IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The effect of sulfur compounds on the formation of varietal thiols in Sauvignon Blanc and Istrian Malvasia wines

The effect of sulfur compounds on the formation of varietal thiols in Sauvignon Blanc and Istrian Malvasia wines

Abstract

Varietal thiols 3-sulfanylhexan-1-ol (3SH), 3-sulfanylhexyl acetate (3SHA) and 4-methyl-4-sulfanylpentan-2-one (4SMP) are essential for fruity aromas of Sauvignon Blanc wines. The concentration of varietal thiols in wines was thought to be related to the concentration of their precursors in grapes, however only a small proportion of precursors are released to varietal thiols during fermentation. New findings suggested that specific grape juice metabolites could significantly impact on the development of three major varietal thiols and other aroma compounds of Sauvignon Blanc wines. Among them, elemental sulfur and hydrogen sulfide indicated a positive role on the formation of varietal thiols during vinification (Araujo et al., 2016; Harsch et al., 2013). This research aimed to investigate the addition of different sources of sulfur in grape juice on formation of 3SH, 3SHA, 4SMP and benzene methanethiol (BM) in Sauvignon Blanc and Istrian Malvasia wines. Istrian Malvasia (Vitis vinifera L.) is a local grapevine variety which sometimes aromatically resemble on Sauvignon Blanc.Pressed grape juice was settled overnight and sampled from tank for microfermentations. Additions of different sulfur compounds in 700 mL of juice were performed just before yeast inoculation in four replicates each: (1) control – no addition, (2) addition of reduced glutathione (GSH) (20 and 50 mg/L), (3) addition of methionine (10 and 20 mg/L), (4) addition of cysteine (20 and 40 mg/L), (5) addition of elemental sulfur (5 and 10 mg/L) and (6) addition of  sodium hydrosulfide hydrate (1 and 10 mg/L). Fermentations were performed with Saccharomyces cerevisae (X5, Laffort, France) in 750 mL dark glass bottles at 18-20 °C. After fermentation, 50 mg/L SO2 was added, wines were settled, racked, and stored at -20 °C until analyses. Varietal thiols were analysed by GC-MS/MS (Schimadzu, TQ8050) using the modified method as described (Šuklje et al., 2013).Sauvignon Blanc wines with the addition of 10 mg/L NaSH·xH2O (which releases hydrogen sulfide) had significant higher concentration of 3SH and 3SHA compared to control and other treatments. Istrian Malvasia wines with the addition of 10 mg/L NaSH·xH2O had significant higher concentrations of 3SH but there were no significant differences in 3SHA.  The addition of elemental sulfur increased 3SH and 3SHA in both varieties, however not significantly. Addition of sulphur compounds in our study had no effect on concentration of 4MMP and BM in Sauvignon Blanc or Istrian Malvasia wines. On the other hand, the additions of GSH and cysteine resulted in significantly lower formation of 3SH and 3SHA in Sauvignon Blanc wines in comparison to control and other additions. The latter finding is compliant with the study on model wines by Alegre et al. (2019).Modifying sulfur source composition in grape juice showed some significant changes in concentration of 3SH in both Sauvignon Blanc and Istrian Malvasia wines.

References

Araujo D. L., Vannevel S., Buica A., Callerot S., Fedrizzi B., Kilmartin A. P., du Toit J. W. 2016. Indications of the prominent role of elemental sulfur in the formation of the varietal thiol 3-mercaptohexanol in Sauvignon blanc wine, Food Research International, 98, 79-86.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Vanzo Andreja1, Alenka Mihelčič1, Katja Šuklje1, Klemen Lisjak1

1 Agricultural Institute of Slovenia

Contact the author

Keywords

sulfur compounds, grape juice, varietal thiols, Sauvignon Blanc, Istrian Malvasia

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

The modification of cultural practices in grapevine cv. Syrah, does it modify the characteristics of the musts?

The work shows the results of a year of experimentation (2020) in a Syrah variety vineyard in La Roda (Castilla-La Mancha, Spain). The trial approach was on a randomized block design with two factors: Irrigation (I) and Pruning (P). Irrigation schedules were adjusted to apply amounts close to 1,500 m3/ha. With this provision, 2 different irrigation treatments were proposed: I1) Start of irrigation from pea-sized grape to post-harvest (providing at least 20 % of the total amount of irrigation water to be provided post-harvest); I2) Start of irrigation from pea-sized grape to harvest (usual irrigation practice in the study area). Pruning was proposed with two treatments, one at the end of January (P1), which is pruning on a conventional date; and P2) pruning carried out at the beginning of budding. In total, 4 repetitions were designed with 4 elementary plots, each one of them representing one of the proposed treatments (I1P1; I1P2; I2P1; I2P2). In total, 16 plots were worked on and each elementary plot consisted of 30 strains, distributed in 3 lines. The productive response was evaluated with the yield results of the harvest harvested at 23 ºBrix. The qualitative response was measured in the musts through the indices of technological (acidity, pH and potassium) and phenolic maturity and aromatic compounds in free and glycosylated fractions. The treatments tested had, in general, an effect on the different variables analyzed.

Permanent cover cropping with reduced tillage increased resiliency of wine grape vineyards to climate change

Majority of California’s vineyards rely on supplemental irrigation to overcome abiotic stressors. In the context of climate change, increases in growing season temperatures and crop evapotranspiration pose a risk to adaptation of viticulture to climate change. Vineyard cover crops may mitigate soil erosion and preserve water resources; but there is a lack of information on how they contribute to vineyard resiliency under tillage systems. The aim of this study was to identify the optimum combination of cover crop sand tillage without adversely affecting productivity while preserving plant water status. Two experiments in two contrasting climatic regions were conducted with two cover crops, including a permanent short stature grass (P. bulbosa hybrid), barley (Hordeum spp), and resident vegetation under till vs. no-till systems in a Ruby Cabernet (V. vinifera spp.) (Fresno) and a Cabernet Sauvingon (Napa) vineyard. Results indicated that permanent grass under no-till preserved plant available water until E-L stage 17. Consequently, net carbon assimilation of the permanent grass under no-till system was enhanced compared to those with barley and resident vegetation. On the other hand, the barley under no-till system reduced grapevine net carbon assimilation during berry ripening that led to lower content of nonstructural carbohydrates in shoots at dormancy. Components of yield and berry composition including flavonoid profile at either site were not adversely affected by factors studied. Switching to a permanent cover crop under a no-till system also provided a 9% and 3% benefit in cultural practices costs in Fresno and Napa, respectively. The results of this work provides fundamental information to growers in preserving resiliency of vineyard systems in hot and warm climate regions under context of climate change.

δ13C : A still underused indicator in precision viticulture  

The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
The lack of private laboratories proposing the analysis, the cost of the technology, as well as the long analytical delays, have been detrimental to its development. Some laboratories tried to overcome the analytical difficulties of isotopic analysis by using fourier transformed infrared spectroscopy, as a fast and cheap alternative to the official OIV method (IRMS). These claimed FTIR models have never been published or peer reviewed and cannot be considered robust. In this work, thanks to the recent acquisition of IRMS technology, new modern and robust applications of δ13C for viticulture are proposed. This includes the use of the analysis to make parcel separations at harvesting, the possibility to increase the precision of hydric stress cartography and the potential cost reduction when compared with Scholander pressure bomb analysis.

The combined effects of climate, soils, and deficit irrigation on yield and quality of Touriga Nacional under high atmospheric demand in the Douro Region

Global warming is one of the biggest environmental, social and economic threats in several viticultural regions. In the Douro Valley, changes are expected in the coming years, namely an increase in temperature and a decrease in precipitation. These changes are likely to have consequences for the production and quality of wine.
The aim of this study was to explore the effects of different soil characteristics combined with several deficit irrigation strategies, managed throughout ETc references and predawn leaf water potentials thresholds, on physiology, yield, and qualitative attributes on the Touriga Nacional variety under years of mild to severe water and heat stress.
The studies were conducted over seven years (2015 to 2021) in two plots of a commercial vineyard located at Quinta do Ataíde (Symington Family Estates) planted in 2011 and 2014 at 170 meters elevation, growing under three water regimes: non-irrigated (NI) and two deficit irrigation strategies (30% and 60% ETc) assessed weekly by Ψpd. The site has an annual rainfall below 500 mm, with high atmospheric demand. Climate data was collected from a weather station, located on site. Berry ripening was followed weekly for fruit analysis. At harvest, yield, vigour and pruning weight per vine were determined from 90 vines by treatment. Each season at veraison the NDVI Index was accessed by a drone. The soils physic-chemistry in the experimental blocs were analysed and grouped by SWHC. Delta C-13 analyses were also performed per treatment in two years.Irrigation had a positive effect on yield per vine, mostly due to an increase in berry and cluster weight, and fertility index through the years. A significant increase in sugar content, colour and phenols was observed with deficit irrigation in some years, but vine vigour related to soil characteristics had by far the greatest impact on quality.

Rapid damage assessment and grapevine recovery after fire

There is increasing scientific consensus that climate changeis the underlying cause of the prolonged dry and hot conditions that have increased the risk of extreme fire weather in many countries around the world. In December 2019, a bushfire event occurred in the Adelaide Hills, South Australia where 25,000 hectares were burnt and in vineyards and surrounding areas various degrees of scorching and infrastructure damage occurred. The ability to coordinate and plan recovery after a fire event relies on robust and timely data. The current practice for measuring the scale and distribution of fire damage is to walk or drive the vineyard and score individual vines based on visual observation. The process is time consuming, subjective, or semi-quantitative at best. After the December 2019 fires, it took many months to access properties and estimate the area of vineyard damaged. This study compares the rapid assessment and mapping of fire damage using high-resolution satellite imagery with more traditional ground based measures. Satellite imagery tracking vineyard recovery in the season following the bushfire is being correlated to field assessments of vineyard productivity such as canopy health and development, fertility and carbohydrate storage. Canopy health in the seasons following the fires correlated to the severity of the initial fire damage. Severely damaged vines had reduced canopy growth, were infertile or had very low fertility as well as lower carbohydrate levels in buds and canes during dormancy, which reduced productivity in the seasons following the bushfire event. In contrast, vines that received minor damage were able to recover within 1-2 years. Tools that rapidly and affordably capture the extent and severity of damage over large vineyard area will allow producers, government and industry bodies to manage decisions in relation to fire recovery planning, coordination and delivery, improving the efficiency and effectiveness of their response.