WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 1 - WAC - Posters 9 Organic volatile compounds as suitable markers of grapevine response to defense elicitors in the vineyard

Organic volatile compounds as suitable markers of grapevine response to defense elicitors in the vineyard

Abstract

In greenhouse, emission of volatile organic compounds (VOC) by grapevine leaves has already been reported in response to the defence elicitor sulfated laminarin (PS3) [1]. In order to check that this response was not specific to PS3, experiments were conducted on Vitis cv Marselan plantlets with several other elicitors of different chemical structures: i.e. Bastid® (COS-OGA), chitosan, Redeli® (phosphonate), Romeo® (yeast extract) and Bion® (acibenzolar-S-methyl). Stir bar sorptive extraction (SBSE) was used as VOC sensor and volatiles compounds were analysed and identified by GC-MS. We confirmed that the observed increase in mono- and sesquiterpene emissions constitutes a common response of grapevine to elicitors in a time-dependent manner. Moreover, beta-ocimene and alpha-farnesene were systematically present within the emitted VOC “bouquet” [2]. Besides, stilbenes resveratrol and piceid were accumulated, but straight correlation with grapevine protection against downy mildew and those emissions terpenes and stilbenes could not be made. VOC emissions were then verified in two French vineyards in Burgundy and Bordeaux, respectively. VOC were analysed after Bastid® treatment of Vitis cvs Chardonnay and Cabernet franc at three phenological stages and using different collecting methods, i.e. passive or dynamic with either SBSE or Tenax sensors. As preliminary results, we observed that VOC emissions remain time-dependent and that terpenes, especially beta-ocimene, are also among the emitted volatiles. We found that the dynamic collect is more sensitive for VOC capture and is required in case of low level of emissions.
Overall these results suggest that VOC analysis could be a relevant method to further study vine response to defence elicitors in the vineyard.

References

[1] Chalal, M., J.B. Winkler, K. Gourrat, S. Trouvelot, M. Adrian, J.P. Schnitzler, F. Jamois and X. Daire, Sesquiterpene volatile organic compounds (VOCs) are markers of elicitation by sulfated laminarine in grapevine, Front Plant Sci, 6 (2015), 350.
[2] Lemaitre-Guillier, C., C. Dufresne, A. Chartier, S. Cluzet, J. Valls, L. Jacquens, A. Douillet, N. Aveline, M. Adrian and X. Daire, VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine, Molecules, 26(14) (2021).

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Christelle LEMAITRE-GUILLIER, Agnès CHARTIER, Christelle DUFRESNE, Antonin DOUILLET, Stéphanie CLUZET, Nicolas AVELINE, Xavier DAIRE, Marielle ADRIAN

Presenting author

Christelle LEMAITRE-GUILLIER – Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France

Institut de Chimie Organique et Analytique, ICOA, UMR 7311, Université d’Orléans, rue de Chartres, BP 6759, CEDEX 2, 45067 Orléans, France | Institut de Chimie Organique et Analytique, ICOA, UMR 7311, Université d’Orléans, rue de Chartres, BP 6759, CEDEX 2, 45067 Orléans, France | Institut Français de la Vigne et du Vin (IFV), 33290 Blanquefort, France | Equipe Molécules d’Intérêt Biologique, ISVV, Unité de Recherche Œnologie, EA 4577, USC 1366 INRAE, Faculté des Sciences Pharmaceutiques, Université de Bordeaux, CEDEX, 33882 Villenave d’Orno, France | Institut Français de la Vigne et du Vin (IFV), 33290 Blanquefort, France | Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France | Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France

Contact the author

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Effects of graft quality on growth and grapevine-water relations

Climate change is challenging viticulture worldwide compromising its sustainability due to warmer temperatures and the increased frequency of extreme events. Grafting Vitis vinifera L.

Sustainable fertilisation of the vineyard in Galicia (Spain)

Excessive fertilization of the vineyard leads to low quality grapes, increased costs and a negative impact on the environment. In order to establish an integrated management system aimed at a sustainable fertilization of the vineyards, nutritional reference levels were established. For this purpose, 30 representative vineyards of the Albariño variety were studied, in which soil and petiole analyses were carried out for two years and grape yield and quality at harvest were measured. In both years of study, soil pH, calcium, sodium and cation exchange capacity were positively correlated with calcium content and negatively correlated with manganese in grapes. Irrigated vineyards had higher levels of aluminium in soil and lower levels of calcium in petiole. Climatic conditions were very different in the years of the study. The year 2019 was colder than usual, in 2020 there was a marked water stress with high summer temperatures. This resulted in medium-high acidity in grapes in 2019 and low acidity in 2020, with sugar levels being similar both years. A very marked decrease in must amino nitrogen was observed in 2020, with ammonia nitrogen remaining stable. The correlation of acidity and sugar values in grapes with soil and petiole analysis data made it possible to establish reference levels for the nutritional diagnosis of the Albariño variety in this region. Based on these results, an easy-to-use TIC application is currently being created for grapegrowers, aimed at improving the sustainability of the vineyard through reasoned fertilization. This study has now been extended to other Galician vine varieties.

The effects of alternative herbicide free cover cropping systems on soil health, vine performance, berry quality and vineyard biodiversity in a climate change scenario in Switzerland

There is an urgent need in viticulture to adopt alternative herbicide-free soil management strategies to mitigate climate change, increase biodiversity, reduce plant protection products and improve soil quality while minimizing detrimental effects on grapevine’s stress tolerance and fruit quality. To propose sustainable solutions, adapted to different pedoclimatic conditions in Switzerland, we developed a multidisciplinary 4-year project, started in 2020. Objectives of the project are to a) evaluate the impact of green covers (spontaneous flora, winter cover crop and permanent ground cover) on environmental and agronomic parameters and b) develop subsequently innovative strategies for different viticultural contexts of Switzerland. The project is divided into 3 phases: 1) diagnosis, 2) on-farm and 3) on-station experiments. Phase 1) consisted in an assessment of 30 commercial vineyards all over Switzerland, where growers already use different herbicide-free soil management strategies. The most promising practices identified in this exploratory phase will be replicated in commercial vineyards across Switzerland (“on-farm”) as well as in a classical randomized block design in an experimental plot (“on-station”). For phase 1), measurements consisted in evaluation of soil status (compaction, structure, roots development), soil microbial diversity (metagenomics), plant diversity and biomass, vine physiology (water stress, vigor, leaf nitrogen) and berry quality (acidity, sugar, available nitrogen). Interestingly, the permanent ground cover resulted in a higher Shannon index thus a higher biodiversity as compared to the other itineraries. The winter cover crop increased vine nitrogen and vigor while deteriorating soil quality, leaving the soil more exposed and compacted likely due to more frequent tillage. The spontaneous flora led to higher berry sugar accumulation, less nitrogen and higher malic acid concentration putatively due to a higher water retention of the flora in a particularly wet vintage. Phases 2) and 3) are required to confirm those tendencies, over the 3 next vintages and different climatic conditions.

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Grape must quality and mesoclimatic variability in Fruška Gora wine-growing region, Serbia

The Fruška Gora mountain is a traditional wine-growing region in Serbia situated in the Pannonian Basin. Due to such a position, the vicinity of the Danube River and the presence of concave configuration, it is suitable for grape production. This paper provides analyses of spatial variations in meteorological parameters and grape juice quality within Fruška Gora wine region over three consecutive vintages (2018-2020). The examined period can be defined as warm with cool nights during September (AVG 18,9°C; GDD 1918°C; CI 12°CF) and with the presence of mesoclimatic variability. The East part of the study area was somewhat drier and hotter compared to other parts of the region. The analyses of grape must samples (190 in total) of five cultivars (Cabernet-Sauvignon, Merlot, Chardonnay, Sauvignon blanc and Grašac (Welschriesling)) commonly grown across the region (19 sites), were performed using Fourier Transform Infrared Technology (FTIR). Among all cultivars, Sauvignon blanc was harvested first in the East area (DOY=246±5, GDD at harvest=1552±74, 22.2±0.7 °Brix), while the latest harvest was recorded for Cabernet-Sauvignon in the West (DOY=283±5, GDD at harvest=1936±187, 23.4±1.0 °Brix ). Both the red and white cultivars had higher acidity and YAN in the grape must if the vines were grown in the North and East compared to South and West areas. According to PCA analysis, Grašac showed the lowest variation in grape must chemical composition. Thus, the results confirm that Grašac is the most stable cultivar in Fruška Gora. All monitored cultivars reached technological fruit ripeness by the end of the growing season. However, it was difficult to reach full ripeness of red cultivars, mostly beacuse of uncoupling of technolocical and phenolic ripeness. Thus, Cabernet-Sauvignon had higher variations in GDD sums at harvest compared to other cultivars, which probably increased variations in grape must quality.