terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Oenological compatibility of biocontrol yeasts applied to wine grapes 

Oenological compatibility of biocontrol yeasts applied to wine grapes 

Abstract

Antagonistic yeasts applied to wine grapes must be compatible with the thereafter winemaking process, avoiding competition with the fermentative Saccharomyces cerevisiae or affecting wine flavour. Therefore, fifteen epiphytic yeasts (6 Metschnikowia sp., 6 Hanseniaspora uvarum, 3 Starmerella bacillaris) previously selected for its biocontrol ability against Alternaria on wine grapes were evaluate for possible competition with S. cerevisiae by the Niche Overlap Index (NOI) employing YNB agar media with 10 mM of 17 different carbonate sources present in wine grapes (proline, asparagine, alanine, glutamic acid, tirosine, arginine, lisine, methionine, glicine, malic acid, tartaric acid, fructose, melibiose, raffinose, rhamnose, sucrose, glucose). Also, acetic acid production in YPD-calcium carbonate agar medium, H2S production in Biggy agar medium and the production of “Brett” character or other odour defects in pasteurized must with p-coumaric acid were evaluated. As result, all Metschnikowia sp. strains showed coexistence with S. cerevisiae because the NOI (number of common carbon sources used by the two microorganisms/number of carbon sources used only by the antagonist) was <0.9 (0.42). Meanwhile, all H. uvarum and S. bacillaris strains showed competition with S. cerevisiae (NOI=1) and between them (NOI=1). On the other hand, almost all H. uvarum(5 of 6) and all S. bacillaris strains produced acetic acid, whereas none of the Metschnikowia sp. showed acetic acid production. All the yeasts analysed produced H2S in Biggy agar medium. Nevertheless, the majority of them exhibited no defect or in certain cases a slight solvent or acetate odour (different from “Brett character”) in must. Therefore, among all the biocontrol yeasts evaluated, Metschinikowia sp. strains showed more compatibility features than the rest of the strains and its oenological behaviour should be further studied under vinification conditions.

Acknowledgements: Marie Sklodowska-Curie Research and Innovation Staff Exchange project (872394-vWISE-H2020-MSCA-RISE-2019) – European Comission.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Luciana Paola Prendes1*, María Gabriela Merín 1, Claire Courtel2, Carina Morales3, Juliana Garau1, Vilma Inés Morata de Ambrosini 1

1ICAI (Instituto de Ciencias Aplicadas a la Industria, CONICET-Facultad de Ciencias Aplicadas, UNCuyo), Bernardo de Irigoyen 375, San Rafael (Mendoza), Argentina.
2Oniris (École nationale vétérinaire, agroalimentaire et de l’alimentation de Nantes-Atlantique), Rue de la Géraudière, CS 82225, 44322 Nantes, France.
3Facultad de Ciencias Aplicadas (UNCuyo), Bernardo de Irigoyen 375, San Rafael (Mendoza), Argentina.

Contact the author*

Keywords

biocontrol yeasts, NOI, detrimental oenological characters

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Foliar application of urea improved the nitrogen composition of Chenin grapes

The nitrogen composition of the grapes directly affects the developments of alcoholic fermentation and influences the final aromatic composition of the wines. The aim of this study was to determine the effect and efficiency of foliar applications of urea on the nitrogen composition of grapes. This study was carried out during 2023 vintage and in the Chenin vineyard located in Estacion Experimental Mendoza (Argentina). Three urea concentrations 3, 6 and 9 Kg N/ha (C1, C2, and C3, respectively) and control (T) were applied in this vineyard at veraison. In all solutions were added 1ml/l of Tween 80 ® surfactant.

Inert gases persistence in wine storage tank blanketing

It is common to find tanks in the winery with wine below their capacity due to wine transfers between tanks of different capacities or the interruption of operations for periods of a few days. This situation implies the existence of an ullage space in the tank with prolonged contact with the wine causing its absorption/oxidation. Oxygen uptake from the air headspace over the wine due to differences in the partial pressure of O2 can be rapid, up to 1.5 mL of O2 per liter of wine in one hour and 100 cm2 of surface area1 and up to saturation after 4 hours.

Genetic variation among wild grapes native to Japan

Domesticated grapes are assumed to have originated in the Middle East. However, a considerable number of species are native in East Asian countries such as China, Korea and Japan as well. Evidence suggests that a total of seven species and eight varieties have been found to be native to Japan. A wide level variation in morphology, genetic and fruit composition exist in wild grape native to Japan.

Identification of several glycosidic aroma precursors in six varieties of winemaking grapes and assessment of their aroma potential by acid hydrolysis

In winemaking grapes, it is known that most aroma compounds are present as non-volatile precursors, such as glycosidic precursors. In fact, there is strong evidence supporting the connection between the content of aroma precursors and the aromatic quality of wine [1]. Acid hydrolysis is preferred to reveal the aroma potential of winemaking grapes, as it predicts more accurately the chemical rearrangements occurring during fermentation in acidic environments [2]. In this study, a method involving a fast fermentation followed by acid hydrolysis at 75ºC was used to evaluate the accumulation of aroma compounds over time in fractions obtained from six different varieties of winemaking grapes.

Model-assisted analysis of the root traits underlying RSA genotypic diversity in Vitis: a promising approach for rootstock selection?

By dissecting the root system architecture (RSA) into its underpinning components (e.g. root emission, axial growth, radial growth, branching, root direction or tropism) and identifying the relationships between them, functional-structural 3D root models are promising tools for analyzing the diversity and complexity of root system phenotypes with Genotype × Environment interactions. The model parameters are assumed to be synthetic traits, less influenced by the environment, and consequently with less polygenic architectures than the integrative RSA traits they drive. Root models can serve as a basis for in silico development of root system ideotypes by highlighting the developmental processes and parameters that most likely influence RSA fitness.