terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Identification of a stable epi-allele associated with flower development and low bunch compactness in a somatic variant of Tempranillo Tinto

Identification of a stable epi-allele associated with flower development and low bunch compactness in a somatic variant of Tempranillo Tinto

Abstract

Grapevine cultivars are vegetatively propagated to preserve their varietal characteristics. However, spontaneous somatic variations that occur and are maintained during cycles of vegetative growth offer opportunities for the natural improvement of traditional grape cultivars. One advantageous trait for winegrowing is reduced bunch compactness, which decreases the susceptibility to pests and fungal diseases and favor an even berry ripening. In this study, we examined a loose bunch somatic variant of Tempranillo Tinto cultivar (TT) to elucidate the molecular basis underlying this variation. The variant displayed a masculinized flower phenotype, characterized by an underdeveloped yet functional gynoecium, with reduced development of the style, stigma and septum, as compared to the complete hermaphroditic flowers typically observed in TT. Genetic analysis of its self-progeny demonstrated the co-segregation of the masculinized flower phenotype with the hermaphrodite allele of the grape sex locus (SDR)[1]. While genome re-sequencing did not identify any genetic variation within the SDR locus, an RNA-seq analysis identified one SDR-located gene over-expressed in the somatic variant compared to a control TT clone. The hermaphrodite allele was specifically over-expressed in the variant. Both Illumina WGBS and Nanopore epigenomic analyses identified a hermaphrodite allele-specific hyper-methylated region upstream of the upregulated gene. Analysis of chromatin conformation capture (3C) revealed a three-dimensional rearrangement of the locus, including the presence of a novel 36 kb chromatin loop delimiting the hyper-methylated region, which could be responsible for the over-expression and the phenotype. These findings indicate that somatic epi-alleles in the SDR locus can determine variation in gynoecium development leading to decreased fruit set and looser bunches in grapevine.

Acknowledgements: This study is part of the projects BIO-2017-86375-R and PID2020-120183RB-I00, and PRE-2018-086017 grant funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. This study is also part of the vWISE project.

1)  Massonnet, M., Cochetel, N., Minio, A., Vondras, A. M., Lin, J., Muyle, A., Garcia, J. F., Zhou, Y., Delledonne, M., Riaz, S., Figueroa-Balderas, R., Gaut, B. S., & Cantu, D. (2020). The genetic basis of sex determination in grapes. Nature Communications, 11(1), 1–12. DOI: 10.1038/s41467-020-16700-z

DOI:

Publication date: October 4, 2023

Issue: ICGWS 2023

Type: Article

Authors

Alañón, Noelia1*; Ferradás, Yolanda2; Lijavetzky, Diego3; Ferrero, Lucía4; Martínez-Zapater, José Miguel1; Ariel, Federico4; Carbonell-Bejerano, Pablo1; Ibáñez, Javier1

1 Instituto de Ciencias de la Vid y del Vino (ICVV, CSIC-CAR-UR), Departamento de Viticultura, Logroño, Spain
2 Departamento de Biología Funcional, Universidad de Santiago de Compostela, Santiago de Compostela, Spain
3 Instituto de Biología Agrícola de Mendoza (IBAM), CONICET, FCA-UNCuyo, Almirante Brown 500, M5528AHB, Chacras de Coria, Mendoza, Argentina
4 Instituto de Agrobiotecnología del Litoral, CONICET, Universidad Nacional del Litoral, Colectora Ruta Nacional 168km 0, 3000, Santa Fe, Argentina

Contact the author*

Keywords

bunch compactness, somatic variation, flower development, chromatin conformation, epiallele

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Metabolomic profiling of botrytized grape berries: unravelling the dynamic chemical transformations during noble rot

Botrytis cinerea, a fungal pathogen commonly known as grey mold, which under specific climatic conditions can develop into a desirable form known as noble rot. In this process the fungus penetrates the grape skin, allowing water evaporation and concentration of sugars and flavors, while profoundly affects the metabolite composition of grapes, leading to the production of unique and desirable compounds in the resulting wines. The result is a unique and complex wine with a luscious sweetness, heightened aromatics, and a distinct character.

Quantification of polysaccharides of variety Pomaces of the D.O.Ca Rioja

Pomace is one of the main residues generated by the wine industry and represents an environmental problem. Currently, there is a growing interest in the revaluation of these products because different bioactive compounds can be obtained from them, such as polyphenols, grape seed oils and polysaccharides. Red grape pomace can be an important source of polysaccharides, but they are currently little studied and even less with viable and environmental extraction processes (green extraction), such as flash extraction. The residual amount of the fraction rich in pectin (residual pulp) and component rich in hemicellulose in the pomace and the strength of association of the pectin with the cellulose-xyloglucan network depend on the degree of extractability of the polysaccharides in red winemaking and on the winemaking conditions.

Unveiling a hidden link: does time hold the key to altered spectral signatures of grapevines under drought?

Remote sensing technology captures spectral data beyond the visible range, making it useful for monitoring plant stress. Vis-NIR (Visible-Near Infrared) spectroscopy (400-1000 nm) is commonly used to indirectly assess plant status during drought. One example is the widespread use of normalized difference vegetation index (NDVI) that is strongly linked to green biomass. However, a knowledge gap exists regarding the applicability of this method to all the drought conditions and if it is a direct correlation to the water status of the plant.

Impact of polyclonal selection for abiotic stress tolerance on the yield and must quality traits of grapevine varieties

The effects of climate change in viticulture are currently a major concern, with heat waves and drought affecting yield, wine quality, and in extreme cases, even plant survival. Ancient grapevine varieties have high intravarietal genetic variability that so far has been explored successfully to improve yield and must quality. Currently, there is little information available on intravarietal variability regarding responses to stress. In the current work, the intravarietal genetic variability of several Portuguese varieties was studied for yield, must quality, and tolerance to abiotic stress, through indirect, rapid, and nondestructive measurements carried out in the field.

Glucosidase and esterase salivary activities and their involvement in consumer’s wine sensory perception and liking

Wine flavour is the integration of distinct physiologically defined sensory systems that combine taste, aroma and trigeminal sensations, and it is a key determinant factor for the acceptance of wine by consumers. Volatile compounds, are important contributors to wine flavour, specially to aroma. These small and low-boiling point compounds are easily released into the air allowing to enter and move within the nasal or oral cavities where they can bind the olfactory receptors. Additionally, wine also contains aroma precursors, which are non-volatile compounds, but that can be broken down releasing volatile odorants. During wine tasting, all these chemicals (volatiles and non-volatiles) can be submitted to the action of salivary enzymes.