IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Biochemical characterization of grape skin cell wall during ripening in relation to Botrytis cinerea susceptibility of two Champagne cultivars

Biochemical characterization of grape skin cell wall during ripening in relation to Botrytis cinerea susceptibility of two Champagne cultivars

Abstract

Pectins or pectic polysaccharides are one of the major components in grape skin cell wall, they contribute to physiological processes which determine the integrity and rigidity of grape skin tissue. Their composition and organization in the cell wall matrix differ according cultivars and also play an important role in the defense mechanisms against plant pathogen and wounding. During grape ripening, important structural and biochemical changes are modifying the cell wall integrity due to pectolytic enzymes such as pectin methylesterases and polygalacturonases which participate to the cell wall weakening and increase the grape susceptibility to pathogens such as Botrytis cinerea.This work investigated the distribution of pectic polysaccharides in the cell wall according to their molecular weight and the localization of pectins (homogalacturonans) highly and low methyl-esterified in grape skin tissue throughout the berry development of the two main Champagne cultivars (Vitis vinifera cv. Pinot noir and Chardonnay), in relation with in vitro Botrytis cinerea susceptibility tests. The skin cell wall composition was evaluated by size exclusion chromatography (SEC) and the pectin localization by immunogold labelling.The comparative study between the two main grape cultivars from Champagne region highlights differences in pectin composition, Chardonnay skins are characterized by less pectic polysaccharides of high molecular weight (HMW) related to a lower susceptibility to Botrytis cinerea. The pectins cellular localization showed that pectins highly methyl-esterified are more important in Pinot noir cell walls than Chardonnay ones, suggesting different mechanisms of cell walls degradation between Chardonnay and Pinot noir skins.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Andre Marie1, Lacampagne Soizic1, Barsacq Audrey1, Mercier Laurence2 and Gény-Denis Laurence1

1Unité mixte de recherche Œnologie, UMR 1366 Université de Bordeaux, INRAE, Bordeaux INP, ISVV MHCS, Epernay, 33882, Villenave d’Ornon, France
2MHCS, Epernay, France

Contact the author

Keywords

skin, ripening, pectins, SEC, Champagne

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Impact of the ‘Pinot’-family on early ripening in cool climate viticulture varieties

‘Pinot Precoce Noir’ (PPN) is an early ripening clone of ‘Pinot Noir’ (PN). The phenological differentiation is visible by an about two weeks earlier onset of veraison. It was found that the early veraison locus Ver1 on chromosome 16, previously identified in ‘Calardis Musqué’, originated from PPN. A highly correlated SSR marker, namely GF16-Ver1, was developed and tested for its ability to molecularly differentiate between PPN and PN as well as its potential to trace individual descendants.

PAIRING WINE AND STOPPER: AN OLD ISSUE WITH NEW ACHIEVEMENTS

The sensory characteristics of wine are a topic studied by several researchers over time, but it continues to be a current and challenging subject. These characteristics are fundamental for the consumer acceptability, which has increasingly aroused their interest to modulate them in line with current market trends and innovation demands. The wine physical-chemical and sensory properties depend on a wide set of factors: they begin to be designed in the vineyard and are later constructed during the various stages of winemaking. Afterwards, the wine is placed in bottles and stored or commercialized.

On the relationship between climate and “terroir” at different spatial scales: the input of new methodological tools

Un grand nombre de travaux ont été consacrés à la mise en éyidence et à la quantification de l’effet du climat sur la qualité de la production viticole. IIs ont permis de caractériser les grands types de production à une large échelle géographique, et d’en évaluer les variations interannuelles au niveau des millésimes.

The role of ampelographic collection in genetic improvement of native varieties and the creation new varieties

The available plant diversity is maintained in global genetic collections and germplasm banks. One of the main objectives of the study of the genetic material of vine still conducting research to characterize the genotypes and the creation of new varieties. The main ampelographic collection of the country, the largest in the Balkans, is located at the Athens Vine Institute in Lykovrisi, Attica, in an area of 70 acres. It contains more than 800 varieties, most of which are indigenous. The Institute is conducting research on the genetic improvement of native varieties and the creation new winemaking and table grape varieties of high productivity, grape quality, resistance to fungal diseases and their adaptability to stresses using the hybridization method using European high-quality varieties.

The use of elicitors in viticulture: a tool to obtain highly colored wines with a reduce alcohol content?

Climate change is causing a gap between the technological and phenolic maturity of grapes, resulting in wines with high alcohol content and low polyphenol concentration. Another phenomenon associated with high temperatures and whose effect is more pronounced if the harvest is delayed is the decrease in the acidity of the grapes, mainly in malic acid, and an increase in pH caused by the accumulation of potassium derived from the increase in temperature. Therefore, climate change and the effects it causes on the vine leads to unbalanced wines, with high alcohol content and lack of color, with green tannins, astringency and excessively low acidity if not corrected.