WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 3 - WAC - Posters 9 Bio-acidification of wines by Lachancea thermotolerans

Bio-acidification of wines by Lachancea thermotolerans

Abstract

Insufficient acidity in grapes from warm climates/vintages is commonly corrected through addition of tartaric acid during vinification, and less so with other organic acids. An alternative approach involves bio-acidification with the yeast Lachancea thermotolerans (LT) via lactic acid production during fermentation. Our work first elucidated the genetic (~200) and phenotypic (~100) diversity of LT strains, and then tested the performance of their subset in co-cultures with Saccharomyces cerevisiae (SC). In pure and mixed cultures alike, the modulation of acidity and other compositional parameters of wines depended on the LT strain, with either comparable or significant acidification relative to the SC control. An LT strain with exceptional bio-acidifying properties was selected, capable of lowering wine pH by ~0.5 units, and further characterised across a range of oenological conditions.

Our follow-up study aimed to i) compare the profiles of bio-acidified LT wines and acid-adjusted SC wines, and ii) evaluate the use of LT wines as blending components. For this purpose, high sugar/pH Merlot grapes were fermented with a sequential culture of LT and SC, and an SC monoculture. The aliquots of the SC control (pH 4) were acidified with either tartaric or lactic acid to the pH of the LT wine (pH 3.6), and the initial wines also blended in three proportions (1:3, 1:1, 3:1). Chemical analysis revealed major differences in a range of chemical parameters of wines (e.g. ethanol content, acidity parameters, volatile compounds, amino acids).  The compositional modulations were reflected in the sensory profiles of wines, as confirmed via ‘Rate-All-That-Apply’ evaluation by wine experts (n=30). Sensory profiles of the bio-acidified LT wine and the lactic acid-adjusted SC wine were similar, and contrasting to the tartaric acid-adjusted SC wine. Despite an identical initial matrix, lactic acid-adjusted SC wine had higher ‘red fruit’ flavour, and lower ‘hotness’, ‘bitterness’ and ‘body’ relative to tartaric acid-adjusted wine. This was driven by differences in ‘acidity’ perception, affected by titratable acidity (rather than pH) of wines. An inhibition of Brettanomyces bruxellensis growth was also observed in the bio-acidified LT wine and the lactic-acid adjusted SC wine. The profiles of blends were modulated depending on the proportion of the bio-acidified wine, thus highlighting the potential of this approach to boost ‘freshness’ and differentiate wine styles.

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Ana, Hranilovic, Marina Bely, Isabelle Masneuf-Pomarede, Joana Coulon, Warren Albertin, Vladimir Jiranek

Presenting author

Ana, Hranilovic – Department of Wine Science, The University of Adelaide, Australia

Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | BioLaffort, Floirac, France | Université of Bordeaux, UR œnologie, EA 4577, USC 1366 INRAE, Bordeaux INP, ISVV, Villenave d’Ornon, France | Department of Wine Science, The University of Adelaide, Australia,

Contact the author

Keywords

non-Saccharomyces yeasts – Lachancea thermotolerans – wine acidification – volatile composition – RATA sensory profiling

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.

Estimating bulk stomatal conductance of grapevine canopies

In response to changes in their environment, grapevines regulate transpiration using various physiological mechanisms that alter conductance of water through the soil-plant-atmosphere continuum. Expressed as bulk stomatal conductance at the canopy scale, it varies diurnally in response to changes in vapor pressure deficit and net radiation, and over the season to changes in soil water deficits and hydraulic conductivity of both soil and plant. It is necessary to characterize the response of conductance to these variables to better model how vine transpiration also responds to these variables. Furthermore, to be relevant for vineyard-scale modeling, conductance is best characterized using data collected in a vineyard setting. Applying a crop canopy energy flux model developed by Shuttleworth and Wallace, bulk stomatal conductance was estimated using measurements of individual vine sap flow, temperature and humidity within the vine canopy, and estimates of net radiation absorbed by the vine canopy. These measurements were taken on several vines in a non-irrigated vineyard in Bordeaux France, using equipment that did not interfere with ongoing vineyard operations. An inverted Penman-Monteith equation was then used to calculate bulk stomatal conductance on 15-minute intervals from July to mid-September 2020. Time-series plots show significant diurnal variation and seasonal decreases in conductance, with overall values similar to those in the literature. Global sensitivity analysis using non-parametric regression found transpiration flux and vapor pressure deficit to be the most important input variables to the calculation of bulk stomatal conductance, with absorbed net radiation and bulk boundary layer conductance being much less important. Conversely, bulk stomatal conductance was one of the most important inputs when calculating vine transpiration, further emphasizing the need for characterizing its response to environmental changes for use in vineyard water use modeling.

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

A predictive model of spatial Eca variability in the vineyard to support the monitoring of plant status

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Organic recycled mulches in sustainable viticulture: assessment of spontaneous plants communities and weed coverage

In recent years, developing more efficient and sustainable viticulture management has been essential due to the impact of climate change in semiarid regions. For this reason, the use of recycled organic mulching (ROM) in the vineyard has become an interesting strategy to cope with water stress, isolated soil from extreme temperatures and improving soil humidity, control the presence of weeds and therefore reduce the inputs of herbicides and improve soil fertility. This work aimed to analyse the effect of three different organic mulches [straw (S), grape pruning debris (GPD) and spent mushroom compost (SMC)] and two traditional soil management techniques [herbicide (H) and interrow (IN)] on weed coverage and the spontaneous plant communities’ presence. Data sampling was collected throughout the vine vegetative cycle of 2021 in La Rioja, Spain. The different soil management techniques had a clear effect on weed coverage and his development during the vine vegetative cycle. SMC and H were the treatments with the highest and the lowest coverage percentage, respectively. IN had a delayed weed emergence at the beginning of the vine vegetative cycle, but finally it reached maximum values nearby SMC. GPD and S had similar effects on weed emergence, reaching 25-30% of the maximum coverage values. A total of 29 herbaceous species were identified during the vegetative cycle, some of them very isolated and occasional. Principal component analysis (PCAs) showed a good association between spontaneous species and treatments, furthermore, specific species-treatment associations were found. Moreover, three clear groups of herbaceous communities were identified by cluster analysis. This study provides interesting information about the effect of different alternative soil management on herbaceous plant coverage and weed species communities which could contribute to making more sustainable viticulture.