terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic study of wild grapevines in La Rioja region

Genetic study of wild grapevines in La Rioja region

Abstract

Since the mid-1980s, several surveys have been carried out in La Rioja to search for populations of the sylvestrisgrapevine subspecies (Vitis vinifera L. subsp. sylvestris Gmelin). The banks of the Ebro River and its tributaries (Alhama, Cidacos, Leza, Iregua, Najerilla, Oja and Tirón rivers), as well as the surrounding vegetation of their valleys have been covered. So far, all the populations found are alluvial, forming part of the riparian vegetation of the Najerilla (the first reported population in La Rioja [1]), Iregua, and the vicinity of Oja valleys. Nevertheless, doubts arose from the beginning about the ‘sylvestris purity’ of some of these wild vines. In this work, leaves and/or cuttings from 83 vines have been used for genetic analyses: 63 vines were sampled in the wild in La Rioja region (17 from Najerilla banks, 43 from Iregua banks, and 3 from Agualinos stream, tributary of the Oja river), and 20 vines were sampled in the grapevine sylvestris collection of the University of La Rioja, originally collected as wild vines from the Najerilla valley. DNAs were extracted and genotyped at 6 microsatellite and 240 SNP markers. A total of 58 different genotypes were found, 20 in the Najerilla population, 36 in the Iregua population and 2 in the Oja population. These genotypes were included in a large genetic structure analysis of more than 2.800 genotypes representing worldwide sylvestrisand sativa subspecies. More than 84% of the wild samples from La Rioja showed a high membership coefficient to the sylvestris population, supporting their sylvestris condition. A local analysis focused on genotypes of cultivated and wild vines found in La Rioja separated them into two main clusters, with a clear division between sylvestris and cultivated vines. Some wild vine genotypes were found in intermediate positions between these two groups, indicating the existence of an introgression of the sativa gene pool into the sylvestris population, constituting an additional threat to the sylvestris subspecies.

Acknowledgements: This work is part of the project “Diversidad genética en la vid y adaptación al cambio climático” (PID2020-120183RB-I00), funded by MCIN / AEI /10.13039/501100011033.

1)  De Toda F. M. and J. C. Sancha (1999) Characterization of Wild Vines in La Rioja (Spain). Am. J. Enol. Vitic. 50: 443-446, doi: 10.5344/ajev.1999.50.4.443.

DOI:

Publication date: October 9, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Javier Ibáñez1*, Javier Tello1, Fernando Martínez de Toda1, José Manuel Valle2, Álvaro Rodríguez-Miranda2, Carlos Alvar Ocete3, José Miguel Martínez-Zapater1, Rafael Ocete3

1 Instituto de Ciencias de la Vid y del Vino (CSIC, UR, CAR). Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño, La Rioja. Spain
2 Built Heritage Research Group (GPAC), University of the Basque Country (UPV/EHU), Vitoria- Gasteiz. Spain
3 Freelance, Tirgo, La Rioja. Spain

Contact the author*

Keywords

introgression, genetic structure, microsatellite, SNP, sylvestris, Vitis vinifera, wild

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Evaluation of the effects of pruning methodology on the development of young vines 

Grapevine pruning is one of the most important practices in the vineyards. Winegrowers use it to provide the vines the shape needed, or to maintain it once achieved, and also to balance vegetative growth and fruit production. In the last decades, careless pruning has been blamed, among other factors, as responsible of the vineyard decay that is been observed even in young vines. However, to our knowledge, there is a lack of systematic research trying to elucidate to which extent the pruning method used affects plant development or its susceptibility to grapevine trunk diseases (GTD). Within this context, the aim of this work is to study the influence of different pruning method strategies on the development of field-planted young vines.

Combined use of leaf removal and natural shading to delay grape ripening in Manto negro (Vitis vinifera L.) under deficit irrigation 

The increasingly frequent heat waves during grape ripening pose challenges for premium wine grape production. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 and 2022 using Manto negro wine grapes to study the effect of two irrigation strategies and different light exposure levels on grape quality.

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

Genetic identification of 200-year-old Serbian grapevine herbarium

Botanist Andreas Raphael Wolny collected a grapevine herbarium from 1812-1824 in Sremski Karlovci (wine region of Vojvodina, Serbia), which represents local cultivated grapevine diversity before the introduction of grape phylloxera in the region. The herbarium comprises over 100 samples organized into two subcollections based on berry colour (red and white varieties), totaling 47 different grape varieties. The objective of this study was to investigate the historical varietal assortment of Balkan and Pannonian winegrowing areas with long viticulture traditions.

Impact of temperature and solar radiation on grape composition variability in the Saint-Emilion winegrowing area 

Grape composition is strongly influenced by climate conditions. Their expected modifications in near future, notably because of increased temperatures, could significantly modify the biochemical composition of berries at harvest, and thus wine typicity and quality. Elevated temperatures favor sugar accumulation in grapes, enhance malic acid degradation and modify the amino acid content. They also reduce significantly anthocyanin accumulation in Merlot, leading to the imbalance between anthocyanins and sugars, while no significant effects on final anthocyanin levels were reported in Tempranillo[1] and finally affect aromas or aroma precursors.