OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Grape and wine microorganisms: diversity and adaptation 9 Genetic causes of SO2 consumption in Saccharomyces cerevisiae

Genetic causes of SO2 consumption in Saccharomyces cerevisiae

Abstract

SO2 is used during winemaking for its anti-oxidative and anti-microbial properties. A high SO2 concentration in the wine has negative impacts by hiding wine aromas and delaying malolactic fermentation. SO2 concentration is also a source of health concerns and is therefore legally regulated. During the alcoholic fermentation SO2 can be produced or consumed by the yeast Saccharomyces cerevisiae with a high variability depending on the strain that accomplish the fermentation. The selection of industrial strains leaving less SO2 at the end of fermentation is therefore of great interest. 

In this study we implemented a QTL (Quantitative Trait Loci) mapping program to identify genetic factors that impact SO2 production by yeast during fermentation. This approach requires the study of a large progeny in segregation that must be characterized genetically and phenotypically. The establishment of a statistical link between genotype and phenotype allows the localization of QTLs that have an impact on the characters. 

Small-scale fermentations in 10 ml screw cap vessels coupled with robotized enzymatic allowed us to measure SO2 profile of several hundred individuals from two progenies. These two progenies were also genotyped by whole genome sequencing providing saturated genetic maps of thousands of markers. This experimental design led us to the identification of nine QTLs controlling SO2. Four of them present in MCH1, STR2 and SSU1 genes were molecularly validated. These alleles also show a pleiotropic effect with link between the production of SO2 and acetic acid. In the future, these new alleles can be used in cross breeding programs for the improvement of industrial strains.

DOI:

Publication date: June 10, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Emilien Peltier (1,2), Maria Martí Raga (1,3), Miguel Roncoroni (4), Vladimir Jiranek (5), Yves Gibon (4), Philippe Marullo (1,2)

(1) Unité de recherche Oenologie, EA 4577, USC 1366 INRA, ISVV, Université de Bordeaux, Bordeaux INP, F33882 Villenave d’Ornon France
(2) Biolaffort, Bordeaux, France
(3) Departament de Bioquímica i Biotecnologia, Facultat d’Enologia de Tarragona, Spain
(4) Wine Science Programme, University of Auckland, New Zealand
(5) Department of Wine and Food Science, University of Adelaide, Australia
(6) INRA, University of Bordeaux, UMR 1332 Fruit Biology and Pathology, Villenave d’Ornon, France

Contact the author

Keywords

Yeast, QTL mapping, SO2 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

The South African vineyard landscapes: impact on long term cultural practices

This paper follows the one presented by Saayman at the International Symposium on Landscapes of Vines and Wines in the Loire Valley during July 2003. Where Saayman’s paper described the heritage and development of South African vineyard landscapes, this one focuses on how the landscape is used to assist in decision-making concerning the most important long term practices.

Grape phylloxera leaf-feeding populations in commercial vineyards – a new biotype ?

Grape Phylloxera (Daktulosphaira vitifoliae Fitch) ordinarily has great difficulty establishing leaf galls on the European Grapevine (VitisviniferaL.). Yet populations of leaf-feeding Phylloxera are increasingly being observed throughout commercial vineyards world-wide. Effective plant protection strategies including quarantine actions are currently missing to fight, grape phylloxera populations in affected vineyards and combat linked negative effects on vines and yield. Contrary to the otherwise mandatory continuous infestation pressure from externally established populations (e.g. from populations developed on rootstock foliage or other interspecific hybrids, these leaf-feeding populations seem to establish themselves annually.

Reduced fungicide sprayings: A biodiversity boost?

Pesticides are considered one of the main causes for arthropod decline in agriculture which in turn may affect ecosystem services such as natural pest control and soil fertility.

Biomass carbon and nitrogen input from cover crops in an irrigated vineyard in Okanagan Valley, Canada

The use of cover crops in vineyards has been encouraged by positive effects on wine grape yield and sensory attributes, and improved soil function. This study examined the efficacy of three alleyway and three undervine cover crop treatments in an organic vineyard in the semiarid Okanagan Valley, Canada in 2021.

An overview of the impact of clone, environmental factors and viticultural techniques on rotundone concentration in red wines

Rotundone is the main aroma compound responsible for peppery notes in red wine. This positive and very potent molecule has an odor threshold of 8 ng/L in water and 16 ng/L in red wine. It has been detected in several grape varieties with some of the highest concentrations recorded in Syrah, Duras, Tardif and Noiret, an interspecific hybrid grown in the North-East of the USA. If several winemaking practices have been identified to lower rotundone in wine, up to date, no enological solution has proved its efficiency to maximize it. This means that efforts to produce high rotundone wines must be undertaken in vineyards. This work provides practical ways that can be used by winegrowers to modulate rotundone levels in their wines.