terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Bioprotection of grape must by Metschnikowia sp.: genericity and mechanism

Bioprotection of grape must by Metschnikowia sp.: genericity and mechanism

Abstract

The market trend heads to food products with less chemical inputs, including in oenology. During the winemaking process, sulfites are commonly use to avoid microbiological contamination and stabilization of the wine thanks to its antimicrobial and antioxidant activities. Nevertheless, this use is not without consequences on human health and environment, leading for example to allergic reaction and pollution. A biological alternative to these sulfites has emerges: the bioprotection. This method consists to inoculate at the pre-fermentation stage, a microorganism able to inhibit the growth of the negative indigenous flora. The genus Metschnikowia is well know to have this bioprotection property, especially M. pulcherrima and M. fructicola, but the mechanisms remain poorly studied. This project aims to study the bioprotection abilities of 50 strains of Metschnikowia, including 16 species, against Gluconobacter oxydansand Brettanomyces bruxellensis, both known to lead to defects in organoleptic properties of the wine. To investigate the bioprotection effect, grape juice was inoculated with Metschnikowia sp. and G. oxydans or Metschnikowia sp. And B. bruxellensis. The capacity of Metschnikowia to inhibit G. oxydans growth was evaluated for 7 days by plate counting and by digital PCR for B. bruxellensis. In parallel, Metschnikowia species are inoculated in synthetic grape must to quantify the production of the acid pulcherriminic precursor. This acid is considered as a way for the yeast to compete for iron in the medium, and thus as a method of bioprotection. The results show a diversity in the bioprotection effect towards the spoilage microorganisms and in the production of the acid pulcherriminic precursor. The bioprotection could be an alternative in the sustainable pre-fermentative management of winemaking process.

Acknowledgements: We were grateful to Région Occitanie and INRAE MICA department for funding this project.

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Julie Aragno1, Angèle Thiriet2, Pascale Fernandez-Valle1, Cécile Grondin2, Jean-Luc Legras1,2, Carole Camarasa1, Audrey Bloem1

1 UMR SPO, Université Montpellier, INRAE, Institut Agro, Montpellier, France
2 CIRM Levures, UMR SPO, Montpellier

Contact the author*

Keywords

alcoholic fermentation, bioprotection, Metschnikowia sp., diversity

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Predicting provenance and grapevine cultivar implementing machine learning on vineyard soil microbiome data: implications in grapevine breeding

The plant rhizosphere microbial communities are an essential component of plant microbiota, which is crucial for sustaining the production of healthy crops. The main drivers of the composition of such communities are the growing environment and the planted genotype. Recent viticulture studies focus on understanding the effects of these factors on soil microbial composition since microbial biodiversity is an important determinant of plant phenotype, and of wine’s organoleptic properties. Microbial biodiversity of different wine regions, for instance, is an important determinant of wine terroir.

The tolerance of grapevine rootstocks to water deficit is related to root morphology and xylem anatomy traits 

Climate change is altering water balances, thereby compromising water availability for crops. In grapevine, the strategic selection of genotypes more tolerant to soil water deficit can improve the resilience of the vineyard under this scenario. Previous studies demonstrated that root anatomical and morphological traits determine vine performance under water deficit conditions. Therefore, 13 ungrafted rootstock genotypes, 6 commercial (420 A, 41 B, Evex 13-5, Fercal, 140 Ru y 110 R), and 7 from new breeding programs (RG2, RG3, RG4, RG7, RG8, RG9 and RM2) were evaluated in pots during 2021 and 2022.

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

Teinturier grapes: Valorization as a source of high-value compounds for the Chilean food industry

The agri-food industry is constantly searching for ingredients of high functional value, healthy and of natural origin. One species of particular interest is Vitis vinifera, due to its recognized antioxidant potential. Among the grape varieties, one group possesses these antioxidant compounds not only in the skin, but also in its pulp: Teinturier. The red grape has traditionally been used for color correction purposes in winemaking, however, its high antioxidant content transforms it into a raw material of high potential for new formulations of ingredients and foods for the health and wellness market.

New tool to evaluate color modifications during oxygen consumption in white and red wines

Measuring the effect of oxygen consumption on the color of wines as the level of dissolved oxygen decreases over time is very useful to know how much oxygen a wine can consume without significantly altering its color. The changes produced in wine after being exposed to high oxygen concentrations have been studied by different authors, but in all cases the wine has been analyzed once the oxygen consumption process has been completed. This work presents the results obtained with the use of an equipment designed and made to measure simultaneously the level of dissolved oxygen and the spectrum of the wine, during the oxygen consumption process from saturation levels with air to very low levels, which indicate the total consumption of the dosed oxygen[1,2].