GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Assessing macro-elements contents in vine leaves and grape berries of Vitis vinifera using near-infrared spectroscopy coupled with chemometrics

Assessing macro-elements contents in vine leaves and grape berries of Vitis vinifera using near-infrared spectroscopy coupled with chemometrics

Abstract

Context and purpose of the study – The cultivated vine (Vitis vinifera) is the main species cultivated in the world to make wine. In 2017, the world wine market represents 29 billion euros in exports, and France contributes 8.2 billion (28%) to this trade, making it a traditional market of strategic importance. Viticulture is therefore a key sector of the French agricultural economy. It is in this context that the nutritional diagnosis of the vine is of real strategic interest to winegrowers. Indeed, the fertilization of the vine is a tool for the winegrower that allows him to influence and regulate the quality of the wine. Nowadays, nutrition analysis is made with CHNS analyzer for elemental particles, and mass-spectroscopy for macro and microelements. Such methods are destructive and time consuming, then results could be obsolete for the vine grower. Near-infrared spectroscopy coupled with chemometrics tools allows to developed models of prediction that can provide accurate information about nutrition status of the vine in the field. In this study, we concentrate on the relative amount of Carbon [C], Hydrogen [H], Nitrogen [N], Sulphur [S] in dry matter (DM) and the C:N ratio.

Material and methods – 252 samples of different organs (leaves blade, leaves petioles, pea sized berries and berries at véraison) of 4 varieties (Muscat, Chasselas, Négrette and Sauvignon blanc) were analyzed. Spectrum were taken on both fresh material and dried ones with a reflectance spectrometer. The spectra were pre-processed using multiple scatter correction (MSC) and 1st and 2nd order Savitsky-Golay derivative (D1 and D2), before developing the cross-validation models using partial least square (PLS) regression and test it on a prediction set.

Results – The coefficient of determination in prediction (r²), the roots mean square error of prediction (RMSEP) and the ratio of performance of prediction (RPD) were obtained for C (0.49, 14.6% of DM and 1.33 on fresh material with MSC, 0.45, 15.4% of DM and 1.26 on dry material with MSC), H (0.56, 1.71% of DM and 1.45 on fresh material with D1, 0.49, 1.88% of DM and 1.32 on dry material with D1), N (0.91, 1.12% of DM, 3.32 on fresh material with raw spectra, 0.95, 0.84% of DM and 4.39 on dry material with MSC), S (0.47, 0.319% of DM and 1.31 on fresh material with MSC, 0.46, 0.322% of DM and 1.30 on dry material with D2) and C:N ratio (0.85, 8.20 and 2.58 on fresh material with raw spectra, 0.87, 7.55 and 2.80 on dry material with D2). Results show that the near-infrared reflectance spectroscopy can be used to assessing the level of nitrogen nutrition in vine and the C:N ratio. All model performance could be improved by increasing the number of samples.

DOI:

Publication date: March 11, 2024

Issue: GiESCO 2019

Type: Poster

Authors

Sebastien CUQ1*, Valerie LEMETTER2, Olivier GEFFROY1, Didier KLEIBER1, Cecile LEVASSEUR-GARCIA3

1 Physiologie, Pathologie et Génétique Végétales (PPGV), Université de Toulouse, INP-PURPAN, Toulouse, France
2 Plateforme TOAsT, Université de Toulouse, INP-PURPAN, Toulouse, France
3 Laboratoire de Chimie Agro-industrielle (LCA), Université de Toulouse, INRA, INPT, INP-PURPAN, Toulouse, France

Contact the author

Keywords

Infrared, Spectroscopy, Elemental analysis, Vitis vinifera

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Effect of vigour and number of clusters on eonological parameters and metabolic profile of Cabernet Sauvignon red wines

Vegetative growth and yield are reported to affect grape and wine quality. They can be controlled through different techniques linked to vine management. The objective of this research was to determine the effect of vine vigour and number of clusters per vine on physicochemical composition and phenolic profile of red wines. The experiment was carried out during two vegetative cycles, with cv. Cabernet Sauvignon grafted onto Paulsen 1103. Three vine vigour were defined, according to shoot weight at previous harvests, being low, medium and high. Five treatments of number of clusters were used for each vigour, with 15, 22, 29, 36, and 45 clusters per vine. Grapes from all treatments were harvested in the same day from Brix and total acidity criteria. Thirty days after bottling, classical analyzes and phenolic compounds were performed. As results, different responses were obtained from each vintage. In 2020, a dry season from veraison to harvest, grapes and wines obtained from low vigour treatment and 45 clusters per vine was the highest in sugar and alcohol content respectively, while grapes and wines from high vigour and 15 clusters presented the lowest sugar and alcohol content. Total anthocyanins were higher in treatment with low vigour and 15 clusters, while the lowest amounts were found in low vigour with 45 clusters, as well as medium and high vigour with 36 clusters per vine. Total tannins were higher in high vigour with 22 clusters and medium vigour with 29 clusters, while were lower in low vigour with 36 clusters. In 2021, a wet season at harvest, responses were different, and great variations were observed between treatments. As conclusions, yield and vine vigour had strong influence on grape and wine quality, promoting different enological potentials on which can be indicated/used for aging strategies of red and even rosé wines.

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

Simulating climate change impact on viticultural systems in historical and emergent vineyards

Global climate change affects regional climates and hold implications for wine growing regions worldwide. Although winegrowers are constantly adapting to internal and external factors, it seems relevant to develop tools, which will allow them to better define actual and future agro-climatic potentials. Within this context, we develop a modelling approach, able to simulate the impact of environmental conditions and constraints on vine behaviour and to highlight potential adaptation strategies according to different climate change scenarios. Our modeling approach, named SEVE (Simulating Environmental impacts on Viticultural Ecosystems), provides a generic modeling framework for simulating grapevine growth and berry ripening under different conditions and constraints (slope, aspect, soil type, climate variability…) as well as production strategies and adaptation rules according to climate change scenarios. Each activity is represented by an autonomous agent able to react and adapt its reaction to the variability of environmental constraints. Using this model, we have recently analyzed the evolution of vineyards’ exposure to climatic risks (frost, pathogen risk, heat wave) and the adaptation strategies potentially implemented by the winegrowers. This approach, implemented for two climate change scenarios, has been initiated in France on traditional (Loire Valley) and emerging (Brittany) vineyards. The objective is to identify the time horizons of adaptations and new opportunities in these two regions. Carried out in collaboration with wine growers, this approach aims to better understand the variability of climate change impacts at local scale in the medium and long term.

Copper contamination in vineyard soils of Bordeaux: spatial risk assessment for the replanting of vines and crops

Copper (Cu) is widely and historically used in viticulture as a fungicide against mildew. Cu has a strong affinity for soil organic matter and accumulates in topsoil horizons. Thus, Cu may negatively affect soil organisms and plants, consequently reducing soil fertility and productivity. The Bordeaux vineyards have the largest vineyard surfaces (26%) within French controlled appellation and a great proportion of French wine production (around 5 million hl per year). Considering the local context of vineyard surfaces decreasing (vine uprooting) and possible new crop plantation, the issue of Cu potential toxicity rises. Therefore, the aims of this work are firstly to evaluate the Cu contamination in vineyard soils of Bordeaux, secondly to produce a risk assessment map for new vine or crop plantation. We used soil analyses from several local studies to build a database with 4496 soil horizon samples. The database was enhanced by means of pedotransfer functions in order to estimate the bioaccessible (EDTA-extractable) Cu in soils of samples without measurements. From this database, 1797 georeferenced samples with CuEDTA concentrations in the topsoil (0-50 cm depth) were used for kriging interpolation in order to produce the spatial distribution map of CuEDTA in vineyard soils. Then, the spatial distribution of Cu was crossed with vine uprooting surfaces and municipality boundaries. CuEDTAconcentrations ranged from 0.52 to 459 mg/kg and showed clear anomalies. Our results from spatial analysis showed that almost 50% of vineyard soil surfaces have CuEDTA concentrations higher than 30 mg/kg (moderate risk for new plantation) and 20% with concentrations higher than 50 mg/kg (high risk for new plantation). A decision-support map based on municipalities was realised to provide a simple tool to stakeholders concerned by land use management.

Towards a regional mapping of vine water status based on crowdsourcing observations

Monitoring vine water status is a major challenge for vineyard management because it influences both yield and harvest quality. It is also a challenge at the territorial scale for identifying periods of high water restriction or zones regularly impacted by water stress. This information is of major importance for defining collective strategies, anticipating harvest logistic or applying for irrigation authorisation. At this spatial scale, existing tools and methods for monitoring vine water status are few and often require strong assumptions (e.g. water balance model). This paper proposes to consider a collaborative collection of observations by winegrowers and wine industry stakeholders (crowdsourcing) as an interesting alternative. Indeed, it allows the collection of a large number of field observations while pooling the collection effort. However, the feasibility of such a project and its interest in monitoring vine water status at regional scale has never been tested.

The objective of this article is to explore the possibility of making a regional map of vine water status based on crowdsourcing observations. It is based on the study of the free mobile application ApeX-Vigne, which allows the collection of observations about vine shoot growth. This information is easy to collect and can be considered, under certain conditions, as a proxy for vine water status. This article presents the first results obtained from the nearly 18,000 observations collected by winegrowers and wine industry stakeholders during 2019, 2020 and 2021 seasons. It presents the vine shoot growth maps obtained at regional scale and their evolution over the three vintages studied. It also proposes an analysis of the factors that favoured the number of observations collected and those that favoured their quality. These results open up new perspectives for monitoring vine water status at a regional scale but above they provide references for other crowdsourcing projects in viticulture.