IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Development of analytical sampling technique to study the aroma profile of Pinot Noir wine

Development of analytical sampling technique to study the aroma profile of Pinot Noir wine

Abstract

A novel and efficient Dispersive Liquid-Liquid Microextraction (DLLME) method coupled with gas chromatography–mass spectrometry (GC–MS) was developed to determine 33 key aroma compounds (esters, alcohols, aldehydes, terpenes, norisoprenoids, fatty acids and phenols) present in Pinot noir (PN) wine. Four critical parameters including extraction solvent type, disperse solvent type, extraction solvent volume and disperse solvent volume were optimised with the aid of D-optimal design. Linearity of standard calibration curves created with the optimised method was satisfactory (with correlation coefficients over 0.9917), and repeatability and reproducibility were better than 10% for all targeted analytes. The limits of detection and the limits of quantification were at very low levels (µg L-1), covering the range of expected concentrations for targeted compounds in PN wine. Finally, the developed method was successfully applied to analyse 12 New Zealand PN wines. To our knowledge, this is the first time DLLME has been applied simultaneously to determine all the above aroma compounds present in PN wine. The developed DLLME method is a fast, straight-forward and low-cost method that is more environmentally-friendly than other common volatile extraction methods. 

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Katugampala Appuhamilage Dinesha Hansamali Perera1, Fedrizzi Bruno1, Pilkington Lisa Ivy1, Jelley Rebecca Eleanor1, Sherman Emma2 and Pinu Farhana R.2

1University of Auckland
2Plant and Food Research, New Zealand

Contact the author

Keywords

Wine, Dispersive liquid–liquid microextraction (DLLME), D-optimal design, Gas chromatography–mass spectrometry, aroma compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

INFLUENCE OF CHITOSAN, ABSCISIC ACID AND BENZOTHIADIAZOLE TREATMENTS ON SAVVATIANO (VITIS VINIFERA L.) WINES VOLATILE COMPOSITION PROFILE

In the last decades the use of bioestimulants in viticulture have been promoted as alternative to conven- tional pesticides. Moreover, as bioestimulants promote the biosynthesis of secondary metabolites in grape berries, several studies had investigated their influence on the accumulation of phenolic com- pounds (Monteiro et al., 2022). However, few studies, so far, are focused on the accumulation of the vo- latile compounds and their impact on the produced wines (Giménez-Bañón et al., 2022; Gomez- Plaza et al., 2012; Ruiz Garcia et al., 2014). This study was conducted in a single vineyard of white autochthonous grapevine variety Savvatia- no (Vitis vinifera L.) in Muses Valley (Askri, Viotia, Greece). Chitosan (CHT), Abscisic Acid (ABA) and Benzothiadiazole (BTH) were applied.

Impact assessment of the reverse osmosis technique in wine alcohol management

Wine authenticity and composition can be influenced by a range of membrane separation processes as reverse osmosis. In the context of climate change, the natural trend is to obtain wines with higher alcoholic concentration when classical winemaking methods are employed, and this may induce alteration of typicity of wines by masking the olfactory and taste properties. This study aimed to evaluate the influence of reverse osmosis techniques used for decrease of ethanol content on the stable isotopic ratios as markers for wine authenticity characteristics.

Response of different nitrogen supplementation on Saccharomyces cerevisiae metabolic response and wine aromatic profile

The wine yeast Saccharomyces cerevisiae can highly affect wine aromatic profile by producing and/or mediating the release of a whole range of metabolites (such as thiols, esters, and terpenes), which in turn contribute to enhanced aroma and flavor. These metabolites depend on yeast metabolism activated during fermentation which can constitute the ‘’metabolic footprint’’ of the yeast strain that carried out the process.

Viticultural climate effect on the sensorial perception of wines. Methodological elements for a modelling at a world level

The objective of this study was to develop a methodology capable of modeling the effect of viticultural climate on wine sensory characteristics.

Cell Walls Of Grape Mesocarp Possible Fining Agents For Red And White Wine

Clarification or fining of wines is a technique used in wineries to eliminate unwanted wine components, which negatively affect its quality. Clarification normally involves the addition of an adsorptive material that eliminates or reduces the presence of undesirable components. The problem is that many of the fining agents used in the industry contain allergens, such as caseinates or ovalbumin.