IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Fast, and full microbiological wine analysis using triple cellular staining.

Fast, and full microbiological wine analysis using triple cellular staining.

Abstract

We propose here a brand new large routine microbiological analysis method intended for oenology, in flow cytometry, using high performance equipment and triple selective cell staining, activated by fluorescence. The results and practical applications of the method are presented: Brettanomyces (Dekkera) Monitoring, fermentations monitoring, bottling and enological practices monitoring.The method allow a complete new microbiological tool for wine industry.The method has been accredited ISO 17025 in our laboratories.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Dubernet Matthieu1, Husset Mélanie1, Adler Sophie1, Lefebvre Cyril1, Guichard Perrine1, Hernandez Fanny1 and Paricaud Tatiana1

1Laboratoires Dubernet

Contact the author

Keywords

Wine microbiology, cytometry, brettanomyces, saccharomyces, bacteria

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

EXPLORING THE INFLUENCE OF S. CEREVISIAE MANNOPROTEINS ON WINE ASTRINGENCY AND THE IMPACT OF THEIR POLYSACCHARIDE STRUCTURE

Mannoproteins (MPs) are proteoglycans from the outmost layer of yeast cell walls released into wine during alcoholic fermentation and ageing on lees processes. The use of commercial preparations of mannoproteins as additives to improve wine stability with regards to the crystallization of tartaric salts and to prevent protein haze in the case of white and rosé wines is authorized by the OIV. Regarding red wines and polyphenols, mannoproteins are described as able to improve their colloidal stability and modulate the astringent effect of condensed tannins. The latter interact with salivary proteins forming insoluble aggregates that cause a loss of lubrication in the mouth and promote a drying and puckering sensation. However, neither the interaction mechanisms involved in mannoproteins capacity to impact astringency nor the structure-function relationships related to this property are fully understood.

Analyse du rôle du terroir dans la définition d’une appellation d’origine

In France, the definition of appellations of origins is entrusted to the Institut National des Appellations d’Origine. (‘NAO). With the increase in price of appellations of origin vine­yards and considering the interests at stake, Institut National des Appellations d’Origine and the Institut National de Recherche Agronomique (INRA) established a work group in 1993 in order to study the “terroir-wine” relationship as precisely as possible, taking into account the knowledge acquired by researchers of the INRA and the experience in the field of the agents of the INAO.

2018 updates on the agronomic performances of fungus resistant wine grapes in Trentino (Italy)

On the market there are several wine grapes which are tolerant to the main fungal diseases. These varieties, commonly defined “resistant”, were developed in the grapevine breeding programs carried out mainly in Germany, France, Hungary and Italy. Some of these cultivars have been included in the national catalogues of wine grape varieties and have sometimes been allowed for specific kinds of wine. The VEVIR project, aimed at the enological evaluation of resistant vines, involves 33 cultivars achieved at the State Institute for Viticulture Freiburg in Germany, the Research Institute of Viticulture and Enology Pecs in Hungary and the Fondazione Edmund Mach S. Michele all’Adige (FEM) in Italy.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Potential use of the yeast Starmerella bacillaris as a sustainable biocontrol agent against gray mold disease in viticulture

Pest biocontrol strategies are gaining attention as eco-friendly alternatives to the use of synthetic pesticides, including in viticulture.