WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 3 - WAC - Posters 9 Bio-acidification of wines by Lachancea thermotolerans

Bio-acidification of wines by Lachancea thermotolerans

Abstract

Insufficient acidity in grapes from warm climates/vintages is commonly corrected through addition of tartaric acid during vinification, and less so with other organic acids. An alternative approach involves bio-acidification with the yeast Lachancea thermotolerans (LT) via lactic acid production during fermentation. Our work first elucidated the genetic (~200) and phenotypic (~100) diversity of LT strains, and then tested the performance of their subset in co-cultures with Saccharomyces cerevisiae (SC). In pure and mixed cultures alike, the modulation of acidity and other compositional parameters of wines depended on the LT strain, with either comparable or significant acidification relative to the SC control. An LT strain with exceptional bio-acidifying properties was selected, capable of lowering wine pH by ~0.5 units, and further characterised across a range of oenological conditions.

Our follow-up study aimed to i) compare the profiles of bio-acidified LT wines and acid-adjusted SC wines, and ii) evaluate the use of LT wines as blending components. For this purpose, high sugar/pH Merlot grapes were fermented with a sequential culture of LT and SC, and an SC monoculture. The aliquots of the SC control (pH 4) were acidified with either tartaric or lactic acid to the pH of the LT wine (pH 3.6), and the initial wines also blended in three proportions (1:3, 1:1, 3:1). Chemical analysis revealed major differences in a range of chemical parameters of wines (e.g. ethanol content, acidity parameters, volatile compounds, amino acids).  The compositional modulations were reflected in the sensory profiles of wines, as confirmed via ‘Rate-All-That-Apply’ evaluation by wine experts (n=30). Sensory profiles of the bio-acidified LT wine and the lactic acid-adjusted SC wine were similar, and contrasting to the tartaric acid-adjusted SC wine. Despite an identical initial matrix, lactic acid-adjusted SC wine had higher ‘red fruit’ flavour, and lower ‘hotness’, ‘bitterness’ and ‘body’ relative to tartaric acid-adjusted wine. This was driven by differences in ‘acidity’ perception, affected by titratable acidity (rather than pH) of wines. An inhibition of Brettanomyces bruxellensis growth was also observed in the bio-acidified LT wine and the lactic-acid adjusted SC wine. The profiles of blends were modulated depending on the proportion of the bio-acidified wine, thus highlighting the potential of this approach to boost ‘freshness’ and differentiate wine styles.

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Ana, Hranilovic, Marina Bely, Isabelle Masneuf-Pomarede, Joana Coulon, Warren Albertin, Vladimir Jiranek

Presenting author

Ana, Hranilovic – Department of Wine Science, The University of Adelaide, Australia

Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | BioLaffort, Floirac, France | Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | Department of Wine Science, The University of Adelaide, Australia,

Contact the author

Keywords

non-Saccharomyces yeasts – Lachancea thermotolerans – wine acidification – volatile composition – RATA sensory profiling

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Spatiotemporal patterns of chemical attributes in Vitis vinifera L. cv. Cabernet Sauvignon vineyards in Central California

Spatial variability of vine productivity in winegrapes is important to characterise as both yield and quality are relevant for the production of different wine styles and products. The objectives were to understand how patterns of variability of Cabernet Sauvignon fruit composition changed over time and space, how these patterns could be characterised with indirect measurements, and how spatial patterns of the variation in fruit compositional attributes can aid in improving management. Prior to the 2017 vintage, 125 data vines were distributed across each of four vineyards in the Lodi American Viticultural Area (AVA) of California. Each data vine was sampled at commercial harvest in 2017, 2018, and 2019. Yield components and fruit composition were measured at harvest for each data vine, and maps of yield and fruit composition were produced for eight ‘objective measures of fruit quality’: total anthocyanins, polymeric tannins, quercetin glycosides, malic acid, yeast assimilable nitrogen, β-damascenone, C6 alcohols and aldehydes, and 3-isobutyl-2-methoxypyrazine. Patterns of variation in anthocyanins and phenolic compounds were found to be most stable over time. Given this relative stability, management decisions focused on fruit quality could be based on zonal descriptions of anthocyanins or phenolics to increase profitability in some vineyards. In each vineyard, dormant season pruning weights and soil cores were collected at each location, elevation and soil apparent electrical conductivity surveys were completed, and remotely sensed imagery was captured by fixed wing aircraft and two satellite platforms at major phenological stages. The data collected were used to develop relationships among biophysical data, soil, imagery, and fruit composition. The standardised and aggregated samples from four vineyards over three seasons were included in the estimation of ‘common variograms’ to assess how this technique could aid growers in producing geostatistically rigorous maps of fruit composition variability without cumbersome, single season sampling efforts.

austrianvineyards.com: online viewer of all designations of Austrian wine

To digitally record and present all the origins of Austrian wines in the same perfect and clear way was the motivation for the Austrian Wine Marketing Board (Austrian Wine) to start with the project in 2018. In June 2021 the results were presented to the public in an online viewer showing all the designations of Austrian wine, available at https://austrianvineyards.com in a largely barrier-free manner. The online viewer provides tailored individual maps fitted to the respective zoom level. The smallest unit of wine-origins in Austria is called Ried and is displayed in a plot-specific manner highlighting areas under vine. Information on the Ried include administrative district, winegrowing municipality, cadastral municipality, large collective vineyard site, specific winegrowing region, generic winegrowing region, winegrowing area and, in many cases, an illustrative picture. Complementary data on the size, elevation (minimum-maximum), orientation (in 8 sectors plus flat) and gradient (minimum, maximum, average) are based on the area under vine according to the EU’s Integrated Administration and Control System. Additional information covers climate data. The diagrams are taken from the monthly breakdown of data in the annals of the Central Institute for Meteorology and Geodynamics, Austria provide a display of values for air temperature, precipitation, and sunshine hours for the reference year and the long-term average. Seasonal aggregated data on temperature, precipitation, and sunshine hours complete the display. Short descriptions with emphasis on geology and soil, field name in historical maps, etymology of the denomination, and main planted variety complements the available information for the main designations in the online viewer. These descriptions are compiled by winegrowers, geologists, historians, and journalists. All the information and data can be extracted to a pdf-file. Printed vineyard maps are also available. Missing content regarding wine origins in Styria will be completed in winter 2021/22.

Effect of fertigation strategies to adapt PGI Côtes de Gascogne production to hot vintage

The development of fertigation could be a possible solution to adapt PGI Côtes de Gascogne (south-western France) wine production to climate change. The goal would be to limit the negative effects of water stress on yield performance expectation (around 15 tons per hectare) and to make the use of fertilizers more efficient. This study aimed to compare the effects of three strategies of water and minerals supply on grapes and wines qualities. Two fertigation practices were compared to a rainfed control which is the current standard of the local grape growing production. The fertilizers (nitrogen and potassium) were (i) fully brought by irrigation pipe during the season, (ii) partially brought by irrigation pipe and partially on the soil or (iii) fully brought on the soil at the beginning of the season for the non-irrigated control (local standard). The trial was run on cv. Colombard trained on spur pruned with vertical shoot positioning system on a sandy-silty-clay soil over the 2020 vintage which was particularly hot for the region. Moderate to strong water deficit appeared during the growing period of the berries and held on after veraison. Irrigation strategies allowed for maintaining grapevine without water deficit and being significantly different from the control water status. Grapevine with fully or partial fertigation strategies produced 25% more yield mainly due to the increase of the bunch weight. Also, the fully fertigation showed the best ratio between yield and maturity and brought 30% less of fertilizers (both nitrogen and potassium) than the two other strategies. Finally, the analysis of aromatic compounds in Colombard wines, varietal thiols family, showed the same level of concentrations for the 3 treatments, confirming that the yield performance did not impact the aromatic potential in this trial.

Assessing the relationship between cordon strangulation, dieback, and fungal trunk disease symptom expression

Grapevine trunk diseases including Eutypa dieback are a major factor in the decline of vineyards and may lead to loss of productivity, reduced income, and premature reworking or replanting. Several studies have yielded results indicating that vines may be more likely to express symptoms of vascular disease if their health is already compromised by stress. In Australia and many other wine-growing regions it is a common practice for canes to be wrapped tightly around the cordon wire during the establishment of permanent cordon arms. It is likely that this practice may have a negative effect on health and longevity, as older cordons that have been trained in this manner often display signs of decay and dieback, with the wire often visibly embedded within the wood of the cordon. It is possible that adopting a training method which avoids constriction of the vasculature of the cordon may help to limit the onset of vascular disease symptom expression. A survey was conducted during the spring of two consecutive growing seasons on vineyards in South Australia displaying symptoms of Eutypa lata infection when symptomless shoots were 50–100 cm long. Vines were assessed as follows: (i) the proportion of cordon exhibiting dieback was rated using a 0–100% scale; (ii) the proportion of canopy exhibiting foliar symptoms of Eutypa dieback was rated using a 0–100% scale; (iii) the severity of strangulation was rated using a 0–4 point scale. Images were also taken of each vine for the purpose of measuring plant area index (PAI) using the VitiCanopy App. The goal of the survey was to determine if and to what extent any correlation exists between severity of strangulation and cordon dieback, in addition to Eutypa dieback foliar symptom expression.

Evaluation of climate change impacts at the Portuguese Dão terroir over the last decades: observed effects on bioclimatic indices and grapevine phenology

In the last decades the growers of the Portuguese Dão winegrowing region (center of Portugal) are experiencing changes in climate that are influencing either grape phenology berry health and ripening. Aiming to study the relationships between climate indices (CI), seasonal weather and grapevine phenology, in this work long-term climate and phenological data collected at the experimental vineyard of the Portuguese Dão research centre between 1958 and 2019 (61 years) for the red variety Touriga Nacional, was analyzed. The trends over time for the classical temperature-based indices (Growing Season Temperature – GST -, Growing Degree Days – GDD, Huglin Index – HI and Cool Night Index – CI) presented a significantly positive slope while the Dryness Index (DI) showed a negative trend over the last 61 years. Regarding grapevine phenology, an average advance of 4.5 days per decade in the harvest day was observed throughout the last 61 years. Consequently, the weather conditions during the ripening period have changed, showing an increasing trend over time in the average temperature (higher magnitude in the maximum than in the minimum temperature) and a decrease in the accumulated rainfall. A regression analysis showed that ~50% of harvest date variability over years was explained by the temperature-based indices variability. These observed effects of climate change on bioclimatic indices and corresponding anticipation of harvest date can still be considered advantageous for the Dão terroir as it allows to achieve an optimal berry ripening before the common equinox rains and, therefore, avoid the potential negative impacts of the rainfall on berry health and composition.