IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 New fungus-resistant grapevine varieties display high and drought-independent thiol precursor levels

New fungus-resistant grapevine varieties display high and drought-independent thiol precursor levels

Abstract

The use of varieties tolerant to diseases is a long-term but promising option to reduce chemical input in viticulture. Several important breeding programs in Europe and abroad are starting to release a range of new hybrids performing well regarding fungi susceptibility and wine quality. Unfortunately, little attention is paid by the breeders to the adaptation of these varieties to climatic changes and to the aromatic potential such as thiol precursors. Indeed, varietal thiols (3-sulfanylhexan-ol (3SH) and its acetate or the 4-methyl-4-sulfanylpentan-2-one (4MSP)) are very powerful aromatic compounds in wines coming from odorless precursors in grapes and could contribute to the typicity of such varieties. This study aimed to characterize 6 new resistant varieties selected by INRAE (Floreal, G5 and 3159B for white grapes and Artaban, 3176N and G14 for red grapes) in comparison to Syrah to (i) quantify the thiol precursors in the fruits and to (ii) evaluate the influence of water deficit (WD) imposed on field-grown vines on these molecules. Grapes were picked-up at the arrest of phloem unloading to objectify the sampling at a precise physiological landmark and analyzed by LC-MS/MS. Six thiol precursors were quantified by isotopic dilution across all samples and only 3 were clearly identified and quantified: the glutathionylated (G3SH), cysteinylated (Cys3SH) and one dipeptidic precursors of 3SH (CysGly3SH). For all varieties, G3SH contents represented between 75 and 100% of the aromatic potential, followed by Cys3SH (0-16%) and finally the CysGly3SH (0-13%). The absolute concentrations of G3SH ranged from 31 to 132 µg/kg for white varieties and from 68 to 466 µg/kg for red ones. Surprisingly, 3176N had exceptional G3SH levels that can reach 466 µg/kg which corresponded to nearly 777 µg/L in volume concentration. The pedigree of this variety which includes Grenache as a progenitor could explain the high levels of thiol precursors as observed in the Rosé wines of Provence, a type of wines also characterized by high levels of varietal thiols. Whatever the variety, we did not find marked effects of WD on the contents in thiol precursors when expressed in µg/kg. When expressed in µg/berry to reflect the real impact of WD on rate of metabolite accumulation per organ, 3176N and Artaban showed significant differences between moderate and high WD treatments (p-value < 0.05, less amount of thiol precursors in WD grapes). Analyzing thiol precursors and more generally metabolites of interest in fruits requires to objectify the sampling date at a given physiological stage. This allows deciphering the effects of environmental factors on the accumulation of metabolites at organ or plant level and their consequences in the concentration of the fruit at harvest. In conclusion, resistant varieties seemed to be less impacted by WD than Vinifera ones, which is bode well for the development of these varieties in relation to climate change challenges.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Roland Aurélie1, Wilhelm Luciana2,3,4, Torregrosa Laurent2,3, Dournes Gabriel4, Pellegrino Anne3 and Ojeda Hernán2

1 SPO, Univ Montpellier, INRAE, Institut Agro, Montpellier, France
2 UE Pech Rouge, INRAE, Gruissan, France
3 UMR LEPSE, Montpellier Uni – CIRAD – INRAE – Institut Agro, Montpellier, France
4 UMR AGAP, Montpellier Uni – CIRAD – INRAE – Institut Agro, Montpellier, France  

Contact the author

Keywords

Climate change, water deficit, tolerant varieties, wine quality, thiol precursors

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…

Adaptation to soil and climate through the choice of plant material

Choosing the rootstock, the scion variety and the training system best suited to the local soil and climate are the key elements for an economically sustainable production of wine. The choice of the rootstock/scion variety best adapted to the characteristics of the soil is essential but, by changing climatic conditions, ongoing climate change disrupts the fine-tuned local equilibrium. Higher temperatures induce shifts in developmental stages, with on the one hand increasing fears of spring frost damages and, on the other hand, ripening during the warmest periods in summer. Expected higher water demand and longer and more frequent drought events are also major concerns. The genetic control of the phenotypes, by genomic information but also by the epigenetic control of gene expression, offers a lot of opportunities for adapting the plant material to the future. For complex traits, genomic selection is also a promising method for predicting phenotypes. However, ecophysiological modelling is necessary to better anticipate the phenotypes in unexplored climatic conditions Genetic approaches applied on parameters of ecophysiological models rather than raw observed data are more than ever the basis for finding, or building, the ideal varieties of the future.

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Soil, vine, climate change – what is observed – what is expected

To evaluate the current and future impact of climate change on Viticulture requires an integrated view on a complex interacting system within the soil-plant-atmospheric continuum under continuous change. Aside of the globally observed increase in temperature in basically all viticulture regions for at least four decades, we observe several clear trends at the regional level in the ratio of precipitation to potential evapotranspiration. Additionally the recently published 6th assessment report of the IPCC (The physical science basis) shows case-dependent further expected shifts in climate patterns which will have substantial impacts on the way we will conduct viticulture in the decades to come.
Looking beyond climate developments, we observe rising temperatures in the upper soil layers which will have an impact on the distribution of microbial populations, the decay rate of organic matter or the storage capacity for carbon, thus affecting the emission of greenhouse gases (GHGs) and the viscosity of water in the soil-plant pathway, altering the transport of water. If the upper soil layers dry out faster due to less rainfall and/or increased evapotranspiration driven by higher temperatures, the spectral reflection properties of bare soil change and the transport of latent heat into the fruiting zone is increased putting a higher temperature load on the fruit. Interactions between micro-organisms in the rhizosphere and the grapevine root system are poorly understood but respond to environmental factors (such as increased soil temperatures) and the plant material (rootstock for instance), respectively the cultivation system (for example bio-organic versus conventional). This adds to an extremely complex system to manage in terms of increased resilience, adaptation to and even mitigation of climate change. Nevertheless, taken as a whole, effects on the individual expressions of wines with a given origin, seem highly likely to become more apparent.

Permanent cover cropping with reduced tillage increased resiliency of wine grape vineyards to climate change

Majority of California’s vineyards rely on supplemental irrigation to overcome abiotic stressors. In the context of climate change, increases in growing season temperatures and crop evapotranspiration pose a risk to adaptation of viticulture to climate change. Vineyard cover crops may mitigate soil erosion and preserve water resources; but there is a lack of information on how they contribute to vineyard resiliency under tillage systems. The aim of this study was to identify the optimum combination of cover crop sand tillage without adversely affecting productivity while preserving plant water status. Two experiments in two contrasting climatic regions were conducted with two cover crops, including a permanent short stature grass (P. bulbosa hybrid), barley (Hordeum spp), and resident vegetation under till vs. no-till systems in a Ruby Cabernet (V. vinifera spp.) (Fresno) and a Cabernet Sauvingon (Napa) vineyard. Results indicated that permanent grass under no-till preserved plant available water until E-L stage 17. Consequently, net carbon assimilation of the permanent grass under no-till system was enhanced compared to those with barley and resident vegetation. On the other hand, the barley under no-till system reduced grapevine net carbon assimilation during berry ripening that led to lower content of nonstructural carbohydrates in shoots at dormancy. Components of yield and berry composition including flavonoid profile at either site were not adversely affected by factors studied. Switching to a permanent cover crop under a no-till system also provided a 9% and 3% benefit in cultural practices costs in Fresno and Napa, respectively. The results of this work provides fundamental information to growers in preserving resiliency of vineyard systems in hot and warm climate regions under context of climate change.