IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Does Dekkera/Brettanomyces wine spoilage raise the risk of biogenic amines intake? A screening in Portuguese red wines

Does Dekkera/Brettanomyces wine spoilage raise the risk of biogenic amines intake? A screening in Portuguese red wines

Abstract

Wine quality and safety are the main concerns of consumers and health agencies. Biogenic amines and polyamines, depending on their concentration and on individuals, in wine can constitute a potential public health concern due to their physiological and toxicological effects. Biogenic amines can be present in grapes, such as putrescine, spermidine, and spermine [1] or formed by microorganisms during the winemaking process such as histamine, cadaverine, hexylamine, and ethylamine [2]. Histamine is one of the targeted toxins by the Food and Drug Administration and the European Food Safety Authority. Dekkera/Brettanomyces, a wine spoilage yeast, can produce biogenic amines in grape juice [3]. Diamines can produce carcinogenic nitrosamines by reaction with nitrite. Biogenic amines are important causes of wine intolerance [4], producing intoxication symptoms.
The sensitivity to biogenic amines depends on insufficient amino oxidase activity, genetic predisposition, alcohol, acetaldehyde, gastrointestinal disease, or inhibition by other amines. Furthermore, it is worth mentioning that, susceptible persons who are immune-compromised and seniors, may exhibit intolerance to even low levels of biogenic amines and suffer more severe symptoms, these persons are increasing in developed countries. Therefore, factors that influence biogenic amines concentrations are of utmost importance for consumer safety, mainly for susceptible persons. The main objective of this study was to quantify biogenic amines and polyamines in industrially produced red wines available in Portuguese wineries. As well as to understand the impact of the spoilage yeasts Dekkera/Brettanomyces in the biogenic amines and polyamines concentrations. Wine sampling was carried out using a maximum variance/heterogeneous purposive non-probability technique. Ethylphenols were determined by GC-MS and biogenic amines and polyamines were determined by dispersive solid-phase extraction and HPLC-DAD after derivatization with benzoyl chloride. To better understand the real input of Dekkera/Brettanomyces activity in these compounds, a set of 79 Portuguese red wines produced at an industrial scale from 2012 to 2016 vintage were analyzed. A total of nine amines have been detected that range from 19.6 to 331 mg/L and concentrations of 4-ethylphenol of 4.5–5604 μg/L and of 4-ethylguaiacol of 2.3–831.2 μg/L [5]. The most abundant amines on average were putrescine followed by histamine and cadaverine. Simultaneous determination of biogenic amines and volatile phenols in industrial produced red wines permitted to conclude that the wine spoilage activity of Dekkera/Brettanomyces with the production of volatile phenols do not significantly contribute to biogenic amines increase and consequently intake by the consumers. Biogenic amines need to be controlled in order to ensure high levels of wine safety and quality to reduce risk to more vulnerable wine consumers.

References

[1] Bauza et al. Food Chemistry, 105 (2007), pp. 405-413.
[2] Anín-Azpilicueta et al. Critical Reviews in Food Science and Nutrition, 48 (3) (2008), pp. 257-275.
[3] Caruso et al., World Journal of Microbiology & Biotechnology, 18 (2002), pp. 159-163.
[4] Konakovsky et al. Food Additives & Contaminants, 28 (4) (2011), pp. 408-416.
[5] Filipe-Ribeiro et al. LWT – Food Science and Technology 115 (2019) pp.108488.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Filipe-Ribeiro Luís1, Milheiro Juliana1, Ferreira Leonor C.1, Correia Elisete2, Cosme Fernanda1 and M. Nunes Fernando

1Chemistry Research Centre-Vila Real (CQ-VR), Food and Wine Chemistry Lab, University of Trás-os-Montes and Alto Douro
2Center for Computational and Stochastic Mathematics (CEMAT), Department of Mathematics, University of Trás-os-Montes and Alto Douro

Contact the author

Keywords

Red wine; Biogenic amines; Dekkera/Brettanomyces; Ethylphenols; Histamine

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.

Terroir traceability in grapes, musts and wine: results of research on Gewürztraminer and Sauvignon Blanc grape varieties in northern Italy

In the study of terroir, a separate analysis of its many component factors can be of great help in accurately identifying a vineyard’s natural elements that impact wine quality and typicity. This research used a dedicated pluri-disciplinary approach to investigate the ecological characteristics, including geology and geographical features, of 14 vineyards that produce Gewürztraminer and Sauvignon Blanc cultivars in the alpine Alto Adige DOC wine region. Both the geopedological method using Vineyards Geological Identity (VGI) and the new Solar Radiaton Identity (SRI) topoclimatic classification method were used to provide analytical measurements and qualitative/quantitative characterisations. In addition, wide-ranging targeted and untargeted oenological and chemical analyses were carried out on grapes, musts and wines to correlate the soils’ geomineral and physical conditions with the biochemical properties of their fruits and wines. The research identified strong correlations between vineyard geo-identity and wine biofingerprint, confirming a mineral traceability of strontium rubidium ratio and some minerals distinctive to the local geology, such as K, Ca, Ag, Ba and Mn.  The study also discovered that particular geomineral and physical soil conditions of the studied vineyards are related to the different amount of amino acids, primary varietal aromas and polyphenols found in grapes, musts and wines. The research confirmed that winemaking technologies support oenological quality, although in some cases, human practices can overpower certain characteristic elements in wine, erasing the typical imprint left by the vineyards’ natural terroir, which becomes less traceable. Terroir abiotic ecological factors and vineyard identity can be classified in detail using the new VGI and SRI analysis methods to discover interrelationships between geo-pedological and topoclimatic conditions that impact wine quality. These methods are also helpful in identifying which ecological elements are exclusive to a particular vineyard or wine sub-region.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…