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IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

Abstract

Extraction of anthocyanins and tannins have been studied for two grape varieties, Carignan and Grenache, two maturation levels and two vintages, in model solutions and in wines, using UHPLC-MS/MS in the MRM mode  and HPSEC. The cell wall polysaccharides were characterized using the neutral sugar composition after depolymerization and the comprehensive microarray polymer profiling (CoMPP).
Carignan was richer than Grenache in anthocyanins for both years. Berry anthocyanins were mainly non acylated and para coumaroylated.  In Carignan, p.coumaroylated were found in higher quantities than non acylated. Maturation led to an increase of quantities of non acylated anthocyanins for Carignan and Grenache, and a slight decrease of p.coumaroylated for Carignan. No significant difference of their tannin composition was observed.
The extraction yields of non acylated anthocyanins in model solutions and in wines were higher than those of tannins. Percents of recoveries of p.coumaroylated anthocyanins were lower than non acylated anthocyanins and tannins, and lower in model solutions than in wine. Recoveries were higher in 2019 than in 2018.
Correlations were observed between non acylated, p.coumaroylated and tannins concentrations in model solutions and wines, not in berries. P.coumaroylated anthocyanins recoveries were lower in model solutions than in wines. The cell wall structure was related to the mechanism of extraction. Extraction of anthocyanins and tannins was correlated to high levels of homogalacturonans partially esterified in the skins (e.g. LM19-CDTA-skin) but low levels in the pulps, and by low levels of extensins in the skin(e.g. JIM11-NaOH-pulp) but high levels in the pulps. Arabinose % was correlated positively, mannose % and glucose % negatively to the recovery of all anthocyanins and tannins in model solution, to p.coumaroylated anthocyanins only in wines. These results trigger questions.
Firstly, the lower recovery of p.coumaroylated anthocyanins may be due to the hydrophobicity of the coumaroyl unit, modifying their interactions with other polyphenols and/or with the cell walls.
Secondly, p.coumaroylated anthocyanin recoveries were very different in model solutions and in wines. Pulp, seeds and/or yeasts present in wines should play a role in their extractibility.
Thirdly, anthocyanins/tannins extracted in model solutions/wines were correlated to several parameters describing the cell walls, among them their compositions measured by the neutral sugars and their structures measured by the CoMPPs. To conclude, this study confirms with more details the major role that play cell walls in the extraction of anthocyanins and tannins.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Boulet Jean-Claude1, Abi-Habib, Carrillo Stéphanie, Roi Stéphanie, Verbaere Arnaud, Meudec Emmanuelle, Rattier Anaïs, Ducasse Marie-Agnès, Jorgensen Bodil, Hansen Jeanett, Le Gall Sophie, Poncet-Legrand Céline, Cheynier Véronique, Doce Thierry and Verneht Aude

1SPO, INRAE, Univ.Montpellier, Institut Agro Montpellier Supagro, 34070 Montpellier, Campus Supagro, Bâtiment 28, 2 Place Viala, 34060 Montpellier cedex 2, France
2INRAE, PROBE infrastructure, PFP facility, 34070 Montpellier, Campus Supagro, Bâtiment 28, 2 Place Viala, 34060 Montpellier cedex 2, France

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Keywords

extraction, polyphenols, polysaccharides, comprehensive microarrray polymer profiling, wine

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First step in the preparation of a soil map of the Protected Designation of Origin Valdepeñas (Central, Spain)

This work is a first step to make a map of vineyard soils. The characterization of the soils of the Protected Designation of Origin (D.P.O.) Valdepeñas will allow to group the studied profiles according to their physico-chemical characteristics and the concentrations of most relevant chemical elements. 90 soil profiles were analysed throughout the territory and the soils were sampled and described according to FAO (2006) and classified according to and Soil Taxonomy (2014). All samples were air dried, sieved and some physico-chemical parameters were determined following standard protocols. Also, major and trace elements were analysed by X-ray fluorescence. The statistically study was made using the SPSS program. Trend maps were made using the ArcGIS program. The studied soils have the following average properties: pH, 8.3; electrical conductivity, 0,20 dS/m (low); clay, 18.8% (medium) and CaCO3, 17.1% (high). In the study for the major elements. The major elements of these soils are Si, followed by Ca and Al, with an average content of 203.7 g/kg, 105.5 g/kg and 74.0 g/kg respectively. On the other hand, 27 trace elements have been studied. Of all of them, it can be highlighted the average values of Ba (361.8 mg/kg), Sr (129.3 mg/kg), Rb (83.4 mg/kg), V (74.2 mg/kg) and Ce (70.6 mg/kg). Ba, V and Ce values are higher and the values of Sr and Rb are lower to those found in the literature. The discriminant analysis shows a percentage of grouping of 91%. The content of chemical elements together with the physico-chemical characteristics allows grouping the soils in 4 group according to their order in the classification to Soil Taxonomy; due to the importance of the Calcisols in Castilla-La Mancha, it has been decided to establish them as their own group even if they do not appear in Soil Taxonomy classification.

Organic recycled mulches in sustainable viticulture: assessment of spontaneous plants communities and weed coverage

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The development of new generations of multispectral satellites such as Sentinel-2 opens possibilities as to vine water status assessment (Cohen et al., 2019). Based on a three years field campaign, a model of Stem Water Potential (SWP) estimation on vine using four satellite bands in Red, Red-Edge, NIR and SWIR domains was developed (Laroche-Pinel et al., 2021). The model relies on SWP field measures done using a pressure chamber (Scholander et al., 1965), which is a common, robust and precise method to assess vine water status (Acevedo-Opazo et al., 2008). The model was mainly developed from from SWP measures on Syrah N (Laroche Pinel E., 2021).

A large scale monitoring was organized in different vineyards in the Mediterranean region in 2021. 10 varieties amongst the most represented in this area were monitored (Cabernet sauvignon N, Chardonnay B, Cinsault N, Grenache N, Merlot N, Mourvèdre N, Sauvignon B, Syrah N, Vermentino B, Viognier B). The model was used to produce water status maps from Sentinel-2 images, starting from the beginning of June (fruit set) up to September (harvest). The average estimated SWP for each vine was compared to actual field SWP measures done by wine growers or technicians during usual monitoring of irrigation programs. The correlations between mean estimated SWP and mean measured SWP were at the same level than expected by the model. (Laroche Pinel, 2021) The general SWP kinetics were comparable. The estimated SWP would have led to same irrigation decisions concerning the date of first irrigation in comparison with measured SWP.

Acevedo-Opazo, C., Tisseyre, B., Ojeda, H., Ortega-Farias, S., Guillaume, S. (2008). Is it possible to assess the spatial variability of vine water status? OENO One, 42(4), 203.
Cohen, Y., Gogumalla, P., Bahat, I., Netzer, Y., Ben-Gal, A., Lenski, I., … Helman, D. (2019). Can time series of multispectral satellite images be used to estimate stem water potential in vineyards? In Precision agriculture ’19, The Netherlands: Wageningen Academic Publishers, pp. 445–451.
Laroche-Pinel, E., Duthoit, S., Albughdadi, M., Costard, A. D., Rousseau, J., Chéret, V., & Clenet, H. (2021). Towards vine water status monitoring on a large scale using sentinel-2 images. remote sensing, 13(9), 1837.
Laroche-Pinel,E. (2021). Suivi du statut hydrique de la vigne par télédétection hyper et multispectrale. Thèse INP Toulouse, France.
Scholander, P.F., Bradstreet, E.D., Hemmingsen, E.A., & Hammel, H.T. (1965). Sap pressure in vascular plants: Negative hydrostatic pressure can be measured in plants. Science, 148(3668), 339–346.

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IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

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Publication date: June 23, 2022

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Low-cost sensors as a support tool to monitor soil-plant heat exchanges in a Mediterranean vineyard

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Use of a new, miniaturized, low-cost spectral sensor to estimate and map the vineyard water status from a mobile 

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Farming management practices aiming at conserving soil moisture have been developed in arid and semiarid-areas facing water scarcity problems. Organic mulching is an effective method to manipulate the crop-growing microclimate increasing crop yield by controlling soil temperature, and retaining soil moisture by reducing soil evaporation. In this sense, the effectiveness of different organic mulching materials (straw mulch and grapevine pruning debris) applied within the row of a vineyard was evaluated on the soil and on the vine in a Tempranillo vineyard located in La Rioja (Spain). Organic mulches were compared with a traditional bare soil management technique (based on the use of herbicides to avoid weed incidence). Mulching coverages favourably influenced the soil water retention throughout all the grapevine vegetative cycle. However, the soil-moisture variation was not the same under different mulching materials, being the straw mulch (SM) the one that retained more water in comparison with grapevine pruning debris (GPD) based-cover. The changes of soil moisture in the upper surface layer (0–10 cm) were highly dynamic, probably due to water vapour fluxes across the soil-atmospheric interface. However, both, SM and GPD reduced these fluctuations as compared with bare soils. A similar trend occurred with soil temperature. Both organic mulches altered soil temperature in comparison with bare soil by reducing soil temperature in summer and raising it in winter. Moreover, the same buffering effect for the temperature on the covered soil also remains in the deeper layers. To conclude, we could see that organic mulching had a positive impact on soil-moisture storage and soil temperature and the extent of this effect depends on the type of mulching materials. These changes led to higher rates of photosynthesis and stomatal conductivity compared to bare soils, also favouring crop growth and grape yields.

Projected changes in vine phenology of two varieties with different thermal requirements cultivated in La Mancha DO (Spain) under climate change scenarios

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