IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Fast, and full microbiological wine analysis using triple cellular staining.

Fast, and full microbiological wine analysis using triple cellular staining.

Abstract

We propose here a brand new large routine microbiological analysis method intended for oenology, in flow cytometry, using high performance equipment and triple selective cell staining, activated by fluorescence. The results and practical applications of the method are presented: Brettanomyces (Dekkera) Monitoring, fermentations monitoring, bottling and enological practices monitoring.The method allow a complete new microbiological tool for wine industry.The method has been accredited ISO 17025 in our laboratories.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Dubernet Matthieu1, Husset Mélanie1, Adler Sophie1, Lefebvre Cyril1, Guichard Perrine1, Hernandez Fanny1 and Paricaud Tatiana1

1Laboratoires Dubernet

Contact the author

Keywords

Wine microbiology, cytometry, brettanomyces, saccharomyces, bacteria

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Volatile and phenolic profiles of wines closed with different stoppers and stored for 30 months

The aim of this study was to evaluate the volatile and phenolic profiles of three red and one rosé wines stored in bottles for 30 months. Four wines were provided by a winery located in South Tyrol

Impact of GoLo technology on the aroma profile of red and white wines after total and partial dealcoholisation

Wine dealcoholisation has been practised since the early 1900s and has gained importance due to climate change
and shifting consumer preferences for lower-alcohol beverages. Rising temperatures are accelerating grape
ripening, increasing sugar content and, consequently, raising the alcohol strength of wines.

IMPACT OF CLIMATIC ZONES ON THE AROMATIC PROFILE OF CORVINA WINES IN THE VALPOLICELLA REGION

In Italy, in the past two decades, the rate of temperature increases (0.0369 °C per year) was slightly higher compared to the world average (0.0313 °C per year). It has also been indicated that the number and intensity of heat waves have increased considerably in the last decades. (IEA, 2022). Viticultural zones can be classified with climatic indexes. Huglin’s index (HI) considers the temperature in a definite area and has been considered as reliable to evaluate the thermal suitability for winegrape production (Zhang et al., 2023).

Microclimatic differences in fruit zone of vineyards on different elevations of ‘nagy-eged hill’ in eger wine region, Hungary

The Bull’s Blood of Eger (‘Egri Bikavér’) is one of the most reputed red wines in Hungary and abroad, produced in the Northeastern part of the country.

Population-wide diversity study in Lachancea thermotolerans highlights superior starters for winemaking

Grapes from warm(ing) climates often contain excessive sugars but lack acidity. This can lead to highly alcoholic wines with compromised stability and balance. The yeast Lachancea thermotolerans can ameliorate such wines due to its metabolic peculiarity – partial fermentation of sugars to lactic acid. This study aimed to elucidate the population-wide diversity in L. thermotolerans, whilst selecting superior strains for wine sector. An extensive collection of isolates (~200) sourced from different habitats worldwide was first genotyped on 14 microsatellite loci. This revealed differentiation of L. thermotolerans genetic groups based on the isolation substrate and geography. The 94 genotyped strains were then characterised in Vitis vinifera cv. Chardonnay fermentations.