terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic study of wild grapevines in La Rioja region

Genetic study of wild grapevines in La Rioja region

Abstract

Since the mid-1980s, several surveys have been carried out in La Rioja to search for populations of the sylvestrisgrapevine subspecies (Vitis vinifera L. subsp. sylvestris Gmelin). The banks of the Ebro River and its tributaries (Alhama, Cidacos, Leza, Iregua, Najerilla, Oja and Tirón rivers), as well as the surrounding vegetation of their valleys have been covered. So far, all the populations found are alluvial, forming part of the riparian vegetation of the Najerilla (the first reported population in La Rioja [1]), Iregua, and the vicinity of Oja valleys. Nevertheless, doubts arose from the beginning about the ‘sylvestris purity’ of some of these wild vines. In this work, leaves and/or cuttings from 83 vines have been used for genetic analyses: 63 vines were sampled in the wild in La Rioja region (17 from Najerilla banks, 43 from Iregua banks, and 3 from Agualinos stream, tributary of the Oja river), and 20 vines were sampled in the grapevine sylvestris collection of the University of La Rioja, originally collected as wild vines from the Najerilla valley. DNAs were extracted and genotyped at 6 microsatellite and 240 SNP markers. A total of 58 different genotypes were found, 20 in the Najerilla population, 36 in the Iregua population and 2 in the Oja population. These genotypes were included in a large genetic structure analysis of more than 2.800 genotypes representing worldwide sylvestrisand sativa subspecies. More than 84% of the wild samples from La Rioja showed a high membership coefficient to the sylvestris population, supporting their sylvestris condition. A local analysis focused on genotypes of cultivated and wild vines found in La Rioja separated them into two main clusters, with a clear division between sylvestris and cultivated vines. Some wild vine genotypes were found in intermediate positions between these two groups, indicating the existence of an introgression of the sativa gene pool into the sylvestris population, constituting an additional threat to the sylvestris subspecies.

Acknowledgements: This work is part of the project “Diversidad genética en la vid y adaptación al cambio climático” (PID2020-120183RB-I00), funded by MCIN / AEI /10.13039/501100011033.

1)  De Toda F. M. and J. C. Sancha (1999) Characterization of Wild Vines in La Rioja (Spain). Am. J. Enol. Vitic. 50: 443-446, doi: 10.5344/ajev.1999.50.4.443.

DOI:

Publication date: October 9, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Javier Ibáñez1*, Javier Tello1, Fernando Martínez de Toda1, José Manuel Valle2, Álvaro Rodríguez-Miranda2, Carlos Alvar Ocete3, José Miguel Martínez-Zapater1, Rafael Ocete3

1 Instituto de Ciencias de la Vid y del Vino (CSIC, UR, CAR). Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño, La Rioja. Spain
2 Built Heritage Research Group (GPAC), University of the Basque Country (UPV/EHU), Vitoria- Gasteiz. Spain
3 Freelance, Tirgo, La Rioja. Spain

Contact the author*

Keywords

introgression, genetic structure, microsatellite, SNP, sylvestris, Vitis vinifera, wild

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Induction of polyphenols in seedlings of Vitis vinifera cv. Monastrell by the application of elicitors

Contamination problems arising from the use of pesticides in viticulture have raised concerns. One of the alternatives to reduce contamination is the use of elicitors, molecules capable of stimulating the natural defences of plants, promoting the production of phenolic compounds (PC) that offer protection against biotic and abiotic stress. Previous studies on Cabernet-Sauvignon seedlings demonstrated that foliar application of elicitors methyl jasmonate (MeJ) and benzothiadiazole (BTH) increased proteins and PC involved in grapevine defence mechanisms. However, no trials had been conducted on Monastrell seedlings, a major winegrape variety in Spain.

The use of δ13C as an indicator of water use efficiency for the selection of drought tolerant grapevine varieties

In the context of climate change with increasing evaporative demand, understanding the water use behavior of different grapevine cultivars is of critical importance. Carbon isotope discrimination (δ13C) measurements in wine provide a precise and integrated assessment of the water status of the vines during the sugar accumulation period in grape berries. When collected over multiple vintages on different cultivars, δ13C measurements can also provide insights into the effects of genotype on water use efficiency.

INTEGRAPE guidelines and tools: an effort of COST Action CA17111

INTEGRAPE was a European interdisciplinary network for “data integration to maximize the power of omics for grapevine improvement” (CA17111, https://integrape.eu/), funded by the European COST Association from September 2018 to 2022. This Action successfully developed guidelines and tools for data management and promoted the best practices in grapevine omics studies with a holistic future vision of: “Imagine having all data on grapevine accessible in a single place”.

Possible methods of adaptation to the effects of climate change in the Tokaj Wine Region 

Viticulture’s adaptation to the harmful effects of climate change is globally the biggest challenge of the near future. Short, extremely intensive rainfalls and longer periods of drought are getting more frequent in the Tokaj Wine Region, where the majority of the vineyards are cultivated on steep slopes. Hence, erosion has high risk, especially when combined with the loess-based soils on about ten percent of the region. The environmentally beneficial cover crop and mulch usage can effectively reduce the risk of erosion, according to research done by the Tokaj Wine Region Research Institute of Viticulture and Oenology.

Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

The yeast ecosystem in grape musts is quite broad and depends on the region and the health of the grapes. Within this, there are yeasts that can generate fermentative deviations and/or cause defects in the wine. It is very important to address this issue because there are significant economic losses in the wine industry when the fermentation process and/or the organoleptic characteristics of the wine are negatively affected, even more today since climate change has a marked effect on the composition of this ecosystem. The aim of this work is to characterize the behavior regarding detrimental oenological features of potential spoilage yeasts isolated from viticultural environments.