IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Antifungal and Laccase-Suppressing Activity of Phenolic Compounds and Their Oxidation Products on Grey Mold-Fungus Botrytis cinerea

Antifungal and Laccase-Suppressing Activity of Phenolic Compounds and Their Oxidation Products on Grey Mold-Fungus Botrytis cinerea

Abstract

Botrytis cinerea causes grey mold that results in severe problems for wine makers worldwide. Infected grapes lead to quality deterioration including formation of off-flavors or browning. The latter is caused by the enzyme laccase which is capable of oxidizing a wide range of phenolic compounds. Since the use of conventional pesticides is associated with many concerns of consumers and authorities regarding environmental and health related issues and may result in fungicide resistance, the development of green alternatives is gaining more attention. Phenolic compounds like ferulic acid or stilbens which serve as phytoalexins have shown promising results in terms of growth-inhibitory effects against B. cinerea. The present study focuses on further investigations of the antifungal and laccase-suppressing effects of these phenols and especially their laccase-derived oxidation products using the syringaldazine-assay for determination of the laccase activity. The results revealed that they can be considered as a sustainable alternative to synthetic fungicides for Botrytis cinerea management.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Umberath Kim Marie1, Schieber Andreas1 and Weber Fabian1

1University of Bonn, Institute of Nutritional and Food Sciences, Molecular Food Technology

Contact the author

Keywords

Botrytis cinerea, laccase, phenolic compounds, oxidation products, antifungal activity

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Assessment of O2 consumption, a new tool to select bioprotection yeast strains

Reduction of sulfur dioxide during winemaking is a request from the wine industry. To replace sulfur dioxide, various alternatives exist, including bioprotection by yeast inoculation. This practice consists in adding non-Saccharomyces yeasts directly on the grapes or must.

La producción vitivinícola como fuente de impactos positivos en el medio seminatural

Wine is at risk of being labelled as a dangerous health product, based on studies that focus on its alcohol content. However, multiple studies suggest that moderate consumption is healthy. Changing the focus from health impacts to the environmental and socioeconomic impacts that wine companies cause, what can be said?

Environmental influence on grape phenolic and aromatic compounds in a Nebbiolo selection (Vitis vinifera L.)

Nebbiolo (Vitis vinifera L.) is one of the most important wine red cultivar of North-west Italy. A better understanding of the complex relations among grape aromatic and phenolic maturity and environmental factors may strongly contribute to the improvement of the quality of Nebbiolo wines.

Exploring microbial interactions between Saccharomyces cerevisiae and non-Saccharomyces yeast starters in vinification

Winemaking is a complex microbial process involving the co-existence and interactions of various microorganisms [1].

Technological possibilities of grape marc cell walls as wine fining agent. Effect on wine phenolic composition

Fining is a technique that is used to remove unwanted wine components that affect clarification, astringency, color, bitterness, and aroma. Fining involves the addition of adsorptive or reactive material in order to reduce or eliminate the presence of certain less desirable wine components and to ensure that a wine remains in a particular stable state for a given period of time Recently concerns have been raised about the addition of animal proteins, such as gelatin, to wine due to the disease known as bovine spongiform encephalopathy (Mad Cow disease). Although the origin of gelatins has been moved to porcine, winemakers are asking for substitute products with properties and application protocols similar to the traditional animal-derived ones, making the use of plant-derived proteins in fining a practically viable possibility. As a consequence, various fining agents derived from plants have been proposed, including proteins from cereals, legumes, and potato.