IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Kinetic investigations of the Gewürztraminer volatile organic compounds and color at different temperatures and pHs

Kinetic investigations of the Gewürztraminer volatile organic compounds and color at different temperatures and pHs

Abstract

Gewürztraminer is a well-known wine famous for its aroma profile, which is characterized by rose petals, cloves, lychees, and other tropical fruit notes. It is cultivated worldwide, including the Trentino Alto Adige region located in northern Italy, especially in the Tramin zone, and it has long been studied trying to understand what the most characterizing volatile aroma components are [1-4]. The terpenes (geraniol, cis rose oxide, citronellol, and linalool) are between the major responsible for the characteristic floral aroma of this cultivar’s grapes and wines. Throughout the winemaking and storage, acid-catalysed rearrangements take place producing cyclic and hydroxylated forms of the above terpenes, which generally have minor perception thresholds and so the wine’s floral aroma character decreases [5]. It has been demonstrated that the temperature and pH strongly influence these reactions, however their kinetics are not studied in detail.

The first aim of this work was to develop and validate a fast, modern, sensitive, selective, robust, and comprehensive protocol for the quantification of primary, secondary, and tertiary wine volatile compounds by using solid-phase extraction (SPE) cartridges for the sample preparation and a fast GC-MS/MS for analysis [1]. Second aim was to apply this protocol and study the kinetics of the reactions occurring on the Gewürztraminer wine volatile compounds during its storage at various temperatures and pHs. In parallel also
the colour of the wines was monitored by using the CIELAB method. The produced method gave us the possibility to measure 64 aroma compounds, with big importance in wine science, by using fewer organic solvents, having short chromatographic run, and increasing specificity and sensitivity due to the MRM MS-mode used.

The results of the second part of the study, demonstrated the behaviour of volatile aroma compounds, with their absolute concentrations. The investigated reactions included the degradation of the linear terpenes (linalool, geraniol, nerol, etc), the ethyl esters of fatty
acids and volatile phenols on the one hand; and the formation of the cyclic terpenes (1,4-cineole, 1,8-cineole, terpineol, etc), the norisoprenoids (e.g. TDN and safranal) and the diprotic organic acids esters on the other hand.

In conclusion, we developed a modern protocol for the analysis of the wine aroma compounds and we underlined some key characteristics that a winemaker should take in consideration in the Gewürztraminer production and aging/storage. 

References

1. Carlin, S.; Lotti, C.; Correggi, L.; Mattivi, F.; Arapitsas, P.; Vrhovsek, U. “Measurement of the effect of accelerated aging on the aromatic compounds of Gewürztraminer and Teroldego wines, using a new SPE-GC-MS /MS protocol” Metabolites 2022, 12(2), 180.
2. Versini, G. Sull’aroma Del Vino “Traminer Aromatico” o “Gewürztraminer.” VIGNEVINI 1985, 12, 57–65.
3. Guth, H. Identification of Character Impact Odorants of Different White Wine Varieties. J. Agric. Food Chem. 1997, 45, 3022–3026. 
4. Román, T.; Tonidandel, T.; Larcher, R.; Celotti, E.; Nicolini, G. Importance of Polyfunctional Thiols on Semi-Industrial Gewürztraminer Wines and the Correlation to Technological Treatments. Eur. Food Res. Technol. 2018, 244, 379–386. 
5. Slaghenaufi, D.; Ugliano, M. “Norisoprenoids, Sesquiterpenes and Terpenoids Content of Valpolicella Wines During Aging: Investigating Aroma Potential in Relationship to Evolution of Tobacco and Balsamic Aroma in Aged Wine.” Front. Chem. 2018, 6.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Carlin Silvia1, Lotti Cesare1, Rapaccioli Attilio1, Mattivi, Fulvio1,2, Trenti Gianmaria3, Vrhovsek Urska1 and Arapitsas Panagiotis1,4

1Metabolomics Unit, Research and Innovation Centre Edmund Mach Foundation, Via Edmund Mach 1, 38010 San Michele all’ Adige, Italy
2Department of Cellular Computational and Integrative Biology, University of Trento, Via Sommarive 9, 38123 Povo, Trento, Italy
3Winery, Edmund Mach Foundation, Via Edmund Mach 1, 38010 San Michele all’Adige, Italy
4Department of Wine, Vine and Beverage Sciences, School of Food Science, University of West Attica, Ag. Spyridonos str, Egaleo, 12243 Athens, Greece.

Contact the author

Keywords

fastGC, accelerated aging, storage, terpenes, Cielab

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Deconstructing the soil component of terroir: from controversy to consensus

Wine terroir describes the collectively recognized relation between a geographical area and the distinctive organoleptic characteristics of the wines produced in it. The overriding objective in terroir studies is therefore to provide scientific proof relating the properties of terroir components to wine quality and typicity. In scientific circles, the role of climate (macro-, meso- and micro-) on grape and wine characteristics is well documented and accepted as the most critical. Moreover, there has been increasing interest in recent years about new elements with possible importance in shaping wine terroir like berry/leaf/soil microbiology or even aromatic plants in proximity to the vineyard conferring flavors to the grapes. However, the actual effect of these factors is also dependent on complex interactions with plant material (variety/clone, rootstock, vine age) and with human factors.
The contribution of soil, although a fundamental component of terroir and extremely popular among wine enthusiasts, remains a much-debated issue among researchers. The role of geology is probably the one mostly associated by consumers with the notion of terroir with different parent rocks considered to give birth to different wine styles. However, the relationship between wine properties and the underlying parent material raises a lot of controversy especially regarding the actual existence of rock-derived flavors in the wine (e.g. minerality). As far as the actual soil properties are concerned, the effect of soil physical properties is generally regarded as the most significant (e.g sandy soils being associated with lighter wines while those on clay with colored and tannic ones) mostly through control of water availability which ultimately modifies berry ripening conditions either directly by triggering biosynthetic pathways, or indirectly by altering vigor and yield components. The role of soil chemistry seems to be weakly associated to wine sensory characteristic, although N, K, S and Ca, but also soil pH, are often considered important in the overall soil effect.
Recently, in the light of evidence provided by precision agriculture studies reporting a high variability of vineyard soils, the spatial scale should also be taken into consideration in the evaluation of the soil effects on wines. While it is accepted that soil effects become more significant than climate on a local level, it is not clear whether these micro-variations of vineyard soils are determining in the terroir effect. Moreover, as terroir is not a set of only natural factors, the magnitude of the contribution of human-related factors (irrigation, fertilization, soil management) to the soil effect still remains ambiguous. Lastly, a major shortcoming of the majority of works about soil effects on wine characteristics is the absence of connection with actual vine physiological processes since all soil effects on grape and wine chemistry and sensorial properties are ultimately mediated through vine responses.
This article attempts to breakdown the main soil attributes involved in the terroir effect to suggest an improved understanding about soil’s true contribution to wine sensory characteristics. It is proposed that soil parameters per se are not as significant determining factors in the terroir effect but rather their mutual interactions as well as with other natural and human factors included in the terroir concept. Consequently, similarly to bioclimatic indices, composite soil indices (i.e. soil depth, water holding capacity, fertility, temperature etc), incorporating multiple soil parameters, might provide a more accurate and quantifiable means to assess the relative weight of the soil component in the terroir effect.

Terroir analysis and its complexity

Terroir is not only a geographical site, but it is a more complex concept able to express the “collective knowledge of the interactions” between the environment and the vines mediated through human action and “providing distinctive characteristics” to the final product (OIV 2010). It is often treated and accepted as a “black box”, in which the relationships between wine and its origin have not been clearly explained. Nevertheless, it is well known that terroir expression is strongly dependent on the physical environment, and in particular on the interaction between soil-plant and atmosphere system, which influences the grapevine responses, grapes composition and wine quality. The Terroir studying and mapping are based on viticultural zoning procedures, obtained with different levels of know-how, at different spatial and temporal scales, empiricism and complexity in the description of involved bio-physical processes, and integrating or not the multidisciplinary nature of the terroir. The scientific understanding of the mechanisms ruling both the vineyard variability and the quality of grapes is one of the most important scientific focuses of terroir research. In fact, this know-how is crucial for supporting the analysis of climate change impacts on terroir resilience, identifying new promised lands for viticulture, and driving vineyard management toward a target oenological goal. In this contribution, an overview of the last findings in terroir studies and approaches will be shown with special attention to the terroir resilience analysis to climate change, facing the use and abuse of terroir concept and new technology able to support it and identifying the terroir zones.

austrianvineyards.com: online viewer of all designations of Austrian wine

To digitally record and present all the origins of Austrian wines in the same perfect and clear way was the motivation for the Austrian Wine Marketing Board (Austrian Wine) to start with the project in 2018. In June 2021 the results were presented to the public in an online viewer showing all the designations of Austrian wine, available at https://austrianvineyards.com in a largely barrier-free manner. The online viewer provides tailored individual maps fitted to the respective zoom level. The smallest unit of wine-origins in Austria is called Ried and is displayed in a plot-specific manner highlighting areas under vine. Information on the Ried include administrative district, winegrowing municipality, cadastral municipality, large collective vineyard site, specific winegrowing region, generic winegrowing region, winegrowing area and, in many cases, an illustrative picture. Complementary data on the size, elevation (minimum-maximum), orientation (in 8 sectors plus flat) and gradient (minimum, maximum, average) are based on the area under vine according to the EU’s Integrated Administration and Control System. Additional information covers climate data. The diagrams are taken from the monthly breakdown of data in the annals of the Central Institute for Meteorology and Geodynamics, Austria provide a display of values for air temperature, precipitation, and sunshine hours for the reference year and the long-term average. Seasonal aggregated data on temperature, precipitation, and sunshine hours complete the display. Short descriptions with emphasis on geology and soil, field name in historical maps, etymology of the denomination, and main planted variety complements the available information for the main designations in the online viewer. These descriptions are compiled by winegrowers, geologists, historians, and journalists. All the information and data can be extracted to a pdf-file. Printed vineyard maps are also available. Missing content regarding wine origins in Styria will be completed in winter 2021/22.

Second pruning as a strategy to delay maturation in cv. ‘Touriga nacional’ in the Portuguese Douro region

The advance in maturation of wine grapes is an important climate change risk related effect that could affect warm regions like Portuguese Douro Wine Region. Indeed, the climate analysis over the past years registered a decrease in the precipitation, significant higher average temperatures, and a more frequent occurrence of extreme weather events, including heat waves. In these conditions the length from anthesis until maturation is shortened and the uncoupling of technical and phenolic maturity results in berries with higher sugar concentration (and lower acidity), but lower anthocyanins, tannins, and total phenolic concentration, which produce unbalanced wines.
In this work, an innovative strategy of crop forcing, based on forcing vine regrowth after a second pruning of green shoots, was tested, aimed at delaying ripening until the temperature becomes lower and, therefore, preventing acidity loss and increasing anthocyanin-to-sugar ratio. The experiments were conducted in 2019 and 2020 in a commercial vineyard of ‘Touriga Nacional’ located in the Douro Region. Crop forcing was conducted 15 (CF1) to 30 (CF2) days after fruit set. Vines pruned with conventional methods were used as control (CF0). Results confirmed that fruit ripening was shifted from the hot season (August/September), until a cooler period (October through early-November). At harvest, grapevine berries from CF1 and CF2 presented lower pH and higher acidity, than control, with no significant differences in colour intensity and phenolic levels composition. Sugar content was lower in CF2-treated vines in both seasons. However, in CF-treated vines the number and size of clusters were significantly lower (up to 88% reduction) than in control plants. A metabolomics analysis of mature berries from CF-treated vines and control is underway. Crop forcing was indeed effective in producing a more balance berry composition but severely reduced grapevine yield,