IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Kinetic investigations of the Gewürztraminer volatile organic compounds and color at different temperatures and pHs

Kinetic investigations of the Gewürztraminer volatile organic compounds and color at different temperatures and pHs

Abstract

Gewürztraminer is a well-known wine famous for its aroma profile, which is characterized by rose petals, cloves, lychees, and other tropical fruit notes. It is cultivated worldwide, including the Trentino Alto Adige region located in northern Italy, especially in the Tramin zone, and it has long been studied trying to understand what the most characterizing volatile aroma components are [1-4]. The terpenes (geraniol, cis rose oxide, citronellol, and linalool) are between the major responsible for the characteristic floral aroma of this cultivar’s grapes and wines. Throughout the winemaking and storage, acid-catalysed rearrangements take place producing cyclic and hydroxylated forms of the above terpenes, which generally have minor perception thresholds and so the wine’s floral aroma character decreases [5]. It has been demonstrated that the temperature and pH strongly influence these reactions, however their kinetics are not studied in detail.

The first aim of this work was to develop and validate a fast, modern, sensitive, selective, robust, and comprehensive protocol for the quantification of primary, secondary, and tertiary wine volatile compounds by using solid-phase extraction (SPE) cartridges for the sample preparation and a fast GC-MS/MS for analysis [1]. Second aim was to apply this protocol and study the kinetics of the reactions occurring on the Gewürztraminer wine volatile compounds during its storage at various temperatures and pHs. In parallel also
the colour of the wines was monitored by using the CIELAB method. The produced method gave us the possibility to measure 64 aroma compounds, with big importance in wine science, by using fewer organic solvents, having short chromatographic run, and increasing specificity and sensitivity due to the MRM MS-mode used.

The results of the second part of the study, demonstrated the behaviour of volatile aroma compounds, with their absolute concentrations. The investigated reactions included the degradation of the linear terpenes (linalool, geraniol, nerol, etc), the ethyl esters of fatty
acids and volatile phenols on the one hand; and the formation of the cyclic terpenes (1,4-cineole, 1,8-cineole, terpineol, etc), the norisoprenoids (e.g. TDN and safranal) and the diprotic organic acids esters on the other hand.

In conclusion, we developed a modern protocol for the analysis of the wine aroma compounds and we underlined some key characteristics that a winemaker should take in consideration in the Gewürztraminer production and aging/storage. 

References

1. Carlin, S.; Lotti, C.; Correggi, L.; Mattivi, F.; Arapitsas, P.; Vrhovsek, U. “Measurement of the effect of accelerated aging on the aromatic compounds of Gewürztraminer and Teroldego wines, using a new SPE-GC-MS /MS protocol” Metabolites 2022, 12(2), 180.
2. Versini, G. Sull’aroma Del Vino “Traminer Aromatico” o “Gewürztraminer.” VIGNEVINI 1985, 12, 57–65.
3. Guth, H. Identification of Character Impact Odorants of Different White Wine Varieties. J. Agric. Food Chem. 1997, 45, 3022–3026. 
4. Román, T.; Tonidandel, T.; Larcher, R.; Celotti, E.; Nicolini, G. Importance of Polyfunctional Thiols on Semi-Industrial Gewürztraminer Wines and the Correlation to Technological Treatments. Eur. Food Res. Technol. 2018, 244, 379–386. 
5. Slaghenaufi, D.; Ugliano, M. “Norisoprenoids, Sesquiterpenes and Terpenoids Content of Valpolicella Wines During Aging: Investigating Aroma Potential in Relationship to Evolution of Tobacco and Balsamic Aroma in Aged Wine.” Front. Chem. 2018, 6.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Carlin Silvia1, Lotti Cesare1, Rapaccioli Attilio1, Mattivi, Fulvio1,2, Trenti Gianmaria3, Vrhovsek Urska1 and Arapitsas Panagiotis1,4

1Metabolomics Unit, Research and Innovation Centre Edmund Mach Foundation, Via Edmund Mach 1, 38010 San Michele all’ Adige, Italy
2Department of Cellular Computational and Integrative Biology, University of Trento, Via Sommarive 9, 38123 Povo, Trento, Italy
3Winery, Edmund Mach Foundation, Via Edmund Mach 1, 38010 San Michele all’Adige, Italy
4Department of Wine, Vine and Beverage Sciences, School of Food Science, University of West Attica, Ag. Spyridonos str, Egaleo, 12243 Athens, Greece.

Contact the author

Keywords

fastGC, accelerated aging, storage, terpenes, Cielab

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

The interplay between grape ripening and weather anomalies – A modeling exercise

Current climate change is increasing inter- and intra-annual variability in atmospheric conditions leading to grapevine phenological shifts as well altered grape ripening and composition at ripeness. This study aims to (i) detect weather anomalies within a long-term time series, (ii) model grape ripening revealing altered traits in time to target specific ripeness thresholds for four Vitis vinifera cultivars, and (iii) establish empirical relationships between ripening and weather anomalies with forecasting purposes. The Day of the Year (DOY) to reach specific grape ripeness targets was determined from time series of sugar concentrations, total acidity and pH collected from a private company in the period 2009-2021 in North-Eastern Italy. Non-linear models for the DOY to reach the specified ripeness thresholds were assessed for model efficiency (EF) and error of prediction (RMSE) in four grapevine cultivars (Merlot, Cabernet Sauvignon, Glera and Garganega). For each vintage and cultivar, advances or delays in DOY to target specified ripeness thresholds were assessed with respect to the average ripening dynamics. Long-term meteorological series monitored at ground weather station by means of hourly air temperature and rainfall data were analyzed. Climate statistics were obtained and for each time period (month, bimester, quarter and year) weather anomalies were identified. A linear regression analysis was performed to assess a possible correlation that may exist between ripening and weather anomalies. For each cultivar, ripeness advances or delays expressed in number of days to target the specific ripening threshold were assessed in relation to registered weather anomalies and the specific reference time period in the vintage. Precipitation of the warmest month and spring quarter are key to understanding the effect of climate change on sugar ripeness. Minimum temperatures of May-June bimester and maximum temperatures of spring quarter best correlate with altered total acidity evolution and pH increment during the ripening process, respectively.

Projected changes in vine phenology of two varieties with different thermal requirements cultivated in La Mancha DO (Spain) under climate change scenarios

The aim of this work was to analyze the phenology variability of Tempranillo and Chardonnay cultivars, related to the climatic characteristics in La Mancha Designation of Origin, and their potential changes under climate change scenarios. Phenological dates referred to budbreak, flowering, veraison and harvest were analyzed for the period 2000-2019. The weather conditions at daily time scale, recorded during the same period, were also evaluated. The thermal requirements to reach each of these phenological stages were calculated and expressed as the GDD accumulated from DOY=60. Changes in phenology were projected by 2050 and 2070 taking into account those values and the projected temperatures and precipitation, simulated under two Representative Concentration Pathway (RCP) scenarios –RCP4.5 and RCP8.5– using an ensemble of models. The average phenological dates during the period under study were, April 16th ± 6.6 days and April 5th ± 6.0 days for budbreak, May 31st ± 6.0 days and May 27th ± 5.3 days for flowering, July 26th ± 5.6 days and July 25th ± 5.8 days for veraison, and Ago 23rd ± 10.8 days and Ago 17th ± 9.0 days for harvest, respectively, for Tempranillo and Chardonnay. The projected changes in temperature imply an average change in the maximum growing season (April-August) temperatures of 1.2 and 1.9°C by 2050, and 1.6 and 2.6°C by 2070, under the RCP4.5 and RCP8.5 scenarios, respectively. A reduction in precipitation is predicted, which vary between 15% for 2050 under RCP4.5 scenario and up to 30% by 2070 under RCP8.5. The advance of the phenological dates for 2050, could be of 6, 7, 7, and 8 days for Tempranillo and 4, 6, 6 and 9 days for Chardonnay, respectively for budbreak, flowering, veraison and harvest under the RCP4.5 scenario. Under the RCP8.5 emission scenario, the advance could be up to 30% higher.

Effect of partial net shading on the temperature and radiation in the grapevine canopy, consequences on the grape quality of cv. Gros Manseng in PDO Pacherenc-du-vic-Bilh

As elsewhere, southwestern France vineyards face more recurrent summer heat waves these last years. Among the possibilities of adaptation to this climate changing parameter, the use of net shading is a technique that allow for limiting canopy exposure to radiations. In this trial, we tested net shading installed on one face of the canopy, on a north-south row-oriented plot of cv. Gros Manseng trained on VSP system in the PDO Pacherenc-du-Vic-Bilh. The purpose was to characterize the effects on the ambient canopy temperatures and radiations during the season and to observe the consequences on the composition of grapes and wines. Two sorts of net were used with two levels of obstruction (50% and 75%) of the photosynthesis active radiation (PAR). They have been installed on the west side of the canopy and compared to a netless control. Temperature and PAR sensors registered hourly data during the season. On specific summer day (hot and sunny) manual measurements took also place on bunches (temperature) and in different spots of the canopy (PAR). The results showed that, on clear days, the radiation is lowered by the shade nets respecting the supplier criteria. The effects on the ambient canopy temperature were inconstant on this plot when we observed the data from the global period of shading between fruit set and harvest. However, during hot days (>30°C), the temperature in the canopy was reduced during afternoon and the temperature of the bunch surface was reduced as well comparing to the control. A decrease of the maturity parameters of the berries, sugar and acidity, was also observed. Concerning the wine aromatic potential, no differences clearly appeared.

Investigating the impact of grape exposure and UV radiations on rotundone in Vitis vinifera L. Tardif grapes under field trial conditions

Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing sustainable viticultural strategies to enhance rotundone accumulation or limit its degradation. It was recently proposed that ultraviolet (UV) radiations could stimulate rotundone production. The aim of this study was to investigate under field trial conditions the impact of grape exposure and UV treatments on rotundone in Vitis vinifera L. Tardif, an almost extinct grape variety from south-west France that can express particularly high rotundone levels. Four different treatments were compared in 2021 to a control treatment using a randomised complete block design with three replications per treatment. Grape exposure was manipulated through early or late defoliation. Leaf and laterals shoots were removed at Eichorn Lorenz growth stages 32 or 34 on the morning-sun side of the canopy. During grape maturation, UV radiations were either reduced by 99% by installing UV radiation-shielding sheets, or applied four times using the Boxilumix™ non thermal device (Asclepios Tech, Tournefeuille) with the aim of activating plant signalling pathway. Loggers displayed in solar radiation shields were used to assess the effect of such shielding sheets on air temperature within the bunch zone. The composition of grapes subjected to these treatments will be soon analysed for their rotundone content and basic classical laboratory analyses. Grapes will be harvested to elaborate wines under standardized small-scale vinification conditions (60kg) that will be assessed by a trained sensory panel.

Better understand the soil wet bulb formation with subsurface or aerial drip irrigation in viticulture

The gradual change in rainfall patterns experienced in the south of France vineyards, especially around the Mediterranean sea, means that the vines are increasingly subject to summer drought. The winegrowers developped the use of irrigation techniques to ensure the maintenance of competitive yields in the production of wines under Protected Geographical Indication label. In practice, drip irrigation pipes can be installed above the ground or buried into the soil as well as at different distances from the vine row. The objective of this study was to examine the profiles of the wet bulbs of the soil obtained from two drip irrigation systems : aerial drip located under the vine row and subsurface drip placed in the middle of the inter-row. This experiment took place over two consecutive seasons (2020-2021) on a 3.4 ha Viognier plot in the Mediterranean region (PGI Oc, France) on sandy clay soil. The annual rainfalls were less than 400 mm. Soil water content probes were installed at different depths (20 – 40 – 60 – 80 cm) and at different lateralities from the vine row (30 – 60 – 90 – 120 cm) to control the formation of the soil wet bulb during irrigation. The mapping and the analysis of the data allowed a better understanding and differentiation of the water percolation when irrigating with subsurface or aerial drip. For the same amount of water and without differences of vine water status, it is shown that in a subsurface drip irrigation situation, the size of the wet bulb formed is larger than in aerial drip irrigation system.