IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Structural composition of polymeric polyphenols of red wine after long-term ageing: effect of vinification technology

Structural composition of polymeric polyphenols of red wine after long-term ageing: effect of vinification technology

Abstract

Aged red wines possess phenolic composition very different from young ones due to the transformations among native grape phenolics and the formation of new polymeric polyphenols during aging process. In this work, Syrah red wines were made by different winemaking technologies, i.e., traditional fermentation on skin (total 7 days of maceration), prolonged maceration with addition of extra skins at the end of traditional fermentation (total 14 days of maceration) and prolonged maceration with addition of extra stems at the end of traditional fermentation (total 14 days of maceration). After 8 years of ageing in bottle, the structural composition of polymeric polyphenols in these wines was comprehensively analysed through different degradation methods (hydrochloric acid hydrolysis, NaOH hydrolysis and Benzyl mercaptan hydrolysis), followed by HPLC-FT-ICR-MS, HPLC/UPLC-MS analysis. The results showed that the molecules of polymeric polyphenols in the aged red wines were composed of not only proanthocyanidins but also anthocyanins, amino acids and phenolic acids. The percentages of the constitutive units of the polymeric polyphenol molecules in these wines varied considerably, being catechin (7.1 – 14.9%), epicatechin (74.5 – 78.2%), epicatechin-3-O-gallate (5.8 – 12.2%), amino acids (0.7 – 1.5%), phenolic acids (0.0 – 0.9%) anthocyanins (0.1 – 0.4%) and epigallocatechin (0.7 – 4.7%),  depending on the type of the winemaking technologies. Catechin, epicatechin and epicatechin-3-O-gallate were presented as both terminal and extension units, with the latter predominant, while amino acids, phenolic acids and anthocyanins were found to be presented exclusively as terminal units and epigallocatechin was found to be presented exclusively as extension units. Comparing with the wine made by traditional fermentation on skin, the lower phenolic acids and anthocyanins units was found in the wine made by prolonged fermentation/maceration with skin and with stem. The prolonged fermentation/maceration with skin was found to have highest amino acids units. On the other hand, different vinification technologies affected the mean polymerization degrees (mDP) of polymeric polyphenols in the aged red wines, being mDP 25.2 for the control one, mDP 13.1 for the wine made by the prolonged fermentation with skin and mDP 15.7 for the prolonged fermention with stem. These results indicated that, different winemaking technologies affect significantly the structural features of polymeric polyphenols.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Sun Baoshan1, Jian Zhao3, Tingting Yang1, Martins Patrícia2, Ramos João4 and Lingxi Li1

1School of Functional Food and Wine, Shenyang Pharmaceutical University
2Pólo Dois Portos, Instituto Nacional de Investigação Agrária e Veterinária, I.P.

3School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University
4Departamento de Enologia, Herdade do Esporão, Reguengos de Monsaraz

Contact the author

Keywords

polymeric polyphenols; winemaking technology; structural composition; aged red wine

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).

Grapevine yield-gap: identification of environmental limitations by soil and climate zoning in Languedoc-Roussillon region (south of France)

Grapevine yield has been historically overlooked, assuming a strong trade-off between grape yield and wine quality. At present, menaced by climate change, many vineyards in Southern France are far from the quality label threshold, becoming grapevine yield-gaps a major subject of concern. Although yield-gaps are well studied in arable crops, we know very little about grapevine yield-gaps. In the present study, we analysed the environmental component of grapevine yield-gaps linked to climate and soil resources in the Languedoc Roussillon. We used SAFRAN data and IGP Pays d’Oc wine yields from 2010 to 2018. We selected climate and soil indicators proving to have a significant effect on average wine yield-gaps at the municipality scale. The most significant factors of grapevine yield were the Soil Available Water Capacity; followed by the Huglin Index and the Climatic Dryness Index. The Days of Frost; the Soil pH; and the Very Hot Days were also significant. Then, we clustered geographical zones presenting similar indicators, facilitating the identification of resources yield-gaps. We discussed the number of zones with the experts of IGP Pays d’Oc label, obtaining 7 zones with similar limitations for grapevine yield. Finally, we analysed the main resources causing yield-gaps and the grapevine varieties planted on each zone. Mapping grapevine resource yield-gaps are the first stage for understanding grapevine yield-gaps at the regional scale.

Towards a regional mapping of vine water status based on crowdsourcing observations

Monitoring vine water status is a major challenge for vineyard management because it influences both yield and harvest quality. It is also a challenge at the territorial scale for identifying periods of high water restriction or zones regularly impacted by water stress. This information is of major importance for defining collective strategies, anticipating harvest logistic or applying for irrigation authorisation. At this spatial scale, existing tools and methods for monitoring vine water status are few and often require strong assumptions (e.g. water balance model). This paper proposes to consider a collaborative collection of observations by winegrowers and wine industry stakeholders (crowdsourcing) as an interesting alternative. Indeed, it allows the collection of a large number of field observations while pooling the collection effort. However, the feasibility of such a project and its interest in monitoring vine water status at regional scale has never been tested.

The objective of this article is to explore the possibility of making a regional map of vine water status based on crowdsourcing observations. It is based on the study of the free mobile application ApeX-Vigne, which allows the collection of observations about vine shoot growth. This information is easy to collect and can be considered, under certain conditions, as a proxy for vine water status. This article presents the first results obtained from the nearly 18,000 observations collected by winegrowers and wine industry stakeholders during 2019, 2020 and 2021 seasons. It presents the vine shoot growth maps obtained at regional scale and their evolution over the three vintages studied. It also proposes an analysis of the factors that favoured the number of observations collected and those that favoured their quality. These results open up new perspectives for monitoring vine water status at a regional scale but above they provide references for other crowdsourcing projects in viticulture.