OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Analysis and composition of grapes, wines, wine spirits 9 Wine fining with yeast protein extract: effect on polyphenol composition and the related sensorial attributes

Wine fining with yeast protein extract: effect on polyphenol composition and the related sensorial attributes

Abstract

Polyphenols, namely anthocyanins and flavanols, are key compounds for wine color definition and taste perception (astringency and bitterness). During winemaking, several processes could influence the polyphenol composition and, therefore, the organoleptic parameters of wine. 

It is widely known that fining can remove soluble substances, including polymerized tannins and coloring matter in red wines affecting the organoleptic properties of wine. The use of yeast protein extracts (YPE) as fining agents arises from the allergic properties observed in classic protein-based fining agents. From the oenological point of view, it was already verified that this new fining agents promote the clarity and stabilization of wine, however is still a gap in their influence in polyphenol composition and the related organoleptic properties. 

The aim of this work is to understand the molecular mechanisms as how YPE-wine polyphenols interactions could modulate the color as well as the taste sensations (astringency and bitterness) after wine fining with YPE (developed by Proenol, Biotechnology Industry). Briefly, polyphenols related with astringency and bitterness were analyzed by LC-MS. The influence of YPE on wine color was also assayed by CieLab system. Furthermore, the effect of YPE-wine on the interaction with salivary proteins was also analyzed by SDS-PAGE after wine ingestion during a sensory evaluation. Finally, the results from experimental data were compared with the results obtained by sensorial panel. 

Overall, it was concluded that wines clarified with YPE revealed a significant decrease in the majority of identified compounds related to bitterness and astringency. The study of wine color revealed that YPE had the ability to reduce yellow color of white wines and did not remove red color of red and rosé wines, which is an important aspect in consumption market. Besides the decreasing of several polyphenols related with taste perception, some relevant differences were observed in the salivary protein profile by SDS-PAGE. The results observed herein highlighted the relationship between (1) the taste perception, (2) the interaction between salivary proteins and wine polyphenols during the sensorial evaluation and (3) the effect of YPE fining in wine polyphenols. 

In summary, YPE reveals to be a good alternative to protein animal origin fining agents due to the ability to promote wine sensorial properties.

DOI:

Publication date: June 11, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Telmo Francisco, Rosa Pérez, Susana Soares, Nuno Mateus, Victor Freitas, Adriana Xavier, Manuel Figueiredo, Filipe Centeno, Maria Teixeira

LAQV-REQUINTE, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto
PROENOL, Indústria Biotecnológica, Lda, Travessa das Lages nº267, Apto 547, Canelas, VNG 4405-194 Portugal

Contact the author

Keywords

fining, yeast protein extract, organoleptic properties, salivary proteins 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

20-Year-Old data set: scion x rootstock x climate, relationships. Effects on phenology and sugar dynamics

Global warming is one of the biggest environmental, social, and economic threats. In the Douro Valley, change to the climate are expected in the coming years, namely an increase in average temperature and a decrease in annual precipitation. Since vine cultivation is extremely vulnerable and influenced by the climate, these changes are likely to have negative effects on the production and quality of wine.
Adaptation is a major challenge facing the viticulture sector where the choice of plant material plays an important role, particularly the rootstock as it is a driver for adaptation with a wide range of effects, the most important being phylloxera, nematode and salt, tolerance to drought and a complex set of interactions in the grafted plant.
In an experimental vineyard, established in the Douro Region in 1997, with four randomized blocs, with five varieties, Touriga Nacional, Tinta Barroca, Touriga Franca and Tinta Roriz, grafted in four rootstocks, Rupestris du Lot, R110, 196-17C, R99 and 1103P, data was collected consecutively over 20 years (2001-2020). Phenological observations were made two to three times a week, following established criteria, to determine the average dates of budbreak, flowering and veraison. During maturation, weekly berry samples were taken to study the dynamics of sugar accumulation, amongst other parameters. Climate data was collected from a weather station located near the vineyard parcel, with data classified through several climatic indices.
The results achieved show a very low coefficient of variations in the average date of the phenophases and an important contribution from the rootstock in the dynamic of the phenology, allowing a delay in the cycle of up to10-12 days for the different combinations. The Principal Component Analysis performed, evaluating trends in the physical-chemical parameters, highlighted the effect of the climate and rootstock on fruit quality by grape varieties.

Climate modeling at local scale in the Waipara winegrowing region in the climate change context

In viticulture, a warming climate can have a very significant impact on grapevine development and therefore on the quality and characteristics of wines across different spatial scales, ranging from global to local. In order to adapt wine-growing to climate change, global climate models can be used to define future scenarios, but only at the scale of major wine regions. Despite the huge progress made over the last ten years in terms of the spatial resolution of climate models (now downscaled to a few square kilometres), they are not yet sufficiently precise to account for the local climate variability associated with such parameters as local topography, in spite of these parameters being decisive for vine and wine characteristics. This study describes a method to downscale future climate scenarios to vineyard scale. Networks of data loggers have been used to collect air temperature at canopy level in the Waipara winegrowing region (New Zealand) over five growing seasons. These measurements allow the creation of fine-scale geostatistical models and maps of temperature (at 100 m resolution) for the growing season. In order to model climate change at pilot site scale, these geostatistical models have been combined with regional climate change predictions for the periods 2031-2050 and 2081-2100 based on the RCP8.5 climate change scenario. The integration of local climate variability with regionalized climate change simulations allows assessment of the impacts of climate change at the vineyard scale. The improved knowledge gained using this methodology results from the increased horizontal resolution that better addresses the concerns of winegrowers. The results provide the local winegrowers with information necessary to understand current processes, as well as historical and future viticulture trends at the scale of their site, thereby facilitating decisions about future response strategies.

Soil quality in Beaujolais vineyard. Importance of pedology and cultural practices

A pedological study was carried out from 2009 to 2017 in Beaujolais vineyard, to improve physical and chemical knowledge of soils. It was completed in 2016 and 2017 by the current study, dealing with microbial aspects, in order to build a reference frame for improved advice in soil management. Microbial biomass was measured on representative plots of the six most common soil types identified in Beaujolais and, for each soil type, on plots with different levels of the main impacting parameters: total organic carbon, pH, cation exchange capacity, extractable copper. A total of 59 soil samples were collected. Confirming the results of various trials carried out in Beaujolais over the past 20 years, the results of the present study showed that the soils were still alive, but exhibited a large variability of biological parameters, which appeared dependant on both pedological and anthropic factors. Therefore, a good interpretation of biological parameters and advice for vine growers must rely on a pedologically-based referential with differentiated main driving factors. For example, the control of pH is of primary importance in granitic soils and in no way organic matter addition can improve soil quality if pH is too low. Conversely, in calcareous soils, biological parameters are more directly affected by direct or indirect (cover crops for example) inputs of organic matter. The use of biological parameters, such as microbial biomass, is of great potential value to improve advice on agro-viticultural practices (soil management, fertilization, liming, etc.), basis of a sustainable wine production on fragile soils.

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares