IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The effect of sulfur compounds on the formation of varietal thiols in Sauvignon Blanc and Istrian Malvasia wines

The effect of sulfur compounds on the formation of varietal thiols in Sauvignon Blanc and Istrian Malvasia wines

Abstract

Varietal thiols 3-sulfanylhexan-1-ol (3SH), 3-sulfanylhexyl acetate (3SHA) and 4-methyl-4-sulfanylpentan-2-one (4SMP) are essential for fruity aromas of Sauvignon Blanc wines. The concentration of varietal thiols in wines was thought to be related to the concentration of their precursors in grapes, however only a small proportion of precursors are released to varietal thiols during fermentation. New findings suggested that specific grape juice metabolites could significantly impact on the development of three major varietal thiols and other aroma compounds of Sauvignon Blanc wines. Among them, elemental sulfur and hydrogen sulfide indicated a positive role on the formation of varietal thiols during vinification (Araujo et al., 2016; Harsch et al., 2013). This research aimed to investigate the addition of different sources of sulfur in grape juice on formation of 3SH, 3SHA, 4SMP and benzene methanethiol (BM) in Sauvignon Blanc and Istrian Malvasia wines. Istrian Malvasia (Vitis vinifera L.) is a local grapevine variety which sometimes aromatically resemble on Sauvignon Blanc.Pressed grape juice was settled overnight and sampled from tank for microfermentations. Additions of different sulfur compounds in 700 mL of juice were performed just before yeast inoculation in four replicates each: (1) control – no addition, (2) addition of reduced glutathione (GSH) (20 and 50 mg/L), (3) addition of methionine (10 and 20 mg/L), (4) addition of cysteine (20 and 40 mg/L), (5) addition of elemental sulfur (5 and 10 mg/L) and (6) addition of  sodium hydrosulfide hydrate (1 and 10 mg/L). Fermentations were performed with Saccharomyces cerevisae (X5, Laffort, France) in 750 mL dark glass bottles at 18-20 °C. After fermentation, 50 mg/L SO2 was added, wines were settled, racked, and stored at -20 °C until analyses. Varietal thiols were analysed by GC-MS/MS (Schimadzu, TQ8050) using the modified method as described (Šuklje et al., 2013).Sauvignon Blanc wines with the addition of 10 mg/L NaSH·xH2O (which releases hydrogen sulfide) had significant higher concentration of 3SH and 3SHA compared to control and other treatments. Istrian Malvasia wines with the addition of 10 mg/L NaSH·xH2O had significant higher concentrations of 3SH but there were no significant differences in 3SHA.  The addition of elemental sulfur increased 3SH and 3SHA in both varieties, however not significantly. Addition of sulphur compounds in our study had no effect on concentration of 4MMP and BM in Sauvignon Blanc or Istrian Malvasia wines. On the other hand, the additions of GSH and cysteine resulted in significantly lower formation of 3SH and 3SHA in Sauvignon Blanc wines in comparison to control and other additions. The latter finding is compliant with the study on model wines by Alegre et al. (2019).Modifying sulfur source composition in grape juice showed some significant changes in concentration of 3SH in both Sauvignon Blanc and Istrian Malvasia wines.

References

Araujo D. L., Vannevel S., Buica A., Callerot S., Fedrizzi B., Kilmartin A. P., du Toit J. W. 2016. Indications of the prominent role of elemental sulfur in the formation of the varietal thiol 3-mercaptohexanol in Sauvignon blanc wine, Food Research International, 98, 79-86.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Vanzo Andreja1, Alenka Mihelčič1, Katja Šuklje1, Klemen Lisjak1

1 Agricultural Institute of Slovenia

Contact the author

Keywords

sulfur compounds, grape juice, varietal thiols, Sauvignon Blanc, Istrian Malvasia

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include: • New user interface • User authentication • Batch analysis of multiple images • Ease the learning curve through enhanced help features • Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard. Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

Teasing apart terroir: the influence of management style on native yeast communities within Oregon wineries and vineyards

Newer sequencing technologies have allowed for the addition of microbes to the story of terroir. The same environmental factors that influence the phenotypic expression of a crop also shape the composition of the microbial communities found on that crop. For fermented goods, such as wine, that microbial community ultimately influences the organoleptic properties of the final product that is delivered to customers. Recent studies have begun to study the biogeography of wine-associated microbes within different growing regions, finding that communities are distinct across landscapes. Despite this new knowledge, there are still many questions about what factors drive these differences. Our goal was to quantify differences in yeast communities due to management style between seven pairs of conventional and biodynamic vineyards (14 in total) throughout Oregon, USA. We wanted to answer the following questions: 1) are yeast communities distinct between biodynamic vineyards and conventional vineyards? 2) are these differences consistent across a large geographic region? 3) can differences in yeast communities be tied to differences in metabolite profiles of the bottled wine? To collect our data we took soil, bark, leaf, and grape samples from within each vineyard from five different vines of pinot noir. We also collected must and a 10º brix sample from each winery. Using these samples, we performed 18S amplicon sequencing to identify the yeast present. We then used metabolomics to characterize the organoleptic compounds present in the bottled wine from the blocks the year that we sampled. We are actively in the process of analysing our data from this study.

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.

Modeling the suitability of Pinot Noir in Oregon’s Willamette Valley in a changing climate

Air temperature is the key driver of grapevine phenology and a significant environmental factor impacting yield and quality for a winegrape growing region. In this study the optimal downscaled CMIP5 ensemble for computing thegrowing season average temperature (GST) viticulture climate classification index was determined to spatially compute on a decadal basis predictions of the GST climate index and the grapevine sugar ripeness (GSR) model for Pinot Noir throughout the Willamette Valley (WV) American Viticultural Area (AVA). Forecasts for average temperature and a 220 g/L target sugar concentration level were computed using daily Localized Constructed Analogs (LOCA) downscaled CMIP5 historic and Representative Concentration Pathways (RCP) future climate projections of minimum and maximum daily temperature. We explore spatiotemporal trends of the GST climate classification index and Pinot Noir specific applications of the GSR phenology model for the WV AVA. Spatiotemporal computations of the GST climate index and Pinot Noir specific applications of the GSR model enable the opportunity to explore relationships between their computed values with one intent being to provide updated GST ranges that better align with current temperature-based modeling understanding of Pinot Noir grapevine phenology and the viticultural application of LOCA CMIP5 climate projections for the WV AVA. The Pinot Noir specific applications of the GSR model or the GST index with updated bounds indicate that the percent of the WV AVA area suitable for Pinot Noir production is currently at or near its peak value in the upper 80s to lower 90s of this century.

Grapevine sugar concentration model in the Douro Superior, Portugal

Increasingly warm and dry climate conditions are challenging the viticulture and winemaking sector. Digital technologies and crop modelling bear the promise to provide practical answers to those challenges. As viticultural activities strongly depend on harvest date, its early prediction is particularly important, since the success of winemaking practices largely depends upon this key event, which should be based on an accurate and advanced plan of the annual cycle. Herein, we demonstrate the creation of modelling tools to assess grape ripeness, through sugar concentration monitoring. The study area, the Portuguese Côa valley wine region, represents an important terroir in the “Douro Superior” subregion. Two varieties (cv. Touriga Nacional and Touriga Franca) grown in five locations across the Côa Region were considered. Sugar accumulation in grapes, with concentrations between 170 and 230 g l-1, was used from 2014 to 2020 as an indicator of technological maturity conditioned by meteorological factors. The climatic time series were retrieved from the EU Copernicus Service, while sugar data were collected by a non-profit organization, ADVID, and by Sogrape, a leading wine company. The software for calibrating and validating this model framework was the Phenology Modeling Platform (PMP), version 5.5, using Sigmoid and growing degree-day (GDD) models for predictions. The performance was assessed through two metrics: Roots Mean Square Error (RMSE) and efficiency coefficient (EFF), while validation was undertaken using leave-one-out cross-validation. Our findings demonstrate that sugar content is mainly dependent on temperature and air humidity. The models achieved a performance of 0.65