terclim by ICS banner
IVES 9 IVES Conference Series 9 Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Abstract

Microbial ecosystems are primary drivers of viticultural, oenological and other cellar-related processes such as wastewater treatment. Metagenomic datasets have broadly mapped the vast microbial species diversity of many of the relevant ecological niches within the broader wine environment, from vineyard soils to plants and grapes to fermentation. The data highlight that species identities and diversity significantly impact agronomic performance of vineyards as well as wine quality, but the complexity of these systems and of microbial growth dynamics has defeated attempts to offer actionable tools to guide or predict specific outcomes of ecosystem-based interventions. The application of such tools in future will depend on our understanding of the physiological and molecular drivers that govern microbial ecosystems. Here we describe several integrated approaches to characterize the molecular interactions between species within the fermentation and the waste-water ecosystem and to model the development of these ecosystems. Binary (two species) and consortia-based approaches indicate ecosystem-specific developmental patterns in these systems. On a molecular level, data strongly support that cell-wall related properties of yeast species impact the development of fermentation ecosystems during wine making and highlight the importance of physical contacts between species in these ecological processes. To model the wine yeast fermentation ecosystem, high-throughput flow cytometry-based approaches were developed, and specific models based on a machine-learning approach were developed. In winery wastewater, laboratory-based evolution of two species exposed to biotic selection pressure in a synthetic environment, Saccharomyces cerevisiae and the microalga Chlorella sorokiniana, identified two specific genes involved in carbon and nitrogen catabolite repression that facilitate mutualistic behaviors between yeast and microalgae when inactive. Taken together the data suggest novel strategies for microbial ecosystem-based decision making in wine making and improved integration of natural microbial biodiversity in the process.

DOI:

Publication date: 11/02/2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Florian F. Bauer, Cleo Conacher, Jennifer Oosthuizen, Georgia Strydom, Evodia Setati, Rene Naidoo-Blassoples

South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University, Stellenbosch, South Africa

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

MOVING FROM SULFITES TO BIOPROTECTION: WHICH IMPACT ON CHARDONNAY WINE?

Over the last few years, several tools have been developed to reduce the quantity of sulfites used during winemaking, including bioprotection. Although its effectiveness in preventing the development of spoilage microorganisms has been proven, few data are available on the impact of sulfite substitution by bioprotection on the final product. The objective of this study was therefore to characterize Chardonnay wines with the addition of sulfite or bioprotection in the pre-fermentation stage. The effects of both treatments on resulting matrices was evaluated at several scales: analysis of classical oenological parameters, antioxidant capacity, phenolic compounds, non-volatile metabolome and sensory profile.

NEW TOOL FOR SIMULTANEOUS MEASUREMENT OF OXYGEN CONSUMPTION AND COLOUR MODIFICATIONS IN WINES

Measuring the effect of oxygen consumption on the colour of wines as the level of dissolved oxygen decreases over time is very useful to know how much oxygen a wine is able to consume without significantly altering its colour. The changes produced in wine after being exposed to high oxygen concen-trations have been studied by different authors, but in all cases the wine has been analysed once the oxygen consumption process has been completed. This work presents the results obtained with the use of an equipment designed and made to measure simultaneously the level of dissolved oxygen and the spectrum of the wine, during the oxygen consumption process from saturation levels with air to very low levels, which indicate the total consumption of the dosed oxygen.

NOVEL BENZENETHIOLS WITH PHENOLS CAUSE ASHY, SMOKE FLAVOR PERCEPTION IN RED WINES

Smoke impacts on wines are becoming a worldwide problem; the size and severity of wildfires increasing due to influences from changing climates.¹ For over a century, wines have been known to have a unique issue of absorbing chemical compounds derived from wildfire smoke wherein the flavor of the subsequent wine becomes ashy, rubbery, campfire-like, and smoky.² The economic impacts of a smoke-impacted wine can last for years depending on the grape varietal, costing Oregon and Washington states in the United States over a billion dollars from the 2020 wildfires, as an example.³ While years of research have indicated elevated concentrations of smoke-related compounds, such as guaiacol and syringol, in wines after smoke events, unfortunately, replicating the sensory experience using smoke-associated phenols has not had much success.⁴

ANALYZING THE ROLE OF ELEMENTAL SULFUR IN GRAPE JUICE ON THE DEVELOPMENT OF POLYFUNCTIONAL MERCAPTANS IN SAUVIGNON BLANC WINES

Sauvignon blanc is characterized by distinctive aromas, both fruity and herbaceous. The “green” character has been attributed to the methoxypyrazines, while the “fruity” character is associated with polyfunctional mercaptans . Polyfunctional mercaptans are of great significance due to their high impact on wines and associated low perception thresholds.
Elemental sulfur (S⁰) is widely used to protect grapevines from powdery mildew.

PREVALENCE OF OAK-RELATED AROMA COMPOUNDS IN PREMIUM WINES

Barrel fermentation and barrel-ageing of wine are commonly utilised practices in premium wine production. The wine aroma compounds related to barrel contact are varied and can enhance a range of wine aromas and flavours, such as ‘struck flint’, ‘caramel’, ‘red berry’, ‘toasty’ and ‘nutty’, as well as conventional oaky characters such as ‘vanilla’, ‘spice’, ‘smoky’ and ‘coconut’. A survey of commercially produced premium Shiraz, Cabernet Sauvignon, Pinot Noir and Chardonnay wines was conducted, assessing the prevalence of compounds that have been proposed as barrel-ageing markers¹ including oak lactones, volatile phenols, furanones, aldehydes, thiazoles2,3, phenylmethanethiol⁴ and 2-furylmethanethiol.⁵