IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Use of a recombinant protein (Harpin αβ) as a tool to improve phenolic composition in wines

Use of a recombinant protein (Harpin αβ) as a tool to improve phenolic composition in wines

Abstract

Climate change is modifying environmental conditions in all wine-growing areas of the world. High temperatures are the cause of an increased imbalance between industrial maturity and phenolic maturity, resulting in berries with high sugar levels, low concentration in organic acids and reduced concentrations in aromas and phenolic compounds. These grapes produce wines with high alcohol content and lack freshness, color intensity, and aromatic complexity. Viticultural strategies have been developed in recent years in order to maintain the quality of red wines, with a two-fold objective: improve the phenolic composition of wines and reduce their alcoholic content. Harpin αβ is a recombinant protein and elicitor of hypersensitive responses. When Harpin αβ is applied to crops, the expression of growth and defense genes is stimulated. These genes are generally associated with metabolic signals and pathways related to functions of protein and sugar transport and vegetative development. The objective of this work has been to apply Harpin αβ to the vines after veraison in order to advance harvest for reducing the alcohol content of the final wines while maintaining or improving their phenolic composition compared to full maturity grapes. This experiment was carried out in a commercial vineyard sited in Jumilla (Spain). Three treatments were applied; i) Control: untreated application of Harpin harvested at 15º Baume, ii) 2T: grapes treated twice with Harpin, at the time of veraison and 15 days later and harvested at 13º Baume and iii) 3T: an extra application of the compound made 15 days after the second treatment and grapes were also harvested at 13º Baume. In each treatment, the dose of Harpin αβ applied was 150 g/ha. All treatments were vinified in the same way. Once the wines were bottled, the physicochemical and chromatic parameters were analyzed. Wines from grapes of 2T treatments harvested at 13º Baume decrease significantly the pH, color intensity and total phenolic index of the wines. No significant difference was observed in the total acidity parameter. On the other hand, 3T treatmen increased significantly total anthocyanins compare to control wines. Moreover, this treatment obtained the highest concentration of tannins, although these differences were not significant compared to the control treatment. It is clear that the 3T treatment was much more effective in improving the phenolic concentration of the wines than the 2T treatment. These results showed that the Harpin application in the vineyard (3T) produced wines with similar phenolic content that the wines produced from fully ripe grapes but with 20% less alcohol. This makes the use of Harpin αβ an interesting strategy for winemakers seeking a natural reduction of alcoholic content in their wines without losing quality.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Martínez-Moreno Alejandro ¹, Martínez-Pérez Pilar ¹, Bautista-Ortin Ana Belén¹ Pérez-Porras Paula¹ and Gomez-Plaza Encarna ¹

¹Department of Food Science and Technology, Faculty of Veterinary Sciences, University of Murcia

Contact the author

Keywords

climate change, elicitors, grape ripening, alcohol

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Effects of graft quality on growth and grapevine-water relations

Climate change is challenging viticulture worldwide compromising its sustainability due to warmer temperatures and the increased frequency of extreme events. Grafting Vitis vinifera L.

Permanent cover cropping with reduced tillage increased resiliency of wine grape vineyards to climate change

Majority of California’s vineyards rely on supplemental irrigation to overcome abiotic stressors. In the context of climate change, increases in growing season temperatures and crop evapotranspiration pose a risk to adaptation of viticulture to climate change. Vineyard cover crops may mitigate soil erosion and preserve water resources; but there is a lack of information on how they contribute to vineyard resiliency under tillage systems. The aim of this study was to identify the optimum combination of cover crop sand tillage without adversely affecting productivity while preserving plant water status. Two experiments in two contrasting climatic regions were conducted with two cover crops, including a permanent short stature grass (P. bulbosa hybrid), barley (Hordeum spp), and resident vegetation under till vs. no-till systems in a Ruby Cabernet (V. vinifera spp.) (Fresno) and a Cabernet Sauvingon (Napa) vineyard. Results indicated that permanent grass under no-till preserved plant available water until E-L stage 17. Consequently, net carbon assimilation of the permanent grass under no-till system was enhanced compared to those with barley and resident vegetation. On the other hand, the barley under no-till system reduced grapevine net carbon assimilation during berry ripening that led to lower content of nonstructural carbohydrates in shoots at dormancy. Components of yield and berry composition including flavonoid profile at either site were not adversely affected by factors studied. Switching to a permanent cover crop under a no-till system also provided a 9% and 3% benefit in cultural practices costs in Fresno and Napa, respectively. The results of this work provides fundamental information to growers in preserving resiliency of vineyard systems in hot and warm climate regions under context of climate change.

Analysis of some environmental factors and cultural practices that affect the production and quality of the Manto Negro, Callet and Prensal Blanc varieties

45 non irrigated vineyards distributed in the DO (Denomination) Pla i Llevant de Mallorca and the DO Binissalem Mallorca were used to investigate the characteristics of production and quality and their relationships certain environmental factors and cultural practices. The grape varieties investigated are autochthonous to the island of Mallorca, Manto Negro and Callet as red and Prensal Blanc as white. All plants were measured for four consecutive years in the main production and quality parameters. Among the environmental factors, the type of soil has been studied, more specifically its water retention capacity, the planting density, the age of the vineyard and the level of viral infection. The presence or absence of virus seems to have no effect on any component studied in the varieties studied. For the white variety Prensal Blanc age is negatively correlated with production and the number of bunches, nevertheless it does not cause any effect on the required quality parameters. However, for the red varieties Callet and Manto Negro, the age of the plantation is the variable that best correlates with the quality parameters, therefore the old vines should be the object of preservation by the viticulturists and winemakers in order to guarantee its contribution to the quality of the wines made with these varieties.

Aromatic maturity is a cornerstone of terroir expression in red wine

Harvesting grapes at adequate maturity is key to the production of high-quality red wines. Enologists and wine makers define several types of maturity, including technical maturity, phenolic maturity and aromatic maturity. Technical maturity and phenolic maturity are relatively well documented in the scientific literature, while articles on aromatic maturity are scarcer. This is surprising, because aromatic maturity is, without a doubt, the most important of the three in determining wine quality and typicity (including terroir expression). Optimal terroir expression can be obtained when the different types of maturity are reached at the same time, or within a short time frame. This is more likely to occur when the ripening takes place under mild temperatures, neither too cool, nor too hot. Aromatic expression in wine can be driven, from low to high maturity, by green, herbal, fresh fruit, ripe fruit, jammy fruit, candied fruit or cooked fruit aromas. Green and cooked fruit aromas are not desirable in red wines, while the levels of other aromatic compounds contribute to the typicity of the wine in relation to its origin. Wines produced in cool climates, or on cool soils in temperate climates, are likely to express herbal or fresh fruit aromas; while wines produced under warm climates, or on warm soils in temperate climates, may express ripe fruit, jammy fruit or candied fruit aromas. Growers can optimize terroir expression through their choice of grapevine variety. Early ripening varieties perform better in cool climates and late ripening varieties in warm climates. Additionally, maturity can be advanced or delayed by different canopy management practices or training systems.