terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Design of microbial consortia to improve the production of aromatic amino acid derived compounds during wine fermentation

Design of microbial consortia to improve the production of aromatic amino acid derived compounds during wine fermentation

Abstract

Wine contains secondary metabolites derived from aromatic amino acids (AADC), which can determine quality, stability and bioactivity. Several yeast species, as well as some lactic acid bacteria (LAB), can contribute in the production of these aromatic compounds. Winemaking should be studied as a series of microbial interactions, that work as an interconnected network, and can determine the metabolic and analytical profiles of wine. The aim of this work was to select microorganisms (yeast and LAB) based on their potential to produce AADC compounds, such as tyrosol and hydroxytyrosol, and design a microbial consortium that could increase the production of these AADC compounds in wines. Individual screenings of different strains of S. cerevisiae, non-Saccharomyces (non-Sac) and LAB where carried out in synthetic must with 5x of aromatic amino acids. Production of tyrosol and hydroxytyrosol was determined through HPLC-MS/MS. Two strains of S. cerevisiae, two of Zygosaccharomyces rouxii and two ofOenococcus oeni were selected for producing higher concentrations of tyrosol and hydroxytyrosol. Selected strains were then tested in different strategies of mixed inocula fermentations, combining the three microorganisms. Fermentations were done by single or co-inoculation of non-Sac and LAB strains, followed by sequential inoculation of S. cerevisiae strain. Organic acids, population dynamics and production of AADC were evaluated in the eight proposed consortia. Consortia that presented S. cerevisiae Lalvin CLOS and Z. rouxii CW96 produced highest concentrations of hydroxytyrosol (up to 3 µg/L). Moreover, all co-inoculations with LAB completed rapidly malolactic fermentations, and O. oeni did not increase acetic acid production. This study provides information on potential microbial interactions in microbial consortia that can enhance metabolic profile in winemaking.

Acknowledgements: This work has been financed by the project PDI2019-108722RB-C3. AS holds a fellowship of the Ministry of Science and Innovation (PRE2020-096645) and PGS a “Margarita Salas, María Zambrano, Recualificación” grant Spanish Ministry of Universities financed with European Union NextGenerationEU.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Andrea Silva1*, Sandra Martín-Esteban1, Pedro García-Serrano2, María-Jesús Torija1, Gemma Beltran1

1 Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, Tarragona, Spain
2 Department of Nutrition and Bromatology, Toxicology and Legal Medicine, Faculty of Pharmacy, Universidad de Sevilla, Sevilla, Spain

Contact the author*

Keywords

microbial consortia, hydroxytyrosol, yeast, lactic acid bacteria

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Analysis of volatile composition of interaction between the pathogen E. necator and two grapevine varieties

Volatile organic compounds (VOCs) are emitted by nearly all plant organs of the plants, including leaves. They play a key role in the communication with other organisms, therefore they are involved in plant defence against phytopathogens. In this study VOCs from grapevine leaves of two varieties of Vitis vinifera infected by Erysiphe necator were analysed. The varieties were selected based on their susceptibility to pathogen, Kishmish Vatkana has the Ren1 resistance gene and Zamarrica showed high susceptibility in previous trials.

Indicators of Sustainable Vineyard Soil Management: Metrics for Assessing Environmental Impacts

The vital role of soils in supporting life on our planet cannot be overstated. Soils provide numerous ecosystem services and functions, including biomass production, carbon sequestration, physical support, biological habitat, and genetic reserve, among others. Understanding the characteristics and sensitivity of soils in a specific terroir, along with effective soil management practices, is crucial for the sustainable management of natural resources.

Unveiling a hidden link: does time hold the key to altered spectral signatures of grapevines under drought?

Remote sensing technology captures spectral data beyond the visible range, making it useful for monitoring plant stress. Vis-NIR (Visible-Near Infrared) spectroscopy (400-1000 nm) is commonly used to indirectly assess plant status during drought. One example is the widespread use of normalized difference vegetation index (NDVI) that is strongly linked to green biomass. However, a knowledge gap exists regarding the applicability of this method to all the drought conditions and if it is a direct correlation to the water status of the plant.

Wine racking in the winery and the use of inerting gases

The O2 uptake in the different winemaking processes is generally considered to be negative for the sensory characteristics of white and rosé wines. Wine racking is a critical point of O2 uptake, as the large surface area of the wine exposed during this operation and the inability to maintain an effective inert gas blanket over it.
The objective was to study O2 uptake during the racking of a model wine without using inert gases and to compare it with the purging of the destination tank with different inert gases.

Application of UV-B radiation in pre- and postharvest as an innovative and sustainable cultural practice to improve grape phenolic composition

Ultraviolet radiation (UVR) is a minor part of the solar spectrum, but it represents an important ecological factor that influences many biological processes related to plant growth and development. In recent years, the application of UVR in agriculture and food production is emerging as a clean and environmentally friendly technology.
In grapevine, many studies have been conducted on the effects of ambient levels of UVR, but there are few considering the effects of UV-B application on grape phenolic composition under commercial growing or postharvest conditions.