terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic study of wild grapevines in La Rioja region

Genetic study of wild grapevines in La Rioja region

Abstract

Since the mid-1980s, several surveys have been carried out in La Rioja to search for populations of the sylvestrisgrapevine subspecies (Vitis vinifera L. subsp. sylvestris Gmelin). The banks of the Ebro River and its tributaries (Alhama, Cidacos, Leza, Iregua, Najerilla, Oja and Tirón rivers), as well as the surrounding vegetation of their valleys have been covered. So far, all the populations found are alluvial, forming part of the riparian vegetation of the Najerilla (the first reported population in La Rioja [1]), Iregua, and the vicinity of Oja valleys. Nevertheless, doubts arose from the beginning about the ‘sylvestris purity’ of some of these wild vines. In this work, leaves and/or cuttings from 83 vines have been used for genetic analyses: 63 vines were sampled in the wild in La Rioja region (17 from Najerilla banks, 43 from Iregua banks, and 3 from Agualinos stream, tributary of the Oja river), and 20 vines were sampled in the grapevine sylvestris collection of the University of La Rioja, originally collected as wild vines from the Najerilla valley. DNAs were extracted and genotyped at 6 microsatellite and 240 SNP markers. A total of 58 different genotypes were found, 20 in the Najerilla population, 36 in the Iregua population and 2 in the Oja population. These genotypes were included in a large genetic structure analysis of more than 2.800 genotypes representing worldwide sylvestrisand sativa subspecies. More than 84% of the wild samples from La Rioja showed a high membership coefficient to the sylvestris population, supporting their sylvestris condition. A local analysis focused on genotypes of cultivated and wild vines found in La Rioja separated them into two main clusters, with a clear division between sylvestris and cultivated vines. Some wild vine genotypes were found in intermediate positions between these two groups, indicating the existence of an introgression of the sativa gene pool into the sylvestris population, constituting an additional threat to the sylvestris subspecies.

Acknowledgements: This work is part of the project “Diversidad genética en la vid y adaptación al cambio climático” (PID2020-120183RB-I00), funded by MCIN / AEI /10.13039/501100011033.

1)  De Toda F. M. and J. C. Sancha (1999) Characterization of Wild Vines in La Rioja (Spain). Am. J. Enol. Vitic. 50: 443-446, doi: 10.5344/ajev.1999.50.4.443.

DOI:

Publication date: October 9, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Javier Ibáñez1*, Javier Tello1, Fernando Martínez de Toda1, José Manuel Valle2, Álvaro Rodríguez-Miranda2, Carlos Alvar Ocete3, José Miguel Martínez-Zapater1, Rafael Ocete3

1 Instituto de Ciencias de la Vid y del Vino (CSIC, UR, CAR). Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño, La Rioja. Spain
2 Built Heritage Research Group (GPAC), University of the Basque Country (UPV/EHU), Vitoria- Gasteiz. Spain
3 Freelance, Tirgo, La Rioja. Spain

Contact the author*

Keywords

introgression, genetic structure, microsatellite, SNP, sylvestris, Vitis vinifera, wild

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Crown procyanidin quantification in red wines, rosé wines and Port wines

Condensed grape tannins play a major role in the organoleptic properties and quality of red wine. Recently, a new sub-family of macrocyclic condensed tannins has been identified in red wine and named “crown tannins”. Indeed, the first compound of the family identified and characterised by NMR was the crown procyanidin tetramer which is composed of a macrocyclic structure composed of four (-)-epicatechins link together by B-type interflavanoid linkage in the following an alternative sequences of C4-C8 and C4-C6 linkage. The 3D structure of this unusual crown procyanidin family reveals a central cavity in the molecule [1].

Inert gases persistence in wine storage tank blanketing

It is common to find tanks in the winery with wine below their capacity due to wine transfers between tanks of different capacities or the interruption of operations for periods of a few days. This situation implies the existence of an ullage space in the tank with prolonged contact with the wine causing its absorption/oxidation. Oxygen uptake from the air headspace over the wine due to differences in the partial pressure of O2 can be rapid, up to 1.5 mL of O2 per liter of wine in one hour and 100 cm2 of surface area1 and up to saturation after 4 hours.

Impact of toasting and botanical origin on oak wood (Q. sp.) volatilome using untargeted GCxGC-ToFMS analysis

Many works have been carried out to identify the key aroma volatile compounds of oak wood (e.g., whisky-lactone, furfural, maltol, eugenol, guaiacol, vanillin) using conventional gas chromatography coupled with olfactometry and mass spectrometry (GC-O-MS). Inspired by recent untargeted approaches in the field of food “omics”, this work aims to extend our knowledge on the impact of cooperage process on the volatile composition of oak wood using two-dimensional comprehensive gas chromatography coupled with time of flight mass spectrometry (GCxGC-ToFMS).

Selecting green cover species in the under-trellis zone of Lower Austrian vineyards

The under-trellis zone of vineyards is a sensitive area through which vines cover a significant portion of their nutrient and water needs. Mechanical and chemical methods are applied to suppress competing and tall-growing weeds to ensure optimal vine growth conditions. In addition to higher operating costs and depending on the soil conditions, these practices might lead to a long-term reduction in soil fertility and biodiversity. The presented study aims to analyse the suitability and interspecies competition of a selected green cover mixture of five local herbaceous species as potential green cover mixture in the under-trellis area of Lower Austrian vineyards.

Exploring the prevalence of esca-induced leaf symptoms in French vineyards and the role of climate: a national scale analysis

Esca, a severe trunk disease affecting vineyards, is caused by fungal pathogens that induce wood necrosis and decay, leaf symptoms, yield losses, and potentially a rapid death of the vine. The prevalence of this disease varies across years, regions, cultivars, and plot ages. Despite its significance in understanding and predicting dieback risk in different vineyards, the role of climate in trunk diseases remains a relatively unexplored research area. While some studies have demonstrated the impact of certain climatic conditions on the prevalence of the disease, they often focus on a limited number of plots and yield conflicting results.We conducted a statistical analysis, using a Bayesian approach on a national database comprising prevalence data of esca from over 500 different plots in France, spanning the years 2003 to 2022 and encompassing various cultivars.