WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 3 - WAC - Posters 9 Bio-acidification of wines by Lachancea thermotolerans

Bio-acidification of wines by Lachancea thermotolerans

Abstract

Insufficient acidity in grapes from warm climates/vintages is commonly corrected through addition of tartaric acid during vinification, and less so with other organic acids. An alternative approach involves bio-acidification with the yeast Lachancea thermotolerans (LT) via lactic acid production during fermentation. Our work first elucidated the genetic (~200) and phenotypic (~100) diversity of LT strains, and then tested the performance of their subset in co-cultures with Saccharomyces cerevisiae (SC). In pure and mixed cultures alike, the modulation of acidity and other compositional parameters of wines depended on the LT strain, with either comparable or significant acidification relative to the SC control. An LT strain with exceptional bio-acidifying properties was selected, capable of lowering wine pH by ~0.5 units, and further characterised across a range of oenological conditions.

Our follow-up study aimed to i) compare the profiles of bio-acidified LT wines and acid-adjusted SC wines, and ii) evaluate the use of LT wines as blending components. For this purpose, high sugar/pH Merlot grapes were fermented with a sequential culture of LT and SC, and an SC monoculture. The aliquots of the SC control (pH 4) were acidified with either tartaric or lactic acid to the pH of the LT wine (pH 3.6), and the initial wines also blended in three proportions (1:3, 1:1, 3:1). Chemical analysis revealed major differences in a range of chemical parameters of wines (e.g. ethanol content, acidity parameters, volatile compounds, amino acids).  The compositional modulations were reflected in the sensory profiles of wines, as confirmed via ‘Rate-All-That-Apply’ evaluation by wine experts (n=30). Sensory profiles of the bio-acidified LT wine and the lactic acid-adjusted SC wine were similar, and contrasting to the tartaric acid-adjusted SC wine. Despite an identical initial matrix, lactic acid-adjusted SC wine had higher ‘red fruit’ flavour, and lower ‘hotness’, ‘bitterness’ and ‘body’ relative to tartaric acid-adjusted wine. This was driven by differences in ‘acidity’ perception, affected by titratable acidity (rather than pH) of wines. An inhibition of Brettanomyces bruxellensis growth was also observed in the bio-acidified LT wine and the lactic-acid adjusted SC wine. The profiles of blends were modulated depending on the proportion of the bio-acidified wine, thus highlighting the potential of this approach to boost ‘freshness’ and differentiate wine styles.

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Ana, Hranilovic, Marina Bely, Isabelle Masneuf-Pomarede, Joana Coulon, Warren Albertin, Vladimir Jiranek

Presenting author

Ana, Hranilovic – Department of Wine Science, The University of Adelaide, Australia

Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | BioLaffort, Floirac, France | Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | Department of Wine Science, The University of Adelaide, Australia,

Contact the author

Keywords

non-Saccharomyces yeasts – Lachancea thermotolerans – wine acidification – volatile composition – RATA sensory profiling

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

The interplay between grape ripening and weather anomalies – A modeling exercise

Current climate change is increasing inter- and intra-annual variability in atmospheric conditions leading to grapevine phenological shifts as well altered grape ripening and composition at ripeness. This study aims to (i) detect weather anomalies within a long-term time series, (ii) model grape ripening revealing altered traits in time to target specific ripeness thresholds for four Vitis vinifera cultivars, and (iii) establish empirical relationships between ripening and weather anomalies with forecasting purposes. The Day of the Year (DOY) to reach specific grape ripeness targets was determined from time series of sugar concentrations, total acidity and pH collected from a private company in the period 2009-2021 in North-Eastern Italy. Non-linear models for the DOY to reach the specified ripeness thresholds were assessed for model efficiency (EF) and error of prediction (RMSE) in four grapevine cultivars (Merlot, Cabernet Sauvignon, Glera and Garganega). For each vintage and cultivar, advances or delays in DOY to target specified ripeness thresholds were assessed with respect to the average ripening dynamics. Long-term meteorological series monitored at ground weather station by means of hourly air temperature and rainfall data were analyzed. Climate statistics were obtained and for each time period (month, bimester, quarter and year) weather anomalies were identified. A linear regression analysis was performed to assess a possible correlation that may exist between ripening and weather anomalies. For each cultivar, ripeness advances or delays expressed in number of days to target the specific ripening threshold were assessed in relation to registered weather anomalies and the specific reference time period in the vintage. Precipitation of the warmest month and spring quarter are key to understanding the effect of climate change on sugar ripeness. Minimum temperatures of May-June bimester and maximum temperatures of spring quarter best correlate with altered total acidity evolution and pH increment during the ripening process, respectively.

Elevational range shifts of mountain vineyards: Recent dynamics in response to a warming climate

Increasing temperatures worldwide are expected to cause a change in spatial distribution of plant species along elevational gradients and there are already observable shifts to higher elevations as a consequence of climate change for many species. Not only naturally growing plants, but also agricultural cultivations are subject to the effects of climate change, as the type of cultivation and the economic viability depends largely on the prevailing climatic conditions. A shift to higher elevations therefore represents a viable adaptation strategy to climate change, as higher elevations are characterized by lower temperatures. This is especially important in the case of viticulture because a certain wine-style can only be achieved under very specific climatic conditions. Although there are several studies investigating climatic suitability within winegrowing regions or longitudinal shifts of winegrowing areas, little is known about how fast vineyards move to higher elevations, which may represent a viable strategy for winegrowers to maintain growing conditions and thus wine-style, despite the effects of climate change. We therefore investigated the change in the spatial distribution of vineyards along an elevational gradient over the past 20 years in the mountainous wine-growing region of Alto Adige (Italy). A dataset containing information about location and planting year of more than 26000 vineyard parcels and 30 varieties was used to perform this analysis. Preliminary results suggest that there has been a shift to higher elevations for vineyards in general (from formerly 700m to currently 850 m a.s.l., with extreme sites reaching 1200 m a.s.l.), but also that this development has not been uniform across different varieties and products (i.e. vitis vinifera vs hybrid varieties and still vssparkling wines). This is important for climate change adaptation as well as for rural development. Mountain areas, especially at mid to high elevations, are often characterized by severe land abandonment which can be avoided to some degree if economically viable and sustainable land management strategies are available.

Amino nitrogen content in grapes: the impact of crop limitation

As an essential element for grapevine development and yield, nitrogen is also involved in the winemaking process and largely affects wine composition. Grape must amino nitrogen deficiency affects the alcoholic fermentation kinetics and alters the development of wine aroma precursors. It is therefore essential to control and optimize nitrogen use efficiency by the plant to guarantee suitable grape nitrogen composition at harvest. Understanding the impact of environmental conditions and cultural practices on the plant nitrogen metabolism would allow us to better orientate our technical choices with the objective of quality and sustainability (less inputs, higher efficiency). This trial focuses on the impact of crop limitation – that is a common practice in European viticulture – on nitrogen distribution in the plant and particularly on grape nitrogen composition. A wide gradient of crop load was set up in a homogeneous plot of Chasselas (Vitis vinifera) in the experimental vineyard of Agroscope, Switzerland. Dry weight and nitrogen dynamics were monitored in the roots, trunk, canopy and grapes, during two consecutive years, using a 15N-labeling method. Grape amino nitrogen content was assessed in both years, at veraison and at harvest. The close relationship between fruits and roots in the maintenance of plant nitrogen balance was highlighted. Interestingly, grape nitrogen concentration remained unchanged regardless of crop load to the detriment of the growth and nitrogen content of the roots. Meanwhile, the size and the nitrogen concentration of the canopy were not affected. Leaf gas exchange rates were reduced in response to lower yield conditions, reducing carbon and nitrogen assimilation and increasing intrinsic water use efficiency. The must amino nitrogen profiles could be discriminated as a function of crop load. These findings demonstrate the impact of plant balance on grape nitrogen composition and contribute to the improvement of predictive models and sustainable cultural practices in perennial crops.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…