IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Does Dekkera/Brettanomyces wine spoilage raise the risk of biogenic amines intake? A screening in Portuguese red wines

Does Dekkera/Brettanomyces wine spoilage raise the risk of biogenic amines intake? A screening in Portuguese red wines

Abstract

Wine quality and safety are the main concerns of consumers and health agencies. Biogenic amines and polyamines, depending on their concentration and on individuals, in wine can constitute a potential public health concern due to their physiological and toxicological effects. Biogenic amines can be present in grapes, such as putrescine, spermidine, and spermine [1] or formed by microorganisms during the winemaking process such as histamine, cadaverine, hexylamine, and ethylamine [2]. Histamine is one of the targeted toxins by the Food and Drug Administration and the European Food Safety Authority. Dekkera/Brettanomyces, a wine spoilage yeast, can produce biogenic amines in grape juice [3]. Diamines can produce carcinogenic nitrosamines by reaction with nitrite. Biogenic amines are important causes of wine intolerance [4], producing intoxication symptoms.
The sensitivity to biogenic amines depends on insufficient amino oxidase activity, genetic predisposition, alcohol, acetaldehyde, gastrointestinal disease, or inhibition by other amines. Furthermore, it is worth mentioning that, susceptible persons who are immune-compromised and seniors, may exhibit intolerance to even low levels of biogenic amines and suffer more severe symptoms, these persons are increasing in developed countries. Therefore, factors that influence biogenic amines concentrations are of utmost importance for consumer safety, mainly for susceptible persons. The main objective of this study was to quantify biogenic amines and polyamines in industrially produced red wines available in Portuguese wineries. As well as to understand the impact of the spoilage yeasts Dekkera/Brettanomyces in the biogenic amines and polyamines concentrations. Wine sampling was carried out using a maximum variance/heterogeneous purposive non-probability technique. Ethylphenols were determined by GC-MS and biogenic amines and polyamines were determined by dispersive solid-phase extraction and HPLC-DAD after derivatization with benzoyl chloride. To better understand the real input of Dekkera/Brettanomyces activity in these compounds, a set of 79 Portuguese red wines produced at an industrial scale from 2012 to 2016 vintage were analyzed. A total of nine amines have been detected that range from 19.6 to 331 mg/L and concentrations of 4-ethylphenol of 4.5–5604 μg/L and of 4-ethylguaiacol of 2.3–831.2 μg/L [5]. The most abundant amines on average were putrescine followed by histamine and cadaverine. Simultaneous determination of biogenic amines and volatile phenols in industrial produced red wines permitted to conclude that the wine spoilage activity of Dekkera/Brettanomyces with the production of volatile phenols do not significantly contribute to biogenic amines increase and consequently intake by the consumers. Biogenic amines need to be controlled in order to ensure high levels of wine safety and quality to reduce risk to more vulnerable wine consumers.

References

[1] Bauza et al. Food Chemistry, 105 (2007), pp. 405-413.
[2] Anín-Azpilicueta et al. Critical Reviews in Food Science and Nutrition, 48 (3) (2008), pp. 257-275.
[3] Caruso et al., World Journal of Microbiology & Biotechnology, 18 (2002), pp. 159-163.
[4] Konakovsky et al. Food Additives & Contaminants, 28 (4) (2011), pp. 408-416.
[5] Filipe-Ribeiro et al. LWT – Food Science and Technology 115 (2019) pp.108488.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Filipe-Ribeiro Luís1, Milheiro Juliana1, Ferreira Leonor C.1, Correia Elisete2, Cosme Fernanda1 and M. Nunes Fernando

1Chemistry Research Centre-Vila Real (CQ-VR), Food and Wine Chemistry Lab, University of Trás-os-Montes and Alto Douro
2Center for Computational and Stochastic Mathematics (CEMAT), Department of Mathematics, University of Trás-os-Montes and Alto Douro

Contact the author

Keywords

Red wine; Biogenic amines; Dekkera/Brettanomyces; Ethylphenols; Histamine

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Phenological characterization of a wide range of Vitis Vinifera varieties

In order to study the impact of climate change on Bordeaux grape varieties and to assess the adaptation capacities of candidates to the grape varieties of this wine region to the new climatic conditions, an experimental block design composed of 52 grape varieties was set up in 2009 at the INRAE Bordeaux Aquitaine center. Among the many parameters studied, the three main phenological stages of the vine (budburst, flowering and veraison) have been closely monitored since 2012. Observations for each year, stage and variety were carried out on four independent replicates. Precocity indices have been calculated from the data obtained over the 2012-2021 period (Barbeau et al. 1998). This work allowed to group the phenological behaviour of the grapevine varieties, not only based on the timing of the subsequent developmental stages, but also on the overall precocity of the cycle and the total length of the cycle between budburst and veraison. Results regarding the variability observed among the different grape varieties for these phenological stages are presented as heat maps.

Upscaling the integrated terroir zoning through digital soil mapping: a case study in the Designation of Origin Campo de Borja

homogeneous zones by intersecting several partial zonings of major factors that influence vineyard growth. Each of them follows specific process from their corresponding disciplines. Soil zoning specifically refers to a Soil Resource Inventory map that has traditionally been generated by conventional soil mapping methods. These methods have shortcomings in reaching fine cartographic and categorical details and involve significant expenses, which undermines their applicability. A new framework named Digital Soil Mapping has introduced quantitative models by statistical techniques to establish soil-landscape relationships and is able to provide intensive scale cartography.

In the present study, a microzoning at 1:10.000 scale is generated from an initial zoning, where the conventional soil map with polytaxic map units is replaced by a new one from digital techniques that disaggregates them. The comparison between the zonings considers a quantitative evaluation of capability for each Homogeneous Terroir Unit by means of the Viticultural Quality Index and its categorization based on its distribution by map. The spatial intersection of both maps gives rise to a confusion matrix in which the flows of class variations after the substitution are assessed.

The results show a five-fold increase in the number of Homogeneous Terroir Units identified and a larger differentiation among them, evidenced by a wider range in the capability index distribution. Both elements are accompanied by an increase in the detection of areas of higher potential within previously undervalued uniform zones.These features are a direct effect of the improvements brought by Digital Soil Mapping techniques and would verify the advantages of their implementation in the Integrated Terroir zoning. Eventually, such new highly detailed terroir units would benefit precision viticulture and sustainable management practices.

Legacy of land-cover changes on soil erosion and microbiology in Burgundian vineyards

Soils in vineyards are recognized as complex agrosystems whose characteristics reflect complex interactions between natural factors (lithology, climate, slope, biodiversity) and human activities. To date, most of the unknown lies in an incomplete understanding of soil ecosystems, and specifically in the microbial biodiversity even though soil microbiota is involved in many key functions, such as nutrient cycling and carbon sequestration. Soil biological properties are indicative of soil quality. Therefore, understanding how soil communities are related to soil ecosystem functioning is becoming an essential issue for soil strategy conservation. Here, we propose to assess the importance of land-cover history on the present-day microbiological and physico-chemical properties. The studied area was selected in the Burgundian vineyards (Pernand-Vergelesses, Burgundy, France) where land occupation has been reconstructed over the last 40 years. Soil samples were collected in five areas reflecting various land cover history (forest, vineyards, shifting from forest to vineyards). For each area, physico-chemical parameters (pH, C, N, P, grain size) were measured and DNA was extracted to characterize the abundance and diversity of microbial communities. The obtained results show significant differences in the five areas suggesting that present-day microbial molecular biomass and bacterial taxonomic is partly inherited from past land occupation. Over longer period of time, such study of land-uses legacies may help to better assess ecosystem recovery and the impact of management practices for a better soil quality and vineyards sustainability.

Modeling island and coastal vineyards potential in the context of climate change

Climate change impacts regional and local climates, which in turn affects the world’s wine regions. In the short term, these modifications rises issues about maintaining quality and style of wine, and in a longer term about the suitability of grape varieties and the sustainability of traditional wine regions. Thus, adaptation to climate change represents a major challenge for viticulture. In this context, island and coastal vineyards could become coveted areas due to their specific climatic conditions. In regions subject to warming, the proximity of the sea can moderate extremes temperatures, which could be an advantage for wine. However, coastal and island areas are particular prized spaces and subject to multiple pressures that make the establishment or extension of viticulture complex.
In this perspective, it seems relevant to assess the potentialities of coastal and island areas for viticulture. This contribution will present a spatial optimization model that tends to characterize most suitable agroclimatic patterns in historical or emerging vineyards according to different scenarios. Thanks to an in-depth bibliography a global inventory of coastal and insular vineyards on a worldwide scale has been realized. Relevant criteria have been identified to describe the specificities of these vineyards. They are used as input data in the optimization process, which will optimize some objectives and spatial aspects. According to a predefined scenario, the objectives are set in three main categories associated with climatic characteristics, vineyards characteristics and management strategies. At the end of this optimization process, a series of maps presents the different spatial configurations that maximize the scenario objectives.

Geospatial trends of bioclimatic indexes in the topographically complex region of Barolo DOCG

Barolo DOCG is an economically important wine producing region in Northwest Italy. It is a small region of approximately 70 km2 gross area. The topography is very complex with steep sloped hills ranging in elevation from below 200 m to 550 m. Barolo DOCG wine is made exclusively from the Nebbiolo grape. Bioclimatic indexes are often used in viticulture to gain a better understanding of broader climate trends which can be compared temporally and geographically. These indexes are also used for identifying potential phenological timing, growing region suitability, and potential risks associated with expected climatic changes. Understanding how topography influences bioclimatic indexes can help with understanding of mesoscale climate behaviour leading to improved decision making and risk management strategies. The average monthly maximum and minimum temperatures, the Cool Night Index, the Huglin Index, and the monthly diurnal range (from July to October) were calculated using data from 45 weather stations within a 40 km radius of the Barolo DOCG growing area between the years 1996 and 2019. Linear and multiple regression models were developed using independent variables (elevation, aspect, slope) extracted from a digital elevation model to identify significant relationships. Bioclimatic indexes were then kriged with external drift using independent variables that showed significant relationships with the bioclimatic index using a 100 m resolution grid. The maximum monthly temperatures and the Huglin Index showed consistent significant negative relationships with elevation in all years. The minimum monthly temperatures showed no relationship with elevation but in some months a small but significant relationship was observed with aspect. Due to the lack of a relationship between minimum monthly temperatures and elevation compared to the significant relationship between maximum monthly temperatures and elevation, monthly diurnal range had a negative relationship with elevation.