Use of antisense RNA technology to modulate gene expression in Œnococcus oeni

Pruning vine-shoots are a viticulture waste that have been traditionally poorly exploited in relation to its chemical minority composition related to phenolic and volatile compounds. In this line, toasted vine-shoots supposes a proposal of enological tool to use to modulate the chemical and sensorial profile of wines. From a phenolic point of view, when vine-shoots are used during winemaking mainly influence to increase the flavanols and stilbenes content, mostly trans-resveratrol, as also an increasing in the sweet tannins and decreasing the green character and total anthocyanins, changing the violet for garnet colour.

Volatile thiols are impact aroma compounds, well-known in the literature for imparting tropical fruit aromas such as passion fruit, guava, grapefruit, and citrus in white wines [1]. More recent evidence suggests that tropical fruit aromas are also caused by other aroma compounds besides thiols, such as fermentation esters, or the interaction between these volatile families. Therefore, the objective of this study was to investigate the effects of combining esters and/or thiols to determine their impact on the fruitiness aroma perception of white wines. Pinot gris wine was produced at the OSU research winery and was dearomatized using Lichrolut® EN. Combinations of fermentation volatile compounds were added to the wine, forming the aroma base. Treatment wines were composed of additions of different concentrations and combinations of thiols and/or esters. Samples were subjected to sensory analysis where forty-six white wine consumers evaluated the orthonasal aroma of the wines and participated in Check-All-That-Apply (CATA).

Phosphonic acid and especially potassium dihydrogen phosphonate are widely used to restrain the ubiquitous pressure of grapevine downy mildew in viticulture. Nevertheless, phosphonic acid and its derivatives have been banned in organic viticulture in October 2013, because they have been classified as plant protection products since then.

Driven by the demand for sustainable agriculture, biocontrol is emerging as a crucial alternative to chemical fungicides for crop protection.

Aims: The aim of the study was to investigate whether organic or biodynamic management have a long-term impact on 1) the microbial biomass and enzymatic activity in the soil, 2) the soil microbial community, 3) flying as well as soil living arthropods and associated fungi.