GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Essential oil vapor triggers resistance pathways in Vitis vinifera and blocks plasmopora viticola infection

Essential oil vapor triggers resistance pathways in Vitis vinifera and blocks plasmopora viticola infection

Abstract

The amount of synthetic pesticides applied in viticulture is relatively high compared to other agricultural crops, due to the high sensitivity of grapevine to diseases such as downy mildew (Plasmopora viticola). Alternatives to reduce fungicides are utterly needed to promote a sustainable vineyard-ecosystems and meet consumer acceptance.
Essential oils (EOs) are amongst the most promising natural plant protection agents and have shown their antifungal properties previously. However, the efficiency of EOs depends highly on timing and application technique. Additionally, the molecular interactions of host, pathogen and EO, which underlie the efficiency of EOs, are not understood. The presented study aimed to a) evaluate whether a continuous fumigation of EO can control downy mildew and b) decipher molecular mechanisms triggered in host and pathogen by EO. A custom made climatic chamber was constructed, which enabled a continuous fumigation of vines with different EOs during long term experiments.
Several experiments were carried out with vine cuttings infected with Plasmopora viticola and subsequently exposed to continuous fumigation of different EOs with different concentrations and application times (24 h to 10 d). Experiments were stopped when infection symptoms were clearly present on the control. Physiological parameters (photosynthesis, growth rate) were recorded and leaves were sampled at different time points for subsequent RNA extraction.
The post-infection oregano oil vapor treatment during 24h was sufficient to reduce downy mildew development to 95%. Leaf RNA sampled after 24 hours and 10 days of EO treatment was used for RNA-seq analysis. Sequenced reads were mapped onto the Vitis vinifera and Plasmopora viticola genomes. Less than 1% of reads could be mapped onto the Plasmopora genome from treated samples, whereas up to 30 % reads mapped from the controls, thereby confirming visual observation of P. viticola absence under treatment. An average of 80 % reads could be mapped onto the V. vinifera genome for differential expression analysis, which yielded 4800 modulated transcripts. Grapevine genes triggered by EO treatment were mainly linked to plant biotic stress response and plant-pathogen interactions. Key genes controlling ethylene synthesis, phenylpropanoids and flavonoid synthesis were also highly activated by EO. We report here for the first time the effects of EO treatments on the control of a grapevine pathogen, concomitantly with the molecular description of EO-host-pathogen interactions. These results strongly support the hypothesis that the antifungal efficiency of EO is indirect and mainly due to switching on resistance pathways of the host plants. These results are of major importance for the production and research on biopesticides, plant stimulation products as well as for resistance breeding strategies.

DOI:

Publication date: September 26, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Markus RIENTH1*, Sana GHAFFARI1, Marylin CLÉROUX1, Arnaud PERNET1, Julien CROVADORE3, Eric REMOLIF2 Jean-Philipp BURDET1, Francois LEFORT3

1 Changins, HES-SO University of Applied Sciences and Arts Western Switzerland, route de Duillier 60, 1260 Nyon, Switzerland
2 Agroscope, route de Duillier 50, 1260 Nyon, Switzerland
3 HEPIA, HES-SO University of Applied Sciences and Arts Western Switzerland, Jussy, Switzerland

Contact the author

Keywords

plant defense, essential oil, Plasmopara viticola, grapevine

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…

Effect of fertigation strategies to adapt PGI Côtes de Gascogne production to hot vintage

The development of fertigation could be a possible solution to adapt PGI Côtes de Gascogne (south-western France) wine production to climate change. The goal would be to limit the negative effects of water stress on yield performance expectation (around 15 tons per hectare) and to make the use of fertilizers more efficient. This study aimed to compare the effects of three strategies of water and minerals supply on grapes and wines qualities. Two fertigation practices were compared to a rainfed control which is the current standard of the local grape growing production. The fertilizers (nitrogen and potassium) were (i) fully brought by irrigation pipe during the season, (ii) partially brought by irrigation pipe and partially on the soil or (iii) fully brought on the soil at the beginning of the season for the non-irrigated control (local standard). The trial was run on cv. Colombard trained on spur pruned with vertical shoot positioning system on a sandy-silty-clay soil over the 2020 vintage which was particularly hot for the region. Moderate to strong water deficit appeared during the growing period of the berries and held on after veraison. Irrigation strategies allowed for maintaining grapevine without water deficit and being significantly different from the control water status. Grapevine with fully or partial fertigation strategies produced 25% more yield mainly due to the increase of the bunch weight. Also, the fully fertigation showed the best ratio between yield and maturity and brought 30% less of fertilizers (both nitrogen and potassium) than the two other strategies. Finally, the analysis of aromatic compounds in Colombard wines, varietal thiols family, showed the same level of concentrations for the 3 treatments, confirming that the yield performance did not impact the aromatic potential in this trial.

Legacy of land-cover changes on soil erosion and microbiology in Burgundian vineyards

Soils in vineyards are recognized as complex agrosystems whose characteristics reflect complex interactions between natural factors (lithology, climate, slope, biodiversity) and human activities. To date, most of the unknown lies in an incomplete understanding of soil ecosystems, and specifically in the microbial biodiversity even though soil microbiota is involved in many key functions, such as nutrient cycling and carbon sequestration. Soil biological properties are indicative of soil quality. Therefore, understanding how soil communities are related to soil ecosystem functioning is becoming an essential issue for soil strategy conservation. Here, we propose to assess the importance of land-cover history on the present-day microbiological and physico-chemical properties. The studied area was selected in the Burgundian vineyards (Pernand-Vergelesses, Burgundy, France) where land occupation has been reconstructed over the last 40 years. Soil samples were collected in five areas reflecting various land cover history (forest, vineyards, shifting from forest to vineyards). For each area, physico-chemical parameters (pH, C, N, P, grain size) were measured and DNA was extracted to characterize the abundance and diversity of microbial communities. The obtained results show significant differences in the five areas suggesting that present-day microbial molecular biomass and bacterial taxonomic is partly inherited from past land occupation. Over longer period of time, such study of land-uses legacies may help to better assess ecosystem recovery and the impact of management practices for a better soil quality and vineyards sustainability.

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).

Terroir analysis and its complexity

Terroir is not only a geographical site, but it is a more complex concept able to express the “collective knowledge of the interactions” between the environment and the vines mediated through human action and “providing distinctive characteristics” to the final product (OIV 2010). It is often treated and accepted as a “black box”, in which the relationships between wine and its origin have not been clearly explained. Nevertheless, it is well known that terroir expression is strongly dependent on the physical environment, and in particular on the interaction between soil-plant and atmosphere system, which influences the grapevine responses, grapes composition and wine quality. The Terroir studying and mapping are based on viticultural zoning procedures, obtained with different levels of know-how, at different spatial and temporal scales, empiricism and complexity in the description of involved bio-physical processes, and integrating or not the multidisciplinary nature of the terroir. The scientific understanding of the mechanisms ruling both the vineyard variability and the quality of grapes is one of the most important scientific focuses of terroir research. In fact, this know-how is crucial for supporting the analysis of climate change impacts on terroir resilience, identifying new promised lands for viticulture, and driving vineyard management toward a target oenological goal. In this contribution, an overview of the last findings in terroir studies and approaches will be shown with special attention to the terroir resilience analysis to climate change, facing the use and abuse of terroir concept and new technology able to support it and identifying the terroir zones.