WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 1 - WAC - Posters 9 Organic volatile compounds as suitable markers of grapevine response to defense elicitors in the vineyard

Organic volatile compounds as suitable markers of grapevine response to defense elicitors in the vineyard

Abstract

In greenhouse, emission of volatile organic compounds (VOC) by grapevine leaves has already been reported in response to the defence elicitor sulfated laminarin (PS3) [1]. In order to check that this response was not specific to PS3, experiments were conducted on Vitis cv Marselan plantlets with several other elicitors of different chemical structures: i.e. Bastid® (COS-OGA), chitosan, Redeli® (phosphonate), Romeo® (yeast extract) and Bion® (acibenzolar-S-methyl). Stir bar sorptive extraction (SBSE) was used as VOC sensor and volatiles compounds were analysed and identified by GC-MS. We confirmed that the observed increase in mono- and sesquiterpene emissions constitutes a common response of grapevine to elicitors in a time-dependent manner. Moreover, beta-ocimene and alpha-farnesene were systematically present within the emitted VOC “bouquet” [2]. Besides, stilbenes resveratrol and piceid were accumulated, but straight correlation with grapevine protection against downy mildew and those emissions terpenes and stilbenes could not be made. VOC emissions were then verified in two French vineyards in Burgundy and Bordeaux, respectively. VOC were analysed after Bastid® treatment of Vitis cvs Chardonnay and Cabernet franc at three phenological stages and using different collecting methods, i.e. passive or dynamic with either SBSE or Tenax sensors. As preliminary results, we observed that VOC emissions remain time-dependent and that terpenes, especially beta-ocimene, are also among the emitted volatiles. We found that the dynamic collect is more sensitive for VOC capture and is required in case of low level of emissions.
Overall these results suggest that VOC analysis could be a relevant method to further study vine response to defence elicitors in the vineyard.

References

[1] Chalal, M., J.B. Winkler, K. Gourrat, S. Trouvelot, M. Adrian, J.P. Schnitzler, F. Jamois and X. Daire, Sesquiterpene volatile organic compounds (VOCs) are markers of elicitation by sulfated laminarine in grapevine, Front Plant Sci, 6 (2015), 350.
[2] Lemaitre-Guillier, C., C. Dufresne, A. Chartier, S. Cluzet, J. Valls, L. Jacquens, A. Douillet, N. Aveline, M. Adrian and X. Daire, VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine, Molecules, 26(14) (2021).

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Christelle LEMAITRE-GUILLIER, Agnès CHARTIER, Christelle DUFRESNE, Antonin DOUILLET, Stéphanie CLUZET, Nicolas AVELINE, Xavier DAIRE, Marielle ADRIAN

Presenting author

Christelle LEMAITRE-GUILLIER – Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France

Institut de Chimie Organique et Analytique, ICOA, UMR 7311, Université d’Orléans, rue de Chartres, BP 6759, CEDEX 2, 45067 Orléans, France | Institut de Chimie Organique et Analytique, ICOA, UMR 7311, Université d’Orléans, rue de Chartres, BP 6759, CEDEX 2, 45067 Orléans, France | Institut Français de la Vigne et du Vin (IFV), 33290 Blanquefort, France | Equipe Molécules d’Intérêt Biologique, ISVV, Unité de Recherche Œnologie, EA 4577, USC 1366 INRAE, Faculté des Sciences Pharmaceutiques, Université de Bordeaux, CEDEX, 33882 Villenave d’Orno, France | Institut Français de la Vigne et du Vin (IFV), 33290 Blanquefort, France | Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France | Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France

Contact the author

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

Differential responses of red and white grape cultivars trained to a single trellis system – the VSP

Commercial grape production relies on training grapevine cultivars onto a variety of trellis systems. Training allows for well-lit leaves and clusters, maximizing fruit quality in addition to facilitating cultivation, harvesting, and diseases control. Although grapevines can be trained onto an infinite variety of trellis systems, most red and white cultivars are trained to the standard VSP (Vertical Shoot Positioning) system. However, red and white cultivars respond differently to VSP in fruit composition and growth characteristics, which are yet to be fully understood. Therefore, the objective of this study was to examine the influence of the VSP trellis system on fruit composition of three red, Cabernet Sauvignon, Merlot and Syrah, and three white, Chardonnay, Riesling, and Gewurztraminer cultivars grown under uniform growing conditions in the same vineyard. All cultivars were monitored for maturity and harvested at their physiologically maximum possible sugar concentration to compare various fruit quality attributes such as Brix, pH, TA, malic and tartaric acids, glucose and fructose, potassium, YAN, and phenolic compounds including total anthocyanins, anthocyanin profile, and tannins. A distinct pattern in fruit composition was observed in each cultivar. In regards to growth characteristics, Syrah grew vigorously with the highest cluster weight. Although all cultivars developed pyriform seeds, the seed size and weight varied among all cultivars. Also varied were mesocarp cell viability, brush morphology, and cane structure. This knowledge of the canopy architectural characteristics assessed by the widely employed fruit compositional attributes and growth characteristics will aid the growers in better management of the vines in varied situations.

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

Is wine terroir a valid concept under a changing climate?

The OIV[i] defines terroir as a concept referring to an area in which collective knowledge of the interactions between the physical and biological environment (soil, topography, climate, landscape characteristics and biodiversity features) and vitivinicultural practices develops, providing distinctive wine characteristics. Those are perceptible in the taste of wine, which drives consumer preference and, therefore, wine’s value in the marketplace. Geographical indications (GI) are recognized regulatory constructs formalizing and protecting the nexus between wine taste and the terroir generating it. Despite considering updates, GIs do not consider the nexus as a dynamic one and do not anticipate change, namely of climate. Being climate a fundamental feature of terroir, it strongly impacts wine characteristics, such as taste. According to IPCC[ii], many widespread, rapid and unprecedented changes of climate occurred, some being irreversible over hundreds to thousands of years. Climatic shifts and atmospheric-driven extreme events have been widely reported worldwide. Recent climatic trends are projected to strengthen in upcoming decades, whereas extremes are expected to increase in frequency and intensity, forcing wines away from GI definitions. Geographical shifts of viticultural suitability are projected, often moving into regions and countries different from current ones. Some authors propose adaptation in viticulture, winemaking and product innovation. We show evidence of climate changing wine characteristics in the Douro valley, home of 270-year-old Port GI. We discuss herein resist or adapt stances for when climate changes the nexus between terroir and wine characteristics. Using the MED-GOLD[iii] dashboard, a tool allowing for easy visual navigation of past and future climates, we demonstrate how policymakers can identify future moments, throughout the 21st century under different emission scenarios, when GI specifications will likely need updates (e.g., boundaries, varieties) to reduce climate-change impacts.

Climate modeling at local scale in the Waipara winegrowing region in the climate change context

In viticulture, a warming climate can have a very significant impact on grapevine development and therefore on the quality and characteristics of wines across different spatial scales, ranging from global to local. In order to adapt wine-growing to climate change, global climate models can be used to define future scenarios, but only at the scale of major wine regions. Despite the huge progress made over the last ten years in terms of the spatial resolution of climate models (now downscaled to a few square kilometres), they are not yet sufficiently precise to account for the local climate variability associated with such parameters as local topography, in spite of these parameters being decisive for vine and wine characteristics. This study describes a method to downscale future climate scenarios to vineyard scale. Networks of data loggers have been used to collect air temperature at canopy level in the Waipara winegrowing region (New Zealand) over five growing seasons. These measurements allow the creation of fine-scale geostatistical models and maps of temperature (at 100 m resolution) for the growing season. In order to model climate change at pilot site scale, these geostatistical models have been combined with regional climate change predictions for the periods 2031-2050 and 2081-2100 based on the RCP8.5 climate change scenario. The integration of local climate variability with regionalized climate change simulations allows assessment of the impacts of climate change at the vineyard scale. The improved knowledge gained using this methodology results from the increased horizontal resolution that better addresses the concerns of winegrowers. The results provide the local winegrowers with information necessary to understand current processes, as well as historical and future viticulture trends at the scale of their site, thereby facilitating decisions about future response strategies.