IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Effect Of Grape Polysaccharides On The Volatile Composition Of Red Wines

Effect Of Grape Polysaccharides On The Volatile Composition Of Red Wines

Abstract

Yeast mannoproteins and derivates are polysaccharides produced from the cell walls of different yeast strains widely used in the winemaking and finning of wines to improve their overall stability and sensory properties. Some studies reported that mannoproteins maintain the wine aroma tending to be more appealing. On the contrary, grape polysaccharides are not commercially available, and the recovery of these compounds from grape by-products is nowadays a great challenge for the oenological research. These polysaccharides have a great potential in organoleptic finning since they have been reported to modulate the wine quality, as arabinogalactans which interacts with wine aroma compounds and increase their volatility (Ribeiro et al., 2014; Rinaldi et al., 2021).
In this study grape polysaccharide extracts obtained from different sources were used as finning agents at bottling in three wines from Vitis vinifera L. cv. Tempranillo and Graciano. Their effect on the volatile composition and profile was analyzed. Polysaccharides extracts were obtained from white pomace by-products (WP), red pomace by-products (RP), white must (WM), red must (RM), red wine (RW), and lees recovered after the winemaking (RL). Two more extracts with higher purification degrees were used (PE1 and PE2). The results were compared with a control (C) wine sample and with mannoproteins commercially available (CM).
The analysis of volatile compounds was performed using a GC-MS after a liquid-liquid extraction as described by Oliveira et al., 2006. Discriminant analyses were performed to differentiate the red wines by the fining extract used. WM, RM and CM wines were characterized by high contents of alcohols, C6 alcohols, some esters as ethyl isovalerate, acetates, acids, and terpenes. On the other hand, RW, RP, and RL wines were characterized by high contents of ethyl esters as ethyl lactate, ethyl hexanoate and ethyl octanoate, and volatile phenols, specially 4-vinylguaiacol and 4-ethylguaiacol. The wines treated with PE1 and PE2 were those which presented the lowest concentrations on most of the volatile compounds detected. Discriminant analyses showed that the use of the polysaccharide extracts modified the volatile composition of the wines.

Acknowledgements:

The authors would like to thank the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) for the funding provided for this study through the project RTA2017-00005-C02-02.

References

Oliveira, J. M., Faria, M., Sá, F., Barros, F., & Araújo, I. M. (2006). C6-alcohols as varietal markers for assessment of wine origin. Analytica Chimica Acta, 563(1-2 SPEC. ISS.), 300–309. https://doi.org/10.1016/j.aca.2005.12.029
Rinaldi, A., Gonzalez, A., Moio, L., & Gambuti, A. (2021). Commercial mannoproteins improve the mouthfeel and colour of wines obtained by excessive tannin extraction. Molecules, 26(14). https://doi.org/10.3390/molecules26144133
Ribeiro, T., Fernandes, C., Nunes, F. M., Filipe-Ribeiro, L., & Cosme, F. (2014). Influence of the structural features of commercial mannoproteins in white wine protein stabilization and chemical and sensory properties. Food Chemistry, 159, 47–54. https://doi.org/10.1016/j.foodchem.2014.02.149

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Curiel-Fernández María1,2, Canalejo Diego1,2, Zhao Feng1,2, Martínez-Lapuente Leticia1,2, Ayestarán Belén1,2, Cano-Mozo Estela1,2, Pérez-Magariño Silvia1,2, Guadalupe Zenaida1,2

1Instituto Tecnológico Agrario de Castilla y León
2Consejería de Agricultura y Ganadería 

Contact the author

Keywords

By-product valorization, grape pomace, lees, organoleptic modulation, grape polysaccharides

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Effect of vigour and number of clusters on eonological parameters and metabolic profile of Cabernet Sauvignon red wines

Vegetative growth and yield are reported to affect grape and wine quality. They can be controlled through different techniques linked to vine management. The objective of this research was to determine the effect of vine vigour and number of clusters per vine on physicochemical composition and phenolic profile of red wines. The experiment was carried out during two vegetative cycles, with cv. Cabernet Sauvignon grafted onto Paulsen 1103. Three vine vigour were defined, according to shoot weight at previous harvests, being low, medium and high. Five treatments of number of clusters were used for each vigour, with 15, 22, 29, 36, and 45 clusters per vine. Grapes from all treatments were harvested in the same day from Brix and total acidity criteria. Thirty days after bottling, classical analyzes and phenolic compounds were performed. As results, different responses were obtained from each vintage. In 2020, a dry season from veraison to harvest, grapes and wines obtained from low vigour treatment and 45 clusters per vine was the highest in sugar and alcohol content respectively, while grapes and wines from high vigour and 15 clusters presented the lowest sugar and alcohol content. Total anthocyanins were higher in treatment with low vigour and 15 clusters, while the lowest amounts were found in low vigour with 45 clusters, as well as medium and high vigour with 36 clusters per vine. Total tannins were higher in high vigour with 22 clusters and medium vigour with 29 clusters, while were lower in low vigour with 36 clusters. In 2021, a wet season at harvest, responses were different, and great variations were observed between treatments. As conclusions, yield and vine vigour had strong influence on grape and wine quality, promoting different enological potentials on which can be indicated/used for aging strategies of red and even rosé wines.

Mobile device to induce heat-stress on grapevine berries

Studying heat stress response of grapevine berries in the field often relies on weather conditions during the growing season. We constructed a mobile heating device, able to induce controlled heat stress on grapes in vineyards. The heater consisted of six 150 W infrared lamps mounted in a profile frame. Heating power of the lamps could be controlled individually by a control unit consisting of a single board computer and six temperature sensors to reach a pre-set temperature. The heat energy applied to individual berries within a cluster decreases by the squared distance to the heat source, enabling the establishment of temperature profiles within individual clusters. These profiles can be measured by infrared thermography once a steady state has been reached. Radiant flux density received by a berry depending on the distance was calculated based on a view factor and measured lamp surface temperature and resulted to 665 Wm-2 at 7cm. Infrared thermography of the fruit surface was in good agreement with measurements conducted with a thermocouple inserted at epidermis level. In combination with infrared thermography, the presented device offers possibilities for a wide range of applications like phenotyping for heat tolerance in the field to proceed in the understanding of the complex response of plants to heat stress. Sunburn necrosis symptoms were artificially induced with the aid of the device for cv. Bacchus and cv. Sylvaner in the 2020 and 2021 growing season. Threshold temperatures for sunburn induction (LT5030min) were derived from temperature data of single berries and visual sunburn assessment, applying logistic regression. A comparison of threshold temperatures for the occurrence of sunburn necrosis confirmed the higher susceptibility of cv. Bacchus. The lower susceptibility of cv. Sylvaner did not seem to be related to its phenolic composition, rendering a thermoprotective role of berry phenolic compounds unlikely.

Aromatic maturity is a cornerstone of terroir expression in red wine

Harvesting grapes at adequate maturity is key to the production of high-quality red wines. Enologists and wine makers define several types of maturity, including technical maturity, phenolic maturity and aromatic maturity. Technical maturity and phenolic maturity are relatively well documented in the scientific literature, while articles on aromatic maturity are scarcer. This is surprising, because aromatic maturity is, without a doubt, the most important of the three in determining wine quality and typicity (including terroir expression). Optimal terroir expression can be obtained when the different types of maturity are reached at the same time, or within a short time frame. This is more likely to occur when the ripening takes place under mild temperatures, neither too cool, nor too hot. Aromatic expression in wine can be driven, from low to high maturity, by green, herbal, fresh fruit, ripe fruit, jammy fruit, candied fruit or cooked fruit aromas. Green and cooked fruit aromas are not desirable in red wines, while the levels of other aromatic compounds contribute to the typicity of the wine in relation to its origin. Wines produced in cool climates, or on cool soils in temperate climates, are likely to express herbal or fresh fruit aromas; while wines produced under warm climates, or on warm soils in temperate climates, may express ripe fruit, jammy fruit or candied fruit aromas. Growers can optimize terroir expression through their choice of grapevine variety. Early ripening varieties perform better in cool climates and late ripening varieties in warm climates. Additionally, maturity can be advanced or delayed by different canopy management practices or training systems.

Sustaining wine identity through intra-varietal diversification

With contemporary climate change, cultivated Vitis vinifera L. is at risk as climate is a critical component in defining ecologically fitted plant materiel. While winegrowers can draw on the rich diversity among grapevine varieties to limit expected impacts (Morales-Castilla et al., 2020), replacing a signature variety that has created a sense of local distinctiveness may lead to several challenges. In order to sustain wine identity in uncertain climate outcomes, the study of intra-varietal diversity is important to reflect the adaptive and evolutionary potential of current cultivated varieties. The aim of this ongoing study is to understand to what extent can intra-varietal diversity be a climate change adaptation solution. With a focus on early (Sauvignon blanc, Riesling, Grolleau, Pinot noir) to moderate late (Chenin, Petit Verdot, Cabernet franc) ripening varieties, data was collected for flowering and veraison for the various studied accessions (from conservatory plots) and clones. For these phenological growing stages, heat requirements were established using nearby weather stations (adapted from the GFV model, Parker et al., 2013) and model performances were verified. Climate change projections were then integrated to predict the future behaviour of the intra-varietal diversity. Study findings highlight the strong phenotypic diversity of studied varieties and the importance of diversification to enhance climate change resilience. While model performances may require improvements, this study is the first step towards quantifying heat requirements of different clones and how they can provide adaptation solutions for winegrowers to sustain local wine identity in a global changing climate. As genetic diversity is an ongoing process through point mutations and epigenetic adaptations, perspective work is to explore clonal data from a wide variety of geographic locations.

Exploring resilience and competitiveness of wine estates in Languedoc-Roussillon in the recent past: a multi-level perspective

The Languedoc-Roussillon wineries are facing a decline in wine yields particularly PGI yields due to many factors. Climate change is just ones, but is expected to increase in the future. There is also structurally a large heterogeneity of yield profiles among terroirs, varieties and strategies. This work investigates the link between yield, competitiveness and resilience to explore how resilient winegrowers have been in the recent past. To this end two approaches have been combined; (i) an accountancy database analysis at estate scale and (ii) municipality level competitiveness analysis. A new resilience indicator that characterizes the capacity of an estate to absorb yield variation is also defined. The FADN database between 2000 and 2018 of ex-Languedoc-Roussillon (France) and other data are used to analyse the current situation and the past evolution of competitiveness and resilience by type of estate (type of farm: PGI and/or PDO & type of commercialization: bulk and/or bottles). The net margin, which defines competitiveness, is not correlated to yield for all types but depends on the type of commercialization and the level of specialisation. The resilience indicator shows that the net margin of estates specialized in PGI is particularly sensitive to yield declines. We also show that price evolutions seem to compensate the effect of yield losses for the majority of types. Municipality scale analysis shows the links between local pedoclimate, yield, commercialization strategies and price. Overlapping a PDO with a PGI does not always increase a municipality’s PGI competitiveness. It is difficult to make links between causes and effects due to the complexity of the wine production system. Production diversification may be a solution. Resorting to the two level of analysis helps resolving the data gap that is necessary to explore the links between yield and economic performance of the wine estates in the long term.