terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Oenological compatibility of biocontrol yeasts applied to wine grapes 

Oenological compatibility of biocontrol yeasts applied to wine grapes 

Abstract

Antagonistic yeasts applied to wine grapes must be compatible with the thereafter winemaking process, avoiding competition with the fermentative Saccharomyces cerevisiae or affecting wine flavour. Therefore, fifteen epiphytic yeasts (6 Metschnikowia sp., 6 Hanseniaspora uvarum, 3 Starmerella bacillaris) previously selected for its biocontrol ability against Alternaria on wine grapes were evaluate for possible competition with S. cerevisiae by the Niche Overlap Index (NOI) employing YNB agar media with 10 mM of 17 different carbonate sources present in wine grapes (proline, asparagine, alanine, glutamic acid, tirosine, arginine, lisine, methionine, glicine, malic acid, tartaric acid, fructose, melibiose, raffinose, rhamnose, sucrose, glucose). Also, acetic acid production in YPD-calcium carbonate agar medium, H2S production in Biggy agar medium and the production of “Brett” character or other odour defects in pasteurized must with p-coumaric acid were evaluated. As result, all Metschnikowia sp. strains showed coexistence with S. cerevisiae because the NOI (number of common carbon sources used by the two microorganisms/number of carbon sources used only by the antagonist) was <0.9 (0.42). Meanwhile, all H. uvarum and S. bacillaris strains showed competition with S. cerevisiae (NOI=1) and between them (NOI=1). On the other hand, almost all H. uvarum(5 of 6) and all S. bacillaris strains produced acetic acid, whereas none of the Metschnikowia sp. showed acetic acid production. All the yeasts analysed produced H2S in Biggy agar medium. Nevertheless, the majority of them exhibited no defect or in certain cases a slight solvent or acetate odour (different from “Brett character”) in must. Therefore, among all the biocontrol yeasts evaluated, Metschinikowia sp. strains showed more compatibility features than the rest of the strains and its oenological behaviour should be further studied under vinification conditions.

Acknowledgements: Marie Sklodowska-Curie Research and Innovation Staff Exchange project (872394-vWISE-H2020-MSCA-RISE-2019) – European Comission.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Luciana Paola Prendes1*, María Gabriela Merín 1, Claire Courtel2, Carina Morales3, Juliana Garau1, Vilma Inés Morata de Ambrosini 1

1ICAI (Instituto de Ciencias Aplicadas a la Industria, CONICET-Facultad de Ciencias Aplicadas, UNCuyo), Bernardo de Irigoyen 375, San Rafael (Mendoza), Argentina.
2Oniris (École nationale vétérinaire, agroalimentaire et de l’alimentation de Nantes-Atlantique), Rue de la Géraudière, CS 82225, 44322 Nantes, France.
3Facultad de Ciencias Aplicadas (UNCuyo), Bernardo de Irigoyen 375, San Rafael (Mendoza), Argentina.

Contact the author*

Keywords

biocontrol yeasts, NOI, detrimental oenological characters

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Exploring intra-vineyard variability with sensor- and molecular-based approaches 

The application of remote and proximal sensing is a fast and efficient method to monitor grapevine vegetative and physiological parameters and is considered valuable to derive information on associated yield and quality traits in the vineyard. Further details can be obtained by the application of molecular analysis at the gene expression level aiming at elucidating how pathways controlling the formation of different grape quality traits are influenced by spatial variability. This work aims at evaluating intra-vineyard variability in grape composition at harvest and at comparing this with remotely sensed canopy vegetation data and molecular-based approaches.

Retrospective analysis of our knowledge regarding the genetics of relevant traits for rootstock breeding 

Rootstocks were the first sustainable and environmentally friendly strategy to cope with a major threat for Vitis vinifera cultivation. In addition to providing Phylloxera resistance, they play an important role in protecting against other soil-borne pests, such as nematodes, and in adapting V. vinifera to limiting abiotic conditions. Today viticulture has to adapt to ongoing climate change whilst simultaneously reducing its environmental impact. In this context, rootstocks are a central element in the development of agro-ecological practices that increase adaptive potential with low external inputs. Despite the apparent diversity of the Vitis genus, only few rootstock varieties are used worldwide and most of them have a very narrow genetic background. This means that there is considerable scope to breed new, improved rootstocks to adapt viticulture for the future.

Antimicrobial activity of oenological polyphenols against Gram positive and Gram negative intestinal multidrug-resistant bacteria

Bacterial antibiotic resistance is a major current health problem. Polyphenols have demonstrated antibacterial activity, and in this work we studied the effect of oenological polyphenols on the growth of intestinal multidrug-resistant strains of human and animal origin. Two Enterococcus faecium strains, resistant to vancomycin and other antibiotics, and four Escherichia coli strains, resistant to ampicillin and other antibiotics, were included in this study. All strains showed multidrug resistant phenotypes and genotypes to at least two antibiotic families.

Effect of two water deficit regimes on the agronomic response of 12 grapevine varieties cultivated in a semi-arid climate

The Mediterranean basin is one of the most vulnerable regions to Climate Change effects. According to unanimous forecasts, the vineyards of Castilla-La Mancha will be among the most adversely affected by rising temperatures and water scarcity during the vine’s vegetative period. One potential strategy to mitigate the negative impacts of these changes involves the identification of grapevine varieties with superior water use efficiency, while ensuring satisfactory yields and grape quality.

The environmental footprint of selected vineyard management practices: A case study from Logroño (La Rioja) Spain

Viticulture is globally important for socioeconomic and environmental reasons. The EU is globally leading grape and wine production, and Spain is among the top grape and wine producers. As climate change affects viticulture, mitigation and adaptation are crucial for protecting grape production. In this research work, data on viticultural management practices such as soil cultivation, irrigation, energy, machinery, plant protection and the use of fertilizers from vineyards located in Logroño (La Rioja) have been obtained.