IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Cell Walls Of Grape Mesocarp Possible Fining Agents For Red And White Wine

Cell Walls Of Grape Mesocarp Possible Fining Agents For Red And White Wine

Abstract

Clarification or fining of wines is a technique used in wineries to eliminate unwanted wine components, which negatively affect its quality. Clarification normally involves the addition of an adsorptive material that eliminates or reduces the presence of undesirable components. The problem is that many of the fining agents used in the industry contain allergens, such as caseinates or ovalbumin. The possibility of using plant cell wall material as finning agent has been previously studied [1,2]. Other possible fining agent could be the plant cell walls material from the pulp of the grape. This material is generated during the crushing of the grape and it presence could be a problem during the maceration stage, since it greatly reduces, by adsorption, the concentration of phenolic compounds in the wine. However, due to its great affinity for tannins, it could be used to reduce the wine astringency.The objective of this study was to analyze the ability of freeze-dried grape pulp plant cell walls to act as a clarifying agent in red wines of three different varieties: Cabernet Sauvignon, Syrah and Monastrell, and one white wine of the Airén variety. 0.5 g of the lyophilized cell walls were put in contact and mixed with 50 mL of the corresponding wine (tests in triplicate), and a contact time of 7 days was assayed, after which their chromatic characteristics and total tannin concentration were analyzed by spectrophotometry. Their anthocyanin and tannin composition were also analyzed by HPLC. The material was also tested for their ability to reduce the concentration of ochratoxin A and histamine in wines.The results showed that the freeze-dried cell walls of the grape pulp have a high capacity of retaining phenolic compounds, especially tannins, although there were differences between the varieties. The treated Cabernet Sauvignon wine showed the greatest reduction in tannin concentration after finning (23%), followed by Monastrell wine (18.3%) and Syrah wine (14.3%).A problem with most fining agents is that they not only bind to tannins, but also to anthocyanins. In this way, the freeze-dried walls of grape pulp also reduced the concentration of anthocyanins in the three red variety wines, although to a lesser extent than with tannins. In this case, there were practically no differences in anthocyanin reduction between the studied wines, Monastrell (12.3%), Syrah (11.3%) and Cabernet sauvignon (12.5%).In all the wines, the addition of this fining agent reduced the concentration of ochratoxin A by 50%. However, it was not so powerful when removing histamine, where only a reduction of 8% was achieved in Monastrell and Cabernet Sauvignon wines.In conclusion, grape pulp cell walls could be a fining agent that competes with other commercial agents currently used. In addition, this plant material is generated during the vinification, so their reuse as fining agent would contribute to a circular economy. 

References

1. Jiménez-Martínez, M. D., Gómez-Plaza, E., Molero, N., & Bautista-Ortín, A. B. (2017). Fining of red wines with pomace cell wall material: effect on wine phenolic composition. Food and Bioprocess Technology, 10(8), 1531-1539.
2. Jiménez-Martínez, M. D., Bautista-Ortín, A. B., Gil-Muñoz, R., & Gómez-Plaza, E. (2019). Fining with purified grape pomace. Effect of dose, contact time and varietal origin on the final wine phenolic composition. Food chemistry, 271, 570-576.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Osete-Alcaraz Andrea1, Ortega-Regules Ana E.2, Pérez-Porras Paula1, Bautista-Ortín Ana Belén1, Osete-Alcaraz Lucia1 and Gómez-Plaza Encarna1

1Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia
2Department of Chemical Engineering, Food and Environmental, University of Américas Puebla

Contact the author

Keywords

Anthocyanins, Tannins, fining agent, ochratoxin A, histamine.

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Elucidating vineyard site contributions to key sensory molecules: Identification of correlations between elemental composition and volatile aroma profile of site-specific Pinot noir wines

The reproducibility of elemental profile in wines produced across multiple vintages has been previously reported using grapes from a single scion clone of Vitis vinifera L. cv. Pinot noir. The grapevines were grown on fourteen different vineyard sites, from Oregon to southern California in the U.S.A., which span distances from approximately hundreds of meters to 1450 km, while elevations range from near sea level to nearly 500 m. In addition, sensorial (i.e. aroma, taste, and mouthfeel) and chemical (i.e. polyphenolic and volatile) differences across the different vineyard sites have also been observed among these wines at two aging time points. While strong evidence exists to support that grapes grown in different regions can produce wines with unique chemical and sensorial profiles, even when a single clone is used, the understanding of growing site characteristics that result in this reproducible differentiation continues to emerge. One hypothesis is that the elemental profile that a vineyard site imparts to the grape berries and the resulting wine is an important contributor to this differentiation in chemistry and sensory of wines. For example, various classes of enzymes that catalyze the formation of key aroma compounds or their precursors require specific metals. In this work, we begin to report correlations between elemental and volatile aroma profiles of site-specific Pinot noir wines, made under standardized winemaking conditions, that have been previously shown to be distinguished separately by these chemical analyses.

Use of multispectral satellite for monitoring vine water status in mediterranean areas

The development of new generations of multispectral satellites such as Sentinel-2 opens possibilities as to vine water status assessment (Cohen et al., 2019). Based on a three years field campaign, a model of Stem Water Potential (SWP) estimation on vine using four satellite bands in Red, Red-Edge, NIR and SWIR domains was developed (Laroche-Pinel et al., 2021). The model relies on SWP field measures done using a pressure chamber (Scholander et al., 1965), which is a common, robust and precise method to assess vine water status (Acevedo-Opazo et al., 2008). The model was mainly developed from from SWP measures on Syrah N (Laroche Pinel E., 2021).

A large scale monitoring was organized in different vineyards in the Mediterranean region in 2021. 10 varieties amongst the most represented in this area were monitored (Cabernet sauvignon N, Chardonnay B, Cinsault N, Grenache N, Merlot N, Mourvèdre N, Sauvignon B, Syrah N, Vermentino B, Viognier B). The model was used to produce water status maps from Sentinel-2 images, starting from the beginning of June (fruit set) up to September (harvest). The average estimated SWP for each vine was compared to actual field SWP measures done by wine growers or technicians during usual monitoring of irrigation programs. The correlations between mean estimated SWP and mean measured SWP were at the same level than expected by the model. (Laroche Pinel, 2021) The general SWP kinetics were comparable. The estimated SWP would have led to same irrigation decisions concerning the date of first irrigation in comparison with measured SWP.

Acevedo-Opazo, C., Tisseyre, B., Ojeda, H., Ortega-Farias, S., Guillaume, S. (2008). Is it possible to assess the spatial variability of vine water status? OENO One, 42(4), 203.
Cohen, Y., Gogumalla, P., Bahat, I., Netzer, Y., Ben-Gal, A., Lenski, I., … Helman, D. (2019). Can time series of multispectral satellite images be used to estimate stem water potential in vineyards? In Precision agriculture ’19, The Netherlands: Wageningen Academic Publishers, pp. 445–451.
Laroche-Pinel, E., Duthoit, S., Albughdadi, M., Costard, A. D., Rousseau, J., Chéret, V., & Clenet, H. (2021). Towards vine water status monitoring on a large scale using sentinel-2 images. remote sensing, 13(9), 1837.
Laroche-Pinel,E. (2021). Suivi du statut hydrique de la vigne par télédétection hyper et multispectrale. Thèse INP Toulouse, France.
Scholander, P.F., Bradstreet, E.D., Hemmingsen, E.A., & Hammel, H.T. (1965). Sap pressure in vascular plants: Negative hydrostatic pressure can be measured in plants. Science, 148(3668), 339–346.

Drought effect on aromatic and phenolic potential of seven recovered grapevine varieties in Castilla-La Mancha region (Spain)

The effects of climate change are seriously affecting the quality of wine grapes. High temperatures and drought cause imbalances in the chemical composition of grapes. The result is overripe grapes with low acidity and high sugar content, which produce wines with excessive alcohol content, lacking in freshness and not very aromatic. As a consequence, the search of varieties with capacity of produce quality grapes in adverse climate conditions is a good alternative to preserve the sustainability of vineyards. In this work, quality parameters of seven Vitis vinifera L. cultivars (five whites and two reds) recently recovered from extinction and grown under two different hydric regimes (rainfed and irrigated) were analyzed during the 2020 vintage. At harvest time, weight of 100 berries, must physicochemical parameters (brix degree, total acidity, malic acid, pH), and carbon and oxygen isotope ratios (δ13C, δ18O) were determined. Subsequently, varietal aroma potential index (IPAv) and total polyphenol index (TPI) were analyzed. Quality parameters, IPAv and TPI, showed significant differences between varieties and water regimes. Both red varieties, Moribel and Tinto Fragoso, stood out for their high aromatic and phenolic potential, which was higher under rainfed regime. Regarding to white varieties, Montonera del Casar and Jarrosuelto stood out in terms of varietal aroma potential. Montonera del Casar high acidity in its musts and Jarrosuelto showed the highest berry weights.

Organic recycled mulches in sustainable viticulture: assessment of spontaneous plants communities and weed coverage

In recent years, developing more efficient and sustainable viticulture management has been essential due to the impact of climate change in semiarid regions. For this reason, the use of recycled organic mulching (ROM) in the vineyard has become an interesting strategy to cope with water stress, isolated soil from extreme temperatures and improving soil humidity, control the presence of weeds and therefore reduce the inputs of herbicides and improve soil fertility. This work aimed to analyse the effect of three different organic mulches [straw (S), grape pruning debris (GPD) and spent mushroom compost (SMC)] and two traditional soil management techniques [herbicide (H) and interrow (IN)] on weed coverage and the spontaneous plant communities’ presence. Data sampling was collected throughout the vine vegetative cycle of 2021 in La Rioja, Spain. The different soil management techniques had a clear effect on weed coverage and his development during the vine vegetative cycle. SMC and H were the treatments with the highest and the lowest coverage percentage, respectively. IN had a delayed weed emergence at the beginning of the vine vegetative cycle, but finally it reached maximum values nearby SMC. GPD and S had similar effects on weed emergence, reaching 25-30% of the maximum coverage values. A total of 29 herbaceous species were identified during the vegetative cycle, some of them very isolated and occasional. Principal component analysis (PCAs) showed a good association between spontaneous species and treatments, furthermore, specific species-treatment associations were found. Moreover, three clear groups of herbaceous communities were identified by cluster analysis. This study provides interesting information about the effect of different alternative soil management on herbaceous plant coverage and weed species communities which could contribute to making more sustainable viticulture.

The impact of sustainable management regimes on amino acid profiles in grape juice, grape skin flavonoids, and hydroxycinnamic acids

One of the biggest challenges of agriculture today is maintaining food safety and food quality while providing ecosystem services such as biodiversity conservation, pest and disease control, ensuring water quality and supply, and climate regulation. Organic farming was shown to promote biodiversity and carbon sequestration, and is therefore seen as one possibility of environmentally friendly production. Consumers expect organically grown crops to be free from chemical pesticides and mineral fertilizers and often presume that the quality of organically grown crops is different or higher compared to conventionally grown crops. Integrated, organic, and biodynamic viticulture were compared in a replicated field trial in Geisenheim, Germany (Vitis vinifera L. cv. Riesling). Amino acid profiles in juice, grape skin flavonoids, and hydroxycinnamic acids were monitored over three consecutive seasons beginning 7 years after conversion to organic and biodynamic viticulture, respectively. In addition, parameters such as soil nutrient status, yield, vigor, canopy temperature, and water stress were monitored to draw conclusions on reasons for the observed changes. Results revealed that the different sustainable management regimes highly differed in their amino acid profiles in juice and also in their skin flavonol content, whereas differences in the flavanol and hydroxycinnamic acid content were less pronounced. It is very likely that differences in nutrient status and yield determined amino acid profiles in juice, although all three systems showed similar amounts of mineralized nitrogen in the soil. Canopy structure and temperature in the bunch zone did not differ among treatments and therefore cannot account for the observed differences in favonols. A different light exposure of the bunches in the respective systems due to differences in vigor together with differences in berry size and a different water status of the vines might rather be responsible for the increase in flavonol content under organic and biodynamic viticulture.