terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Oenological compatibility of biocontrol yeasts applied to wine grapes 

Oenological compatibility of biocontrol yeasts applied to wine grapes 

Abstract

Antagonistic yeasts applied to wine grapes must be compatible with the thereafter winemaking process, avoiding competition with the fermentative Saccharomyces cerevisiae or affecting wine flavour. Therefore, fifteen epiphytic yeasts (6 Metschnikowia sp., 6 Hanseniaspora uvarum, 3 Starmerella bacillaris) previously selected for its biocontrol ability against Alternaria on wine grapes were evaluate for possible competition with S. cerevisiae by the Niche Overlap Index (NOI) employing YNB agar media with 10 mM of 17 different carbonate sources present in wine grapes (proline, asparagine, alanine, glutamic acid, tirosine, arginine, lisine, methionine, glicine, malic acid, tartaric acid, fructose, melibiose, raffinose, rhamnose, sucrose, glucose). Also, acetic acid production in YPD-calcium carbonate agar medium, H2S production in Biggy agar medium and the production of “Brett” character or other odour defects in pasteurized must with p-coumaric acid were evaluated. As result, all Metschnikowia sp. strains showed coexistence with S. cerevisiae because the NOI (number of common carbon sources used by the two microorganisms/number of carbon sources used only by the antagonist) was <0.9 (0.42). Meanwhile, all H. uvarum and S. bacillaris strains showed competition with S. cerevisiae (NOI=1) and between them (NOI=1). On the other hand, almost all H. uvarum(5 of 6) and all S. bacillaris strains produced acetic acid, whereas none of the Metschnikowia sp. showed acetic acid production. All the yeasts analysed produced H2S in Biggy agar medium. Nevertheless, the majority of them exhibited no defect or in certain cases a slight solvent or acetate odour (different from “Brett character”) in must. Therefore, among all the biocontrol yeasts evaluated, Metschinikowia sp. strains showed more compatibility features than the rest of the strains and its oenological behaviour should be further studied under vinification conditions.

Acknowledgements: Marie Sklodowska-Curie Research and Innovation Staff Exchange project (872394-vWISE-H2020-MSCA-RISE-2019) – European Comission.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Luciana Paola Prendes1*, María Gabriela Merín 1, Claire Courtel2, Carina Morales3, Juliana Garau1, Vilma Inés Morata de Ambrosini 1

1ICAI (Instituto de Ciencias Aplicadas a la Industria, CONICET-Facultad de Ciencias Aplicadas, UNCuyo), Bernardo de Irigoyen 375, San Rafael (Mendoza), Argentina.
2Oniris (École nationale vétérinaire, agroalimentaire et de l’alimentation de Nantes-Atlantique), Rue de la Géraudière, CS 82225, 44322 Nantes, France.
3Facultad de Ciencias Aplicadas (UNCuyo), Bernardo de Irigoyen 375, San Rafael (Mendoza), Argentina.

Contact the author*

Keywords

biocontrol yeasts, NOI, detrimental oenological characters

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Barrels ad-hoc: Spanish oak wood classification by NIRs 

The wooden barrel is a key factor in enology, since wine chemical composition and sensory properties changes significantly in contact with the barrel[1]. Today’s highly competitive market constantly demands new differentiated products and wineries search innovations continuously.
Wood selection is crucial: barrels stability to keep constant their contribution and the result on products, and additional and differentiated wood contributions to impact their new products. Oak wood selection has traditionally been carried out using parameters such as specie, location and grain, however, it goes one step further nowadays. Large cooperage work with non-destructive techniques that allow classifying oak wood quickly and easily according to their organoleptic contribution[2].

Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Leaf removal in the fruit-zone has been employed to improve cluster light exposure and ventilation and therefore increase metabolite accumulation and reduce botrytis incidence in berries. When applied before flowering (early defoliation – ED), it can also decrease cluster compactness and regulate yield in high-yielding varieties. This study aimed to evaluate the impact of ED on the physiology and metabolism of Aragonez (syn. Tempranillo) berries along the ripening period. The experiment was set up in 2013 at a commercial vineyard located in the Lisbon winegrowing region.

Quantification of polysaccharides of variety Pomaces of the D.O.Ca Rioja

Pomace is one of the main residues generated by the wine industry and represents an environmental problem. Currently, there is a growing interest in the revaluation of these products because different bioactive compounds can be obtained from them, such as polyphenols, grape seed oils and polysaccharides. Red grape pomace can be an important source of polysaccharides, but they are currently little studied and even less with viable and environmental extraction processes (green extraction), such as flash extraction. The residual amount of the fraction rich in pectin (residual pulp) and component rich in hemicellulose in the pomace and the strength of association of the pectin with the cellulose-xyloglucan network depend on the degree of extractability of the polysaccharides in red winemaking and on the winemaking conditions.

Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Grapevine cultivation is threatened by the global warming, which combines high temperatures and reduced rainfall, impacting in wine quality and even plant survival. Breeding for varieties resilient to these challenges must address plant traits such as tolerance to supraoptimal temperatures and optimized water use efficiency while minimizing productivity and quality losses. Stomatal abundance (SA) determines the maximum leaf potential for transpiration and thus water loss and cooling. Since SA results from a developmental process during leaf emergence and growth, knowledge on the genetic control of this process would provide specific targets for modification.

Effect of soil particle size on vine water status, leaf ABA content and berry quality in Nebbiolo grapes

The root and shoot abscisic acid (ABA) accumulation in response to water deficit and its relation with stomatal conductance is longtime known in grapevine. ABA-dependent and ABA-independent signalling response to osmotic stress coexist in sessile plants. In grapevine, the signaling role of ABA in response to water stress conditions and its influence on berry quality is critical to manage grapevine acclimation to climate change.