Development of analytical sampling technique to study the aroma profile of Pinot Noir wine

Downy mildew is one of the most important grapevine diseases, caused by the Oomycete Plasmopara viticola. The management of the disease in organic agriculture can require up to 15 copper applications per year. However, copper accumulates in the soil, is phytotoxic and is toxic for organisms living in the soil, its use has been restricted in European Union to maximum 28 kg in 7 years. Therefore, testing of alternatives with equal effectiveness is desirable. Among those, the natural biopolymer chitosan, obtained from crab shells, proved to be effective toward downy mildew in plot experiments. The aim of our trials was to extend chitosan applications in large scale experiments in different years, cultivars and environmental conditions.

Climate change is having an unprecedented impact on the wine industry, which is one of the major agricultural sectors around the world. Global warming, combined with the variation in rainfall patterns and the increase in frequency of extreme weather events, is significantly influencing vine physiology and exposing, more frequently, plants to severe biotic and abiotic stresses. This represents a challenge for viticulturists who need to take complex decisions to adjust vineyard management and achieve oenological goals.

Polysaccharides (PS) are one of the main compounds found in wines, and they come mainly from the grape cell walls or from the yeasts, and they play an important role in the technological and sensory characteristics of wines. Polysaccharides obtained from yeasts have been more studied, especially mannoproteins, since there are commercial products.

A terroir can be defined as a natural unit that is characterised by a specific agricultural potential, which is imparted by natural environmental features, and is reflected in the characteristics of the final product.

Wine lees are the second most important wine by-product in terms of quantity after grape stalk and marc. During aging on lees, it is well known that wine lees yield compounds that act as antioxydant. However the chemical nature of the compounds involved in this behavior (except polyphenols and glutathione) has not yet been totally elucidated. The scarce knowledge of wine lees composition and their potential exploitation make them a promising candidate to obtain new antioxidant products to be used in winemaking. In this study, an eco-sustainable approach to obtain lees extracts exhibiting antioxidant capacity is proposed. Such extracts could be used in a global enological strategy of sulfites level reduction.