terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Identification of loci associated with specialised metabolites in Vitis vinifera

Identification of loci associated with specialised metabolites in Vitis vinifera

Abstract

Secondary (or specialised) metabolites such as terpenes and phenolic compounds are produced by plants for various roles which include defence against pathogens and herbivores, protection against abiotic stress, and plant signalling. Additionally, these metabolites influence grapevine quality traits such as colour, aroma, taste, and nutritional value. However, the biosynthesis of these metabolites is often complex and controlled by multiple genes which in grapevine are predominantly uncharacterised. This study therefore aimed to identify novel loci associated with grapevine volatile organic and phenolic compounds. Chemical analysis of these compound classes was performed via GC-MS and UPLC analysis in a grapevine mapping population, and the quantified metabolites used for quantitative trait loci (QTL) analysis.  Several significant QTLs associated with terpenes and phenolic compounds were identified, and the underlying genomic regions were investigated. For phenolic compounds, a novel locus associated with caftaric acid biosynthesis was identified, and a hydroxycinnamoyltransferase (VvHCT) was investigated as a candidate gene. Several terpene synthases (VvTPSs) co-localised with QTLs associated with monoterpenes and sesquiterpenes. Notably, loci on chromosomes 12 and 13 were shown to be associated with geraniol and cyclic monoterpene accumulation, respectively. The locus on chromosome 12 was shown to contain a geraniol synthase gene (VvGer), while the locus on chromosome 13 contained an a-terpineol synthase gene (VvTer). Further molecular and genomic investigation of VvGer and VvTer found that these genes appear in tandemly duplicated clusters, with high levels of hemizygosity which was further supported by genomic data from recently published diploid grapevine genomes. Interestingly, copy number analysis demonstrated that VvTer gene copy number correlated with both VvTerexpression and the accumulation of cyclic monoterpenes, highlighting the impact of VvTPS gene duplication and copy number variation on terpene accumulation in grapevine.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Robin Bosman*1 and Justin Graham Lashbrooke2

1South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, South Africa
2 Department of Genetics, Stellenbosch University, South Africa.

Contact the author*

Keywords

terpenes, TPS, grapevine, gene copy number, genomics, QTL, phenolics

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Genetic study of wild grapevines in La Rioja region

Since the mid-1980s, several surveys have been carried out in La Rioja to search for populations of the sylvestris grapevine subspecies (Vitis vinifera L. subsp. sylvestris Gmelin). The banks of the Ebro River and its tributaries (Alhama, Cidacos, Leza, Iregua, Najerilla, Oja and Tirón rivers), as well as the surrounding vegetation of their valleys have been covered. So far, all the populations found are alluvial, forming part of the riparian vegetation of the Najerilla (the first reported population in La Rioja [1]), Iregua, and the vicinity of Oja valleys.

Read More

Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Leaf removal in the fruit-zone has been employed to improve cluster light exposure and ventilation and therefore increase metabolite accumulation and reduce botrytis incidence in berries. When applied before flowering (early defoliation – ED), it can also decrease cluster compactness and regulate yield in high-yielding varieties. This study aimed to evaluate the impact of ED on the physiology and metabolism of Aragonez (syn. Tempranillo) berries along the ripening period. The experiment was set up in 2013 at a commercial vineyard located in the Lisbon winegrowing region.

Read More

Tackling the 3D root system architecture of grapevines: a new phenotyping pipeline based on photogrammetry

Plant roots fulfil important functions as they are responsible for the acquisition of water and nutrients, for anchorage and stability, for interaction with symbionts and, in some cases, for the storage of carbohydrates. These functions are associated with the Root System Architecture (RSA, i.e. the form and the spatial arrangement of the roots in the soil). The RSA results from several biological processes (elongation, ramification, mortality…) genetically determined but with high structural plasticity.

Read More

Effects of progeny in the modulation of the response to water stress in isohydric and anisohydric varieties

Each grapevine variety has a specific water use regulation response under drought, and it is still unclear whether this regulation results from innate genotypic behavior (iso- and anisohydric), or is a response to environmental factors, namely recurrent water stress priming effects. In the present work, we explored the influence of the field-grown genotypes’ drought memory in the drought-response phenotype of their vegetative progenies, in Trincadeira (isohydric) and Castelão (anisohydric) varieties under a drought event followed by recovery in a glasshouse. Cuttings from both cultivars subjected to full irrigation (FI) and non-irrigation (NI) treatments for 5 consecutive years were used.

Read More

Uncovering the interplay between Copper and SO2 tolerance in Saccharomyces cerevisiae

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.20.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...
Read More