terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Mycorrhizal symbiosis modulates flavonoid and amino acid profiles in grapes of Tempranillo and Cabernet Sauvignon 

Mycorrhizal symbiosis modulates flavonoid and amino acid profiles in grapes of Tempranillo and Cabernet Sauvignon 

Abstract

Arbuscular mycorrhizal fungi (AMF) symbiosis is probably the most widespread beneficial interaction between plants and microorganisms. AMF has been widely reported to promote grapevine growth, water and nutrient uptake as well as both biotic and abiotic stress tolerance[1]. However, the impact of AMF on grape composition has been less studied. The aim of this work was to evaluate the effects of the association between two commercial grapevine cultivars (Tempranillo and Cabernet Sauvignon grafted onto 110 rootstock) and AMF on the anthocyanin, flavonol and amino acid concentrations and profiles of grapes. Two-year old grapevine plants were grown in 13 L pots, in a greenhouse under semi-controlled environmental conditions. Half of the plants of each variety were inoculated with a consortium of five AMF fungi at the vegetative stage. The concentrations of the main anthocyanins, flavonols and amino acids were analyzed in mature grapes using HPLC-DAD. Mycorrhizal inoculation barely influenced the concentration and profile of anthocyanins and flavonols in the varieties studied. However, AMF increased the concentration of total amino acids by 49%, in particular a-ketoglutarate, aspartate and shikimate derivatives, as well as the levels of aromatic precursor amino acids by 71%. Such effect of AMF was more evident in Cabernet Sauvignon than in Tempranillo. The results suggest that the inoculation of grapevine with AMF can improve the amino acid composition of grapes, which may have a potential impact on the wine-making process and the aromatic characteristics of wine.

Acknowledgements: A. Urdiain, M. Oyarzun & H. Santesteban for technical support, Asociación de Amigos UNAV (D. Kozikova’s scholarship), Bioera SL for AMF, MICINN (Gobierno España) (Ref. PID2020-118337RB-IOO) and ANDIA Talent Senior grant (Gobierno de Navarra).

References:

1) Trouvelot S. et al. (2015) Arbuscular mycorrhiza symbiosis in viticulture: a review. Agron. Sustain. Dev., 35: 1449-1467, DOI 10.1007/s13593-015-0329-7

DOI:

Publication date: October 16, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Daria Kozikova1*#, Johann Martínez-Lüscher#, Nieves Goicoechea, Inmaculada Pascual

1Instituto BIOMA-Universidad de Navarra, C/ Irunlarrea, 1. 31008, Pamplona (SPAIN)

#Both authors contributed equally

Contact the author*

Keywords

amino acids, anthocyanins, flavonols, grape, mycorrhizal symbiosis, Vitis vinifera L.

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Comparison of the effects of hormone- and natural-based elicitors on key metabolic pathways in cv. Tempranillo

One of the most important effects of climate change in wine-growing areas is the advance of phenological stages, especially concerning early berry ripening. In the hottest seasons, this results in a lack of synchrony between sugar and phenolic ripeness. In order to cope with this fact, a general effort is being made by researchers and growers aiming at delaying ripening through different strategies. One of the proposed approaches is the application of elicitors. This study aims to assess the effect at the transcriptomic level of application of three elicitors (Vitalfit, Fruitel, and Protone) in Tempranillo.

Wine without added SO2: Oxygen impact and color evolution during red wine aging

SO2 play a major role in wine stability and evolution during its aging and storage. Winemaking without SO2 is a big challenge for the winemakers since the lack of SO2 affects directly the wine chemical evolution such as the aromas compounds as well as the phenolic compounds. During the red wine aging, phenolic compounds such as anthocyanin, responsible of the red wine colour, and tannins, responsible of the mouthfeel organoleptic properties of wine, evolved quickly from the winemaking process to aging [1]. A lot of new interaction and molecules occurred lead by oxygen[2] thus the lack of SO2 will induce wine properties changes. Nowadays, the phenolic composition of the wine without added SO2 have not been clearly reported.

A sensometabolomic approach to understand wine mouthfeel percepts

Targeted analytical methods can overlook compounds that are a priori unknown to play a role in the mouthfeel sensations. This limitation can be overcome with the information provided by untargeted metabolomic analysis using UPLC‐QTOF-MS. To this end, an untargeted metabolomic approach applied to 42 red wines has allowed development of a model with predictive capacity by cross-validation for the “dry”, “oily” and “unctuous” sensations perceived by a sensory panel. The optimal PLS model for “dry” retained compounds with positive regression coefficients (≥ 0.17) including a trimer procyanidin, a peptide, and four anthocyanins.

Identification of a stable epi-allele associated with flower development and low bunch compactness in a somatic variant of Tempranillo Tinto

Grapevine cultivars are vegetatively propagated to preserve their varietal characteristics. However, spontaneous somatic variations that occur and are maintained during cycles of vegetative growth offer opportunities for the natural improvement of traditional grape cultivars. One advantageous trait for winegrowing is reduced bunch compactness, which decreases the susceptibility to pests and fungal diseases and favor an even berry ripening.

Predicting provenance and grapevine cultivar implementing machine learning on vineyard soil microbiome data: implications in grapevine breeding

The plant rhizosphere microbial communities are an essential component of plant microbiota, which is crucial for sustaining the production of healthy crops. The main drivers of the composition of such communities are the growing environment and the planted genotype. Recent viticulture studies focus on understanding the effects of these factors on soil microbial composition since microbial biodiversity is an important determinant of plant phenotype, and of wine’s organoleptic properties. Microbial biodiversity of different wine regions, for instance, is an important determinant of wine terroir.