GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Residual copper quantification on grapevine’s organs

Residual copper quantification on grapevine’s organs

Abstract

Context and purpose of the study – Copper is listed among the active substances candidates for substitution (Regulation EU 2015/408). Yet still, because of the lack of valid alternatives, the European Commission recently confirmed its usage authorization by limiting the maximum amount to 28 Kg per hectare in 7 years, i.e. an average of 4 kg/year (Reg. EU 2018/1981).This restriction is due to copper accumulation in soils and surface waters both caused by a steady application, especially on perennial crops (Riepert et al., 2013). The aim of this work is to determine if treatments with reduced copper dosages are able to reach different grapevine’s organs, with particular focus on the core of bunches, and if these small amounts can ensure the respect of the legislative prescription, without compromising the phytosanitary conditions of the vineyards, thus grape yields.

Material and methods – Samples from Incrocio Manzoni and Pinot noir varieties, grown in two different farms and training systems, were collected in four repetitions twice a month, between fruit set and veraison. Each leaf sample was prepared by obtaining 90 foliar discs of 2.7 cm diameter. Berries were divided according to their positions on the bunch and referring to the sprayer flow: internal, directly and indirectly exposed. Rachis were classified in first, second and third degree, i.e. main axis, first and last brunch respectively. Samples were washed with a 1% nitric acid solution and analyzed for copper quantification with an optical ICP. Leaves surface was obtained by applying a geometrical formula, while rachis and berries were measured after washing by scanning their images with the software ImageJ. Thus, approximating rachis to cilinders and berries to prolate spheroids, geometrical parameters were determined in order to calculate their 3D surface. Variance analysis (ANOVA) and Tukey’s test were performed (p<0,05, software “Dell™ Statistica™ 13.0”).

Results – The most copper content per surface unit was observed on the leaves: double amount (between 23 and 47 mg/m2) if compared to rachis (between 9 and 22 mg/m2) and triple as much when referring to internal and indirectly exposed berries (between 2 and 10 mg/m2). Values on rachis were higher on the terminal portions (2nd and 3rd degree) suggesting an elution phenomenon of the berries superficial copper and its further penetration inwards the cluster. Considering berries, the directly exposed ones carried the most amount of copper, while internal and indirectly exposed berries showed similar accumulation dynamics, pointing out the same difficulty in terms of spray distribution. Moreover, in one farm values only reached the efficacy range against Plasmopora viticola (5-10 mg/m2)(CABÚS et al., 2017) around veraison, when stomata are already closed. This could be explained through the lower total Cu amount sprayed on Incrocio Manzoni (3,4 kg) in relation to Pinot noir (5,5 kg) over the season. This study ascertains a copper accumulation over the season despite the run off caused by rainfalls and shows that treatments actually reach the most sensitive parts of the grapevine. Nevertheless, in farms using a strategy with reduced Cu dosages, some lacks in crop’s coverage could occur.According to the new legislative directives (Reg. EU 2018/1981), the limit of 28 kg/hectare in 7 years means an average of 4 kg/hectare/year, which could lead to limit situations as seen in this work. The intervention timing and a proper canopy management increase thus their importance as preparatory techniques for Cu efficacy.

DOI:

Publication date: September 21, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Silvia GUGOLE, Roberto ZANZOTTI, Daniela BERTOLDI, Enzo MESCALCHIN

Fondazione Edmund Mach, 1 Via Edmund Mach, 38010 San Michele all’Adige, Trento, Italia

Contact the author

Keywords

grapevine, organic viticulture, copper, treatments, berries, rachis, leaves

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

The impact of sustainable management regimes on amino acid profiles in grape juice, grape skin flavonoids, and hydroxycinnamic acids

One of the biggest challenges of agriculture today is maintaining food safety and food quality while providing ecosystem services such as biodiversity conservation, pest and disease control, ensuring water quality and supply, and climate regulation. Organic farming was shown to promote biodiversity and carbon sequestration, and is therefore seen as one possibility of environmentally friendly production. Consumers expect organically grown crops to be free from chemical pesticides and mineral fertilizers and often presume that the quality of organically grown crops is different or higher compared to conventionally grown crops. Integrated, organic, and biodynamic viticulture were compared in a replicated field trial in Geisenheim, Germany (Vitis vinifera L. cv. Riesling). Amino acid profiles in juice, grape skin flavonoids, and hydroxycinnamic acids were monitored over three consecutive seasons beginning 7 years after conversion to organic and biodynamic viticulture, respectively. In addition, parameters such as soil nutrient status, yield, vigor, canopy temperature, and water stress were monitored to draw conclusions on reasons for the observed changes. Results revealed that the different sustainable management regimes highly differed in their amino acid profiles in juice and also in their skin flavonol content, whereas differences in the flavanol and hydroxycinnamic acid content were less pronounced. It is very likely that differences in nutrient status and yield determined amino acid profiles in juice, although all three systems showed similar amounts of mineralized nitrogen in the soil. Canopy structure and temperature in the bunch zone did not differ among treatments and therefore cannot account for the observed differences in favonols. A different light exposure of the bunches in the respective systems due to differences in vigor together with differences in berry size and a different water status of the vines might rather be responsible for the increase in flavonol content under organic and biodynamic viticulture.

Combining effect of leaf removal and natural shading on grape ripening under two irrigation strategies in Manto negro (Vitis vinifera L.)

The increasingly frequent heat waves during grape ripening pose challenges for high quality wine grape production. Defoliation is a common practice that can improve the control of diseases in bunches, but also it increases the exposure to sunlight. Grapes exposed to solar radiation reach temperatures over the optimum for berry development and maturation. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 using Manto negro wine grapes to study the effect of applied irrigation and different light exposure levels on grape quality. Two irrigation treatments were imposed based on the frequency and amount of water doses in a four-block experimental vineyard at Bodega Ribas (Mallorca). Three light exposure treatments were randomly applied in each irrigation plot. The light treatments included exposed clusters from pea size, non-exposed clusters, and shaded clusters after softening. Leaf area index and canopy porosity was estimated every 2 weeks. Midday leaf water potential was measured weekly. Additionally, apparent electrical conductivity was measured between rows to estimate the soil water content variability. Light and temperature sensors were installed at the bunch level to quantify the differences in bunch temperature and light intensity among treatments. The effect of irrigation and cluster light exposure on berry weight, TSS, TA, malic acid, tartaric acid, K+, and pH were analysed at 5 moments along grape ripening. During different heat waves, the natural shading technique decreased the maximum bunch temperature around 10 °C respect to the exposed bunches in both irrigation strategies. The combination of defoliation and shading techniques after softening decreased TSS at harvest and affected most of the quality parameters during the last stages of ripening, showing an interesting technique to delay ripening in warm viticulture areas.

Short-term relationships between climate and grapevine trunk diseases in southern French vineyards

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Evaluation of climate change impacts at the Portuguese Dão terroir over the last decades: observed effects on bioclimatic indices and grapevine phenology

In the last decades the growers of the Portuguese Dão winegrowing region (center of Portugal) are experiencing changes in climate that are influencing either grape phenology berry health and ripening. Aiming to study the relationships between climate indices (CI), seasonal weather and grapevine phenology, in this work long-term climate and phenological data collected at the experimental vineyard of the Portuguese Dão research centre between 1958 and 2019 (61 years) for the red variety Touriga Nacional, was analyzed. The trends over time for the classical temperature-based indices (Growing Season Temperature – GST -, Growing Degree Days – GDD, Huglin Index – HI and Cool Night Index – CI) presented a significantly positive slope while the Dryness Index (DI) showed a negative trend over the last 61 years. Regarding grapevine phenology, an average advance of 4.5 days per decade in the harvest day was observed throughout the last 61 years. Consequently, the weather conditions during the ripening period have changed, showing an increasing trend over time in the average temperature (higher magnitude in the maximum than in the minimum temperature) and a decrease in the accumulated rainfall. A regression analysis showed that ~50% of harvest date variability over years was explained by the temperature-based indices variability. These observed effects of climate change on bioclimatic indices and corresponding anticipation of harvest date can still be considered advantageous for the Dão terroir as it allows to achieve an optimal berry ripening before the common equinox rains and, therefore, avoid the potential negative impacts of the rainfall on berry health and composition.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…