IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Phenolic compounds of wine spirits resulting from different ageing technologies: behaviour during the storage in bottle

Phenolic compounds of wine spirits resulting from different ageing technologies: behaviour during the storage in bottle

Abstract

Phenolic compounds are released from the wood into the wine spirit (WS) during the ageing process, and are of utmost importance to the colour, flavour, taste and the overall quality acquired by this spirit drink.1 Their concentrations in the WS and the related effects mainly depend on the kind of wood (oaks vs chestnut), toasting level and ageing technology (traditional using wooden barrels vs alternative).1,2,3

Recent research conducted by our team has been focused on alternative technology towards sustainable ageing of WS resorting to wooden staves combined with micro-oxygenation (MOX).2,3 In the Project CENTRO-04-3928-FEDER-000001, the same wine distillate was aged for 18 months in 1000 L stainless steel tanks with wood staves inside (Limousin oak or chestnut) and MOX (flow rate 2 mL/L/month), and in 250 L barrels made of the same kinds of wood, in duplicate. Despite the promising results achieved, showing faster ageing and the production of high quality aged WSs resulting from the alternative technology compared to those resulting from the traditional one, it is imperative to assess their quality during the storage in bottle to fully validate the new technology. Therefore, the second phase of the investigation is currently made under the Project CENTRO-04-3928-FEDER-000028, studying the chemistry underlying the storage in bottle in order to understand if the features imparted by the ageing technology are retained or if they do not persist. The present work is focused on the behaviour of phenolic compounds of the aged WSs during this stage. For this purpose, the eight aged WSs (from the four modalities: chestnut barrels, Limousin oak barrels, stainless steel tanks with chestnut wood staves and MOX, and stainless steel tanks with Limousin oak wood staves and MOX) were bottled on the same day in 750 mL amber glass bottles (two bottles per modality). The cork stopper and the bottleneck were sealed with parafilm to prevent evaporation. The bottles were stored in the cellar of INIAV-Dois Portos. Sampling was carried out in the beginning and after 12 months of storage, and the phenolic compounds (gallic, syringic, ferulic and ellagic acids, vanillin, syringaldehyde, coniferaldehyde, sinapaldehyde, umbelliferone and scopoletin) were analysed by a HPLC method developed and validated in our laboratory.4

The ANOVA results revealed that most of the compounds’ contents did not change significantly after 12 months of storage in bottle. In addition, the phenolic differences between the WSs resulting from the four ageing modalities remained, except for ferulic acid. Therefore, in these experimental conditions, this stage allowed preserving the phenolic composition imparted to the WS by the alternative ageing technology.

References

1 Canas S., 2017. Phenolic composition and related properties of aged wine spirits: Influence of barrel characteristics. A review. Beverages, 3, 55-76.
2 Canas S., Anjos O., Caldeira I., Belchior A.P., 2019. Phenolic profile and colour acquired by the wine spirit in the beginning of ageing: alternative technology using micro-oxygenation vs traditional technology. LWT – Food Science and Technology, 111, 260-269.
3 Granja-Soares J., Roque R., Cabrita M.J., Anjos O., Belchior A.P., Caldeira I., Canas S., 2020. Effect of innovative technology using staves and micro-oxygenation on the sensory and odorant profile of aged wine spirit. Food Chem., 333, 127450.
4 Canas S., Belchior A.P., Spranger M.I., Bruno de Sousa R., 2003. High-performance liquid chromatography method for analysis of phenolic acids, phenolic aldehydes and furanic derivatives in brandies. Development and validation. J. Sep. Sci., 26, 496–502.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Canas Sara1, Lourenço Sílvia1, Anjos Ofélia2 and Caldeira Ilda1

1Instituto Nacional de Investigação Agrária e Veterinária – Pólo de Dois Portos
2 Instituto Politécnico de Castelo Branco 

Contact the author

Keywords

wine spirit, storage in bottle, ageing technology, phenolic compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Grape berry size is a key factor in determining New Zealand Pinot noir wine composition

Making high quality but affordable Pinot noir (PN) wine is challenging in most terroirs and New Zealand’s (NZ) situation is no exception. To increase the probability of making highly typical PN wines producers choose to grow grapes in cool climates on lower fertility soils while adopting labour intensive practices. Stringent yield targets and higher input costs necessarily mean that PN wine cost is high, and profitability lower, in line-priced varietal wine ranges. To understand the reasons why higher yielding vines are perceived to produce wines of lower quality we have undertaken an extensive study of PN in NZ. Since 2018, we established a network of twelve trial sites in three NZ regions to find individual vines that produced acceptable commercial yields (above 2.5kg per vine) and wines of composition comparable to “Icon” labels. Approximately 20% of 660 grape lots (N = 135) were selected from within a narrow juice Total Soluble Solids (TSS) range and made into single vine wines under controlled conditions. Principal Component Analysis of the vine, berry, juice and wine parameters from three vintages found grape berry mass to be most effective clustering variable. As berry mass category decreased there was a systematic increase in the probability of higher berry red colour and total phenolics with a parallel increase in wine phenolics, changed aroma fraction and decreased juice amino acids. The influence of berry size on wine composition would appear stronger than the individual effects of vintage, region, vineyard or vine yield. Our observations support the hypothesis that it is possible to produce PN wines that fall within an “Icon” benchmark composition range at yields above 2.5kg per vine provided that the Leaf Area:Fruit Weight ratio is above 12cm2 per g, mean berry mass is below 1.2g and juice TSS is above 22°Brix.

Current climate change in the Oplenac wine-growing district (Serbia)

Serbian autochthonous vine varieties Smederevka (for white wines) and Prokupac (for rosé and red wines) are the primary representatives of typical characteristics of wines and terroir of numerous wine-growing areas in Serbia. In the past, these varieties were the leading vine varieties, however, as the result of globalization of winemaking and the trend of consumption of wines from widely prevalent vine varieties, they were replaced by introduced international varieties. Smederevka and Prokupac vine varieties are characterized by later time of grape ripening, and relative sensitivity to low temperatures. Climate conditions can be a restrictive factor for production of high-quality grapes and wine and for the spatial spreading of these varieties in hilly continental wine-growing areas.
This paper focuses on the spatial analysis of changes of main climate parameters, in particular, analysis of viticultural bioclimatic indices that were determined for the purposes of viticulture zoning of wine-growing areas in the period 1961-2010, and those same parameters determined for the current, that is, referential climate period (1988-2017). Results of the research, that is, analysis of climate changes indicate that the majority of examined climate parameters in the Oplenac wine-growing district improved from the perspective of Smederevka and Prokupac vine varieties. These studies of climate conditions indicate that changes of analyzed climate parameters, that is, bioclimatic indices will be favorable for cultivation of varieties with later grape ripening times and those more sensitive to low temperatures, such as the autochthonous vine varieties Smederevka and Prokupac, therefore, it is recommended to producers to more actively plant vineyards with these varieties in the territory of the Oplenac wine-growing district.

Mobile device to induce heat-stress on grapevine berries

Studying heat stress response of grapevine berries in the field often relies on weather conditions during the growing season. We constructed a mobile heating device, able to induce controlled heat stress on grapes in vineyards. The heater consisted of six 150 W infrared lamps mounted in a profile frame. Heating power of the lamps could be controlled individually by a control unit consisting of a single board computer and six temperature sensors to reach a pre-set temperature. The heat energy applied to individual berries within a cluster decreases by the squared distance to the heat source, enabling the establishment of temperature profiles within individual clusters. These profiles can be measured by infrared thermography once a steady state has been reached. Radiant flux density received by a berry depending on the distance was calculated based on a view factor and measured lamp surface temperature and resulted to 665 Wm-2 at 7cm. Infrared thermography of the fruit surface was in good agreement with measurements conducted with a thermocouple inserted at epidermis level. In combination with infrared thermography, the presented device offers possibilities for a wide range of applications like phenotyping for heat tolerance in the field to proceed in the understanding of the complex response of plants to heat stress. Sunburn necrosis symptoms were artificially induced with the aid of the device for cv. Bacchus and cv. Sylvaner in the 2020 and 2021 growing season. Threshold temperatures for sunburn induction (LT5030min) were derived from temperature data of single berries and visual sunburn assessment, applying logistic regression. A comparison of threshold temperatures for the occurrence of sunburn necrosis confirmed the higher susceptibility of cv. Bacchus. The lower susceptibility of cv. Sylvaner did not seem to be related to its phenolic composition, rendering a thermoprotective role of berry phenolic compounds unlikely.

The use of rootstock as a lever in the face of climate change and dieback of vineyard

As viticulture faces challenges such as climate change or vineyard dieback, the choice of the variety and rootstock becomes more and more crucial. To study rootstock levers in the Bordeaux region, a parcel of Cabernet Sauvignon (CS) was planted with four rootstocks in 2014. Twenty repetitions of each of the following four rootstocks were set up: 101-14 MGt, Nemadex AB, 420A MGt and Gravesac. The number of bunches, yields and pruning weights of the vine shoots were measured individually on 240 vines from 2017 to 2021. Since 2020, nitrogen status assessed by assimilable nitrogen level, hydric status assessed by δ13C and berry maturity were measured on 80 samples taken from 20 repetitions of the four rootstocks. A lower yield was measured for CS grafted onto Nemadex AB due to the lower number of bunches and the lower weight of berries. The differences between the other three rootstocks are small, but CS grafted onto 420A MGt was the most productive. The CS grafted onto Nemadex AB had the lowest pruning weight while 101-14 MGt had the highest. In 2020, δ13C showed a more moderate water stress with 101-14 MGt and 420A MGt than with Nemadex AB. Surprisingly, the Gravesac was under more stress than the 101-14 MGt. The nitrogen status in the berries was better for Nemadex AB but this was perhaps due to the significantly lower weight of the berries.Rootstock 101-14 MGt attained the highest accumulation of sugars in the berries while 420A MGt allows to preserve higher acidity. The parcel is still young which may explain some of the results. These measures must therefore be continued over the next several years to fully assess the effects of these rootstocks on the development of the vines and the quality of the production under new climatic conditions.

Spatial determination of areas in the Western Balkans region favorable for organic production

In problematic conditions for production of grapes and wine caused by the COVID-19 pandemic and the resulting occurrence of wine surpluses, producers are increasingly turning to the innovative viticulture and winemaking of products that are more appealing to the market and the consumers. On the other hand, consumption of the food safety or organic products, and therefore of organic grapes and wine, is increasingly common in the world, in particular in Europe. The Regional Rural Development Standing Working Group (SWG RRD), as a regional intergovernmental organization gathers actors in the viticulture and winemaking sector from states and territories of the Western Balkans (South-East Europe) in the Expert Working Group for Wine, with the aim of improving viticulture and winemaking in this region through joint activities. In accordance with the aforementioned, the SWG RRD is working on advancing organic production of grapes and wine, and on recognition of specificities of the terroir of wine-growing areas in Western Balkans. In addition, as part of the project “Facilitation of Exchange and Advice on Wine Regulations in Western Balkan Countries” helmed by the German Federal Ministry of Food and Agriculture, in addition to harmonization of relevant legislation with EU regulations, efforts are being invested towards recognition of organic wines. Within activities and project implemented by this organization, expert analyses and scientific research of the terroir of Western Balkans were carried out, and some of the results are presented in this paper.