IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Effect Of Grape Polysaccharides On The Volatile Composition Of Red Wines

Effect Of Grape Polysaccharides On The Volatile Composition Of Red Wines

Abstract

Yeast mannoproteins and derivates are polysaccharides produced from the cell walls of different yeast strains widely used in the winemaking and finning of wines to improve their overall stability and sensory properties. Some studies reported that mannoproteins maintain the wine aroma tending to be more appealing. On the contrary, grape polysaccharides are not commercially available, and the recovery of these compounds from grape by-products is nowadays a great challenge for the oenological research. These polysaccharides have a great potential in organoleptic finning since they have been reported to modulate the wine quality, as arabinogalactans which interacts with wine aroma compounds and increase their volatility (Ribeiro et al., 2014; Rinaldi et al., 2021).
In this study grape polysaccharide extracts obtained from different sources were used as finning agents at bottling in three wines from Vitis vinifera L. cv. Tempranillo and Graciano. Their effect on the volatile composition and profile was analyzed. Polysaccharides extracts were obtained from white pomace by-products (WP), red pomace by-products (RP), white must (WM), red must (RM), red wine (RW), and lees recovered after the winemaking (RL). Two more extracts with higher purification degrees were used (PE1 and PE2). The results were compared with a control (C) wine sample and with mannoproteins commercially available (CM).
The analysis of volatile compounds was performed using a GC-MS after a liquid-liquid extraction as described by Oliveira et al., 2006. Discriminant analyses were performed to differentiate the red wines by the fining extract used. WM, RM and CM wines were characterized by high contents of alcohols, C6 alcohols, some esters as ethyl isovalerate, acetates, acids, and terpenes. On the other hand, RW, RP, and RL wines were characterized by high contents of ethyl esters as ethyl lactate, ethyl hexanoate and ethyl octanoate, and volatile phenols, specially 4-vinylguaiacol and 4-ethylguaiacol. The wines treated with PE1 and PE2 were those which presented the lowest concentrations on most of the volatile compounds detected. Discriminant analyses showed that the use of the polysaccharide extracts modified the volatile composition of the wines.

Acknowledgements:

The authors would like to thank the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) for the funding provided for this study through the project RTA2017-00005-C02-02.

References

Oliveira, J. M., Faria, M., Sá, F., Barros, F., & Araújo, I. M. (2006). C6-alcohols as varietal markers for assessment of wine origin. Analytica Chimica Acta, 563(1-2 SPEC. ISS.), 300–309. https://doi.org/10.1016/j.aca.2005.12.029
Rinaldi, A., Gonzalez, A., Moio, L., & Gambuti, A. (2021). Commercial mannoproteins improve the mouthfeel and colour of wines obtained by excessive tannin extraction. Molecules, 26(14). https://doi.org/10.3390/molecules26144133
Ribeiro, T., Fernandes, C., Nunes, F. M., Filipe-Ribeiro, L., & Cosme, F. (2014). Influence of the structural features of commercial mannoproteins in white wine protein stabilization and chemical and sensory properties. Food Chemistry, 159, 47–54. https://doi.org/10.1016/j.foodchem.2014.02.149

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Curiel-Fernández María1,2, Canalejo Diego1,2, Zhao Feng1,2, Martínez-Lapuente Leticia1,2, Ayestarán Belén1,2, Cano-Mozo Estela1,2, Pérez-Magariño Silvia1,2, Guadalupe Zenaida1,2

1Instituto Tecnológico Agrario de Castilla y León
2Consejería de Agricultura y Ganadería 

Contact the author

Keywords

By-product valorization, grape pomace, lees, organoleptic modulation, grape polysaccharides

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Influence of agronomic practices in soil water content in mid-mountain vineyards

In the context of LIFE project MIDMACC (LIFE18 CCA/ES/001099), several pilots have been installed in vineyards in mid mountain areas of Catalonia (NE Spain) to test well stablished agronomic practices to increase the adaptation of Mediterranean mid mountain to climate change. Soil water content (SWC) at three different depths (15, 30 and 45cm) was measured in continuum from August 2020. One pilot (WC) included a well-established green cover (GC), a new GC (NC) and a conventional soil management (CM, tilling+herbicides). NC presented an intermediate state between WC and CM, responding similarly to CM in autumn but quickly reaching similar SWC to WC, then following the same evolution till next spring, with CM presenting lower values along autumn and winter. Then vegetation activation decreased SWC in all plots, (much slower in CM, lacking GC). Sensibility to spring rains is again intermediate for NC, which joins SWC evolution of CM by the end of spring till next autumn. It is expected that NC will resemble WC more and more as its GC develops. In the pilot combining vine training (VSP vs Gobelet) and hillside management (slope vs terrace), no clear pattern could be related with these conditions. However, both terraces seem to be more sensitive to spring rains. A third pilot included new vineyards (7 and 1 year old). In the new vineyard (N), higher canopy development, a spontaneous green cover and row straw resulted in a slower SWC dynamic, not so sensitive to rains but conserving more soil water in spring and most of summer, even with presumably a higher water extraction by vines. In the newest vineyard (VN) the deepest sensor is still sensitive to rain events all over the year and SWC is always highest at this depth, revealing small water capture by vines.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…

Elucidating vineyard site contributions to key sensory molecules: Identification of correlations between elemental composition and volatile aroma profile of site-specific Pinot noir wines

The reproducibility of elemental profile in wines produced across multiple vintages has been previously reported using grapes from a single scion clone of Vitis vinifera L. cv. Pinot noir. The grapevines were grown on fourteen different vineyard sites, from Oregon to southern California in the U.S.A., which span distances from approximately hundreds of meters to 1450 km, while elevations range from near sea level to nearly 500 m. In addition, sensorial (i.e. aroma, taste, and mouthfeel) and chemical (i.e. polyphenolic and volatile) differences across the different vineyard sites have also been observed among these wines at two aging time points. While strong evidence exists to support that grapes grown in different regions can produce wines with unique chemical and sensorial profiles, even when a single clone is used, the understanding of growing site characteristics that result in this reproducible differentiation continues to emerge. One hypothesis is that the elemental profile that a vineyard site imparts to the grape berries and the resulting wine is an important contributor to this differentiation in chemistry and sensory of wines. For example, various classes of enzymes that catalyze the formation of key aroma compounds or their precursors require specific metals. In this work, we begin to report correlations between elemental and volatile aroma profiles of site-specific Pinot noir wines, made under standardized winemaking conditions, that have been previously shown to be distinguished separately by these chemical analyses.

Inhibition of Oenococcus oeni during alcoholic fermentation by a selected Lactiplantibacillus plantarum strain

The use of selected cultures of the species Lactiplantibacillus plantarum in Oenology has grown in prominence in recent years. While initial applications of this species centred very much around malolactic fermentation (MLF), there is strong evidence to show that certain strains can be harnessed for their bio-protective effects. Unwanted spontaneous MLF during alcoholic fermentation (AF), driven by rogue Oenococcus oeni, is a winemaking deviation that is very difficult to manage when it occurs. This work set out to determine the efficacy of one particular strain of Lactiplantibacillus plantarum(Viniflora® NoVA™ Protect), against this problem in Cabernet Sauvignon must. The work was carried out at commercial scale and in a winery environment and compared the bio-protective culture with the more traditional approach of reducing must pH by the addition of tartaric acid. The combination of both was also investigated. The concentration of both Oenococcus oeni and Lactiplantibacillus plantarum was determined using qPCR. The adventitious Oenococcus oeni showed the most growth during AF in the control wine, whereas in the wines treated with Lactiplantibacillus plantarum a bacteriostatic effect against this species was observed. This effect was comparable to the wines treated with tartaric acid. This has particular commercial relevance for controlling the flora in musts with high pH, or when the addition of tartaric acid is either not permitted or is prohibitive for other reasons.

Amino nitrogen content in grapes: the impact of crop limitation

As an essential element for grapevine development and yield, nitrogen is also involved in the winemaking process and largely affects wine composition. Grape must amino nitrogen deficiency affects the alcoholic fermentation kinetics and alters the development of wine aroma precursors. It is therefore essential to control and optimize nitrogen use efficiency by the plant to guarantee suitable grape nitrogen composition at harvest. Understanding the impact of environmental conditions and cultural practices on the plant nitrogen metabolism would allow us to better orientate our technical choices with the objective of quality and sustainability (less inputs, higher efficiency). This trial focuses on the impact of crop limitation – that is a common practice in European viticulture – on nitrogen distribution in the plant and particularly on grape nitrogen composition. A wide gradient of crop load was set up in a homogeneous plot of Chasselas (Vitis vinifera) in the experimental vineyard of Agroscope, Switzerland. Dry weight and nitrogen dynamics were monitored in the roots, trunk, canopy and grapes, during two consecutive years, using a 15N-labeling method. Grape amino nitrogen content was assessed in both years, at veraison and at harvest. The close relationship between fruits and roots in the maintenance of plant nitrogen balance was highlighted. Interestingly, grape nitrogen concentration remained unchanged regardless of crop load to the detriment of the growth and nitrogen content of the roots. Meanwhile, the size and the nitrogen concentration of the canopy were not affected. Leaf gas exchange rates were reduced in response to lower yield conditions, reducing carbon and nitrogen assimilation and increasing intrinsic water use efficiency. The must amino nitrogen profiles could be discriminated as a function of crop load. These findings demonstrate the impact of plant balance on grape nitrogen composition and contribute to the improvement of predictive models and sustainable cultural practices in perennial crops.