IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Development of analytical sampling technique to study the aroma profile of Pinot Noir wine

Development of analytical sampling technique to study the aroma profile of Pinot Noir wine

Abstract

A novel and efficient Dispersive Liquid-Liquid Microextraction (DLLME) method coupled with gas chromatography–mass spectrometry (GC–MS) was developed to determine 33 key aroma compounds (esters, alcohols, aldehydes, terpenes, norisoprenoids, fatty acids and phenols) present in Pinot noir (PN) wine. Four critical parameters including extraction solvent type, disperse solvent type, extraction solvent volume and disperse solvent volume were optimised with the aid of D-optimal design. Linearity of standard calibration curves created with the optimised method was satisfactory (with correlation coefficients over 0.9917), and repeatability and reproducibility were better than 10% for all targeted analytes. The limits of detection and the limits of quantification were at very low levels (µg L-1), covering the range of expected concentrations for targeted compounds in PN wine. Finally, the developed method was successfully applied to analyse 12 New Zealand PN wines. To our knowledge, this is the first time DLLME has been applied simultaneously to determine all the above aroma compounds present in PN wine. The developed DLLME method is a fast, straight-forward and low-cost method that is more environmentally-friendly than other common volatile extraction methods. 

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Katugampala Appuhamilage Dinesha Hansamali Perera1, Fedrizzi Bruno1, Pilkington Lisa Ivy1, Jelley Rebecca Eleanor1, Sherman Emma2 and Pinu Farhana R.2

1University of Auckland
2Plant and Food Research, New Zealand

Contact the author

Keywords

Wine, Dispersive liquid–liquid microextraction (DLLME), D-optimal design, Gas chromatography–mass spectrometry, aroma compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Ampelograpic and genetic characterisation of grapevine genetic resources from Ozalj-Vivodina region (Croatia)

Ozalj- vivodina region is small vine growing area (only about 100 hectares of vineyards), but with significant number of old, ancient vineyards planted between 50 and 100 years ago. Trend of abandoning or replanting ancient vineyards takes place for the last 30 years. This trend results in grapevine germplasm erosion because traditional varieties are replaced with well known international varieties.Few known traditional varieties are dominantly present in ancient vineyards together with many others of unknown identity. Historical data about prevalence and characteristic of varieties on this area are very poor.

Modelling vine water stress during a critical period and potential yield reduction rate in European wine regions: a retrospective analysis

Most European vineyards are managed under rainfed conditions, where seasonal water deficit has become increasingly important. The flowering-veraison phenophase represents an important period for vine response to water stress, which is seldomly thoroughly evaluated. Therefore, we aim to quantify the flowering-veraison water stress levels using Crop Water Stress Indicator (CWSI) over 1986–2015 for important European wine regions, and to assess the respective potential Yield Lose Rate (YLR). Additionally, we also investigate whether an advanced flowering-veraison phase may help alleviating the water stress with improved yield. A process-based grapevine model STICS is employed, which has been extensively calibrated for flowering and veraison stages using observed data at 38 locations with 10 different grapevine varieties. Subsequently, the model is being implemented at the regional level, considering site-specific calibration results and gridded climate and soil datasets. The findings suggest wine regions with stronger flowering-veraison CWSI tend to have higher potential YLR. However, contrasting patterns are found between wine regions in France-Germany-Luxembourg and Italy-Portugal-Spain. The former tends to have slight-to-moderate drought conditions (CWSI<0.5) and a negligible-to-moderate YLR (<30%), whereas the latter possesses severe-to-extreme CWSI (>0.5) and substantial YLR (>40%). Wine regions prone to a high drought risk (CWSI>0.75) are also identified, which are concentrated in southern Mediterranean Europe. An advanced flowering-veraison phase may have benefited from cooler temperatures and a higher fraction of spring precipitation in wine regions of Italy-Portugal-Spain, resulting in alleviated CWSI and moderate reductions of YLR. For those of France-Germany-Luxembourg, this can have reduced flowering-veraison precipitation, but prevalent alleviations of YLR are also found, possibly because of shifted phase towards a cooler growing season with reduced evaporative demands. Overall, such a retrospective analysis might provide new insights towards better management of seasonal water deficit for conventionally vulnerable Mediterranean wine regions, but also for relatively cooler and wetter Central European regions.

Sviluppo di una metodologia di tracciabilità e definizione dell’impronta petrochimica in suoli e vini della Sicilia occidentale nella piana di Marsala (TP)

I risultati delle ricerche condotte in un vigneto sperimentale di Marsala (TP), scelto per omogeneità di fattori bio-agronomici (età, tecniche colturali, potenzialità vegetativa e produttiva)

The influence of external factors on the alcoholic fermentation of wine yeasts

Wine yeast strains Saccharomyces ellipsoideus have important applications in food industry and in this regard is sought isolation as pure cultures and selecting those strains

Carry over effect of shoot trimming and deficit irrigation on fruit yield and berry total soluble solids

The increase in air temperature that is occurring in many important wine-growing areas around the world is resulting in the decoupling between the phenolic and the technological maturity of grapevine berries. This new ripening pattern leads to the production of light-bodied high alcoholic wines, but this is in countertendency with the increasing consumers’ demand for wines with low-to-mid alcohol concentrations. The oenological techniques proposed to reduce wine alcohol content are often very expensive and lead to detrimental effects on wine quality. Many viticultural practices have been proposed to slow down sugar accumulation the berry. One possible strategy that was previously found to be suitable for Aglianico grapevine is post-veraison shoot trimming. The aim of this work was to assess the carry over effects on the following year of shoot trimming and vine water status on yield and total soluble solids because the expected reduction in vine fertility could lead to a reduction in the effectiveness of shoot trimming.