IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The kinetics of grape aromatic precursors hydrolysis at three different temperatures

The kinetics of grape aromatic precursors hydrolysis at three different temperatures

Abstract

In neutral grapes, it is known that most aroma compounds are present as non-volatile precursors. There is strong evidence that supports the existence of a connection between the content of aroma precursors in grapes and the aromatic quality of wine1. Harsh acid hydrolysis is considered the better way to reveal the aroma potential of winemaking grapes, because transformations taking place during fermentation include relevant chemical rearrangements in acid media that are better predicted by acid hydrolysis2. The aim of the present work is to establish a methodology to evaluate the aromatic potential of the grape from the acid hydrolysis in anoxic conditions of its aromatic and phenolic fraction.
In this work, firstly two different samples of Grenache grapes aromatic and phenolic fraction (PAF) were extracted, followed by acid hydrolysis under strict anoxic conditions based on a previously developed methodology3. These PAFs were reconstituted in model wine and aged in duplicate under anoxic conditions at three temperatures: 75, 50 and 35 ºC. The aged model wines were collected at different sampling times 75 ºC (1h, 2h, 6h, 12h, 24h, 48h, 96h), 50 ºC (0.5, 1, 2, 5, 7, 10 and 14 weeks) and at 35ºC (0.5, 1, 5, 3.5, 6, 9 and 12 months).
Hydrolysates were extracted and analyzed by two different analytical methods: esters, free norisoprenoids, terpenoids, phenols, lactones, vanillins and cinnamates were analyzed by SPE-GC-MS4, while varietal thiols were analyzed by LC-QqQ-MS.
The hydrolysates obtained at 50 and 75ºC present sensory profiles congruent with olfactory nuances of unoxidized wine. In fact, the absolute concentration values ​​found for terpenes, lactones and norisoprenoids are within the normal values ​​expected in a wine, except for TDN, which appears in large quantities. However, phenols, derivatives of vanilla and varietal thiols especially 3-mercaptohexanol appear in much higher amounts than would be expected in a Grenache wine, possibly because this type of hydrolysis is capable of release a major part of aromatic potential of grape. Very few differences are observed in the hydrolysis profiles between the two samples. The hydrolysis profile at the same temperature is similar between the samples in most cases even though different amounts of volatiles are obtained. All compounds seem to hydrolyze following two types of behavior that can be explained by the combination of two phenomena: the generation of volatiles (hydrolysis and rearrangements) and the subsequent degradation at wine pH. For those compounds with congruent evolutions at the three different temperatures, a model able to predict the evolution of varietal volatiles at room temperature will be presented.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Sánchez Acevedo Elayma1, Lopez Ricardo1 and Ferreira Vicente1

1Laboratory for Aroma Analysis and Enology (LAAE), Universidad de Zaragoza, Instituto Agroalimentario de Aragón (IA2) (UNIZAR-CITA), Zaragoza (Spain)

Contact the author

Keywords

aromatic and phenolic fraction (PAF), acid hydrolisis, aroma

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Photoselective shade films affect grapevine berry secondary metabolism and wine composition

Grapevine physiology and production are challenged by forecasted increases in temperature and water deficits. Within this scenario, photoselective overhead shade films are promising tools in warm viticulture areas to overcome climate change related factors. The aim of this study was to evaluate the vulnerability of ‘Cabernet Sauvignon’ grape berry to solar radiation overexposure and optimize shade film use for berry integrity. A randomized complete block design field study was conducted across two years (2020-2021) in Oakville, Napa Valley, CA, with four shade films (D1, D3, D4, D5) differing in the percent of radiation spectra transmitted and compared to an uncovered control (C0). Integrals for gas exchange parameters and mid-day stem water potential were unaffected by the shade films in 2020 and 2021. By harvest, berries from uncovered and shaded vines did not differ in their size or primary metabolism in either year. Despite precipitation exclusion during the dormant season in the shaded treatments, yield did not differ between them and the control in either season. In 2020, total skin anthocyanins (mg/g fresh mass) in the shaded treatments was greater than C0 during berry ripening and at harvest. Conversely, flavonol concentrations in 2020 were reduced in shaded vines compared to C0. The 2020 growing season highlighted the impact of heat degradation on flavonoids. Flavonoid concentrations in 2021 increased until harvest while flavonoid degradation was apparent from veraison to harvest in 2020 across shaded and control vines. Wine analyses highlighted the importance of light spectra to modify wine composition. Wine color intensity, tonality and anthocyanin values were enhanced in D4 whereas antioxidant properties were enhanced in C0 and D5 wines. Altogether, our results highlighted the need of new approaches in warm viticulture areas given the impact that composition of light has on berry and wine quality.

Drought effect on aromatic and phenolic potential of seven recovered grapevine varieties in Castilla-La Mancha region (Spain)

The effects of climate change are seriously affecting the quality of wine grapes. High temperatures and drought cause imbalances in the chemical composition of grapes. The result is overripe grapes with low acidity and high sugar content, which produce wines with excessive alcohol content, lacking in freshness and not very aromatic. As a consequence, the search of varieties with capacity of produce quality grapes in adverse climate conditions is a good alternative to preserve the sustainability of vineyards. In this work, quality parameters of seven Vitis vinifera L. cultivars (five whites and two reds) recently recovered from extinction and grown under two different hydric regimes (rainfed and irrigated) were analyzed during the 2020 vintage. At harvest time, weight of 100 berries, must physicochemical parameters (brix degree, total acidity, malic acid, pH), and carbon and oxygen isotope ratios (δ13C, δ18O) were determined. Subsequently, varietal aroma potential index (IPAv) and total polyphenol index (TPI) were analyzed. Quality parameters, IPAv and TPI, showed significant differences between varieties and water regimes. Both red varieties, Moribel and Tinto Fragoso, stood out for their high aromatic and phenolic potential, which was higher under rainfed regime. Regarding to white varieties, Montonera del Casar and Jarrosuelto stood out in terms of varietal aroma potential. Montonera del Casar high acidity in its musts and Jarrosuelto showed the highest berry weights.

Assessing the relationship between cordon strangulation, dieback, and fungal trunk disease symptom expression

Grapevine trunk diseases including Eutypa dieback are a major factor in the decline of vineyards and may lead to loss of productivity, reduced income, and premature reworking or replanting. Several studies have yielded results indicating that vines may be more likely to express symptoms of vascular disease if their health is already compromised by stress. In Australia and many other wine-growing regions it is a common practice for canes to be wrapped tightly around the cordon wire during the establishment of permanent cordon arms. It is likely that this practice may have a negative effect on health and longevity, as older cordons that have been trained in this manner often display signs of decay and dieback, with the wire often visibly embedded within the wood of the cordon. It is possible that adopting a training method which avoids constriction of the vasculature of the cordon may help to limit the onset of vascular disease symptom expression. A survey was conducted during the spring of two consecutive growing seasons on vineyards in South Australia displaying symptoms of Eutypa lata infection when symptomless shoots were 50–100 cm long. Vines were assessed as follows: (i) the proportion of cordon exhibiting dieback was rated using a 0–100% scale; (ii) the proportion of canopy exhibiting foliar symptoms of Eutypa dieback was rated using a 0–100% scale; (iii) the severity of strangulation was rated using a 0–4 point scale. Images were also taken of each vine for the purpose of measuring plant area index (PAI) using the VitiCanopy App. The goal of the survey was to determine if and to what extent any correlation exists between severity of strangulation and cordon dieback, in addition to Eutypa dieback foliar symptom expression.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…

Impact of climate variability and change on grape yield in Italy

Viticulture is entangled with weather and climate. Therefore, areas currently suitable for grape production can be challenged by climate change. Winegrowers in Italy already experiences the effect of climate change, especially in the form of warmer growing season, more frequent drought periods, and increased frequency of weather extremes.
The aim of this study is to investigate the impact of climate variability and change on grape yield in Italy to provide winegrowers the information needed to make their business more sustainable and resilient to climate change. We computed a specific range of bioclimatic indices, selected by the International Organisation of Vine and Wine (OIV), and correlated them to grape yield data. We have worked in collaboration with some wine consortiums in northern and central Italy, which provided grape yield data for our analysis.
Using climate variables from the E-OBS dataset we investigate how the bioclimatic indices changed in the past, and the impact of this change on grape productivity in the study areas. The climate impact on productivity is also investigated by using high-resolution convection-permitting models (CPMs – 2.2 horizontal resolution), with the purpose of estimating productivity in future emission scenarios. The CPMs are likely the best available option for this kind of impact studies since they allow a better representation of small-scale processes and features, explicitly resolve deep convection, and show an improved representation of extremes. In our study, we also compare CPMs with regional climate models (RCMs – 12 km horizontal resolution) to assess the added value of high-resolution models for impact studies. Further development of our study will lead to assessing the future suitability for vine cultivation and could lead to the construction of a statistical model for future projection of grape yield.