terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Design of microbial consortia to improve the production of aromatic amino acid derived compounds during wine fermentation

Design of microbial consortia to improve the production of aromatic amino acid derived compounds during wine fermentation

Abstract

Wine contains secondary metabolites derived from aromatic amino acids (AADC), which can determine quality, stability and bioactivity. Several yeast species, as well as some lactic acid bacteria (LAB), can contribute in the production of these aromatic compounds. Winemaking should be studied as a series of microbial interactions, that work as an interconnected network, and can determine the metabolic and analytical profiles of wine. The aim of this work was to select microorganisms (yeast and LAB) based on their potential to produce AADC compounds, such as tyrosol and hydroxytyrosol, and design a microbial consortium that could increase the production of these AADC compounds in wines. Individual screenings of different strains of S. cerevisiae, non-Saccharomyces (non-Sac) and LAB where carried out in synthetic must with 5x of aromatic amino acids. Production of tyrosol and hydroxytyrosol was determined through HPLC-MS/MS. Two strains of S. cerevisiae, two of Zygosaccharomyces rouxii and two ofOenococcus oeni were selected for producing higher concentrations of tyrosol and hydroxytyrosol. Selected strains were then tested in different strategies of mixed inocula fermentations, combining the three microorganisms. Fermentations were done by single or co-inoculation of non-Sac and LAB strains, followed by sequential inoculation of S. cerevisiae strain. Organic acids, population dynamics and production of AADC were evaluated in the eight proposed consortia. Consortia that presented S. cerevisiae Lalvin CLOS and Z. rouxii CW96 produced highest concentrations of hydroxytyrosol (up to 3 µg/L). Moreover, all co-inoculations with LAB completed rapidly malolactic fermentations, and O. oeni did not increase acetic acid production. This study provides information on potential microbial interactions in microbial consortia that can enhance metabolic profile in winemaking.

Acknowledgements: This work has been financed by the project PDI2019-108722RB-C3. AS holds a fellowship of the Ministry of Science and Innovation (PRE2020-096645) and PGS a “Margarita Salas, María Zambrano, Recualificación” grant Spanish Ministry of Universities financed with European Union NextGenerationEU.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Andrea Silva1*, Sandra Martín-Esteban1, Pedro García-Serrano2, María-Jesús Torija1, Gemma Beltran1

1 Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, Tarragona, Spain
2 Department of Nutrition and Bromatology, Toxicology and Legal Medicine, Faculty of Pharmacy, Universidad de Sevilla, Sevilla, Spain

Contact the author*

Keywords

microbial consortia, hydroxytyrosol, yeast, lactic acid bacteria

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Multiple sclerosis (MS) is a multifactorial autoimmune disease associating demyelination and axonal degeneration developing in young adults and affecting 2–3 million people worldwide. Plant polyphenols endowed with many therapeutic benefits associated with anti-inflammatory and antioxidant properties represent highly interesting new potential therapeutic strategies. We recently showed the safety and high efficiency of grape seed extract (GSE), a complex mixture of polyphenolics compounds comprising notably flavonoids and proanthocyanidins, in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.

Energy partitioning and functionality of photosystem II in water-stressed grapevines during heatwaves revealed by continuous measurements of chlorophyll fluorescence

The increased intensity and frequency of heatwaves, coupled with prolonged periods of drought, are a significant threat to viticulture worldwide. During these conditions the more exposed leaves can show visible symptoms of heat damage. We monitored the functionality of photosystem II (PSII) in the field to better understand the impact of heatwaves on canopy performance. A factorial experiment was established in summer 2023 using Shiraz grapevines in the Barossa valley of South Australia, involving water-stressed and well-watered vines.

Foliar application of urea improved the nitrogen composition of Chenin grapes

The nitrogen composition of the grapes directly affects the developments of alcoholic fermentation and influences the final aromatic composition of the wines. The aim of this study was to determine the effect and efficiency of foliar applications of urea on the nitrogen composition of grapes. This study was carried out during 2023 vintage and in the Chenin vineyard located in Estacion Experimental Mendoza (Argentina). Three urea concentrations 3, 6 and 9 Kg N/ha (C1, C2, and C3, respectively) and control (T) were applied in this vineyard at veraison. In all solutions were added 1ml/l of Tween 80 ® surfactant.

Inert gases persistence in wine storage tank blanketing

It is common to find tanks in the winery with wine below their capacity due to wine transfers between tanks of different capacities or the interruption of operations for periods of a few days. This situation implies the existence of an ullage space in the tank with prolonged contact with the wine causing its absorption/oxidation. Oxygen uptake from the air headspace over the wine due to differences in the partial pressure of O2 can be rapid, up to 1.5 mL of O2 per liter of wine in one hour and 100 cm2 of surface area1 and up to saturation after 4 hours.

Evaluation of physiological properties of grapevine clones of ‘Tempranillo’ and ‘Graciano’ in DOCa Rioja (Spain)

In order to avoid the loss of grapevine intra-varietal diversity of DOCa Rioja grape varieties, Regional Government of La Rioja established a germplasm bank with more than 1.600 accessions, whose origin lies in the prospecting and sampling of ancient vineyards located throughout the whole region. 30 clones of Tempranillo and 13 clones of Graciano were preselected and multiplied in a new vineyard for further observations. The aim of this work is to describe the first results from the physiological characterization by an optical sensor of these preselected clones, which constitute the base of a new clonal selection that aims to increase the range of available certified clones and to improve the adaptation of these varieties to future objectives and environmental conditions.