terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic study of wild grapevines in La Rioja region

Genetic study of wild grapevines in La Rioja region

Abstract

Since the mid-1980s, several surveys have been carried out in La Rioja to search for populations of the sylvestrisgrapevine subspecies (Vitis vinifera L. subsp. sylvestris Gmelin). The banks of the Ebro River and its tributaries (Alhama, Cidacos, Leza, Iregua, Najerilla, Oja and Tirón rivers), as well as the surrounding vegetation of their valleys have been covered. So far, all the populations found are alluvial, forming part of the riparian vegetation of the Najerilla (the first reported population in La Rioja [1]), Iregua, and the vicinity of Oja valleys. Nevertheless, doubts arose from the beginning about the ‘sylvestris purity’ of some of these wild vines. In this work, leaves and/or cuttings from 83 vines have been used for genetic analyses: 63 vines were sampled in the wild in La Rioja region (17 from Najerilla banks, 43 from Iregua banks, and 3 from Agualinos stream, tributary of the Oja river), and 20 vines were sampled in the grapevine sylvestris collection of the University of La Rioja, originally collected as wild vines from the Najerilla valley. DNAs were extracted and genotyped at 6 microsatellite and 240 SNP markers. A total of 58 different genotypes were found, 20 in the Najerilla population, 36 in the Iregua population and 2 in the Oja population. These genotypes were included in a large genetic structure analysis of more than 2.800 genotypes representing worldwide sylvestrisand sativa subspecies. More than 84% of the wild samples from La Rioja showed a high membership coefficient to the sylvestris population, supporting their sylvestris condition. A local analysis focused on genotypes of cultivated and wild vines found in La Rioja separated them into two main clusters, with a clear division between sylvestris and cultivated vines. Some wild vine genotypes were found in intermediate positions between these two groups, indicating the existence of an introgression of the sativa gene pool into the sylvestris population, constituting an additional threat to the sylvestris subspecies.

Acknowledgements: This work is part of the project “Diversidad genética en la vid y adaptación al cambio climático” (PID2020-120183RB-I00), funded by MCIN / AEI /10.13039/501100011033.

1)  De Toda F. M. and J. C. Sancha (1999) Characterization of Wild Vines in La Rioja (Spain). Am. J. Enol. Vitic. 50: 443-446, doi: 10.5344/ajev.1999.50.4.443.

DOI:

Publication date: October 9, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Javier Ibáñez1*, Javier Tello1, Fernando Martínez de Toda1, José Manuel Valle2, Álvaro Rodríguez-Miranda2, Carlos Alvar Ocete3, José Miguel Martínez-Zapater1, Rafael Ocete3

1 Instituto de Ciencias de la Vid y del Vino (CSIC, UR, CAR). Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño, La Rioja. Spain
2 Built Heritage Research Group (GPAC), University of the Basque Country (UPV/EHU), Vitoria- Gasteiz. Spain
3 Freelance, Tirgo, La Rioja. Spain

Contact the author*

Keywords

introgression, genetic structure, microsatellite, SNP, sylvestris, Vitis vinifera, wild

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Grapevine cultivation is threatened by the global warming, which combines high temperatures and reduced rainfall, impacting in wine quality and even plant survival. Breeding for varieties resilient to these challenges must address plant traits such as tolerance to supraoptimal temperatures and optimized water use efficiency while minimizing productivity and quality losses. Stomatal abundance (SA) determines the maximum leaf potential for transpiration and thus water loss and cooling. Since SA results from a developmental process during leaf emergence and growth, knowledge on the genetic control of this process would provide specific targets for modification.

Bioprotection of grape must by Metschnikowia sp.: genericity and mechanism

The market trend heads to food products with less chemical inputs, including in oenology. During the winemaking process, sulfites are commonly use to avoid microbiological contamination and stabilization of the wine thanks to its antimicrobial and antioxidant activities. Nevertheless, this use is not without consequences on human health and environment, leading for example to allergic reaction and pollution. A biological alternative to these sulfites has emerges: the bioprotection.

Advancing grapevine science through genomic research

The seminar will examine the complexities and prospects of genomic research on Vitis species, characterize by exceptionally high heterozygosity and common interspecific gene flow. The seminar will showcase case studies highlighting the critical role of diploid genome references in grape research, specifically in areas such as aroma development, disease resistance, and domestication traits. It will also address the emerging focus on pangenomes within the Vitis genus, particularly in the context of genetic studies on naturally interbreeding populations.

Entomopathogenic nematodes application for controlling Lobesia botrana in grapevine and their impact on grapevine quality 

Entomopathogenic nematodes (EPN) are well-known biological control agents combined with specific adjuvants that now allow their use against aerial pests. Lobesia botrana (Lepidoptera: Tortricidae) is one of the major harmful pests detected in worldwide vineyards. Previous studies demonstrated that the EPNs Steinernema feltiae and S. carpocapsae could control L. botrana. The hypothesis was that the best combination of EPN-adjuvant/timing (season/temperatures) will support the use of EPN in the vineyard against L. botrana with no impact on the grape performance.

Effect of ultraviolet B radiation on pathogenic molds of grapes

The fungicidal effect of UV-C radiation (100-280 nm wavelength) is well known, but its applicability for the control of pathogenic molds of grapes is conditioned by its effect on the host and by the risks inherent in its handling[1].
As an alternative, the effect in vitro of UV-B radiation (280-315 nm) on the main pathogenic molds of grapes has been studied: Botrytis cinerea, Aspergillus niger, Penicillium expansum and Rhizopus stolonifer.