terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of spray with autochthonous Trichoderma strains and its secondary metabolites on the quality of Tempranillo grape

Effect of spray with autochthonous Trichoderma strains and its secondary metabolites on the quality of Tempranillo grape


Trichoderma is one of the most widely used fungal biocontrol agents on vineyards due to its multiple benefits on this crop, such as its fungicidal and growth promoting capacity. In this work, we have analyzed the effect on the concentration of nutrients in grapevine leaves and on the quality of the grape must after spraying an autochthonous strain of Trichoderma harzianum and one of the main secondary metabolites produced by this genus, 6-pentyl-α-pyrone (6PP).

The trials were carried out in two regular vine plantations (Vitis vinifera cv. Tempranillo). The experimental design was in randomized blocks with three treatments (Trichoderma harzianum autochthonous strain, 6PP and control) and 10 repetitions (plants) per block. A total of 6 applications of treatments were made on the entire vegetative part of the plant every 15 days. During the development of the test, levels of potassium, calcium and magnesium were measured in the vine leaves and must quality parameters were analyzed at the time of harvest.

The nutrient data showed an increase in the concentration of potassium in leaves in the treatment with the autochthonous Trichoderma harzianum strain compared to the other two treatments. In the case of the analysis of the quality of the grapes, an increase in the concentrations of malic acid, total acidity, potassium, probable º and density of the must was also observed in the treatment with the autochthonous Trichodermacompared to the rest of the treatments.

Thanks to the Ministry of Education, Culture and Sports (Spain) for the grant awarded to Laura Zanfaño González (FPU 20/03040), to the own research program of the University of León 2022 for the grant awarded to Daniela Ramírez Lozano. As well as the project acronym LOWPHWINE, reference IDI-20210391.


Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster


Zanfaño L.1*, Carro-Huerga G.1, Mayo-Prieto S.1, Rodríguez-González A.1, Ramírez-Lozano D.1, Gutiérrez S.2, Casquero P.A.1

1Grupo de Investigación de Ingeniería y Agricultura Sostenible (GUIIAS). Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de León Av. Portugal 41, 24071 León, España.
2Grupo Universitario de Investigación en Ingeniería y Agricultura Sostenible (GUIIAS). Área de Microbiología. Escuela de Ingeniería Agraria y Forestal. Campus de Ponferrada. Universidad de León. León. España.

Contact the author*


Trichoderma harzianum, 6PP, potassium, quality of the grapes


2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series


Related articles…

Association between dietary pattern and wine consumption and Alzheimer’s disease in a cohort from La Rioja (Spain)

Addressing modifiable risk factors is the most promising strategy to prevent/delay Alzheimer Disease (AD)[1]. The aim of the study was to establish the connections between dietetic habits, wine consumption and AD. Thus, 98 volunteers were recruited: 50 diagnosed as AD and 48 healthy/controls. The Food Frequency Questionnaire (FFQ) was used for dietary patterns assessment and, based on these data, the Mind Diet Score was calculated. (Poly)phenol metabolites (especially derived from wine consumption) were analyzed by UPLC-QqQ-MS/MS in 24-h urine samples to confirm dietary (poly)phenol consumption.

The generation of suspended cell wall material may limit the effect of ultrasound in some varieties

The disruptive effect exerted by high-power ultrasound (US) on plant cell walls, natural barriers to the diffusion of compounds of interest during the maceration of red wines, is established as the reason behind the chromatic improvement that its treatment causes. However, sometimes this improvement is not observed, especially with short maceration times. The presence of a high quantity of suspended cell wall material, which formation is favored by the sonication, could be the cause of this lack of positive results since this cell wall material has a high affinity for phenolic compounds.

Climate change and viticulture in Nordic Countries and the Helsinki area

The first vineyards in Northern Europe were in Denmark in the 15th century, in the southern parts of Sweden and Finland in the 18th century at 55–60 degrees latitude. The grapes grown there have not been made into wine, but the grapes have been eaten at festive tables. The resurgence of viticulture has started with global warming, and currently the total area of viticulture in the Nordic countries, including Norway, is estimated to be 400–500 hectares, most of which is in Denmark. Southern Finland, like all southern parts of Northern Europe, belongs to the cool-cold winegrowing area.

Barrels ad-hoc: Spanish oak wood classification by NIRs 

The wooden barrel is a key factor in enology, since wine chemical composition and sensory properties changes significantly in contact with the barrel[1]. Today’s highly competitive market constantly demands new differentiated products and wineries search innovations continuously.
Wood selection is crucial: barrels stability to keep constant their contribution and the result on products, and additional and differentiated wood contributions to impact their new products. Oak wood selection has traditionally been carried out using parameters such as specie, location and grain, however, it goes one step further nowadays. Large cooperage work with non-destructive techniques that allow classifying oak wood quickly and easily according to their organoleptic contribution[2].

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.