that tends to characterize most suitable agroclimatic patterns in historical or emerging vineyards according to different scenarios. Thanks to an in-depth bibliography a global inventory of coastal and insular vineyards on a worldwide scale has been realized. Relevant criteria have been identified to describe the specificities of these vineyards. They are used as input data in the optimization process, which will optimize some objectives and spatial aspects. According to a predefined scenario, the objectives are set in three main categories associated with climatic characteristics, vineyards characteristics and management strategies. At the end of this optimization process, a series of maps presents the different spatial configurations that maximize the scenario objectives.