terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Bioprotection of grape must by Metschnikowia sp.: genericity and mechanism

Bioprotection of grape must by Metschnikowia sp.: genericity and mechanism

Abstract

The market trend heads to food products with less chemical inputs, including in oenology. During the winemaking process, sulfites are commonly use to avoid microbiological contamination and stabilization of the wine thanks to its antimicrobial and antioxidant activities. Nevertheless, this use is not without consequences on human health and environment, leading for example to allergic reaction and pollution. A biological alternative to these sulfites has emerges: the bioprotection. This method consists to inoculate at the pre-fermentation stage, a microorganism able to inhibit the growth of the negative indigenous flora. The genus Metschnikowia is well know to have this bioprotection property, especially M. pulcherrima and M. fructicola, but the mechanisms remain poorly studied. This project aims to study the bioprotection abilities of 50 strains of Metschnikowia, including 16 species, against Gluconobacter oxydansand Brettanomyces bruxellensis, both known to lead to defects in organoleptic properties of the wine. To investigate the bioprotection effect, grape juice was inoculated with Metschnikowia sp. and G. oxydans or Metschnikowia sp. And B. bruxellensis. The capacity of Metschnikowia to inhibit G. oxydans growth was evaluated for 7 days by plate counting and by digital PCR for B. bruxellensis. In parallel, Metschnikowia species are inoculated in synthetic grape must to quantify the production of the acid pulcherriminic precursor. This acid is considered as a way for the yeast to compete for iron in the medium, and thus as a method of bioprotection. The results show a diversity in the bioprotection effect towards the spoilage microorganisms and in the production of the acid pulcherriminic precursor. The bioprotection could be an alternative in the sustainable pre-fermentative management of winemaking process.

Acknowledgements: We were grateful to Région Occitanie and INRAE MICA department for funding this project.

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Julie Aragno1, Angèle Thiriet2, Pascale Fernandez-Valle1, Cécile Grondin2, Jean-Luc Legras1,2, Carole Camarasa1, Audrey Bloem1

1 UMR SPO, Université Montpellier, INRAE, Institut Agro, Montpellier, France
2 CIRM Levures, UMR SPO, Montpellier

Contact the author*

Keywords

alcoholic fermentation, bioprotection, Metschnikowia sp., diversity

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effect of irrigation in cover cropping vineyards

Cover cropping in vineyard is a sustainable and alternative soil management system to conventional tillage that is gaining more and more importance among winegrowers and is being promoted, among other organizations, by the European Union through the eco-schemes of the Common Agricultural Policy. However, the use of cover crops in Mediterranean viticultural environments is conditioned, to a large extent, by the availability of irrigation water which, in a context of global warming like the one we are experiencing, must be adjusted to savings strategies, supplying to the vine only what it needs in each moment.

The generation of suspended cell wall material may limit the effect of ultrasound in some varieties

The disruptive effect exerted by high-power ultrasound (US) on plant cell walls, natural barriers to the diffusion of compounds of interest during the maceration of red wines, is established as the reason behind the chromatic improvement that its treatment causes. However, sometimes this improvement is not observed, especially with short maceration times. The presence of a high quantity of suspended cell wall material, which formation is favored by the sonication, could be the cause of this lack of positive results since this cell wall material has a high affinity for phenolic compounds.

Moderate wine consumption – part of a balanced diet or a health risk?

Consumption of wine/alcoholic beverages remains a topic of great uncertainty and controversy worldwide. The term “no safe level” dominates the media communication and policy ever since population studies in 2018 [1,2] were published, which denied the existence of a J-curve and suggested that ANY consumption of an alcoholic beverage is harmful to health. The scientific evidence accumulated during the past decades about the health benefits of moderate wine consumption, were questioned and drinking guidelines considered to be too loose.

White grape must processed by UHPH as an alternative to SO2 addition: Effect on the phenolic composition in three varieties

The quantity and distribution of polyphenols in musts play a fundamental role in the white winemaking. This is because these substances are exposed to oxidation reactions, which are catalysed by the polyphenol oxidase (PPO), leading to a decrease in the quality of the wines produced. PPO is inactivated by SO2, but currently, due to the restrictions of the legislation, other methodologies are being investigated. Ultra-High Pressure Homogenization (UHPH) is a non-thermal physic technology that exerts an ultrahigh pressure pumping (>200 MPa) of a fluid through a valve in a continuous system.

Prediction of aromatic attributes of red wines from its colour properties 

Wine perception is a multisensory experience that makes use of the sight, smell, and taste senses. When wine is sensorially assessed, the stimulus received generates multiple signals that tasters convert into organoleptic descriptors. Colour is commonly the first attribute evaluated during wine tasting. Moreover, the colour properties provide the taster with a priori information of the wine’s aroma. This preconceived perception is later confirmed or denied during the aroma evaluation.