IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Quantification of Eugenol in various matrixes from hybrids vines. Case study of Armagnac white spirits production

Quantification of Eugenol in various matrixes from hybrids vines. Case study of Armagnac white spirits production

Abstract

Nowadays, winemaking is dealing with great challenges, notably climate change, disease resistance and low pesticide inputs, desire for more sustainable agricultural productions and permanent changing of consumer preference. Trying to propose practice improvements, scientists are exploring vine hybridization a paradoxically old but still promising way to take up such challenges (Pedneault & Provost, 2016). Phylloxera crisis in Europe (XIXth century) was a crucial step for improving hybridization in grapevine. Unfortunately some of the wines produced then presented redibitory sensorial default and were finally excluded for getting the PDO (Protected Designation of Origin) wine label in France.However, one grape variety from Armagnac vineyard was maintained despite the ban: the Baco blanc, a complex hybrid of Vitis labrusca x Vitis riparia x Vitis vinifera. Baco was created to be a disease tolerant and productive vine of white wine intended for distillation (Baco, 1925).Various matrixes, from vine shoots to distillates, are available for analysis in Armagnac wine spirit production. It was noticed that Baco blanc samples (all matrixes included) had an atypical chemical profile. Indeed, eugenol, a phenylpropenic compound, usually known to be extracted by contact with oak, is significantly more concentrated in white spirits made with Baco than same products made with V.vinifera cultivars. Eugenol was also quantified in other hybrids (Vitis riparia-containing hybrids as Marechal Foch and Frontenac) wines but in lower concentrations than in Baco blanc wines (Sun et al., 2011).Eugenol has a clove aroma and a noticeable and well-known antiseptic action, a gustative impact including an anesthesic power. These observations raise many questions, the main ones being: “Is there a link between tolerance of Baco to diseases and the presence of eugenol?”; “What is the dynamic of eugenol levels during spirit making?”; “Is there a link between the eugenol presence and the typicity of Baco wine spirits ?”Trying to answer these questions eugenol quantification was carried out by a HS-SPME-GC-MS method using deuterium labelled eugenol as internal standard and a procedure adapted to each matrix. An enzymatic hydrolysis (β-glucosidase enzyme) with addition of citrate-phosphate buffer was performed for plant material, musts and wines. The main goal was to highlight the existence of two eugenol fractions : a free one and a bound one. Such eugenol « cartography » resulted in interesting observations. First a greater eugenol concentration and accumulation during maturation occurs in Baco blanc than in other V.vinifera cultivars tested (Ugni blanc and Folle blanche). Second using enzymes increases the eugenol content during first steps of winemaking. Third, eugenol amounts seem to increase along with the storage duration on lees (before distillation). Finally, alambic characteristics may influence the alcohol content which may also impact eugenol concentration.

References

Baco, F. (1925). Précis complet de viticulture moderne et de vinification : Mes meilleures vignes hybrides franco-americaines : leurs principaux caractères : les meilleurs moyens pour les multiplier, les planter, les tailler, les cultiver, les vinifier (Imprimeries Gounouilhou)
Pedneault, K., & Provost, C. (2016). Fungus resistant grape varieties as a suitable alternative for organic wine production : Benefits, limits, and challenges. Scientia Horticulturae, 208, 57-77. https://doi.org/10.1016/j.scienta.2016.03.016
Sun, Q., Gates, M. J., Lavin, E. H., Acree, T. E., & Sacks, G. L. (2011). Comparison of Odor-Active Compounds in Grapes and Wines from Vitis vinifera and Non-Foxy American Grape Species. Journal of Agricultural and Food Chemistry, 59(19), 10657-10664. https://doi.org/10.1021/jf2026204

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Hastoy Xavier1, Franc Céline1, Riquier Laurent1, Marchand-Marion Stéphanie1, Ségur Marie-Claude2, Fermaud Marc3 and De Revel Gilles1

1Université de Bordeaux, INRAE, Bordeaux INP, OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
2Bureau National Interprofessionnel de l’Armagnac (BNIA), 32800 Eauze, France
3INRAE, UMR SAVE, UMTSeven, Bordeaux Science Agro, ISVV, F-33882, Villenave d’Ornon, France 

Contact the author

Keywords

Phenylpropenes, Baco blanc, Hybrid vines, White wine spirits, Armagnac

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Impact of climate variability and change on grape yield in Italy

Viticulture is entangled with weather and climate. Therefore, areas currently suitable for grape production can be challenged by climate change. Winegrowers in Italy already experiences the effect of climate change, especially in the form of warmer growing season, more frequent drought periods, and increased frequency of weather extremes.
The aim of this study is to investigate the impact of climate variability and change on grape yield in Italy to provide winegrowers the information needed to make their business more sustainable and resilient to climate change. We computed a specific range of bioclimatic indices, selected by the International Organisation of Vine and Wine (OIV), and correlated them to grape yield data. We have worked in collaboration with some wine consortiums in northern and central Italy, which provided grape yield data for our analysis.
Using climate variables from the E-OBS dataset we investigate how the bioclimatic indices changed in the past, and the impact of this change on grape productivity in the study areas. The climate impact on productivity is also investigated by using high-resolution convection-permitting models (CPMs – 2.2 horizontal resolution), with the purpose of estimating productivity in future emission scenarios. The CPMs are likely the best available option for this kind of impact studies since they allow a better representation of small-scale processes and features, explicitly resolve deep convection, and show an improved representation of extremes. In our study, we also compare CPMs with regional climate models (RCMs – 12 km horizontal resolution) to assess the added value of high-resolution models for impact studies. Further development of our study will lead to assessing the future suitability for vine cultivation and could lead to the construction of a statistical model for future projection of grape yield.

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

Analysis of some environmental factors and cultural practices that affect the production and quality of the Manto Negro, Callet and Prensal Blanc varieties

45 non irrigated vineyards distributed in the DO (Denomination) Pla i Llevant de Mallorca and the DO Binissalem Mallorca were used to investigate the characteristics of production and quality and their relationships certain environmental factors and cultural practices. The grape varieties investigated are autochthonous to the island of Mallorca, Manto Negro and Callet as red and Prensal Blanc as white. All plants were measured for four consecutive years in the main production and quality parameters. Among the environmental factors, the type of soil has been studied, more specifically its water retention capacity, the planting density, the age of the vineyard and the level of viral infection. The presence or absence of virus seems to have no effect on any component studied in the varieties studied. For the white variety Prensal Blanc age is negatively correlated with production and the number of bunches, nevertheless it does not cause any effect on the required quality parameters. However, for the red varieties Callet and Manto Negro, the age of the plantation is the variable that best correlates with the quality parameters, therefore the old vines should be the object of preservation by the viticulturists and winemakers in order to guarantee its contribution to the quality of the wines made with these varieties.

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.

Evolution of the amino acids content through grape ripening: Effect of foliar application of methyl jasmonate with or without urea

The parameters that determine the grape quality, and therefore the optimal harvest time, suffer variations during berry ripening, related to climate change, with the widely known problem of the gap between technological and phenolic maturities. However, there are few studies about its incidence on grape nitrogen composition. For this reason, the use of an elicitor, methyl jasmonate (MeJ), alone or with urea, is proposed as a tool to reduce climatic decoupling, allowing to establish the harvest time in order to achieve the optimum grape quality. The aim was to study the effect of MeJ and MeJ+Urea foliar applications on the evolution of Tempranillo amino acids content throughout the grape maturation. Three treatments were foliarly applied, at veraison and 7 days later: control (water), MeJ (10 mM) and MeJ+Urea (10 mM+6 kg N/ha). Grape samples were taken at five stages of maturation: day before the first and second applications, 15 days after the second application (pre-harvest), harvest day, and 15 days after harvest (post-harvest). The amino acids analysis of the samples was carried out by HPLC. Results showed that the evolution of amino acids was similar regardless of the treatment; however, foliar applications influenced the nitrogen compounds content, i.e., there was no qualitative effect but quantitative one. Most of the amino acids reached their maximum concentration in pre-harvest, being higher in grapes from the treatments than in the control. In general, no differences in grape amino acids content were observed between MeJ and MeJ+Urea treatments. Foliar applications with MeJ and MeJ+Urea enhanced the grape amino acids content, without affecting their profile, helping to optimize their quality and allowing to establish a more complete grape ripening standard. Therefore, MeJ and MeJ+Urea foliar applications can be a simple agronomic practice, which has shown promising results in order to enhance the grape quality.