terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 The 1000 grapevine genomes project: Cataloguing Australia’s grapevine germplasm

The 1000 grapevine genomes project: Cataloguing Australia’s grapevine germplasm

Abstract

Grapevine cultivars can be unequivocally typed by both physical differences (ampelography) and genetic tests. However due to their very similar characteristics, the identification of clones within a cultivar relies on the accurate tracing of supply records to the point of origin. Such records are not always available or reliable, particularly for older accessions. Whole genome sequencing (WGS) provides the most highly detailed methodology for defining grapevine cultivars and more importantly, this can be extended to differentiating clones within those cultivars.

 

The AWRI has developed a world-first clonal sequencing methodology that combines the latest next-generation genome sequencing technologies, high-performance computing and customised bioinformatics tools. This technique has been successfully used to define clonal variation across 1000 accessions of 20 different cultivars obtained from nurseries and vineyards throughout Australia.

 

To aid in the phylogenetic analysis and identification of intra-cultivar somatic mutations, long-read reference genomes were produced for several cultivars, including Shiraz, Grenache and Sauvignon Blanc. These reference genomes were also used to detect unique structural variations that may be important drivers of the phenotypic differences observed between these cultivars.

Acknowledgements: This work was supported by Wine Australia, with levies from Australia’s grapegrowers and winemakers and matching funds from the Australian Government. Support for DNA sequencing was provided by Bioplatforms Australia as part of the National Collaborative Research Infrastructure Strategy, an initiative of the Australian Government. The AWRI is a member of the Wine Innovation Cluster (WIC) in Adelaide.

DOI:

Publication date: October 4, 2023

Issue: ICGWS 2023

Type: Article

Authors

Cristobal Onetto1*, Christopher Ward1, Steven Van Den Heuvel1, Simon Schmidt1, Anthony Borneman1

1The Australian Wine Research Institute, Glen Osmond, South Australia, Australia

Contact the author*

Keywords

grapevine, germplasm, clonal identification, whole genome sequencing

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Quantification of polysaccharides of variety Pomaces of the D.O.Ca Rioja

Pomace is one of the main residues generated by the wine industry and represents an environmental problem. Currently, there is a growing interest in the revaluation of these products because different bioactive compounds can be obtained from them, such as polyphenols, grape seed oils and polysaccharides. Red grape pomace can be an important source of polysaccharides, but they are currently little studied and even less with viable and environmental extraction processes (green extraction), such as flash extraction. The residual amount of the fraction rich in pectin (residual pulp) and component rich in hemicellulose in the pomace and the strength of association of the pectin with the cellulose-xyloglucan network depend on the degree of extractability of the polysaccharides in red winemaking and on the winemaking conditions.

Understanding the impact of rising temperatures due to climate change on aromatic compositions in Malbec wines from Mendoza, Argentina

Mendoza is one of Argentina’s most important and outstanding wine regions producing the renowned Malbec wines due to its optimal soil and weather conditions. However, the effects of 21st-century climate change would negatively impact Malbec wines quality. This study investigated the effect of temperature increase and the impact of plant hormone abscisic acid (ABA) used to mitigate the negative effect of temperature increase on Malbec wines aromatic composition through GC-MS. Four treatments were applied on vines at field condition: Control, Control + 3 ºC, ABA and ABA + 3 ºC.

Phenolic extraction and dissolved oxygen concentration during red wines fermentations with Airmixig M.I.™

During red wine fermentation, the extraction of phenolics compounds and sufficient oxygen provision are critical for wine quality [1,2]. In this trial, we aimed at evaluating the kinetics of phenolic extraction and dissolved oxygen during red wine fermentations using the airmixing system. Twenty lots of red grape musts were fermented in 300.000 L tanks, equipped with airmixing, using two injection regimes (i.e., high and low intensity, and high and low daily frequency). An oxygen analyzer was introduced into the tanks in order to record the concentration of dissolved oxygen over time.

Effect of soil particle size on vine water status, leaf ABA content and berry quality in Nebbiolo grapes

The root and shoot abscisic acid (ABA) accumulation in response to water deficit and its relation with stomatal conductance is longtime known in grapevine. ABA-dependent and ABA-independent signalling response to osmotic stress coexist in sessile plants. In grapevine, the signaling role of ABA in response to water stress conditions and its influence on berry quality is critical to manage grapevine acclimation to climate change.

Use of UHPH to improve the implantation of non-Saccharomyces yeasts

Ultra High-Pressure Homogenization (UHPH) is a high-pressure pumping at 300 MPa (>200 MPa) with a subsequent depressurization against a highly resistant valve made of tungsten carbide covered by ceramic materials or carbon nanoparticles. The intense impact and shear efforts produce the nano-fragmentation of colloidal biopolymers including the elimination of microorganism (pasteurization or sterilization depending on in-valve temperature) and the inactivation of enzymes.