IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

Abstract

Extraction of anthocyanins and tannins have been studied for two grape varieties, Carignan and Grenache, two maturation levels and two vintages, in model solutions and in wines, using UHPLC-MS/MS in the MRM mode  and HPSEC. The cell wall polysaccharides were characterized using the neutral sugar composition after depolymerization and the comprehensive microarray polymer profiling (CoMPP).
Carignan was richer than Grenache in anthocyanins for both years. Berry anthocyanins were mainly non acylated and para coumaroylated.  In Carignan, p.coumaroylated were found in higher quantities than non acylated. Maturation led to an increase of quantities of non acylated anthocyanins for Carignan and Grenache, and a slight decrease of p.coumaroylated for Carignan. No significant difference of their tannin composition was observed.
The extraction yields of non acylated anthocyanins in model solutions and in wines were higher than those of tannins. Percents of recoveries of p.coumaroylated anthocyanins were lower than non acylated anthocyanins and tannins, and lower in model solutions than in wine. Recoveries were higher in 2019 than in 2018.
Correlations were observed between non acylated, p.coumaroylated and tannins concentrations in model solutions and wines, not in berries. P.coumaroylated anthocyanins recoveries were lower in model solutions than in wines. The cell wall structure was related to the mechanism of extraction. Extraction of anthocyanins and tannins was correlated to high levels of homogalacturonans partially esterified in the skins (e.g. LM19-CDTA-skin) but low levels in the pulps, and by low levels of extensins in the skin(e.g. JIM11-NaOH-pulp) but high levels in the pulps. Arabinose % was correlated positively, mannose % and glucose % negatively to the recovery of all anthocyanins and tannins in model solution, to p.coumaroylated anthocyanins only in wines. These results trigger questions.
Firstly, the lower recovery of p.coumaroylated anthocyanins may be due to the hydrophobicity of the coumaroyl unit, modifying their interactions with other polyphenols and/or with the cell walls.
Secondly, p.coumaroylated anthocyanin recoveries were very different in model solutions and in wines. Pulp, seeds and/or yeasts present in wines should play a role in their extractibility.
Thirdly, anthocyanins/tannins extracted in model solutions/wines were correlated to several parameters describing the cell walls, among them their compositions measured by the neutral sugars and their structures measured by the CoMPPs. To conclude, this study confirms with more details the major role that play cell walls in the extraction of anthocyanins and tannins.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Boulet Jean-Claude1, Abi-Habib, Carrillo Stéphanie, Roi Stéphanie, Verbaere Arnaud, Meudec Emmanuelle, Rattier Anaïs, Ducasse Marie-Agnès, Jorgensen Bodil, Hansen Jeanett, Le Gall Sophie, Poncet-Legrand Céline, Cheynier Véronique, Doce Thierry and Verneht Aude

1SPO, INRAE, Univ.Montpellier, Institut Agro Montpellier Supagro, 34070 Montpellier, Campus Supagro, Bâtiment 28, 2 Place Viala, 34060 Montpellier cedex 2, France
2INRAE, PROBE infrastructure, PFP facility, 34070 Montpellier, Campus Supagro, Bâtiment 28, 2 Place Viala, 34060 Montpellier cedex 2, France

Contact the author

Keywords

extraction, polyphenols, polysaccharides, comprehensive microarrray polymer profiling, wine

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Complantations : enjeux et facteurs de réussite

Dans le cadre de TerclimPro 2025, Coralie Dewasme a présenté un article IVES Technical Reviews. Retrouvez la présentation ci-dessous ainsi que l’article associé : https://ives-technicalreviews.eu/article/view/8486

Method for the evaluation of climatic changes envisaging the protection of grape-growing terroirs: the Géoviticulture MCC system in the evaluation of the potential impact of the construction of hydroelectric power plants on viticulture

La recherche, conduite en 2002, a envisagé l’estimation, a priori, de l’effet du changement mesoclimatique sur le potentiel qualitatif de la région viticole de la Serra Gaúcha (Vallée du Rio das Antas) – Brésil, en fonction de la construction de 3 usines hydroélectriques.

Methodological advances in relating deep root activity to whole vine physiology

Full understanding of grapevine responses to variable soil resources requires
assessing the grapevine root system. Grapevine root systems are expansive and examining deep roots (i.e., >40 cm)
is particularly important in conditions where grapevines increase reliance on deep soil resources, such as drought
or plant competition. Traditional methods of assessing roots rely on morphological traits associated specific
functions (e.g., root color, diameter, length), while recent methodological advances allow for estimating root
function more directly (e.g., omics). Yet, the potential of applying refined methods remains underexplored for roots
at deep depths.

A new chemiluminescence method related to molecules derived from Botrytis cinerea for characterization of Aszu wines from Tokaj, from Hungary

For the chemical characterization of Aszu wines from Tokaj region our aim is to develop a biochemical method which is related to Botrytis cinerea.

Changes in red wine composition during bottle aging: impacts of viticultural conditions and oxygen availability

Bottle ageing is considered essential for most premium red wine production. An important aim of bottle ageing of wine is to achieve a balance between the oxidative and reductive development. This is typically evaluated by the accumulation of aldehyde compounds (causing oxidative off-flavour) and sulfur-containing compounds (causing reductive off-flavour) in the wine [1]

IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

Abstract

Content of the article

References

Section for all references

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: typeofthepublication

Authors

author1, author2, author3

Presenting author

Description

List of affiliations ¹ ² ³

Contact the author

Email address (with mailto: link)

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Effect of redox mediators on the activity of laccase from Botrytis cinerea against volatile phenols

Volatile phenols namely 4-ethylphenol and 4-ethylguaiacol are formed by enzymatic decarboxylation of hydroxycinnamic acids by Brettanomyces yeasts to give vinylphenols and subsequent reduction of the vinyl group to form the correspondent ethylphenols. The presence of these compounds in wine affects negatively its aromatic quality, conferring unpleasant animal and phenolic odor when present in quantities above the olfactory detection threshold [1]. Several methods have been described to remove these undesirable compounds from wines, including the use laccase enzymes [2, 3]. Due to this, the aim of this work was to evaluate the effect of several natural redox mediators on the activity of Botrytis cinerea laccase against these volatile phenols.

Ozone to improve the implantation of Lachancea thermotolerans for improving pH in warm areas in wines with low SO2 levels

Una de las biotecnologías más potentes para disminuir el pH en vinos de zonas cálidas y en variedades de pH elevado es el uso de la levadura no-saccharomyces lachancea thermotolerans. Esta especie es capaz de formar ácido láctico a partir de azúcares, reduciendo al mismo tiempo ligeramente el grado alcohólico. Por lo tanto, mejora dos de los principales problemas de los vinos de regiones afectadas por el calentamiento global. El ácido láctico es un ácido orgánico con una buena integración sensorial en el sabor del vino, y también química y biológicamente estable durante el envejecimiento del vino.

Comparison between satellite and ground data with UAV-based information to analyse vineyard spatio-temporal variability

Currently, the greatest challenge for vine growers is to improve the yield and quality of grapes by minimizing costs and environmental impacts. This goal can be achieved through a better knowledge of vineyard spatial variability. Traditional platforms such as airborne, satellite and unmanned aerial vehicles (UAVs) solutions are useful investigation tools for vineyard site specific management.

Polyphenols in kombucha: Metabolomic analysis of biotransformations during fermentation

Kombucha is a non-alcoholic beverage made of sugared tea that is transformed by a symbiotic consortium of yeasts and bacteria. This beverage is increasingly produced at industrial scale, but its quality standards remain to be defined. Metabolomics analysis was carried out using FT-ICR-MS to understand the chemical transformations induced by the production phases and the type of tea on

Text mining of wine reviews to investigate quality markers of ‘Nebbiolo’ wines from Valtellina

In Valtellina zone (north Italy), the winemaking of ‘Nebbiolo’ grapes leads to the production of two main wine types: classic red wines from fresh grapes, usually classified as Valtellina Superiore DOCG (mandatory oak aging) or Rosso di Valtellina DOC, and the Sforzato di Valtellina DOCG, which is produced using withered grapes according to traditional product specification and subjected to mandatory oak aging process. The withering process influences grape chemical composition and, in turn, the wine sensory profile, which is strongly linked to the wine quality and typicity perceived by consumers.