IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Phenolic compounds of wine spirits resulting from different ageing technologies: behaviour during the storage in bottle

Phenolic compounds of wine spirits resulting from different ageing technologies: behaviour during the storage in bottle

Abstract

Phenolic compounds are released from the wood into the wine spirit (WS) during the ageing process, and are of utmost importance to the colour, flavour, taste and the overall quality acquired by this spirit drink.1 Their concentrations in the WS and the related effects mainly depend on the kind of wood (oaks vs chestnut), toasting level and ageing technology (traditional using wooden barrels vs alternative).1,2,3

Recent research conducted by our team has been focused on alternative technology towards sustainable ageing of WS resorting to wooden staves combined with micro-oxygenation (MOX).2,3 In the Project CENTRO-04-3928-FEDER-000001, the same wine distillate was aged for 18 months in 1000 L stainless steel tanks with wood staves inside (Limousin oak or chestnut) and MOX (flow rate 2 mL/L/month), and in 250 L barrels made of the same kinds of wood, in duplicate. Despite the promising results achieved, showing faster ageing and the production of high quality aged WSs resulting from the alternative technology compared to those resulting from the traditional one, it is imperative to assess their quality during the storage in bottle to fully validate the new technology. Therefore, the second phase of the investigation is currently made under the Project CENTRO-04-3928-FEDER-000028, studying the chemistry underlying the storage in bottle in order to understand if the features imparted by the ageing technology are retained or if they do not persist. The present work is focused on the behaviour of phenolic compounds of the aged WSs during this stage. For this purpose, the eight aged WSs (from the four modalities: chestnut barrels, Limousin oak barrels, stainless steel tanks with chestnut wood staves and MOX, and stainless steel tanks with Limousin oak wood staves and MOX) were bottled on the same day in 750 mL amber glass bottles (two bottles per modality). The cork stopper and the bottleneck were sealed with parafilm to prevent evaporation. The bottles were stored in the cellar of INIAV-Dois Portos. Sampling was carried out in the beginning and after 12 months of storage, and the phenolic compounds (gallic, syringic, ferulic and ellagic acids, vanillin, syringaldehyde, coniferaldehyde, sinapaldehyde, umbelliferone and scopoletin) were analysed by a HPLC method developed and validated in our laboratory.4

The ANOVA results revealed that most of the compounds’ contents did not change significantly after 12 months of storage in bottle. In addition, the phenolic differences between the WSs resulting from the four ageing modalities remained, except for ferulic acid. Therefore, in these experimental conditions, this stage allowed preserving the phenolic composition imparted to the WS by the alternative ageing technology.

References

1 Canas S., 2017. Phenolic composition and related properties of aged wine spirits: Influence of barrel characteristics. A review. Beverages, 3, 55-76.
2 Canas S., Anjos O., Caldeira I., Belchior A.P., 2019. Phenolic profile and colour acquired by the wine spirit in the beginning of ageing: alternative technology using micro-oxygenation vs traditional technology. LWT – Food Science and Technology, 111, 260-269.
3 Granja-Soares J., Roque R., Cabrita M.J., Anjos O., Belchior A.P., Caldeira I., Canas S., 2020. Effect of innovative technology using staves and micro-oxygenation on the sensory and odorant profile of aged wine spirit. Food Chem., 333, 127450.
4 Canas S., Belchior A.P., Spranger M.I., Bruno de Sousa R., 2003. High-performance liquid chromatography method for analysis of phenolic acids, phenolic aldehydes and furanic derivatives in brandies. Development and validation. J. Sep. Sci., 26, 496–502.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Canas Sara1, Lourenço Sílvia1, Anjos Ofélia2 and Caldeira Ilda1

1Instituto Nacional de Investigação Agrária e Veterinária – Pólo de Dois Portos
2 Instituto Politécnico de Castelo Branco 

Contact the author

Keywords

wine spirit, storage in bottle, ageing technology, phenolic compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Nitrogen partitioning among vine organs as a consequence of cluster thinning

Agroscope is investigating the impact of yield on nitrogen (N) partitioning in grapevine and on must composition. The mechanism of N assimilation

La perception des terroirs du vignoble des Coteaux du Layon

On peut être surpris de l’existence d’un vignoble de vins liquoreux, le vignoble des Coteaux du Layon, dans une zone septentrionale à la limite Nord de la culture de qualité de la vigne et ce d’autant plus que le cépage de ce vignoble, le Chenin ou Pineau de la Loire, est un cépage semi tardif. La première explication est à rechercher au niveau des facteurs naturels (données climatiques et géopédologiques) permettant la réalisation de ce type de produit. Il est nécessaire de souligner ici l’importance de chaque paramètre du terroir pris dans im sens large (géopédologique et climatique) et que toute variation de l’un d’entre eux, même non perceptible en première analyse à l’homme, peut avoir des incidences déterminantes sur la qualité des vins.

Effect of terroir and winemaking protocol on the chemical and sensory profiles of Pinot Blanc wine

Wine research in the past years has mainly been focused on laboratory scale due to the possibility of controlling winemaking variables. Conversely, studies on wine quality in relation to the winemaking variables at the winery scale may be able to better account for the actual challenges encountered during wine production. Winemaking problems are recently arising from progressive changes in environmental conditions in relation to the terroir. It is important to realize that each wine region may have specific winemaking protocols and that winemakers often base their decisions on subjective, emotional, and empirical opinions. Due to all the above-mentioned issues, taking the correct decision in winemaking to achieve the desired goals may become even more challenging.

YEAST-PRODUCED VOLATILES IN GRAPE BASED SYSTEM MODEL ACTING AS ANTIFUNGAL BIOAGENTS AGAINST PHYTOPATHOGEN BOTRYTIS CINEREA

Botrytis cinerea Pers., the causal agent of grey mould disease, is responsible for substantial economic losses, as it causes reduction of grape and wine quality and quantity. Exploitation of antagonistic yeasts is a promising strategy for controlling grey mould incidence and limiting the usage of synthetic fungicides. In our previous studies, 119 different indigenous yeasts were screened for putative multidimensional modes of action against filamentous fungus B. cinerea [1]. The most promissing biocontrol yeast was Pichia guilliermondii ZIM624, which exhibited several anatagonistic traits (production of cell wall degrading enzymes, chitinase and β-1,3-glucanase; demonstration of in vitro inhibitory effect on B. cinerea mycelia radial growth; production of antifungal volatiles, assimilation of a broad diversity of carbon sources, contributing to its competitivnes in inhabiting grapes in nature).

Impact of yeast derivatives to increase the phenolic maturity and aroma intensity of wine

Using viticultural and enological techniques to increase aromatics in white wine is a prized yet challenging technique for commercial wine producers. Equally difficult are challenges encountered in hastening phenolic maturity and thereby increasing color intensity in red wines. The ability to alter organoleptic and visual properties of wines plays a decisive role in vintages in which grapes are not able to reach full maturity, which is seen increasingly more often as a result of climate change. A new, yeast-based product on the viticultural market may give the opportunity to increase sensory properties of finished wines. Manufacturer packaging claims these yeast derivatives intensify wine aromas of white grape varieties, as well as improve phenolic ripeness of red varieties, but the effects of this application have been little researched until now. The current study applied the yeast derivative, according to the manufacture’s instructions, to the leaves of both neutral and aromatic white wine varieties, as well as on structured red wine varieties. Chemical parameters and volatile aromatics were analyzed in grape musts and finished wines, and all wines were subjected to sensory analysis by a tasting panel. Collective results of all analyses showed that the application of the yeast derivative in the vineyard showed no effect across all varieties examined, and did not intensify white wine aromatics, nor improve phenolic ripeness and color intensity in red wine.