IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The effect of sulfur compounds on the formation of varietal thiols in Sauvignon Blanc and Istrian Malvasia wines

The effect of sulfur compounds on the formation of varietal thiols in Sauvignon Blanc and Istrian Malvasia wines

Abstract

Varietal thiols 3-sulfanylhexan-1-ol (3SH), 3-sulfanylhexyl acetate (3SHA) and 4-methyl-4-sulfanylpentan-2-one (4SMP) are essential for fruity aromas of Sauvignon Blanc wines. The concentration of varietal thiols in wines was thought to be related to the concentration of their precursors in grapes, however only a small proportion of precursors are released to varietal thiols during fermentation. New findings suggested that specific grape juice metabolites could significantly impact on the development of three major varietal thiols and other aroma compounds of Sauvignon Blanc wines. Among them, elemental sulfur and hydrogen sulfide indicated a positive role on the formation of varietal thiols during vinification (Araujo et al., 2016; Harsch et al., 2013). This research aimed to investigate the addition of different sources of sulfur in grape juice on formation of 3SH, 3SHA, 4SMP and benzene methanethiol (BM) in Sauvignon Blanc and Istrian Malvasia wines. Istrian Malvasia (Vitis vinifera L.) is a local grapevine variety which sometimes aromatically resemble on Sauvignon Blanc.Pressed grape juice was settled overnight and sampled from tank for microfermentations. Additions of different sulfur compounds in 700 mL of juice were performed just before yeast inoculation in four replicates each: (1) control – no addition, (2) addition of reduced glutathione (GSH) (20 and 50 mg/L), (3) addition of methionine (10 and 20 mg/L), (4) addition of cysteine (20 and 40 mg/L), (5) addition of elemental sulfur (5 and 10 mg/L) and (6) addition of  sodium hydrosulfide hydrate (1 and 10 mg/L). Fermentations were performed with Saccharomyces cerevisae (X5, Laffort, France) in 750 mL dark glass bottles at 18-20 °C. After fermentation, 50 mg/L SO2 was added, wines were settled, racked, and stored at -20 °C until analyses. Varietal thiols were analysed by GC-MS/MS (Schimadzu, TQ8050) using the modified method as described (Šuklje et al., 2013).Sauvignon Blanc wines with the addition of 10 mg/L NaSH·xH2O (which releases hydrogen sulfide) had significant higher concentration of 3SH and 3SHA compared to control and other treatments. Istrian Malvasia wines with the addition of 10 mg/L NaSH·xH2O had significant higher concentrations of 3SH but there were no significant differences in 3SHA.  The addition of elemental sulfur increased 3SH and 3SHA in both varieties, however not significantly. Addition of sulphur compounds in our study had no effect on concentration of 4MMP and BM in Sauvignon Blanc or Istrian Malvasia wines. On the other hand, the additions of GSH and cysteine resulted in significantly lower formation of 3SH and 3SHA in Sauvignon Blanc wines in comparison to control and other additions. The latter finding is compliant with the study on model wines by Alegre et al. (2019).Modifying sulfur source composition in grape juice showed some significant changes in concentration of 3SH in both Sauvignon Blanc and Istrian Malvasia wines.

References

Araujo D. L., Vannevel S., Buica A., Callerot S., Fedrizzi B., Kilmartin A. P., du Toit J. W. 2016. Indications of the prominent role of elemental sulfur in the formation of the varietal thiol 3-mercaptohexanol in Sauvignon blanc wine, Food Research International, 98, 79-86.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Vanzo Andreja1, Alenka Mihelčič1, Katja Šuklje1, Klemen Lisjak1

1 Agricultural Institute of Slovenia

Contact the author

Keywords

sulfur compounds, grape juice, varietal thiols, Sauvignon Blanc, Istrian Malvasia

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Grapevine xylem embolism resistance spectrum reveals which varieties have a lower mortality risk in a future dry climate

Wine growing regions have recently faced intense and frequent droughts that have led to substantial economical losses, and the maintenance of grapevine productivity under warmer and drier climate will rely notably on planting drought-resistant cultivars. Given that plant growth and yield depend on water transport efficiency and maintenance of photosynthesis, thus on the preservation of the vascular system integrity during drought, a better understanding of drought-related hydraulic traits that have a significant impact on physiological processes is urgently needed. We have worked towards this end by assessing vulnerability to xylem embolism in 30 grapevine commercial varieties encompassing red and white Vitis vinifera varieties, hybrid varieties characterized by a polygenic resistance for powdery and downy mildew, and commonly used rootstocks. These analyses further allowed a global assessment of wine regions with respect to their varietal diversity and resulting vulnerability to stem embolism. Hybrid cultivars displayed the highest vulnerability to embolism, while rootstocks showed the greatest resistance. Significant variability also arose among Vitis vinifera varieties, with Ψ12 and Ψ50 values ranging from -0.4 to -2.7 MPa and from -1.8 to -3.4 MPa, respectively. Cabernet franc, Chardonnay and Ugni blanc featured among the most vulnerable varieties while Pinot noir, Merlot and Cabernet Sauvignon ranked among the most resistant. In consequence, wine regions bearing a significant proportion of vulnerable varieties, such as Poitou-Charentes, France and Marlborough, New Zealand, turned out to be at greater risk under drought. These results highlight that grapevine varieties may not respond equally to warmer and drier conditions, outlining the importance to consider hydraulic traits associated with plant drought tolerance into breeding programmes and modeling simulations of grapevine yield maintenance under severe drought. They finally represent a step forward to advise the wine industry about which varieties and regions would have the lowest risk of drought-induced mortality under climate change.

Better understand the soil wet bulb formation with subsurface or aerial drip irrigation in viticulture

The gradual change in rainfall patterns experienced in the south of France vineyards, especially around the Mediterranean sea, means that the vines are increasingly subject to summer drought. The winegrowers developped the use of irrigation techniques to ensure the maintenance of competitive yields in the production of wines under Protected Geographical Indication label. In practice, drip irrigation pipes can be installed above the ground or buried into the soil as well as at different distances from the vine row. The objective of this study was to examine the profiles of the wet bulbs of the soil obtained from two drip irrigation systems : aerial drip located under the vine row and subsurface drip placed in the middle of the inter-row. This experiment took place over two consecutive seasons (2020-2021) on a 3.4 ha Viognier plot in the Mediterranean region (PGI Oc, France) on sandy clay soil. The annual rainfalls were less than 400 mm. Soil water content probes were installed at different depths (20 – 40 – 60 – 80 cm) and at different lateralities from the vine row (30 – 60 – 90 – 120 cm) to control the formation of the soil wet bulb during irrigation. The mapping and the analysis of the data allowed a better understanding and differentiation of the water percolation when irrigating with subsurface or aerial drip. For the same amount of water and without differences of vine water status, it is shown that in a subsurface drip irrigation situation, the size of the wet bulb formed is larger than in aerial drip irrigation system.

Influence of climatic conditions on grape composition of Tempranillo in La Mancha DO (Spain)

The aim of this work was to analyze the variability in grape composition of the Tempranillo cultivar related to climatic conditions, in La Mancha Designation of Origin. Grape composition (sugar content, total acidity, pH, malic acid, and total and extractable anthocyanins) recorded during ripening, were analysed for the period 2000-2019. The weather conditions at daily time scale, recorded during the same period, were also evaluated. The relationships between grape parameters with climatic variables related to temperature and to water deficits, referring different periods between phenological events along the growing cycle, were evaluated using regression analysis. High variability in grape composition was observed in the period analysed. Total acidity varied between 3.7 and 7.3 gL-1 while malic acid varied between 1.2 and 4 gL-1. The extractable anthocyanins ranged between 526 and 972 mgL-1, and total anthocyanins ranged between 922 and 1388 mgL-1, being the lowest values recorded in the hottest year (2017). Total acidity decreased 0.77 gL-1 for an increase of 100 GDD, while malic acid decrease in 0.42 gL-1 for the same GDD increase, being the period between veraison and harvest the one that seemed to have higher influence on acidity. In addition, it was confirmed that increasing water deficits decreased acidity. Total and extractable anthocyanins increased in about 210 and 105 mgL-1, respectively, with an increase of 100 GDD from veraison to harvest, and the increase in water deficits favour the increase of anthocyanins, both total and extractable anthocyanins. Total and extractable anthocyanins concentration increased in 35 and 22 mgL-1 per an increase of 10 mm in the water deficit. These results can be of interest to understand the potential changes that grapes composition may suffer under future warmer climates.

Modelling vine water stress during a critical period and potential yield reduction rate in European wine regions: a retrospective analysis

Most European vineyards are managed under rainfed conditions, where seasonal water deficit has become increasingly important. The flowering-veraison phenophase represents an important period for vine response to water stress, which is seldomly thoroughly evaluated. Therefore, we aim to quantify the flowering-veraison water stress levels using Crop Water Stress Indicator (CWSI) over 1986–2015 for important European wine regions, and to assess the respective potential Yield Lose Rate (YLR). Additionally, we also investigate whether an advanced flowering-veraison phase may help alleviating the water stress with improved yield. A process-based grapevine model STICS is employed, which has been extensively calibrated for flowering and veraison stages using observed data at 38 locations with 10 different grapevine varieties. Subsequently, the model is being implemented at the regional level, considering site-specific calibration results and gridded climate and soil datasets. The findings suggest wine regions with stronger flowering-veraison CWSI tend to have higher potential YLR. However, contrasting patterns are found between wine regions in France-Germany-Luxembourg and Italy-Portugal-Spain. The former tends to have slight-to-moderate drought conditions (CWSI<0.5) and a negligible-to-moderate YLR (<30%), whereas the latter possesses severe-to-extreme CWSI (>0.5) and substantial YLR (>40%). Wine regions prone to a high drought risk (CWSI>0.75) are also identified, which are concentrated in southern Mediterranean Europe. An advanced flowering-veraison phase may have benefited from cooler temperatures and a higher fraction of spring precipitation in wine regions of Italy-Portugal-Spain, resulting in alleviated CWSI and moderate reductions of YLR. For those of France-Germany-Luxembourg, this can have reduced flowering-veraison precipitation, but prevalent alleviations of YLR are also found, possibly because of shifted phase towards a cooler growing season with reduced evaporative demands. Overall, such a retrospective analysis might provide new insights towards better management of seasonal water deficit for conventionally vulnerable Mediterranean wine regions, but also for relatively cooler and wetter Central European regions.

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares