Influence of viticulture on the temporary evolution of the landscape: the case of the AO Ribera del Duero (Central Spain)

The effect of two rootstocks of different drought tolerance (1103 Paulsen and 3309 Couderc) on sap flow, water relations and gas exchange of cv. Xinomavro (Vitis vinifera L.) was investigated during the 2005 season in Naoussa, Greece. Soil was maintained at field capacity for both rootstock treatments until mid July when a restricted water regime was applied by irrigation cutoff. Sap flow diurnals for the Xinomavro-1103P combination showed a rapid decrease of flow after midday, under water stress conditions.

Il Nebbiolo, uno dei vitigni più rappresentativi della viticoltura piemontese. é caratterizzato da una maturazione tardiva, una elevata vigoria e una bassa fertilità basale. La sua popolazione inoltre presenta una tale variabilità morfologica che é consuetudine suddividere il vitigno in diverse sottovarietà (Lampia, Rosé, Michet, Balla per citare solo quelle dell’areale albese) ognuna con presunte distinte caratteristiche morfologiche e produttive.

The definition of soil health is complex due to the lack of agreement on adequate indicators and to the high variability of global soils. Nevertheless, it has been widely used as synonymous of soil quality for more than one decade, and there is a consensus warning of scientists that soil quality and biodiversity loss are occurring due to the traditional intensive agricultural practices.
In this work we monitored a set of soil parameters, both physicochemical and microbiological, in an experimental vineyard under three different management and land use systems: a) addition of external organic matter (EOM) to tilled soil; b) no tillage and plant cover between grapevine rows, and c) grapevines planted in rows running down the slope and tilled soil.

Epidermal Patterning Factors are a class of cysteine rich peptides known to be involved in many developmental processes. The role of EPF1, EPF2 and EPFL9 in controlling leaf stomata formation has been well described in model plants and cereals, and recently also in grapevine, while little is known about their activity in other organs. The aim of our study is to investigate whether VviEPFL9-2 can have a specific biological function in grapevine roots, where it resulted to be expressed. As grapevine is cultivated in the form of a grafted plant, we focused our study on the commonly used rootstock Kober 5BB (Vitis berlandieri x Vitis riparia). VviEPFL9-2 was edited in Kober 5BB plants using Agrobacterium tumefaciens transformation of embryogenic calli and the CRISPR/Cas9 technology. The phenotypic evaluation in greenhouse indicated that, as expected, the leaves of knock-out (KO) plants have a significant lower stomatal density compared to WT, associated with a lower stomatal conductance.

Context and purpose of the study. Conventional viticulture relies heavily on synthetic inputs (fertilizers, pesticides), as well as mechanization to manage pests, weeds, and diseases and maximize yields.