WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 1 - WAC - Posters 9 Organic volatile compounds as suitable markers of grapevine response to defense elicitors in the vineyard

Organic volatile compounds as suitable markers of grapevine response to defense elicitors in the vineyard

Abstract

In greenhouse, emission of volatile organic compounds (VOC) by grapevine leaves has already been reported in response to the defence elicitor sulfated laminarin (PS3) [1]. In order to check that this response was not specific to PS3, experiments were conducted on Vitis cv Marselan plantlets with several other elicitors of different chemical structures: i.e. Bastid® (COS-OGA), chitosan, Redeli® (phosphonate), Romeo® (yeast extract) and Bion® (acibenzolar-S-methyl). Stir bar sorptive extraction (SBSE) was used as VOC sensor and volatiles compounds were analysed and identified by GC-MS. We confirmed that the observed increase in mono- and sesquiterpene emissions constitutes a common response of grapevine to elicitors in a time-dependent manner. Moreover, beta-ocimene and alpha-farnesene were systematically present within the emitted VOC “bouquet” [2]. Besides, stilbenes resveratrol and piceid were accumulated, but straight correlation with grapevine protection against downy mildew and those emissions terpenes and stilbenes could not be made. VOC emissions were then verified in two French vineyards in Burgundy and Bordeaux, respectively. VOC were analysed after Bastid® treatment of Vitis cvs Chardonnay and Cabernet franc at three phenological stages and using different collecting methods, i.e. passive or dynamic with either SBSE or Tenax sensors. As preliminary results, we observed that VOC emissions remain time-dependent and that terpenes, especially beta-ocimene, are also among the emitted volatiles. We found that the dynamic collect is more sensitive for VOC capture and is required in case of low level of emissions.
Overall these results suggest that VOC analysis could be a relevant method to further study vine response to defence elicitors in the vineyard.

References

[1] Chalal, M., J.B. Winkler, K. Gourrat, S. Trouvelot, M. Adrian, J.P. Schnitzler, F. Jamois and X. Daire, Sesquiterpene volatile organic compounds (VOCs) are markers of elicitation by sulfated laminarine in grapevine, Front Plant Sci, 6 (2015), 350.
[2] Lemaitre-Guillier, C., C. Dufresne, A. Chartier, S. Cluzet, J. Valls, L. Jacquens, A. Douillet, N. Aveline, M. Adrian and X. Daire, VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine, Molecules, 26(14) (2021).

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Christelle LEMAITRE-GUILLIER, Agnès CHARTIER, Christelle DUFRESNE, Antonin DOUILLET, Stéphanie CLUZET, Nicolas AVELINE, Xavier DAIRE, Marielle ADRIAN

Presenting author

Christelle LEMAITRE-GUILLIER – Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France

Institut de Chimie Organique et Analytique, ICOA, UMR 7311, Université d’Orléans, rue de Chartres, BP 6759, CEDEX 2, 45067 Orléans, France | Institut de Chimie Organique et Analytique, ICOA, UMR 7311, Université d’Orléans, rue de Chartres, BP 6759, CEDEX 2, 45067 Orléans, France | Institut Français de la Vigne et du Vin (IFV), 33290 Blanquefort, France | Equipe Molécules d’Intérêt Biologique, ISVV, Unité de Recherche Œnologie, EA 4577, USC 1366 INRAE, Faculté des Sciences Pharmaceutiques, Université de Bordeaux, CEDEX, 33882 Villenave d’Orno, France | Institut Français de la Vigne et du Vin (IFV), 33290 Blanquefort, France | Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France | Agroécologie, Institut Agro Dijon, CNRS, INRAe, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France

Contact the author

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Late season canopy management practices to reduce sugar loading and improve color profile of Cabernet-Sauvignon grapes and wines in the high irradiance and hot conditions of California Central Valley

Global warming is accelerating grape ripening, leading to unbalanced wines from fruit with high sugar content but poor aroma and colour development. Reducing the size of the photosynthetic apparatus after veraison has been shown to delay technological ripeness in cool climates, but methods have not been tested in areas with high irradiance and temperature where fruit exposure could have disastrous effects on berry composition. In this Cabernet-Sauvignon trial, we compared the application of an antitranspirant (pinolene), to severe canopy topping and above bunch zone leaf removal, all performed at mid-ripening, with an untouched control. We monitored the vines weekly by measuring stem water potential, gas exchange, fruit zone light exposure. We sampled berries to measure berry weight, total soluble solids, pH, titratable acidity, and the anthocyanin profile. At harvest, we assessed yield components, measured carbon isotope discrimination, rated sunburn on clusters, and produced experimental wines. We submitted harvest samples to metabolomic profiling through PFP-Q Exactive MS/MS and wines to sensory analysis. Application of the antitranspirant significantly reduced stomatal conductance and assimilation rate but did not affect the stem water potential. Inversely, leaf removal and topping increased water potential but did not affect leaf gas exchange. The late topping was the only treatment able to decrease sugar content (up to 2Bx), increase titratable acidity and pH, and improve anthocyanin content because of lower degradation of di-hydroxylated forms. Late leaf removal above the bunch zone increased lightning conditions in the canopy and produced the most significant damage on fruits. Yield components were not affected. This work suggests that late-season canopy management can effectively control ripening speeds and improve grapes and wines. Still, the effect on grape exposure in a critical time must be well balanced to avoid problems with the appropriate technique.

Under-vine management effects on grapevine production, soil properties and plant communities in South Australia

Under-vine (UV) management has traditionally consisted of synthetic herbicide use to limit competition between weeds and grapevines. With growing global interest towards non-synthetic chemical use, this study aimed to capture the effects of alternative UV management at two commercial Shiraz vineyards in South Australia, where the sole management variables were UV management since 2016. In adjacent treatment blocks, cultivation (CU) was compared to spontaneous vegetation (SV) in McLaren Vale (MV), and herbicide was compared to SV in Eden Valley (EV). Soil water infiltration rates were slower and grapevine stem water potential was lower in CU compared to SV in MV, with the latter having a plant community dominated by soursob (Oxalis pes-caprae) during winter; while in EV, there was little separation between the treatments. Yields were affected at both sites, with SV being higher in MV and HE being higher in EV. In MV, the only effect on grape must was a lower 13C:12C isotope ratio in CU, indicating greater grapevine water stress. In the grape must at EV, SV had higher total soluble solids, total phenolics, anthocyanins, and yeast available nitrogen; and lower pH and titratable acidity. Pruning weights were not affected by the treatments in MV, while they were higher in HE at EV. Assessments revealed that the differing soil types at the two sites were likely the main determinants of the opposing production outcomes associated with UV management. In the silty loam soil of MV, the higher yields in SV were likely due to more plant-available water, as a potential result of the continuous soil bio-pores formed by winter UV vegetation. Conversely, in the loamy sand soils of EV with a lower cation exchange capacity, the lower yields and pruning weights in SV suggest the UV vegetation competed significantly with the grapevines for available water and nutrients.

Modeling the suitability of Pinot Noir in Oregon’s Willamette Valley in a changing climate

Air temperature is the key driver of grapevine phenology and a significant environmental factor impacting yield and quality for a winegrape growing region. In this study the optimal downscaled CMIP5 ensemble for computing thegrowing season average temperature (GST) viticulture climate classification index was determined to spatially compute on a decadal basis predictions of the GST climate index and the grapevine sugar ripeness (GSR) model for Pinot Noir throughout the Willamette Valley (WV) American Viticultural Area (AVA). Forecasts for average temperature and a 220 g/L target sugar concentration level were computed using daily Localized Constructed Analogs (LOCA) downscaled CMIP5 historic and Representative Concentration Pathways (RCP) future climate projections of minimum and maximum daily temperature. We explore spatiotemporal trends of the GST climate classification index and Pinot Noir specific applications of the GSR phenology model for the WV AVA. Spatiotemporal computations of the GST climate index and Pinot Noir specific applications of the GSR model enable the opportunity to explore relationships between their computed values with one intent being to provide updated GST ranges that better align with current temperature-based modeling understanding of Pinot Noir grapevine phenology and the viticultural application of LOCA CMIP5 climate projections for the WV AVA. The Pinot Noir specific applications of the GSR model or the GST index with updated bounds indicate that the percent of the WV AVA area suitable for Pinot Noir production is currently at or near its peak value in the upper 80s to lower 90s of this century.

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,