GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Volatile organic compounds investigation in Müller Thurgau wines obtained from vineyard treated with biochar

Volatile organic compounds investigation in Müller Thurgau wines obtained from vineyard treated with biochar

Abstract

Context and purpose of the study – Volatile Organic Compounds (VOCs) are responsible for the flavor and aroma of a wine. The sensory qualities of the wines depend not only on grape intrinsic characteristics, but also on extrinsic factors including the soil composition. Previous studies have shown that the application of pyrogenic carbon (biochar) can lead to a change in soil parameters. For that reason, one of the goals of the ERDF funded project «WoodUp» is the characterization and reutilization of the locally produced biochar for agricultural purposes. In this study wine quality is investigated to better understand how the chemical and physical modification of the soil can influence the wine VOCs profile from Müller-Thurgau, after biochar application.

Material and methods – Wines obtained from vineyard treated with different amounts of biochar were analyzed (3.9 kg/ m² dry matter compost, 2.5 kg/m² dry matter biochar, 5 kg/m² dry matter biochar, 2.5 kg/m² dry matter biochar plus 3.9 kg/ m² dry matter compost, 5 kg/m² dry matter biochar plus 3.9 kg/ m² dry matter compost and the untreated as control). Samples, 1.5 ml of each wine, were placed into 20 ml glass vial with the addition of 0.45 g of NaCl and 5 μl of 2-octanol (123 ppm) as internal standard. The volatile composition of wines was determined by using headspace solid phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC–MS) in full scan mode. The headspace was sampled using a DVB/CAR/PDMS 50/30 μm fibre; chromatography was performed on either a 30 m ×0.25 mm id×0.25 μm ZB-WAX column (Phenomenex, UK). Samples were analyzed in triplicate.

Results – Preliminary data analysis of the full scan acquisition allowed the identification of 47 volatile compounds in wine samples. Tentative compound identification was based on at least 70% quality match with NIST 17 database information for each compound. In addition, experimental Retention Indexes were calculated and compared with the theoretical ones. Among the identified compounds we find acids, esters, alcohols and some terpenes. More detailed data analysis is necessary to identify the differences on wines aroma compounds produced starting from different treated vineyard and to understand the influence of the soil composition on wine characteristics.

DOI:

Publication date: September 27, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Giulia CHITARRINI1*, Maximilian LÖSCH2, Barbara RAIFER2, Peter ROBATSCHER1

1 Laboratory for Flavours and Metabolites, Institute for Agrochemistry and Food Quality, Laimburg Research Centre, Laimburg 6, 39040 Auer, Italy
2 Physiology and Cultivation Techniques, Institute for fruit Growing and Viticulture, Laimburg Research Centre, Laimburg 6, 39040 Auer, Italy

Contact the author

Keywords

grapevine, biochar, pyrogenic carbon, VOCs, GC-MS

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

SKIN AND SEED EXTRACTS DIFFERENTLY BEHAVE TOWARDS SALIVARY PROTEINS

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" text_orientation="center" custom_margin="65px||18px||false|false"...

GC-O and olfactoscan approaches to reveal premature aging markers in Chardonnay wine

Molecular markers of wine oxydation, such as sotolon or Strecker’s aldehydes that induce respectively nut or curry and boiled vegetables or wilted rose odors, can be percieved as a default by consumers. These volatile compounds are especially formed during the premature aging of wine, but it is likely that several contributing compounds are still unknown as is their combined contribution. This study was carried out to identify the markers of oxydation in Chardonnay wine by Gas Chromatography Olfactometry (GC-O) and to study the impact of these markers on the complex wine aromatic buffer using the Olfactoscan approach.A Chardonnay wine (2018-vintage), taken after malolactic fermentation without sulphites addition, was submitted to an artificial oxidation to simulate more or less prononced premature oxidation. Volatile compounds were extracted by Solid-Phase Extraction (SPE) and analysed by GC-O with a panel of 13 trained subjects. The same extract was also submitted to a second analysis based on the Olfactoscan technique, which allowed to evaluate the impact of each volatile compounds on the complex aromatic buffer of a non-oxidized wine delivered as background odor. Preliminary results revealed three types of behavior. On the one hand, several odor zones appeared only with the background odour, suggesting a synergy effect induced by the compounds in the aromatic buffer. Conversely, odor-active compounds could not be perceived within the background odor suggesting a masking effect. Finally several compounds were found to contribute as key odorants for wine oxydation once mixed with the aromatic buffer. These compounds are still to be identified using complementary techniques.

Estimating bulk stomatal conductance of grapevine canopies

In response to changes in their environment, grapevines regulate transpiration using various physiological mechanisms that alter conductance of water through the soil-plant-atmosphere continuum. Expressed as bulk stomatal conductance at the canopy scale, it varies diurnally in response to changes in vapor pressure deficit and net radiation, and over the season to changes in soil water deficits and hydraulic conductivity of both soil and plant. It is necessary to characterize the response of conductance to these variables to better model how vine transpiration also responds to these variables. Furthermore, to be relevant for vineyard-scale modeling, conductance is best characterized using data collected in a vineyard setting. Applying a crop canopy energy flux model developed by Shuttleworth and Wallace, bulk stomatal conductance was estimated using measurements of individual vine sap flow, temperature and humidity within the vine canopy, and estimates of net radiation absorbed by the vine canopy. These measurements were taken on several vines in a non-irrigated vineyard in Bordeaux France, using equipment that did not interfere with ongoing vineyard operations. An inverted Penman-Monteith equation was then used to calculate bulk stomatal conductance on 15-minute intervals from July to mid-September 2020. Time-series plots show significant diurnal variation and seasonal decreases in conductance, with overall values similar to those in the literature. Global sensitivity analysis using non-parametric regression found transpiration flux and vapor pressure deficit to be the most important input variables to the calculation of bulk stomatal conductance, with absorbed net radiation and bulk boundary layer conductance being much less important. Conversely, bulk stomatal conductance was one of the most important inputs when calculating vine transpiration, further emphasizing the need for characterizing its response to environmental changes for use in vineyard water use modeling.

Fleurtai, Soreli and Tocai Friulano: perspectives for quality integration of wine together with protection of the DOCG Lison Classico appellation

In modern viticulture, sustainability must be considered not only into the winery, but in the vineyard as well, being that with the most attentive interventions in order to protect the environment. In this context, the new “fungi resistant” varieties represent a valid option for reducing the negative environmental impact of agrochemicals used in viticulture, including those ones used in organic farming (given the copper accumulation into soils). Several application studies have demonstrated the enological validity of many resistant varieties, both in price and as a blend. Also, under the production point of view, the feasibility and economical sustainability of the new resistant varieties was verified. The aim of this work was to deepen the knowledge on the organoleptic characteristics of wines obtained from the Fleurtai and Soreli varieties and to compare them with the wine obtained from Tocai Friulano, the mother variety in the area destined for the production of the Lison Classico DOCG appellation. The purpose of the work is then to verify the possibility of introducing resistant varieties into the DOCG while maintaining the wine name of the appellation linked to the territory.

Effect of concentration and competition between different fungicide residues on the adsorption efficiency of activated vegetal fibres for treatment of wine

Vineyards are strongly exposed to fungal diseases, attacks from insects and competition with weeds. Most treatments used on grape vines contain synthetic active substances, which may be transferred to the wine. Such pesticides have a negative image because many active substances are potential health hazards. A specific oenological treatment allowing the reduction of pesticide residues in wine based on activated vegetable fibres (AVF) is under examination by the International Organisation for Vine and Wine. This technique works efficiently and alters the wine only little (Lempereur et al. 2014).