IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Biochemical characterization of grape skin cell wall during ripening in relation to Botrytis cinerea susceptibility of two Champagne cultivars

Biochemical characterization of grape skin cell wall during ripening in relation to Botrytis cinerea susceptibility of two Champagne cultivars

Abstract

Pectins or pectic polysaccharides are one of the major components in grape skin cell wall, they contribute to physiological processes which determine the integrity and rigidity of grape skin tissue. Their composition and organization in the cell wall matrix differ according cultivars and also play an important role in the defense mechanisms against plant pathogen and wounding. During grape ripening, important structural and biochemical changes are modifying the cell wall integrity due to pectolytic enzymes such as pectin methylesterases and polygalacturonases which participate to the cell wall weakening and increase the grape susceptibility to pathogens such as Botrytis cinerea.This work investigated the distribution of pectic polysaccharides in the cell wall according to their molecular weight and the localization of pectins (homogalacturonans) highly and low methyl-esterified in grape skin tissue throughout the berry development of the two main Champagne cultivars (Vitis vinifera cv. Pinot noir and Chardonnay), in relation with in vitro Botrytis cinerea susceptibility tests. The skin cell wall composition was evaluated by size exclusion chromatography (SEC) and the pectin localization by immunogold labelling.The comparative study between the two main grape cultivars from Champagne region highlights differences in pectin composition, Chardonnay skins are characterized by less pectic polysaccharides of high molecular weight (HMW) related to a lower susceptibility to Botrytis cinerea. The pectins cellular localization showed that pectins highly methyl-esterified are more important in Pinot noir cell walls than Chardonnay ones, suggesting different mechanisms of cell walls degradation between Chardonnay and Pinot noir skins.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Andre Marie1, Lacampagne Soizic1, Barsacq Audrey1, Mercier Laurence2 and Gény-Denis Laurence1

1Unité mixte de recherche Œnologie, UMR 1366 Université de Bordeaux, INRAE, Bordeaux INP, ISVV MHCS, Epernay, 33882, Villenave d’Ornon, France
2MHCS, Epernay, France

Contact the author

Keywords

skin, ripening, pectins, SEC, Champagne

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Adaptation to climate change by determining grapevine cultivar differences using temperature-based phenology models

Grapevine phenology is advancing with increased temperatures associated with climate change. This may result in higher fruit sugar concentrations at harvest and/or earlier compressed harvests and changes in the synchrony of sugar with other fruit metabolites. One adaptation strategy that growers may use to maintain typicity of wine style is to change cultivars. This approach may enable fruit

SAVOIR: A project promoting innovative and effective prophylactic methods in viticulture, as part of the governmental plan to anticipate the withdrawal of plant protection products in France (PARSADA)

Faced with the likely withdrawal of commercial specialities from use in the short to medium term, France has decided to implement an ambitious action plan to anticipate and avoid withdrawal without alternative solutions. The French wine industry (cniv and ifv) has been heavily involved in this action to define priorities. faced with the risk of the withdrawal of multi-site fungicides (folpel, dithianon, copper) coupled with the probable reduction in single-site fungicide solutions, mildew and black rot have been identified as the priority uses.

Grapevine varietal diversity as mitigation tool for climate change: Agronomic and oenologic potential of 14 foreign varieties grown in Languedoc region (France)

Climate change effects in Languedoc include an expected rise in temperatures, increased evapotranspiration as well as more severe and frequent climatic hazards, such as frost, drought periods and heat waves. For winegrowers theses phenomena impact both yield and quality, resulting in more frequent unbalanced wines. Research on identified mitigation tools for vineyard management is necessary to improve resilience of grapevine agrosystems. Varietal assortment is one of them. This study focuses on agronomic and oenologic potential of 14 foreign varieties grown in Languedoc French region. Fourteen grapevine varieties were monitored during 2021 from June until harvest on eight different sites, some of which occurring on more than one site adding up to 21 different modalities: 7 white varieties Alvarinho B, Assyrtiko B (2), Malvasia Istriana B, Parellada B, Verdejo B, Verdelho B, Xarello B, and 7 black varieties Saperavi N (2), Touriga nacional N, Baga N, Aleatico N, Montepulciano N (2), Primitivo N (3), Calabrese N (3). Varietals were compared through the following parameters: phenology was assessed by using the information collected in the Database Network of French Vine Conservatories (INRAE-SupAgro-IFV, 2005-2015). The number of inflorescences for shoots from secondary buds and bourillons and suckers were observed to assess post-bud break frost tolerance potential. Grapevine water status was studied through stem water potential measurement, observation of foliage symptoms of drought, and 𝛿13C on must. Frequencies and intensities of downy mildew, powdery mildew, and black rot attacks were estimated before harvest on leaves and clusters and botrytis at harvest to assess disease susceptibilities. Berry composition was monitored from end of veraison until harvest. Yield and mean bunch weight were also calculated. Varieties were then ranked on a 1-4 scale for each parameter and compared through PCA. Forty two stations of the Mediterranean basin were compared by PCA with the Multicriteria Climatic Classification indicators in order to confront the collected information during 2021 campaign to the hypothesis that plants coming from dry and hot regions are genetically adapted to such climatic conditions.

Applications pratiques du zonage vitivinicole

Le zonage vitivinicole présente toute une série d’applications pratiques. Son importance est en train d’augmenter, soit en fonction des moyens techniques chaque fois plus performants, qui rendent possible le développement des zonages de plus en plus intégrées, consistants et utiles, soit en fonction d’un marché de plus en plus mondialisé. L’article situe la contribution du zonage au niveau de la production vitivinicole et du développement du territoire.

Influence of Lactiplantibacillus plantarum and Oenococcus oeni strains on sensory profile of sicilian nero d’avola wine after malolactic fermentation.

AIM: Malolactic fermentation is a process of decarboxylation of L-malic acid into L-lactic acid and carbon dioxide that leads to deacidification, modification of odors and flavors of wines [1]