GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Essential oil vapor triggers resistance pathways in Vitis vinifera and blocks plasmopora viticola infection

Essential oil vapor triggers resistance pathways in Vitis vinifera and blocks plasmopora viticola infection

Abstract

The amount of synthetic pesticides applied in viticulture is relatively high compared to other agricultural crops, due to the high sensitivity of grapevine to diseases such as downy mildew (Plasmopora viticola). Alternatives to reduce fungicides are utterly needed to promote a sustainable vineyard-ecosystems and meet consumer acceptance.
Essential oils (EOs) are amongst the most promising natural plant protection agents and have shown their antifungal properties previously. However, the efficiency of EOs depends highly on timing and application technique. Additionally, the molecular interactions of host, pathogen and EO, which underlie the efficiency of EOs, are not understood. The presented study aimed to a) evaluate whether a continuous fumigation of EO can control downy mildew and b) decipher molecular mechanisms triggered in host and pathogen by EO. A custom made climatic chamber was constructed, which enabled a continuous fumigation of vines with different EOs during long term experiments.
Several experiments were carried out with vine cuttings infected with Plasmopora viticola and subsequently exposed to continuous fumigation of different EOs with different concentrations and application times (24 h to 10 d). Experiments were stopped when infection symptoms were clearly present on the control. Physiological parameters (photosynthesis, growth rate) were recorded and leaves were sampled at different time points for subsequent RNA extraction.
The post-infection oregano oil vapor treatment during 24h was sufficient to reduce downy mildew development to 95%. Leaf RNA sampled after 24 hours and 10 days of EO treatment was used for RNA-seq analysis. Sequenced reads were mapped onto the Vitis vinifera and Plasmopora viticola genomes. Less than 1% of reads could be mapped onto the Plasmopora genome from treated samples, whereas up to 30 % reads mapped from the controls, thereby confirming visual observation of P. viticola absence under treatment. An average of 80 % reads could be mapped onto the V. vinifera genome for differential expression analysis, which yielded 4800 modulated transcripts. Grapevine genes triggered by EO treatment were mainly linked to plant biotic stress response and plant-pathogen interactions. Key genes controlling ethylene synthesis, phenylpropanoids and flavonoid synthesis were also highly activated by EO. We report here for the first time the effects of EO treatments on the control of a grapevine pathogen, concomitantly with the molecular description of EO-host-pathogen interactions. These results strongly support the hypothesis that the antifungal efficiency of EO is indirect and mainly due to switching on resistance pathways of the host plants. These results are of major importance for the production and research on biopesticides, plant stimulation products as well as for resistance breeding strategies.

DOI:

Publication date: September 26, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Markus RIENTH1*, Sana GHAFFARI1, Marylin CLÉROUX1, Arnaud PERNET1, Julien CROVADORE3, Eric REMOLIF2 Jean-Philipp BURDET1, Francois LEFORT3

1 Changins, HES-SO University of Applied Sciences and Arts Western Switzerland, route de Duillier 60, 1260 Nyon, Switzerland
2 Agroscope, route de Duillier 50, 1260 Nyon, Switzerland
3 HEPIA, HES-SO University of Applied Sciences and Arts Western Switzerland, Jussy, Switzerland

Contact the author

Keywords

plant defense, essential oil, Plasmopara viticola, grapevine

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Downscaling of remote sensing time series: thermal zone classification approach in Gironde region

In viticulture, the challenges of local climate modelling are multiple: taking into account the local environment, fine temporal and spatial scales, reliable time series of climate data, ease of implementation and reproducibility of the method. At the local scale, recent studies have demonstrated the contribution of spatialization methods for ground-based climate observation data considering topographic factors such as altitude, slope, aspect, and geographic coordinates (Le Roux et al, 2017; De Rességuier et al, 2020). However, these studies have shown questions in terms of the reproducibility and sustainability of this type of climate study. In this context, we evaluated the potential of MODIS thermal satellite images validated with ground-based climate data (Morin et al, 2020). Previous studies have been encouraging, but questions remain to be explored at the regional scale, particularly in the dynamics of the massive use of bioclimatic indices to classify the climate of wine regions. The results at the local scale were encouraging, but this approach was tested in the current study at the regional scale. Several objectives were set: 1) to evaluate the downscaling method for land surface temperature time series, 2) to identify regional thermal structure variations. We used weekly minimum and maximum surface temperature time series acquired by MODIS satellites at a spatial resolution of 1000 m and downscaled at 500 m using topographical variables. Two types of analyses were performed:

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.

The use of rootstock as a lever in the face of climate change and dieback of vineyard

As viticulture faces challenges such as climate change or vineyard dieback, the choice of the variety and rootstock becomes more and more crucial. To study rootstock levers in the Bordeaux region, a parcel of Cabernet Sauvignon (CS) was planted with four rootstocks in 2014. Twenty repetitions of each of the following four rootstocks were set up: 101-14 MGt, Nemadex AB, 420A MGt and Gravesac. The number of bunches, yields and pruning weights of the vine shoots were measured individually on 240 vines from 2017 to 2021. Since 2020, nitrogen status assessed by assimilable nitrogen level, hydric status assessed by δ13C and berry maturity were measured on 80 samples taken from 20 repetitions of the four rootstocks. A lower yield was measured for CS grafted onto Nemadex AB due to the lower number of bunches and the lower weight of berries. The differences between the other three rootstocks are small, but CS grafted onto 420A MGt was the most productive. The CS grafted onto Nemadex AB had the lowest pruning weight while 101-14 MGt had the highest. In 2020, δ13C showed a more moderate water stress with 101-14 MGt and 420A MGt than with Nemadex AB. Surprisingly, the Gravesac was under more stress than the 101-14 MGt. The nitrogen status in the berries was better for Nemadex AB but this was perhaps due to the significantly lower weight of the berries.Rootstock 101-14 MGt attained the highest accumulation of sugars in the berries while 420A MGt allows to preserve higher acidity. The parcel is still young which may explain some of the results. These measures must therefore be continued over the next several years to fully assess the effects of these rootstocks on the development of the vines and the quality of the production under new climatic conditions.

Under-vine management effects on grapevine production, soil properties and plant communities in South Australia

Under-vine (UV) management has traditionally consisted of synthetic herbicide use to limit competition between weeds and grapevines. With growing global interest towards non-synthetic chemical use, this study aimed to capture the effects of alternative UV management at two commercial Shiraz vineyards in South Australia, where the sole management variables were UV management since 2016. In adjacent treatment blocks, cultivation (CU) was compared to spontaneous vegetation (SV) in McLaren Vale (MV), and herbicide was compared to SV in Eden Valley (EV). Soil water infiltration rates were slower and grapevine stem water potential was lower in CU compared to SV in MV, with the latter having a plant community dominated by soursob (Oxalis pes-caprae) during winter; while in EV, there was little separation between the treatments. Yields were affected at both sites, with SV being higher in MV and HE being higher in EV. In MV, the only effect on grape must was a lower 13C:12C isotope ratio in CU, indicating greater grapevine water stress. In the grape must at EV, SV had higher total soluble solids, total phenolics, anthocyanins, and yeast available nitrogen; and lower pH and titratable acidity. Pruning weights were not affected by the treatments in MV, while they were higher in HE at EV. Assessments revealed that the differing soil types at the two sites were likely the main determinants of the opposing production outcomes associated with UV management. In the silty loam soil of MV, the higher yields in SV were likely due to more plant-available water, as a potential result of the continuous soil bio-pores formed by winter UV vegetation. Conversely, in the loamy sand soils of EV with a lower cation exchange capacity, the lower yields and pruning weights in SV suggest the UV vegetation competed significantly with the grapevines for available water and nutrients.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.