IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Quantification of Eugenol in various matrixes from hybrids vines. Case study of Armagnac white spirits production

Quantification of Eugenol in various matrixes from hybrids vines. Case study of Armagnac white spirits production

Abstract

Nowadays, winemaking is dealing with great challenges, notably climate change, disease resistance and low pesticide inputs, desire for more sustainable agricultural productions and permanent changing of consumer preference. Trying to propose practice improvements, scientists are exploring vine hybridization a paradoxically old but still promising way to take up such challenges (Pedneault & Provost, 2016). Phylloxera crisis in Europe (XIXth century) was a crucial step for improving hybridization in grapevine. Unfortunately some of the wines produced then presented redibitory sensorial default and were finally excluded for getting the PDO (Protected Designation of Origin) wine label in France.However, one grape variety from Armagnac vineyard was maintained despite the ban: the Baco blanc, a complex hybrid of Vitis labrusca x Vitis riparia x Vitis vinifera. Baco was created to be a disease tolerant and productive vine of white wine intended for distillation (Baco, 1925).Various matrixes, from vine shoots to distillates, are available for analysis in Armagnac wine spirit production. It was noticed that Baco blanc samples (all matrixes included) had an atypical chemical profile. Indeed, eugenol, a phenylpropenic compound, usually known to be extracted by contact with oak, is significantly more concentrated in white spirits made with Baco than same products made with V.vinifera cultivars. Eugenol was also quantified in other hybrids (Vitis riparia-containing hybrids as Marechal Foch and Frontenac) wines but in lower concentrations than in Baco blanc wines (Sun et al., 2011).Eugenol has a clove aroma and a noticeable and well-known antiseptic action, a gustative impact including an anesthesic power. These observations raise many questions, the main ones being: “Is there a link between tolerance of Baco to diseases and the presence of eugenol?”; “What is the dynamic of eugenol levels during spirit making?”; “Is there a link between the eugenol presence and the typicity of Baco wine spirits ?”Trying to answer these questions eugenol quantification was carried out by a HS-SPME-GC-MS method using deuterium labelled eugenol as internal standard and a procedure adapted to each matrix. An enzymatic hydrolysis (β-glucosidase enzyme) with addition of citrate-phosphate buffer was performed for plant material, musts and wines. The main goal was to highlight the existence of two eugenol fractions : a free one and a bound one. Such eugenol « cartography » resulted in interesting observations. First a greater eugenol concentration and accumulation during maturation occurs in Baco blanc than in other V.vinifera cultivars tested (Ugni blanc and Folle blanche). Second using enzymes increases the eugenol content during first steps of winemaking. Third, eugenol amounts seem to increase along with the storage duration on lees (before distillation). Finally, alambic characteristics may influence the alcohol content which may also impact eugenol concentration.

References

Baco, F. (1925). Précis complet de viticulture moderne et de vinification : Mes meilleures vignes hybrides franco-americaines : leurs principaux caractères : les meilleurs moyens pour les multiplier, les planter, les tailler, les cultiver, les vinifier (Imprimeries Gounouilhou)
Pedneault, K., & Provost, C. (2016). Fungus resistant grape varieties as a suitable alternative for organic wine production : Benefits, limits, and challenges. Scientia Horticulturae, 208, 57-77. https://doi.org/10.1016/j.scienta.2016.03.016
Sun, Q., Gates, M. J., Lavin, E. H., Acree, T. E., & Sacks, G. L. (2011). Comparison of Odor-Active Compounds in Grapes and Wines from Vitis vinifera and Non-Foxy American Grape Species. Journal of Agricultural and Food Chemistry, 59(19), 10657-10664. https://doi.org/10.1021/jf2026204

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Hastoy Xavier1, Franc Céline1, Riquier Laurent1, Marchand-Marion Stéphanie1, Ségur Marie-Claude2, Fermaud Marc3 and De Revel Gilles1

1Université de Bordeaux, INRAE, Bordeaux INP, OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
2Bureau National Interprofessionnel de l’Armagnac (BNIA), 32800 Eauze, France
3INRAE, UMR SAVE, UMTSeven, Bordeaux Science Agro, ISVV, F-33882, Villenave d’Ornon, France 

Contact the author

Keywords

Phenylpropenes, Baco blanc, Hybrid vines, White wine spirits, Armagnac

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

A predictive model of spatial Eca variability in the vineyard to support the monitoring of plant status

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Effect of the commercial inoculum of arbuscular mycorrhiza in the establishment of a commercial vineyard of the cultivar “Manto negro

The favorable effect of symbiosis with arbuscular mycorrhizal fungi (AMF) has been known and studied since the 60s. Nowadays, many companies took the chance to start promoting and selling commercial inoculants of AMF, in order to be used as biofertilizers and encourage sustainable biological agriculture. However, the positive effect of these commercial biofertilizers on plant growth is not always demonstrated, especially under field conditions. In this study, we used a commercial inoculum on newly planted grapevines of a local cultivar grafted on a common rootstock R110. We followed the physiological status of vines, growth and productivity and functional biodiversity of soil bacteria during the first and second years of 20 inoculated with commercial inoculum bases on Rhizophagus irregularis and Funeliformis mosseaeAMF at field planting time and 20 non-inoculated control plants. All the parameters measured showed a neutral to negative effect on plant growth and production. The inoculated plants always presented lower values of photosynthesis, growth and grape production, although in some cases the differences did not reach statistical significance. On the contrary, the inoculation supposed an increase of the bacterial functional diversity, although the differences were not statistically significant either. Several studies show that the effect of inoculation with AMF is context-dependent. The non-favorable effects are probably due to inoculation ineffectiveness under complex field conditions and/or that, under certain conditions, AMF presence may be a parasitic association. This puts into question the effectiveness of its application in the field. Therefore, it is recommended to only resort to this type of biofertilizer when the cultivation conditions require it (e.g., very low previous microbial diversity, foreseeable stress due to drought, salinity, or lack of nutrients) and not as a general fertilization practice.

Drought effect on aromatic and phenolic potential of seven recovered grapevine varieties in Castilla-La Mancha region (Spain)

The effects of climate change are seriously affecting the quality of wine grapes. High temperatures and drought cause imbalances in the chemical composition of grapes. The result is overripe grapes with low acidity and high sugar content, which produce wines with excessive alcohol content, lacking in freshness and not very aromatic. As a consequence, the search of varieties with capacity of produce quality grapes in adverse climate conditions is a good alternative to preserve the sustainability of vineyards. In this work, quality parameters of seven Vitis vinifera L. cultivars (five whites and two reds) recently recovered from extinction and grown under two different hydric regimes (rainfed and irrigated) were analyzed during the 2020 vintage. At harvest time, weight of 100 berries, must physicochemical parameters (brix degree, total acidity, malic acid, pH), and carbon and oxygen isotope ratios (δ13C, δ18O) were determined. Subsequently, varietal aroma potential index (IPAv) and total polyphenol index (TPI) were analyzed. Quality parameters, IPAv and TPI, showed significant differences between varieties and water regimes. Both red varieties, Moribel and Tinto Fragoso, stood out for their high aromatic and phenolic potential, which was higher under rainfed regime. Regarding to white varieties, Montonera del Casar and Jarrosuelto stood out in terms of varietal aroma potential. Montonera del Casar high acidity in its musts and Jarrosuelto showed the highest berry weights.

Better understand the soil wet bulb formation with subsurface or aerial drip irrigation in viticulture

The gradual change in rainfall patterns experienced in the south of France vineyards, especially around the Mediterranean sea, means that the vines are increasingly subject to summer drought. The winegrowers developped the use of irrigation techniques to ensure the maintenance of competitive yields in the production of wines under Protected Geographical Indication label. In practice, drip irrigation pipes can be installed above the ground or buried into the soil as well as at different distances from the vine row. The objective of this study was to examine the profiles of the wet bulbs of the soil obtained from two drip irrigation systems : aerial drip located under the vine row and subsurface drip placed in the middle of the inter-row. This experiment took place over two consecutive seasons (2020-2021) on a 3.4 ha Viognier plot in the Mediterranean region (PGI Oc, France) on sandy clay soil. The annual rainfalls were less than 400 mm. Soil water content probes were installed at different depths (20 – 40 – 60 – 80 cm) and at different lateralities from the vine row (30 – 60 – 90 – 120 cm) to control the formation of the soil wet bulb during irrigation. The mapping and the analysis of the data allowed a better understanding and differentiation of the water percolation when irrigating with subsurface or aerial drip. For the same amount of water and without differences of vine water status, it is shown that in a subsurface drip irrigation situation, the size of the wet bulb formed is larger than in aerial drip irrigation system.

Traditional agroforestry vineyards, sources of inspiration for the agroecological transition of viticulture

A unique “terroir” can be found in southern Bolivia, which combines the specific features of climate, topography and altitude of high valleys, with the management of grapevines staked on trees. It is one of the rare remnants of agroforestry viticulture. A survey was carried out among 29 grapegrowers in three valleys, to characterize the structure and management of these vineyards, and identify the services they expect from trees. Farms were small (2.2 ha on average) and 85% of vineyards were less than 1 ha. Viticulture was associated with vegetable, fruit and fodder production, sometimes in the same fields. Molle trees were found in all plots, together with one or two other native tree species. Traditional grapevine varieties such as Negra Criolla, Moscatel de Alejandría and Vicchoqueña were grown with a large range of densities from 1550 to 9500 vines ha-1. From 18 to 30% of them were staked on trees, with 1.2 to 4.9 vines per tree. The management of these vineyards (irrigation, fertilization and grapevine protection) was described, the most particular technical operation being the coordinated pruning of trees and grapevines. Three types of management could be identified in the three valleys. Grapegrowers had a clear idea of the ecosystem services they expected from trees in their vineyards. The main one was protection against climate hazards (hail, frost, flood). Then they expected benefits in terms of pest and disease control, improvement of soil fertility and resulting yield. At last, some producers claimed that tree-staking was quicker and cheaper than conventional trellising. It can be hypothesized then that agroforestry is a promising technique for the agroecological transition of viticulture. Its contribution to the “terroir” of the high valleys of southern Bolivia and its link with the specificities of the wines and spirits produced there remain to be explored.