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IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

Abstract

Extraction of anthocyanins and tannins have been studied for two grape varieties, Carignan and Grenache, two maturation levels and two vintages, in model solutions and in wines, using UHPLC-MS/MS in the MRM mode  and HPSEC. The cell wall polysaccharides were characterized using the neutral sugar composition after depolymerization and the comprehensive microarray polymer profiling (CoMPP).
Carignan was richer than Grenache in anthocyanins for both years. Berry anthocyanins were mainly non acylated and para coumaroylated.  In Carignan, p.coumaroylated were found in higher quantities than non acylated. Maturation led to an increase of quantities of non acylated anthocyanins for Carignan and Grenache, and a slight decrease of p.coumaroylated for Carignan. No significant difference of their tannin composition was observed.
The extraction yields of non acylated anthocyanins in model solutions and in wines were higher than those of tannins. Percents of recoveries of p.coumaroylated anthocyanins were lower than non acylated anthocyanins and tannins, and lower in model solutions than in wine. Recoveries were higher in 2019 than in 2018.
Correlations were observed between non acylated, p.coumaroylated and tannins concentrations in model solutions and wines, not in berries. P.coumaroylated anthocyanins recoveries were lower in model solutions than in wines. The cell wall structure was related to the mechanism of extraction. Extraction of anthocyanins and tannins was correlated to high levels of homogalacturonans partially esterified in the skins (e.g. LM19-CDTA-skin) but low levels in the pulps, and by low levels of extensins in the skin(e.g. JIM11-NaOH-pulp) but high levels in the pulps. Arabinose % was correlated positively, mannose % and glucose % negatively to the recovery of all anthocyanins and tannins in model solution, to p.coumaroylated anthocyanins only in wines. These results trigger questions.
Firstly, the lower recovery of p.coumaroylated anthocyanins may be due to the hydrophobicity of the coumaroyl unit, modifying their interactions with other polyphenols and/or with the cell walls.
Secondly, p.coumaroylated anthocyanin recoveries were very different in model solutions and in wines. Pulp, seeds and/or yeasts present in wines should play a role in their extractibility.
Thirdly, anthocyanins/tannins extracted in model solutions/wines were correlated to several parameters describing the cell walls, among them their compositions measured by the neutral sugars and their structures measured by the CoMPPs. To conclude, this study confirms with more details the major role that play cell walls in the extraction of anthocyanins and tannins.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Boulet Jean-Claude1, Abi-Habib, Carrillo Stéphanie, Roi Stéphanie, Verbaere Arnaud, Meudec Emmanuelle, Rattier Anaïs, Ducasse Marie-Agnès, Jorgensen Bodil, Hansen Jeanett, Le Gall Sophie, Poncet-Legrand Céline, Cheynier Véronique, Doce Thierry and Verneht Aude

1SPO, INRAE, Univ.Montpellier, Institut Agro Montpellier Supagro, 34070 Montpellier, Campus Supagro, Bâtiment 28, 2 Place Viala, 34060 Montpellier cedex 2, France
2INRAE, PROBE infrastructure, PFP facility, 34070 Montpellier, Campus Supagro, Bâtiment 28, 2 Place Viala, 34060 Montpellier cedex 2, France

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Keywords

extraction, polyphenols, polysaccharides, comprehensive microarrray polymer profiling, wine

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IVAS 2022 | IVES Conference Series

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Under-vine management effects on grapevine production, soil properties and plant communities in South Australia

Under-vine (UV) management has traditionally consisted of synthetic herbicide use to limit competition between weeds and grapevines. With growing global interest towards non-synthetic chemical use, this study aimed to capture the effects of alternative UV management at two commercial Shiraz vineyards in South Australia, where the sole management variables were UV management since 2016. In adjacent treatment blocks, cultivation (CU) was compared to spontaneous vegetation (SV) in McLaren Vale (MV), and herbicide was compared to SV in Eden Valley (EV). Soil water infiltration rates were slower and grapevine stem water potential was lower in CU compared to SV in MV, with the latter having a plant community dominated by soursob (Oxalis pes-caprae) during winter; while in EV, there was little separation between the treatments. Yields were affected at both sites, with SV being higher in MV and HE being higher in EV. In MV, the only effect on grape must was a lower 13C:12C isotope ratio in CU, indicating greater grapevine water stress. In the grape must at EV, SV had higher total soluble solids, total phenolics, anthocyanins, and yeast available nitrogen; and lower pH and titratable acidity. Pruning weights were not affected by the treatments in MV, while they were higher in HE at EV. Assessments revealed that the differing soil types at the two sites were likely the main determinants of the opposing production outcomes associated with UV management. In the silty loam soil of MV, the higher yields in SV were likely due to more plant-available water, as a potential result of the continuous soil bio-pores formed by winter UV vegetation. Conversely, in the loamy sand soils of EV with a lower cation exchange capacity, the lower yields and pruning weights in SV suggest the UV vegetation competed significantly with the grapevines for available water and nutrients.

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IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

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Publication date: June 23, 2022

Issue: IVAS 2022

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