GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Residual copper quantification on grapevine’s organs

Residual copper quantification on grapevine’s organs

Abstract

Context and purpose of the study – Copper is listed among the active substances candidates for substitution (Regulation EU 2015/408). Yet still, because of the lack of valid alternatives, the European Commission recently confirmed its usage authorization by limiting the maximum amount to 28 Kg per hectare in 7 years, i.e. an average of 4 kg/year (Reg. EU 2018/1981).This restriction is due to copper accumulation in soils and surface waters both caused by a steady application, especially on perennial crops (Riepert et al., 2013). The aim of this work is to determine if treatments with reduced copper dosages are able to reach different grapevine’s organs, with particular focus on the core of bunches, and if these small amounts can ensure the respect of the legislative prescription, without compromising the phytosanitary conditions of the vineyards, thus grape yields.

Material and methods – Samples from Incrocio Manzoni and Pinot noir varieties, grown in two different farms and training systems, were collected in four repetitions twice a month, between fruit set and veraison. Each leaf sample was prepared by obtaining 90 foliar discs of 2.7 cm diameter. Berries were divided according to their positions on the bunch and referring to the sprayer flow: internal, directly and indirectly exposed. Rachis were classified in first, second and third degree, i.e. main axis, first and last brunch respectively. Samples were washed with a 1% nitric acid solution and analyzed for copper quantification with an optical ICP. Leaves surface was obtained by applying a geometrical formula, while rachis and berries were measured after washing by scanning their images with the software ImageJ. Thus, approximating rachis to cilinders and berries to prolate spheroids, geometrical parameters were determined in order to calculate their 3D surface. Variance analysis (ANOVA) and Tukey’s test were performed (p<0,05, software “Dell™ Statistica™ 13.0”).

Results – The most copper content per surface unit was observed on the leaves: double amount (between 23 and 47 mg/m2) if compared to rachis (between 9 and 22 mg/m2) and triple as much when referring to internal and indirectly exposed berries (between 2 and 10 mg/m2). Values on rachis were higher on the terminal portions (2nd and 3rd degree) suggesting an elution phenomenon of the berries superficial copper and its further penetration inwards the cluster. Considering berries, the directly exposed ones carried the most amount of copper, while internal and indirectly exposed berries showed similar accumulation dynamics, pointing out the same difficulty in terms of spray distribution. Moreover, in one farm values only reached the efficacy range against Plasmopora viticola (5-10 mg/m2)(CABÚS et al., 2017) around veraison, when stomata are already closed. This could be explained through the lower total Cu amount sprayed on Incrocio Manzoni (3,4 kg) in relation to Pinot noir (5,5 kg) over the season. This study ascertains a copper accumulation over the season despite the run off caused by rainfalls and shows that treatments actually reach the most sensitive parts of the grapevine. Nevertheless, in farms using a strategy with reduced Cu dosages, some lacks in crop’s coverage could occur.According to the new legislative directives (Reg. EU 2018/1981), the limit of 28 kg/hectare in 7 years means an average of 4 kg/hectare/year, which could lead to limit situations as seen in this work. The intervention timing and a proper canopy management increase thus their importance as preparatory techniques for Cu efficacy.

DOI:

Publication date: September 21, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Silvia GUGOLE, Roberto ZANZOTTI, Daniela BERTOLDI, Enzo MESCALCHIN

Fondazione Edmund Mach, 1 Via Edmund Mach, 38010 San Michele all’Adige, Trento, Italia

Contact the author

Keywords

grapevine, organic viticulture, copper, treatments, berries, rachis, leaves

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Phenological characterization of a wide range of Vitis Vinifera varieties

In order to study the impact of climate change on Bordeaux grape varieties and to assess the adaptation capacities of candidates to the grape varieties of this wine region to the new climatic conditions, an experimental block design composed of 52 grape varieties was set up in 2009 at the INRAE Bordeaux Aquitaine center. Among the many parameters studied, the three main phenological stages of the vine (budburst, flowering and veraison) have been closely monitored since 2012. Observations for each year, stage and variety were carried out on four independent replicates. Precocity indices have been calculated from the data obtained over the 2012-2021 period (Barbeau et al. 1998). This work allowed to group the phenological behaviour of the grapevine varieties, not only based on the timing of the subsequent developmental stages, but also on the overall precocity of the cycle and the total length of the cycle between budburst and veraison. Results regarding the variability observed among the different grape varieties for these phenological stages are presented as heat maps.

The rootstock, the neglected player in the scion transpiration even during the night

Water is the main limiting factor for yield in viticulture. Improving drought adaptation in viticulture will be an increasingly important issue under climate change. Genetic variability of water deficit responses in grapevine partly results from the rootstocks, making them an attractive and relevant mean to achieve adaptation without changing the scion genotype. The objective of this work was to characterize the rootstock effect on the diurnal regulation of scion transpiration. A large panel of 55 commercial genotypes were grafted onto Cabernet Sauvignon. Three biological repetitions per genotype were analyzed. Potted plants were phenotyped on a greenhouse balance platform capable of assessing real-time water use and maintaining a targeted water deficit intensity. After a 10 days well-watered baseline period, an increasing water deficit was applied for 10 days, followed by a stable water deficit stress for 7 days. Pruning weight, root and aerial dry weight and transpiration were recorded and the experiment was repeated during two years. Transpiration efficiency (ratio between aerial biomass and transpiration) was calculated and δ13C was measured in leaves for the baseline and stable water deficit periods. A large genetic variability was observed within the panel. The rootstock had a significant impact on nocturnal transpiration which was also strongly and positively correlated with maximum daytime transpiration. The correlations with growth and water use efficiency related traits will be discussed. Transpiration data were also related with VPD and soil water content demonstrating the influence of environmental conditions on transpiration. These results highlighted the role of the rootstock in modulating water deficit responses and give insights for rootstock breeding programs aimed at identifying drought tolerant rootstocks. It was also helpful to better define the mechanisms on which the drought tolerance in grapevine rootstocks is based on.

The impact of sustainable management regimes on amino acid profiles in grape juice, grape skin flavonoids, and hydroxycinnamic acids

One of the biggest challenges of agriculture today is maintaining food safety and food quality while providing ecosystem services such as biodiversity conservation, pest and disease control, ensuring water quality and supply, and climate regulation. Organic farming was shown to promote biodiversity and carbon sequestration, and is therefore seen as one possibility of environmentally friendly production. Consumers expect organically grown crops to be free from chemical pesticides and mineral fertilizers and often presume that the quality of organically grown crops is different or higher compared to conventionally grown crops. Integrated, organic, and biodynamic viticulture were compared in a replicated field trial in Geisenheim, Germany (Vitis vinifera L. cv. Riesling). Amino acid profiles in juice, grape skin flavonoids, and hydroxycinnamic acids were monitored over three consecutive seasons beginning 7 years after conversion to organic and biodynamic viticulture, respectively. In addition, parameters such as soil nutrient status, yield, vigor, canopy temperature, and water stress were monitored to draw conclusions on reasons for the observed changes. Results revealed that the different sustainable management regimes highly differed in their amino acid profiles in juice and also in their skin flavonol content, whereas differences in the flavanol and hydroxycinnamic acid content were less pronounced. It is very likely that differences in nutrient status and yield determined amino acid profiles in juice, although all three systems showed similar amounts of mineralized nitrogen in the soil. Canopy structure and temperature in the bunch zone did not differ among treatments and therefore cannot account for the observed differences in favonols. A different light exposure of the bunches in the respective systems due to differences in vigor together with differences in berry size and a different water status of the vines might rather be responsible for the increase in flavonol content under organic and biodynamic viticulture.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Simulating climate change impact on viticultural systems in historical and emergent vineyards

Global climate change affects regional climates and hold implications for wine growing regions worldwide. Although winegrowers are constantly adapting to internal and external factors, it seems relevant to develop tools, which will allow them to better define actual and future agro-climatic potentials. Within this context, we develop a modelling approach, able to simulate the impact of environmental conditions and constraints on vine behaviour and to highlight potential adaptation strategies according to different climate change scenarios. Our modeling approach, named SEVE (Simulating Environmental impacts on Viticultural Ecosystems), provides a generic modeling framework for simulating grapevine growth and berry ripening under different conditions and constraints (slope, aspect, soil type, climate variability…) as well as production strategies and adaptation rules according to climate change scenarios. Each activity is represented by an autonomous agent able to react and adapt its reaction to the variability of environmental constraints. Using this model, we have recently analyzed the evolution of vineyards’ exposure to climatic risks (frost, pathogen risk, heat wave) and the adaptation strategies potentially implemented by the winegrowers. This approach, implemented for two climate change scenarios, has been initiated in France on traditional (Loire Valley) and emerging (Brittany) vineyards. The objective is to identify the time horizons of adaptations and new opportunities in these two regions. Carried out in collaboration with wine growers, this approach aims to better understand the variability of climate change impacts at local scale in the medium and long term.