terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 The 1000 grapevine genomes project: Cataloguing Australia’s grapevine germplasm

The 1000 grapevine genomes project: Cataloguing Australia’s grapevine germplasm

Abstract

Grapevine cultivars can be unequivocally typed by both physical differences (ampelography) and genetic tests. However due to their very similar characteristics, the identification of clones within a cultivar relies on the accurate tracing of supply records to the point of origin. Such records are not always available or reliable, particularly for older accessions. Whole genome sequencing (WGS) provides the most highly detailed methodology for defining grapevine cultivars and more importantly, this can be extended to differentiating clones within those cultivars.

 

The AWRI has developed a world-first clonal sequencing methodology that combines the latest next-generation genome sequencing technologies, high-performance computing and customised bioinformatics tools. This technique has been successfully used to define clonal variation across 1000 accessions of 20 different cultivars obtained from nurseries and vineyards throughout Australia.

 

To aid in the phylogenetic analysis and identification of intra-cultivar somatic mutations, long-read reference genomes were produced for several cultivars, including Shiraz, Grenache and Sauvignon Blanc. These reference genomes were also used to detect unique structural variations that may be important drivers of the phenotypic differences observed between these cultivars.

Acknowledgements: This work was supported by Wine Australia, with levies from Australia’s grapegrowers and winemakers and matching funds from the Australian Government. Support for DNA sequencing was provided by Bioplatforms Australia as part of the National Collaborative Research Infrastructure Strategy, an initiative of the Australian Government. The AWRI is a member of the Wine Innovation Cluster (WIC) in Adelaide.

DOI:

Publication date: October 4, 2023

Issue: ICGWS 2023

Type: Article

Authors

Cristobal Onetto1*, Christopher Ward1, Steven Van Den Heuvel1, Simon Schmidt1, Anthony Borneman1

1The Australian Wine Research Institute, Glen Osmond, South Australia, Australia

Contact the author*

Keywords

grapevine, germplasm, clonal identification, whole genome sequencing

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

How are canned wine drinkers perceived? An investigation involving Swiss nationals and different scenarios of outdoor leisure activities

This study examines how people who consume wine in cans are perceived in terms of their basic personality characteristics, helps understand the role of cultural background on people’s perception, and verify the role played by the consumption context on the perception. Our hypothesis is that prejudice and negative attitudes towards wine in cans might exert a negative effect on the evaluation of people who consume canned wine. To evaluate this hypothesis, the consumption of wine in cans was evoked in four different contexts of use during outdoor leisure activity (beach resort, ski resort, desert safari, and party). In order to examine the effect of culture on subject’s response we use participants from Switzerland, a country where three different cultures, associated with three different languages, cohabit.

Can yeast cells sense other yeasts beyond competition interactions?

The utilization of non-Saccharomyces yeasts in the wine industry has increased significantly in recent years. Alternative species need commonly be employed in combination with Saccharomyces cerevisiae to avoid stuck fermentation, or microbial spoilage. The employment of more than one yeast starter can lead to interactions between different species with an impact on the outcome of wine fermentation. Previous studies[1] demonstrated that S. cerevisiae elicits transcriptional responses with both shared and species-specific features in co-culture with other yeast species.

Characterization of a Sémillon clonal population: exploring genetic diversity, metabolomic profiles, and phenotypic variations

Sémillon is a cultivated grape variety known for contributing to dry and sweet white wine production. However, only seven approved clones have been officially recognized in France[1]. In this study, we aimed to characterize the genetic diversity and metabolomic profiles of a Sémillon clonal population, shedding light on the potential variations within this important grape variety.

Defoliation combined with exogenous ABA application results in slower ripening and improved anthocyanin profile

Reducing sugar accumulation in grape (Vitis vinifera L.) berries may be a way to mitigate the effect of climate change. Managing canopy and crop load is an effective way to do so, however, reducing canopy size has been demonstrated to induce undesirable effects on anthocyanins. The aim of this study was to test if an application of exogenous ABA on the grape berries of defoliated vines (⅔ of the leaves removed) can result in slower sugar accumulation while maintaining grape and wine quality. An experiment with defoliation and exogenous ABA application on directly on clusters (factorial design 2×2) was performed with ‘Tempranillo’ fruit-bearing cuttings.

Preliminary study of extraction of polysaccharides from pomace by high powered ultrasonic combined with enzymes

Red grape pomace can be an important source of polysaccharides, but currently they are little studied and even less with viable and environmental extraction processes (green extraction). These green techniques must be able to break the cell wall so that the compounds contained in the cells, including polysaccharides, are released and can have a great influence on extraction yields, the chemical structure of polysaccharides and applications in wines. Amongst the emerging green techniques most applied to the extraction of bioactive compounds, such as polysaccharides, high-power ultrasound (US) and enzyme-assisted extraction stand out.