WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 1 - WAC - Posters 9 Organic volatile compounds as suitable markers of grapevine response to defense elicitors in the vineyard

Organic volatile compounds as suitable markers of grapevine response to defense elicitors in the vineyard

Abstract

In greenhouse, emission of volatile organic compounds (VOC) by grapevine leaves has already been reported in response to the defence elicitor sulfated laminarin (PS3) [1]. In order to check that this response was not specific to PS3, experiments were conducted on Vitis cv Marselan plantlets with several other elicitors of different chemical structures: i.e. Bastid® (COS-OGA), chitosan, Redeli® (phosphonate), Romeo® (yeast extract) and Bion® (acibenzolar-S-methyl). Stir bar sorptive extraction (SBSE) was used as VOC sensor and volatiles compounds were analysed and identified by GC-MS. We confirmed that the observed increase in mono- and sesquiterpene emissions constitutes a common response of grapevine to elicitors in a time-dependent manner. Moreover, beta-ocimene and alpha-farnesene were systematically present within the emitted VOC “bouquet” [2]. Besides, stilbenes resveratrol and piceid were accumulated, but straight correlation with grapevine protection against downy mildew and those emissions terpenes and stilbenes could not be made. VOC emissions were then verified in two French vineyards in Burgundy and Bordeaux, respectively. VOC were analysed after Bastid® treatment of Vitis cvs Chardonnay and Cabernet franc at three phenological stages and using different collecting methods, i.e. passive or dynamic with either SBSE or Tenax sensors. As preliminary results, we observed that VOC emissions remain time-dependent and that terpenes, especially beta-ocimene, are also among the emitted volatiles. We found that the dynamic collect is more sensitive for VOC capture and is required in case of low level of emissions.
Overall these results suggest that VOC analysis could be a relevant method to further study vine response to defence elicitors in the vineyard.

References

[1] Chalal, M., J.B. Winkler, K. Gourrat, S. Trouvelot, M. Adrian, J.P. Schnitzler, F. Jamois and X. Daire, Sesquiterpene volatile organic compounds (VOCs) are markers of elicitation by sulfated laminarine in grapevine, Front Plant Sci, 6 (2015), 350.
[2] Lemaitre-Guillier, C., C. Dufresne, A. Chartier, S. Cluzet, J. Valls, L. Jacquens, A. Douillet, N. Aveline, M. Adrian and X. Daire, VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine, Molecules, 26(14) (2021).

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Christelle LEMAITRE-GUILLIER, Agnès CHARTIER, Christelle DUFRESNE, Antonin DOUILLET, Stéphanie CLUZET, Nicolas AVELINE, Xavier DAIRE, Marielle ADRIAN

Presenting author

Christelle LEMAITRE-GUILLIER – Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France

Institut de Chimie Organique et Analytique, ICOA, UMR 7311, Université d’Orléans, rue de Chartres, BP 6759, CEDEX 2, 45067 Orléans, France | Institut de Chimie Organique et Analytique, ICOA, UMR 7311, Université d’Orléans, rue de Chartres, BP 6759, CEDEX 2, 45067 Orléans, France | Institut Français de la Vigne et du Vin (IFV), 33290 Blanquefort, France | Equipe Molécules d’Intérêt Biologique, ISVV, Unité de Recherche Œnologie, EA 4577, USC 1366 INRAE, Faculté des Sciences Pharmaceutiques, Université de Bordeaux, CEDEX, 33882 Villenave d’Orno, France | Institut Français de la Vigne et du Vin (IFV), 33290 Blanquefort, France | Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France | Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France

Contact the author

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Soil quality in Beaujolais vineyard. Importance of pedology and cultural practices

A pedological study was carried out from 2009 to 2017 in Beaujolais vineyard, to improve physical and chemical knowledge of soils. It was completed in 2016 and 2017 by the current study, dealing with microbial aspects, in order to build a reference frame for improved advice in soil management. Microbial biomass was measured on representative plots of the six most common soil types identified in Beaujolais and, for each soil type, on plots with different levels of the main impacting parameters: total organic carbon, pH, cation exchange capacity, extractable copper. A total of 59 soil samples were collected. Confirming the results of various trials carried out in Beaujolais over the past 20 years, the results of the present study showed that the soils were still alive, but exhibited a large variability of biological parameters, which appeared dependant on both pedological and anthropic factors. Therefore, a good interpretation of biological parameters and advice for vine growers must rely on a pedologically-based referential with differentiated main driving factors. For example, the control of pH is of primary importance in granitic soils and in no way organic matter addition can improve soil quality if pH is too low. Conversely, in calcareous soils, biological parameters are more directly affected by direct or indirect (cover crops for example) inputs of organic matter. The use of biological parameters, such as microbial biomass, is of great potential value to improve advice on agro-viticultural practices (soil management, fertilization, liming, etc.), basis of a sustainable wine production on fragile soils.

Better understand the soil wet bulb formation with subsurface or aerial drip irrigation in viticulture

The gradual change in rainfall patterns experienced in the south of France vineyards, especially around the Mediterranean sea, means that the vines are increasingly subject to summer drought. The winegrowers developped the use of irrigation techniques to ensure the maintenance of competitive yields in the production of wines under Protected Geographical Indication label. In practice, drip irrigation pipes can be installed above the ground or buried into the soil as well as at different distances from the vine row. The objective of this study was to examine the profiles of the wet bulbs of the soil obtained from two drip irrigation systems : aerial drip located under the vine row and subsurface drip placed in the middle of the inter-row. This experiment took place over two consecutive seasons (2020-2021) on a 3.4 ha Viognier plot in the Mediterranean region (PGI Oc, France) on sandy clay soil. The annual rainfalls were less than 400 mm. Soil water content probes were installed at different depths (20 – 40 – 60 – 80 cm) and at different lateralities from the vine row (30 – 60 – 90 – 120 cm) to control the formation of the soil wet bulb during irrigation. The mapping and the analysis of the data allowed a better understanding and differentiation of the water percolation when irrigating with subsurface or aerial drip. For the same amount of water and without differences of vine water status, it is shown that in a subsurface drip irrigation situation, the size of the wet bulb formed is larger than in aerial drip irrigation system.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.

Mechanisms involved in the heating of the environment by the aerodynamic action of a wind machine to protect a vineyard against spring frost

One of the main consequences of global warming is the rise of the mean temperature. Thus, the heat summation by the plants begins sooner in the early spring, and by cumulating growing degree-days, phenological development tends to happen earlier. However, spring frost is still a recurrent phenomenon causing serious damages to buds and therefore, threatening the harvests of the winegrowers. The wind machine is a solution to protect fruit crops against spring frost that is increasingly used. It is composed of a 10-m mast with a blowing fan at its peak. By tapping into the strength of the nocturnal thermal inversion, it sweeps the crop by propelling warm air above to the ground. Thus, stratification is momentarily suppressed. Furthermore, the continuous action of the machine, alone or in synergy, or the addition of a heater allow the bud to be bathed in a warmer environment. Also, the punctual action of the tower’s warm gust reaches the bud directly at each rotation period. All these actions allow the bud to continuously warm up, but with different intensities and over a different period. Although there is evidence of the effectiveness of the wind machines, the thermal transfers involved in those mechanisms raise questions about their true nature. Field measurements based on ultrasonic anemometers and fast responding thermocouples complemented by laboratory measurements on a reduced scale model allow to characterize both the airflow produced by the wind machine and the local temperature in its vicinity. Those experiments were realized in the vineyard of Quincy, in the framework of the SICTAG project. In the future paper, we will detail the aeraulic characterization of the wind machine and the thermal effects resulting from it and we will focus on how the wind machine warms up the local atmosphere and enables to reduce the freezing risk.

Characterization of variety-specific changes in bulk stomatal conductance in response to changes in atmospheric demand and drought stress

In wine growing regions around the world, climate change has the potential to affect vine transpiration and overall vineyard water use due to related changes in atmospheric demand and soil water deficits. Grapevines control their transpiration in response to a changing environment by regulating conductance of water through the soil-plant-atmosphere continuum. Most vineyard water use models currently estimate vine transpiration by applying generic crop coefficients to estimates of reference evapotranspiration, but this does not account for changes in vine conductance associated with water stress, nor differences thought to exist between varieties. The response of bulk stomatal conductance to daily weather variability and seasonal drought stress was studied on Cabernet-Sauvignon, Merlot, Tempranillo, Ugni blanc, and Semillon vines in a non-irrigated vineyard in Bordeaux France. Whole vine sap flow, temperature and humidity in the vine canopy, and net radiation absorbed by the vine canopy were measured on 15-minute intervals from early July through mid-September 2020, together with periodic measurement of leaf area, canopy porosity, and predawn leaf water potential. From this data, bulk stomatal conductance was calculated on 15-minute intervals, and multiple regression analysis was performed to identify key variables and their relative effect on conductance. Attention was focused on addressing multicollinearity and time-dependency in the explanatory variables and developing regression models that were readily interpretable. Variability of vapor pressure deficit over the day, and predawn water potential over the season explained much of the variability in conductance, with relative differences in response coefficients observed across the five varieties. By characterizing this conductance response, the dynamics of vine transpiration can be better parameterized in vineyard water use modeling of current and future climate scenarios.