WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 3 - WAC - Posters 9 Bio-acidification of wines by Lachancea thermotolerans

Bio-acidification of wines by Lachancea thermotolerans

Abstract

Insufficient acidity in grapes from warm climates/vintages is commonly corrected through addition of tartaric acid during vinification, and less so with other organic acids. An alternative approach involves bio-acidification with the yeast Lachancea thermotolerans (LT) via lactic acid production during fermentation. Our work first elucidated the genetic (~200) and phenotypic (~100) diversity of LT strains, and then tested the performance of their subset in co-cultures with Saccharomyces cerevisiae (SC). In pure and mixed cultures alike, the modulation of acidity and other compositional parameters of wines depended on the LT strain, with either comparable or significant acidification relative to the SC control. An LT strain with exceptional bio-acidifying properties was selected, capable of lowering wine pH by ~0.5 units, and further characterised across a range of oenological conditions.

Our follow-up study aimed to i) compare the profiles of bio-acidified LT wines and acid-adjusted SC wines, and ii) evaluate the use of LT wines as blending components. For this purpose, high sugar/pH Merlot grapes were fermented with a sequential culture of LT and SC, and an SC monoculture. The aliquots of the SC control (pH 4) were acidified with either tartaric or lactic acid to the pH of the LT wine (pH 3.6), and the initial wines also blended in three proportions (1:3, 1:1, 3:1). Chemical analysis revealed major differences in a range of chemical parameters of wines (e.g. ethanol content, acidity parameters, volatile compounds, amino acids).  The compositional modulations were reflected in the sensory profiles of wines, as confirmed via ‘Rate-All-That-Apply’ evaluation by wine experts (n=30). Sensory profiles of the bio-acidified LT wine and the lactic acid-adjusted SC wine were similar, and contrasting to the tartaric acid-adjusted SC wine. Despite an identical initial matrix, lactic acid-adjusted SC wine had higher ‘red fruit’ flavour, and lower ‘hotness’, ‘bitterness’ and ‘body’ relative to tartaric acid-adjusted wine. This was driven by differences in ‘acidity’ perception, affected by titratable acidity (rather than pH) of wines. An inhibition of Brettanomyces bruxellensis growth was also observed in the bio-acidified LT wine and the lactic-acid adjusted SC wine. The profiles of blends were modulated depending on the proportion of the bio-acidified wine, thus highlighting the potential of this approach to boost ‘freshness’ and differentiate wine styles.

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Ana, Hranilovic, Marina Bely, Isabelle Masneuf-Pomarede, Joana Coulon, Warren Albertin, Vladimir Jiranek

Presenting author

Ana, Hranilovic – Department of Wine Science, The University of Adelaide, Australia

Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | BioLaffort, Floirac, France | Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | Department of Wine Science, The University of Adelaide, Australia,

Contact the author

Keywords

non-Saccharomyces yeasts – Lachancea thermotolerans – wine acidification – volatile composition – RATA sensory profiling

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Downscaling of remote sensing time series: thermal zone classification approach in Gironde region

In viticulture, the challenges of local climate modelling are multiple: taking into account the local environment, fine temporal and spatial scales, reliable time series of climate data, ease of implementation and reproducibility of the method. At the local scale, recent studies have demonstrated the contribution of spatialization methods for ground-based climate observation data considering topographic factors such as altitude, slope, aspect, and geographic coordinates (Le Roux et al, 2017; De Rességuier et al, 2020). However, these studies have shown questions in terms of the reproducibility and sustainability of this type of climate study. In this context, we evaluated the potential of MODIS thermal satellite images validated with ground-based climate data (Morin et al, 2020). Previous studies have been encouraging, but questions remain to be explored at the regional scale, particularly in the dynamics of the massive use of bioclimatic indices to classify the climate of wine regions. The results at the local scale were encouraging, but this approach was tested in the current study at the regional scale. Several objectives were set: 1) to evaluate the downscaling method for land surface temperature time series, 2) to identify regional thermal structure variations. We used weekly minimum and maximum surface temperature time series acquired by MODIS satellites at a spatial resolution of 1000 m and downscaled at 500 m using topographical variables. Two types of analyses were performed:

Inhibition of Oenococcus oeni during alcoholic fermentation by a selected Lactiplantibacillus plantarum strain

The use of selected cultures of the species Lactiplantibacillus plantarum in Oenology has grown in prominence in recent years. While initial applications of this species centred very much around malolactic fermentation (MLF), there is strong evidence to show that certain strains can be harnessed for their bio-protective effects. Unwanted spontaneous MLF during alcoholic fermentation (AF), driven by rogue Oenococcus oeni, is a winemaking deviation that is very difficult to manage when it occurs. This work set out to determine the efficacy of one particular strain of Lactiplantibacillus plantarum(Viniflora® NoVA™ Protect), against this problem in Cabernet Sauvignon must. The work was carried out at commercial scale and in a winery environment and compared the bio-protective culture with the more traditional approach of reducing must pH by the addition of tartaric acid. The combination of both was also investigated. The concentration of both Oenococcus oeni and Lactiplantibacillus plantarum was determined using qPCR. The adventitious Oenococcus oeni showed the most growth during AF in the control wine, whereas in the wines treated with Lactiplantibacillus plantarum a bacteriostatic effect against this species was observed. This effect was comparable to the wines treated with tartaric acid. This has particular commercial relevance for controlling the flora in musts with high pH, or when the addition of tartaric acid is either not permitted or is prohibitive for other reasons.

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

How does aromatic composition of red wines, resulting from varieties adapted to climate change, modulate fruity aroma?

One of the major issues for the wine sector is the impact of climate change linked to the increasing temperatures which affects physicochemical parameters of the grape varieties planted in Bordeaux vineyard and consequently, the quality of wine. In some varietals, the attenuation of their fresh fruity character is accompanied by the accentuation of dried-fruit notes [1]. As a new adaptive strategy on climate change, some winegrowers have initiated changes in the Bordeaux blend of vine varieties [2]. This study intends to explore the fruitiness in wines produced from grape varieties adapted to the future climate of Bordeaux. 10 commercial single–varietal wines from 2018 vintage made from the main grape varieties in the Bordeaux region (Cabernet franc, Cabernet-Sauvignon and Merlot) as well as from indigenous grape varieties from the Mediterranean basin, such as Cyprus (Yiannoudin), France (Syrah), Greece (Agiorgitiko and Xinomavro), Portugal (Touriga Nacional) and Spain (Garnacha and Tempranillo), were selected among 19 samples using sensory descriptive analyses. Both sensory and instrumental analyses were coupled, to investigate their fruity aroma expression. For sensory analysis, samples were prepared from wine, using a semi preparative HPLC method which preserves wine aroma and isolates fruity characteristics in 25 specific fractions [3,4]. Fractions of interest with intense fruity aromas were sensorially selected for each wine by a trained panel and mixed with ethanol and microfiltered water to obtain fruity aromatic reconstitutions (FAR) [5]. A free sorting task was applied to categorize FAR according to their similarities or dissimilarities, and different clusters were highlighted. Instrumental analysis of the different FAR and wines demonstrated variations in their molecular composition. Results obtained from sensory and gas chromatography analysis enrich the knowledge of the fruity expression of red wines from “new” grape varieties opening up new perspectives in wine technology, including blending, thus providing new tools for producers.

Upscaling the integrated terroir zoning through digital soil mapping: a case study in the Designation of Origin Campo de Borja

homogeneous zones by intersecting several partial zonings of major factors that influence vineyard growth. Each of them follows specific process from their corresponding disciplines. Soil zoning specifically refers to a Soil Resource Inventory map that has traditionally been generated by conventional soil mapping methods. These methods have shortcomings in reaching fine cartographic and categorical details and involve significant expenses, which undermines their applicability. A new framework named Digital Soil Mapping has introduced quantitative models by statistical techniques to establish soil-landscape relationships and is able to provide intensive scale cartography.

In the present study, a microzoning at 1:10.000 scale is generated from an initial zoning, where the conventional soil map with polytaxic map units is replaced by a new one from digital techniques that disaggregates them. The comparison between the zonings considers a quantitative evaluation of capability for each Homogeneous Terroir Unit by means of the Viticultural Quality Index and its categorization based on its distribution by map. The spatial intersection of both maps gives rise to a confusion matrix in which the flows of class variations after the substitution are assessed.

The results show a five-fold increase in the number of Homogeneous Terroir Units identified and a larger differentiation among them, evidenced by a wider range in the capability index distribution. Both elements are accompanied by an increase in the detection of areas of higher potential within previously undervalued uniform zones.These features are a direct effect of the improvements brought by Digital Soil Mapping techniques and would verify the advantages of their implementation in the Integrated Terroir zoning. Eventually, such new highly detailed terroir units would benefit precision viticulture and sustainable management practices.