terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Design of microbial consortia to improve the production of aromatic amino acid derived compounds during wine fermentation

Design of microbial consortia to improve the production of aromatic amino acid derived compounds during wine fermentation

Abstract

Wine contains secondary metabolites derived from aromatic amino acids (AADC), which can determine quality, stability and bioactivity. Several yeast species, as well as some lactic acid bacteria (LAB), can contribute in the production of these aromatic compounds. Winemaking should be studied as a series of microbial interactions, that work as an interconnected network, and can determine the metabolic and analytical profiles of wine. The aim of this work was to select microorganisms (yeast and LAB) based on their potential to produce AADC compounds, such as tyrosol and hydroxytyrosol, and design a microbial consortium that could increase the production of these AADC compounds in wines. Individual screenings of different strains of S. cerevisiae, non-Saccharomyces (non-Sac) and LAB where carried out in synthetic must with 5x of aromatic amino acids. Production of tyrosol and hydroxytyrosol was determined through HPLC-MS/MS. Two strains of S. cerevisiae, two of Zygosaccharomyces rouxii and two ofOenococcus oeni were selected for producing higher concentrations of tyrosol and hydroxytyrosol. Selected strains were then tested in different strategies of mixed inocula fermentations, combining the three microorganisms. Fermentations were done by single or co-inoculation of non-Sac and LAB strains, followed by sequential inoculation of S. cerevisiae strain. Organic acids, population dynamics and production of AADC were evaluated in the eight proposed consortia. Consortia that presented S. cerevisiae Lalvin CLOS and Z. rouxii CW96 produced highest concentrations of hydroxytyrosol (up to 3 µg/L). Moreover, all co-inoculations with LAB completed rapidly malolactic fermentations, and O. oeni did not increase acetic acid production. This study provides information on potential microbial interactions in microbial consortia that can enhance metabolic profile in winemaking.

Acknowledgements: This work has been financed by the project PDI2019-108722RB-C3. AS holds a fellowship of the Ministry of Science and Innovation (PRE2020-096645) and PGS a “Margarita Salas, María Zambrano, Recualificación” grant Spanish Ministry of Universities financed with European Union NextGenerationEU.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Andrea Silva1*, Sandra Martín-Esteban1, Pedro García-Serrano2, María-Jesús Torija1, Gemma Beltran1

1 Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, Tarragona, Spain
2 Department of Nutrition and Bromatology, Toxicology and Legal Medicine, Faculty of Pharmacy, Universidad de Sevilla, Sevilla, Spain

Contact the author*

Keywords

microbial consortia, hydroxytyrosol, yeast, lactic acid bacteria

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Climate change and viticulture in Nordic Countries and the Helsinki area

The first vineyards in Northern Europe were in Denmark in the 15th century, in the southern parts of Sweden and Finland in the 18th century at 55–60 degrees latitude. The grapes grown there have not been made into wine, but the grapes have been eaten at festive tables. The resurgence of viticulture has started with global warming, and currently the total area of viticulture in the Nordic countries, including Norway, is estimated to be 400–500 hectares, most of which is in Denmark. Southern Finland, like all southern parts of Northern Europe, belongs to the cool-cold winegrowing area.

Wine without added SO2: Oxygen impact and color evolution during red wine aging

SO2 play a major role in wine stability and evolution during its aging and storage. Winemaking without SO2 is a big challenge for the winemakers since the lack of SO2 affects directly the wine chemical evolution such as the aromas compounds as well as the phenolic compounds. During the red wine aging, phenolic compounds such as anthocyanin, responsible of the red wine colour, and tannins, responsible of the mouthfeel organoleptic properties of wine, evolved quickly from the winemaking process to aging [1]. A lot of new interaction and molecules occurred lead by oxygen[2] thus the lack of SO2 will induce wine properties changes. Nowadays, the phenolic composition of the wine without added SO2 have not been clearly reported.

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.
We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin.

Evaluation of the effects of pruning methodology on the development of young vines 

Grapevine pruning is one of the most important practices in the vineyards. Winegrowers use it to provide the vines the shape needed, or to maintain it once achieved, and also to balance vegetative growth and fruit production. In the last decades, careless pruning has been blamed, among other factors, as responsible of the vineyard decay that is been observed even in young vines. However, to our knowledge, there is a lack of systematic research trying to elucidate to which extent the pruning method used affects plant development or its susceptibility to grapevine trunk diseases (GTD). Within this context, the aim of this work is to study the influence of different pruning method strategies on the development of field-planted young vines.

Sustainable management of grapevine trunk diseases

Grapevine trunk diseases (GTD) occur wherever grapes are grown and are considered the main biotic factor reducing yields and shortening vineyards’ lifespan. Currently, no product is available to eradicate GTD once grapevines are infected. Therefore, prophylactic strategies based on pruning wound protection and ‘remedial surgery’, the only eradication method based on the elimination of infected wood and renewal of the vine by means of new canes or suckers, are the only effective strategies available. The Canadian grape and wine industry focusses on a sustainable production and thus, looking for alternatives to chemicals for disease management is a top priority.