IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Development of analytical sampling technique to study the aroma profile of Pinot Noir wine

Development of analytical sampling technique to study the aroma profile of Pinot Noir wine

Abstract

A novel and efficient Dispersive Liquid-Liquid Microextraction (DLLME) method coupled with gas chromatography–mass spectrometry (GC–MS) was developed to determine 33 key aroma compounds (esters, alcohols, aldehydes, terpenes, norisoprenoids, fatty acids and phenols) present in Pinot noir (PN) wine. Four critical parameters including extraction solvent type, disperse solvent type, extraction solvent volume and disperse solvent volume were optimised with the aid of D-optimal design. Linearity of standard calibration curves created with the optimised method was satisfactory (with correlation coefficients over 0.9917), and repeatability and reproducibility were better than 10% for all targeted analytes. The limits of detection and the limits of quantification were at very low levels (µg L-1), covering the range of expected concentrations for targeted compounds in PN wine. Finally, the developed method was successfully applied to analyse 12 New Zealand PN wines. To our knowledge, this is the first time DLLME has been applied simultaneously to determine all the above aroma compounds present in PN wine. The developed DLLME method is a fast, straight-forward and low-cost method that is more environmentally-friendly than other common volatile extraction methods. 

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Katugampala Appuhamilage Dinesha Hansamali Perera1, Fedrizzi Bruno1, Pilkington Lisa Ivy1, Jelley Rebecca Eleanor1, Sherman Emma2 and Pinu Farhana R.2

1University of Auckland
2Plant and Food Research, New Zealand

Contact the author

Keywords

Wine, Dispersive liquid–liquid microextraction (DLLME), D-optimal design, Gas chromatography–mass spectrometry, aroma compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Impact of mycorrhizal inoculation of ‘Monastrell’ grapevines grafted onto different conventional vs. newly breed rootstocks 

Grafting Vitis vinifera L. (wine traditional cultivars) onto North American grapevine species or hybrids is a common practice in viticulture given their tolerance against phylloxera (Daktulosphaira vitifoliae). However, rootstock genetic background affects the response of grapevines to environmental stresses and their ability for establishing a symbiotic relationship with the microbial communities, and more specifically with arbuscular mycorrhizal fungi (AMF). The aim of this study was to evaluate Monastrell variety (clone ENTAV 369) grafted onto three rootstocks (140Ru, 110R and RG8) characterized by a different genetic background, in combination with AMF inoculation (Rhizophagus irregularis) vs. a non-inoculated control with regards to vegetative growth, leaf gas exchange parameters, and mycorrhization.
Read More

CropManage online decision support tool for irrigation scheduling of vineyards

CropManage (CM) is an online decision support service (DSS) developed by the University of California, Division of Agriculture and Natural Resources for assisting farmers with efficiently managing water and nitrogen fertilizer to match the site-specific needs of their crops.

Read More

Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

The interactions among aromatic compounds and proteins is an important issue for the quality of foods and beverages. In wine, the loss of flavor after vinification is associated to bentonite treatment and this effect can be the result of the removal of aroma compounds which are bound wine proteins. This phenomenon was recently demonstrated for long chain fatty acids and their ethyl esters (1). Since these latter compounds are spectroscopically silent, their association with proteins is not easy to measure.

Read More

Assessing and mapping vineyard water status variability using a miniaturized nir spectrophotometer from a moving vehicle

In the actual scenario of climate change, optimization of water usage is becoming critical in sustainable viticulture. Most of the current approaches to assess grapevine water status and drive irrigation scheduling are either destructive, time and labour consuming and monitor a small, limited number of plants. This work presents a novel methodology using a contactless, miniaturized, low-cost NIR spectrometer to monitor the vineyard water status variability from a moving vehicle, to provide reliable information towards precision irrigation.

Read More

Différenciation de parcelles de Chenin du Val de Loire, a l’aide de l’etude des flores fongiques des raisins, en utilisant l’outil DGGE

Depuis le millésime 2002, une étude est menée sur la diversité de la flore fongique de parcelles du cépage chenin, situées essentiellement sur les appellations de Vouvray et Montlouis ; deux appellations séparées par le fleuve nommé la Loire. Les parcelles se situent dans des conditions pédoclimatiques différentes, qui se retrouvent au travers des suivis de maturité et l’état sanitaire.

Read More