IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 New fungus-resistant grapevine varieties display high and drought-independent thiol precursor levels

New fungus-resistant grapevine varieties display high and drought-independent thiol precursor levels

Abstract

The use of varieties tolerant to diseases is a long-term but promising option to reduce chemical input in viticulture. Several important breeding programs in Europe and abroad are starting to release a range of new hybrids performing well regarding fungi susceptibility and wine quality. Unfortunately, little attention is paid by the breeders to the adaptation of these varieties to climatic changes and to the aromatic potential such as thiol precursors. Indeed, varietal thiols (3-sulfanylhexan-ol (3SH) and its acetate or the 4-methyl-4-sulfanylpentan-2-one (4MSP)) are very powerful aromatic compounds in wines coming from odorless precursors in grapes and could contribute to the typicity of such varieties. This study aimed to characterize 6 new resistant varieties selected by INRAE (Floreal, G5 and 3159B for white grapes and Artaban, 3176N and G14 for red grapes) in comparison to Syrah to (i) quantify the thiol precursors in the fruits and to (ii) evaluate the influence of water deficit (WD) imposed on field-grown vines on these molecules. Grapes were picked-up at the arrest of phloem unloading to objectify the sampling at a precise physiological landmark and analyzed by LC-MS/MS. Six thiol precursors were quantified by isotopic dilution across all samples and only 3 were clearly identified and quantified: the glutathionylated (G3SH), cysteinylated (Cys3SH) and one dipeptidic precursors of 3SH (CysGly3SH). For all varieties, G3SH contents represented between 75 and 100% of the aromatic potential, followed by Cys3SH (0-16%) and finally the CysGly3SH (0-13%). The absolute concentrations of G3SH ranged from 31 to 132 µg/kg for white varieties and from 68 to 466 µg/kg for red ones. Surprisingly, 3176N had exceptional G3SH levels that can reach 466 µg/kg which corresponded to nearly 777 µg/L in volume concentration. The pedigree of this variety which includes Grenache as a progenitor could explain the high levels of thiol precursors as observed in the Rosé wines of Provence, a type of wines also characterized by high levels of varietal thiols. Whatever the variety, we did not find marked effects of WD on the contents in thiol precursors when expressed in µg/kg. When expressed in µg/berry to reflect the real impact of WD on rate of metabolite accumulation per organ, 3176N and Artaban showed significant differences between moderate and high WD treatments (p-value < 0.05, less amount of thiol precursors in WD grapes). Analyzing thiol precursors and more generally metabolites of interest in fruits requires to objectify the sampling date at a given physiological stage. This allows deciphering the effects of environmental factors on the accumulation of metabolites at organ or plant level and their consequences in the concentration of the fruit at harvest. In conclusion, resistant varieties seemed to be less impacted by WD than Vinifera ones, which is bode well for the development of these varieties in relation to climate change challenges.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Roland Aurélie1, Wilhelm Luciana2,3,4, Torregrosa Laurent2,3, Dournes Gabriel4, Pellegrino Anne3 and Ojeda Hernán2

1 SPO, Univ Montpellier, INRAE, Institut Agro, Montpellier, France
2 UE Pech Rouge, INRAE, Gruissan, France
3 UMR LEPSE, Montpellier Uni – CIRAD – INRAE – Institut Agro, Montpellier, France
4 UMR AGAP, Montpellier Uni – CIRAD – INRAE – Institut Agro, Montpellier, France  

Contact the author

Keywords

Climate change, water deficit, tolerant varieties, wine quality, thiol precursors

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

Legacy of land-cover changes on soil erosion and microbiology in Burgundian vineyards

Soils in vineyards are recognized as complex agrosystems whose characteristics reflect complex interactions between natural factors (lithology, climate, slope, biodiversity) and human activities. To date, most of the unknown lies in an incomplete understanding of soil ecosystems, and specifically in the microbial biodiversity even though soil microbiota is involved in many key functions, such as nutrient cycling and carbon sequestration. Soil biological properties are indicative of soil quality. Therefore, understanding how soil communities are related to soil ecosystem functioning is becoming an essential issue for soil strategy conservation. Here, we propose to assess the importance of land-cover history on the present-day microbiological and physico-chemical properties. The studied area was selected in the Burgundian vineyards (Pernand-Vergelesses, Burgundy, France) where land occupation has been reconstructed over the last 40 years. Soil samples were collected in five areas reflecting various land cover history (forest, vineyards, shifting from forest to vineyards). For each area, physico-chemical parameters (pH, C, N, P, grain size) were measured and DNA was extracted to characterize the abundance and diversity of microbial communities. The obtained results show significant differences in the five areas suggesting that present-day microbial molecular biomass and bacterial taxonomic is partly inherited from past land occupation. Over longer period of time, such study of land-uses legacies may help to better assess ecosystem recovery and the impact of management practices for a better soil quality and vineyards sustainability.

Geospatial trends of bioclimatic indexes in the topographically complex region of Barolo DOCG

Barolo DOCG is an economically important wine producing region in Northwest Italy. It is a small region of approximately 70 km2 gross area. The topography is very complex with steep sloped hills ranging in elevation from below 200 m to 550 m. Barolo DOCG wine is made exclusively from the Nebbiolo grape. Bioclimatic indexes are often used in viticulture to gain a better understanding of broader climate trends which can be compared temporally and geographically. These indexes are also used for identifying potential phenological timing, growing region suitability, and potential risks associated with expected climatic changes. Understanding how topography influences bioclimatic indexes can help with understanding of mesoscale climate behaviour leading to improved decision making and risk management strategies. The average monthly maximum and minimum temperatures, the Cool Night Index, the Huglin Index, and the monthly diurnal range (from July to October) were calculated using data from 45 weather stations within a 40 km radius of the Barolo DOCG growing area between the years 1996 and 2019. Linear and multiple regression models were developed using independent variables (elevation, aspect, slope) extracted from a digital elevation model to identify significant relationships. Bioclimatic indexes were then kriged with external drift using independent variables that showed significant relationships with the bioclimatic index using a 100 m resolution grid. The maximum monthly temperatures and the Huglin Index showed consistent significant negative relationships with elevation in all years. The minimum monthly temperatures showed no relationship with elevation but in some months a small but significant relationship was observed with aspect. Due to the lack of a relationship between minimum monthly temperatures and elevation compared to the significant relationship between maximum monthly temperatures and elevation, monthly diurnal range had a negative relationship with elevation.

Grapevine xylem embolism resistance spectrum reveals which varieties have a lower mortality risk in a future dry climate

Wine growing regions have recently faced intense and frequent droughts that have led to substantial economical losses, and the maintenance of grapevine productivity under warmer and drier climate will rely notably on planting drought-resistant cultivars. Given that plant growth and yield depend on water transport efficiency and maintenance of photosynthesis, thus on the preservation of the vascular system integrity during drought, a better understanding of drought-related hydraulic traits that have a significant impact on physiological processes is urgently needed. We have worked towards this end by assessing vulnerability to xylem embolism in 30 grapevine commercial varieties encompassing red and white Vitis vinifera varieties, hybrid varieties characterized by a polygenic resistance for powdery and downy mildew, and commonly used rootstocks. These analyses further allowed a global assessment of wine regions with respect to their varietal diversity and resulting vulnerability to stem embolism. Hybrid cultivars displayed the highest vulnerability to embolism, while rootstocks showed the greatest resistance. Significant variability also arose among Vitis vinifera varieties, with Ψ12 and Ψ50 values ranging from -0.4 to -2.7 MPa and from -1.8 to -3.4 MPa, respectively. Cabernet franc, Chardonnay and Ugni blanc featured among the most vulnerable varieties while Pinot noir, Merlot and Cabernet Sauvignon ranked among the most resistant. In consequence, wine regions bearing a significant proportion of vulnerable varieties, such as Poitou-Charentes, France and Marlborough, New Zealand, turned out to be at greater risk under drought. These results highlight that grapevine varieties may not respond equally to warmer and drier conditions, outlining the importance to consider hydraulic traits associated with plant drought tolerance into breeding programmes and modeling simulations of grapevine yield maintenance under severe drought. They finally represent a step forward to advise the wine industry about which varieties and regions would have the lowest risk of drought-induced mortality under climate change.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…