IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Effect of the presence of anthocyanins on the interaction between wine phenolic compounds and high molecular weight salivary proteins

Effect of the presence of anthocyanins on the interaction between wine phenolic compounds and high molecular weight salivary proteins

Abstract

As a result of climate change consequences, there is a gap between the times at which the grapes reach the phenolic and the technology maturities. As a consequence, the wine sensory properties are affected and, among them, astringency, one of the most important organoleptic attributes for red wine quality. A balanced astringency is essential for quality wines, since when this sensation is perceived with high intensity, it is considered as unpleasant by consumers. The main mechanism described for the astringency development is the interaction of wine phenolic compounds, such as flavanols and flavonols, with salivary proline-rich proteins (PRPs), forming protein-flavanol complexes that can precipitate, resulting in a loss of lubrication in the oral cavity.Although PRPs are the main proteins studied to explain astringency, there are other types of proteins in saliva, such as mucins that are high molecular weight glycoproteins representing the main proteins in the salivary proteome. It has been reported that mucins can interact with wine flavanols, which could compromise the lubricating functions of mucins, so these proteins may play an important role in astringency sensation.1 Thus, it is important to go deeper into the study of the interactions of these proteins with wine phenolic compounds and the factors that could affect them to get new insight about the mechanisms of astringency sensation.Flavanols and flavonols are also involved in the stabilization of colored forms of malvidin-3-O-glucoside (Mv) through copigmentation effect. Moreover, it has been reported that some flavanol-anthocyanin mixtures present a synergic effect toward the interaction with PRPs when compared to individual polyphenols.2 Hence, the main aim of this work is to assess if the interaction between flavanols and flavonols and high molecular weight proteins is affected due to the involvement of these phenolic compounds in the copigmentation effect. To do this, ternary interactions involving Mv, two individual flavanols (catechin and epicatechin) and/or the flavonol quercetin-3-O-glucoside with mucin from bovine submaxillary glands have been studied by isothermal titration calorimetry (ITC).ITC results show that the studied interactions are driven by both hydrophobic interactions and H-bonds. Results show that mucin interact with the wine phenolic compounds assayed, confirming the possible role of mucins in astringency sensation due to the effect that this interaction may have on lubricating functions of these proteins. Moreover, the presence of anthocyanins in the mixtures affects the interaction between mucins and the phenolic compounds studied, which points out that anthocyanins could play an indirect role on astringency development ant that the whole wine phenolic composition should be considered when astringency sensation is studied.

References

(1) Brandão, E. et al. Molecular study of mucin-procyanidin interaction by fluorescence quenching and saturation transfer difference (STD)-NMR. Food Chem. 2017, 228, 427-434.
(2) Soares, S. et al. Effect of malvidin-3-glucoside and epicatechin interaction on Ttheir ability to interact with salivary proline-rich proteins. Food Chem. 2019, 276, 33–42.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Torres-Rochera Bárbara1, García-Estévez Ignacio1 and Esribano-Bailón Mará Teresa1

1Department of Analytical Chemistry, Nutrition and Food Sciences, Universidad de Salamanca

Contact the author

Keywords

astringency, copigmentation, wine phenolic compounds and ITC

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Biodiversity in the vineyard agroecosystem: exploring systemic approaches

Biodiversity conservation and restoration are essential for guarantee the provision of ecosystem services associated to vineyard agroecosystem such as climate regulation trough carbon sequestration and control of pests and diseases. Most of published research dealing with the complexity of the vineyard agroecosystems emphasizes the necessity of innovative approaches, including the integration of information at different temporal and spatial scales and development of systemic analysis based on modelling. A biodiversity survey was conducted in the Franciacorta wine-growing area (Lombardy, Italy), one of the most important Italian wine-growing regions for sparkling wine production, considering a portion of the territory of 112 ha. The area was divided into several Environmental Units (EUs), defined as a whole vineyard or portion of vineyard homogenous in terms of four agronomic characteristics: planting year, planting density, cultivar, and training system. In each EU a set of compartments was identified and characterised by specific variables. The compartments are meteorology, morphology (altitude, slope, aspect, row orientation, and solar irradiance), ecological infrastructures and management. The landscape surrounding EU was also characterised in terms of land-use in a buffer zone of 500 m. For each component a specific methodology was identified and applied. Different statistical approaches were used to evaluate the method to integrate the information related to different compartments within the EU and related to the buffer zone. These approaches were also preliminarily evaluated for their ability to describe the contribution of biodiversity and landscape components to ecosystem services. This methodological exploration provides useful indication for the development of a fully systemic approach to structural and functional biodiversity in vineyard agroecosystems, contributing to promote a multifunctional perspective for the all wine-growing sector.

Effect of multi-level and multi-scale spectral data source on vineyard state assessment

Currently, the main goal of agriculture is to promote the resilience of agricultural systems in a sustainable way through the improvement of use efficiency of farm resources, increasing crop yield and quality under climate change conditions. This last is expected to drastically modify plant growth, with possible negative effects, especially in arid and semi-arid regions of Europe on the viticultural sector. In this context, the monitoring of spatial behavior of grapevine during the growing season represents an opportunity to improve the plant management, winegrowers’ incomes, and to preserve the environmental health, but it has additional costs for the farmer. Nowadays, UAS equipped with a VIS-NIR multispectral camera (blue, green, red, red-edge, and NIR) represents a good and relatively cheap solution to assess plant status spatial information (by means of a limited set of spectral vegetation indices), representing important support in precision agriculture management during the growing season. While differences between UAS-based multispectral imagery and point-based spectroscopy are well discussed in the literature, their impact on plant status estimation by vegetation indices is not completely investigated in depth. The aim of this study was to assess the performance level of UAS-based multispectral (5 bands across 450-800nm spectral region with a spatial resolution of 5cm) imagery, reconstructed high-resolution satellite (Sentinel-2A) multispectral imagery (13 bands across 400-2500 nm with spatial resolution of <2 m) through Convolutional Neural Network (CNN) approach, and point-based field spectroscopy (collecting 600 wavelengths across 400-1000 nm spectral region with a surface footprint of 1-2 cm) in a plant status estimation application, and then, using Bayesian regularization artificial neural network for leaf chlorophyll content (LCC) and plant water status (LWP) prediction. The test site is a Greco vineyard of southern Italy, where detailed and precise records on soil and atmosphere systems, in-vivo plant monitoring of eco-physiological parameters have been conducted.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Is wine terroir a valid concept under a changing climate?

The OIV[i] defines terroir as a concept referring to an area in which collective knowledge of the interactions between the physical and biological environment (soil, topography, climate, landscape characteristics and biodiversity features) and vitivinicultural practices develops, providing distinctive wine characteristics. Those are perceptible in the taste of wine, which drives consumer preference and, therefore, wine’s value in the marketplace. Geographical indications (GI) are recognized regulatory constructs formalizing and protecting the nexus between wine taste and the terroir generating it. Despite considering updates, GIs do not consider the nexus as a dynamic one and do not anticipate change, namely of climate. Being climate a fundamental feature of terroir, it strongly impacts wine characteristics, such as taste. According to IPCC[ii], many widespread, rapid and unprecedented changes of climate occurred, some being irreversible over hundreds to thousands of years. Climatic shifts and atmospheric-driven extreme events have been widely reported worldwide. Recent climatic trends are projected to strengthen in upcoming decades, whereas extremes are expected to increase in frequency and intensity, forcing wines away from GI definitions. Geographical shifts of viticultural suitability are projected, often moving into regions and countries different from current ones. Some authors propose adaptation in viticulture, winemaking and product innovation. We show evidence of climate changing wine characteristics in the Douro valley, home of 270-year-old Port GI. We discuss herein resist or adapt stances for when climate changes the nexus between terroir and wine characteristics. Using the MED-GOLD[iii] dashboard, a tool allowing for easy visual navigation of past and future climates, we demonstrate how policymakers can identify future moments, throughout the 21st century under different emission scenarios, when GI specifications will likely need updates (e.g., boundaries, varieties) to reduce climate-change impacts.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.