IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The effect of sulfur compounds on the formation of varietal thiols in Sauvignon Blanc and Istrian Malvasia wines

The effect of sulfur compounds on the formation of varietal thiols in Sauvignon Blanc and Istrian Malvasia wines

Abstract

Varietal thiols 3-sulfanylhexan-1-ol (3SH), 3-sulfanylhexyl acetate (3SHA) and 4-methyl-4-sulfanylpentan-2-one (4SMP) are essential for fruity aromas of Sauvignon Blanc wines. The concentration of varietal thiols in wines was thought to be related to the concentration of their precursors in grapes, however only a small proportion of precursors are released to varietal thiols during fermentation. New findings suggested that specific grape juice metabolites could significantly impact on the development of three major varietal thiols and other aroma compounds of Sauvignon Blanc wines. Among them, elemental sulfur and hydrogen sulfide indicated a positive role on the formation of varietal thiols during vinification (Araujo et al., 2016; Harsch et al., 2013). This research aimed to investigate the addition of different sources of sulfur in grape juice on formation of 3SH, 3SHA, 4SMP and benzene methanethiol (BM) in Sauvignon Blanc and Istrian Malvasia wines. Istrian Malvasia (Vitis vinifera L.) is a local grapevine variety which sometimes aromatically resemble on Sauvignon Blanc.Pressed grape juice was settled overnight and sampled from tank for microfermentations. Additions of different sulfur compounds in 700 mL of juice were performed just before yeast inoculation in four replicates each: (1) control – no addition, (2) addition of reduced glutathione (GSH) (20 and 50 mg/L), (3) addition of methionine (10 and 20 mg/L), (4) addition of cysteine (20 and 40 mg/L), (5) addition of elemental sulfur (5 and 10 mg/L) and (6) addition of  sodium hydrosulfide hydrate (1 and 10 mg/L). Fermentations were performed with Saccharomyces cerevisae (X5, Laffort, France) in 750 mL dark glass bottles at 18-20 °C. After fermentation, 50 mg/L SO2 was added, wines were settled, racked, and stored at -20 °C until analyses. Varietal thiols were analysed by GC-MS/MS (Schimadzu, TQ8050) using the modified method as described (Šuklje et al., 2013).Sauvignon Blanc wines with the addition of 10 mg/L NaSH·xH2O (which releases hydrogen sulfide) had significant higher concentration of 3SH and 3SHA compared to control and other treatments. Istrian Malvasia wines with the addition of 10 mg/L NaSH·xH2O had significant higher concentrations of 3SH but there were no significant differences in 3SHA.  The addition of elemental sulfur increased 3SH and 3SHA in both varieties, however not significantly. Addition of sulphur compounds in our study had no effect on concentration of 4MMP and BM in Sauvignon Blanc or Istrian Malvasia wines. On the other hand, the additions of GSH and cysteine resulted in significantly lower formation of 3SH and 3SHA in Sauvignon Blanc wines in comparison to control and other additions. The latter finding is compliant with the study on model wines by Alegre et al. (2019).Modifying sulfur source composition in grape juice showed some significant changes in concentration of 3SH in both Sauvignon Blanc and Istrian Malvasia wines.

References

Araujo D. L., Vannevel S., Buica A., Callerot S., Fedrizzi B., Kilmartin A. P., du Toit J. W. 2016. Indications of the prominent role of elemental sulfur in the formation of the varietal thiol 3-mercaptohexanol in Sauvignon blanc wine, Food Research International, 98, 79-86.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Vanzo Andreja1, Alenka Mihelčič1, Katja Šuklje1, Klemen Lisjak1

1 Agricultural Institute of Slovenia

Contact the author

Keywords

sulfur compounds, grape juice, varietal thiols, Sauvignon Blanc, Istrian Malvasia

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Updating the Winkler index: An analysis of Cabernet sauvignon in Napa Valley’s varied and changing climate

This study aims to create an updated, agile viticultural climate index (similar to the Winkler Index) by performing in-depth analyses of current and historical data from industry partners in several major winegrowing regions. The Winkler Index was developed in the early twentieth century based on analysis of various grape-growing regions in California. The index uses heat accumulation (i.e. Growing Degree Days) throughout the growing season to determine which grape varieties are best suited to each region. As viticultural regions are increasingly subject to the complexity and uncertainty of a changing climate, a more rigorous, agile model is needed to aid grape growers in determining which cultivars to plant where. For the first phase of this study, 21 industry partners throughout Napa Valley shared historical phenology, harvest, viticultural practice, and weather data related to their Cabernet sauvignon vineyard blocks. To complement this data, berry samples were collected throughout the 2021 growing season from 50 vineyard blocks located throughout 16 American Viticultural Areas that were then analyzed for basic berry chemistry and phenolics. These blocks have been mapped using a Geographic Information System (GIS), enabling analysis of altitude, vineyard row orientation, slope, and remotely sensed climate data. Sampling sites were also chosen based on their proximity to a weather station. By analyzing historical data from industry partners and data specifically collected for this study, it is possible to identify key parameters for further analysis. Initial results indicate extreme variability at a high spatial resolution not currently accounted for in modern viticultural climate indices and suggest that viticultural practices play a major role. Using the structure of data collection and analyses developed for the first phase, this project will soon be expanded to other wine regions globally, while continuing data collection in Napa Valley.

Modeling the suitability of Pinot Noir in Oregon’s Willamette Valley in a changing climate

Air temperature is the key driver of grapevine phenology and a significant environmental factor impacting yield and quality for a winegrape growing region. In this study the optimal downscaled CMIP5 ensemble for computing thegrowing season average temperature (GST) viticulture climate classification index was determined to spatially compute on a decadal basis predictions of the GST climate index and the grapevine sugar ripeness (GSR) model for Pinot Noir throughout the Willamette Valley (WV) American Viticultural Area (AVA). Forecasts for average temperature and a 220 g/L target sugar concentration level were computed using daily Localized Constructed Analogs (LOCA) downscaled CMIP5 historic and Representative Concentration Pathways (RCP) future climate projections of minimum and maximum daily temperature. We explore spatiotemporal trends of the GST climate classification index and Pinot Noir specific applications of the GSR phenology model for the WV AVA. Spatiotemporal computations of the GST climate index and Pinot Noir specific applications of the GSR model enable the opportunity to explore relationships between their computed values with one intent being to provide updated GST ranges that better align with current temperature-based modeling understanding of Pinot Noir grapevine phenology and the viticultural application of LOCA CMIP5 climate projections for the WV AVA. The Pinot Noir specific applications of the GSR model or the GST index with updated bounds indicate that the percent of the WV AVA area suitable for Pinot Noir production is currently at or near its peak value in the upper 80s to lower 90s of this century.

20-Year-Old data set: scion x rootstock x climate, relationships. Effects on phenology and sugar dynamics

Global warming is one of the biggest environmental, social, and economic threats. In the Douro Valley, change to the climate are expected in the coming years, namely an increase in average temperature and a decrease in annual precipitation. Since vine cultivation is extremely vulnerable and influenced by the climate, these changes are likely to have negative effects on the production and quality of wine.
Adaptation is a major challenge facing the viticulture sector where the choice of plant material plays an important role, particularly the rootstock as it is a driver for adaptation with a wide range of effects, the most important being phylloxera, nematode and salt, tolerance to drought and a complex set of interactions in the grafted plant.
In an experimental vineyard, established in the Douro Region in 1997, with four randomized blocs, with five varieties, Touriga Nacional, Tinta Barroca, Touriga Franca and Tinta Roriz, grafted in four rootstocks, Rupestris du Lot, R110, 196-17C, R99 and 1103P, data was collected consecutively over 20 years (2001-2020). Phenological observations were made two to three times a week, following established criteria, to determine the average dates of budbreak, flowering and veraison. During maturation, weekly berry samples were taken to study the dynamics of sugar accumulation, amongst other parameters. Climate data was collected from a weather station located near the vineyard parcel, with data classified through several climatic indices.
The results achieved show a very low coefficient of variations in the average date of the phenophases and an important contribution from the rootstock in the dynamic of the phenology, allowing a delay in the cycle of up to10-12 days for the different combinations. The Principal Component Analysis performed, evaluating trends in the physical-chemical parameters, highlighted the effect of the climate and rootstock on fruit quality by grape varieties.

Sustaining wine identity through intra-varietal diversification

With contemporary climate change, cultivated Vitis vinifera L. is at risk as climate is a critical component in defining ecologically fitted plant materiel. While winegrowers can draw on the rich diversity among grapevine varieties to limit expected impacts (Morales-Castilla et al., 2020), replacing a signature variety that has created a sense of local distinctiveness may lead to several challenges. In order to sustain wine identity in uncertain climate outcomes, the study of intra-varietal diversity is important to reflect the adaptive and evolutionary potential of current cultivated varieties. The aim of this ongoing study is to understand to what extent can intra-varietal diversity be a climate change adaptation solution. With a focus on early (Sauvignon blanc, Riesling, Grolleau, Pinot noir) to moderate late (Chenin, Petit Verdot, Cabernet franc) ripening varieties, data was collected for flowering and veraison for the various studied accessions (from conservatory plots) and clones. For these phenological growing stages, heat requirements were established using nearby weather stations (adapted from the GFV model, Parker et al., 2013) and model performances were verified. Climate change projections were then integrated to predict the future behaviour of the intra-varietal diversity. Study findings highlight the strong phenotypic diversity of studied varieties and the importance of diversification to enhance climate change resilience. While model performances may require improvements, this study is the first step towards quantifying heat requirements of different clones and how they can provide adaptation solutions for winegrowers to sustain local wine identity in a global changing climate. As genetic diversity is an ongoing process through point mutations and epigenetic adaptations, perspective work is to explore clonal data from a wide variety of geographic locations.

Use of a new, miniaturized, low-cost spectral sensor to estimate and map the vineyard water status from a mobile 

Optimizing the use of water and improving irrigation strategies has become increasingly important in most winegrowing countries due to the consequences of climate change, which are leading to more frequent droughts, heat waves, or alteration of precipitation patterns. Optimized irrigation scheduling can only be based on a reliable knowledge of the vineyard water status.

In this context, this work aims at the development of a novel methodology, using a contactless, miniaturized, low-cost NIR spectral tool to monitor (on-the-go) the vineyard water status variability. On-the-go spectral measurements were acquired in the vineyard using a NIR micro spectrometer, operating in the 900–1900 nm spectral range, from a ground vehicle moving at 3 km/h. Spectral measurements were collected on the northeast side of the canopy across four different dates (July 8th, 14th, 21st and August 12th) during 2021 season in a commercial vineyard (3 ha). Grapevines of Vitis vinifera L. Graciano planted on a VSP trellis were monitored at solar noon using stem water potential (Ψs) as reference indicators of plant water status. In total, 108 measurements of Ψs were taken (27 vines per date).

Calibration and prediction models were performed using Partial Least Squares (PLS) regression. The best prediction models for grapevine water status yielded a determination coefficient of cross-validation (r2cv) of 0.67 and a root mean square error of cross-validation (RMSEcv) of 0.131 MPa. This predictive model was employed to map the spatial variability of the vineyard water status and provided useful, practical information towards the implementation of appropriate irrigation strategies. The outcomes presented in this work show the great potential of this low-cost methodology to assess the vineyard stem water potential and its spatial variability in a commercial vineyard.