terclim by ICS banner
IVES 9 IVES Conference Series 9 PROBING GRAPEVINE-BOTRYTIS CINEREA INTERACTION THROUGH MASS SPECTROMETRY IMAGING

PROBING GRAPEVINE-BOTRYTIS CINEREA INTERACTION THROUGH MASS SPECTROMETRY IMAGING

Abstract

Plants in their natural environment are in continuous interaction with large numbers of potentially pathogenic and beneficial microorganisms. Depending on the microbe, plants have evolved a variety of resistance mechanisms that can be constitutively expressed or induced. Phytoalexins, which are biocidal compounds of low to medium molecular weight synthesized by and accumulated in plants as a response to stress, take part in this intricate defense system.1,2

One of the limitations of our knowledge of phytoalexins is the difficulty of analyzing their spatial responsiveness occurring during plant- pathogen interactions under natural conditions.

Mass spectrometry imaging is an innovative analytical technique for the spatial mapping of molecules within a sample. It has the ability to provide valuable insights into the molecular processes underlying the interaction between a plant and a pathogen. Despite its advantages, to date, the studies of the application of MALDI-MSI to plants is not extensive, even more the ones investigating grapevine compounds.3–5

In a first part, the presentation will focus on the principle and characteristic data provided by this methodology involving a localized ion source and a high-resolution mass spectrometer. The following part will be dedicated to our work on the application of this methodology to investigate phytoalexin accumulation and distribution in leaf tissues of Vitis vinifera cv Chardonnay, infected with Botrytis cinerea, the causal agent for gray mold. We specifically investigate the accumulation and spatial distribution of resveratrol and its derivative oligomers through a time course infection using matrix-assisted laser desorption ionization – mass spectrometry imaging (MALDI-MSI). Our results show that production of stilbene phytoalexins is rapidely activated by the presence of the pathogen, with a high molecular diversity as evidenced by the detection of various oligomeric forms. Moreover, the specific behavior of these compounds towards the propagation of the infection by the pathogen is clearly highlighted at the micron scale.

 

1. Jeandet, P.; Delaunois, B.; Conreux, A.; Donnez, D.; Nuzzo, V.; Cordelier, S.; Clément, C.; Courot, E. Biosynthesis, Metabolism, Molecular Engineering, and Biological Functions of Stilbene Phytoalexins in Plants. BioFactors 2010, 36 (5), 331–341.
2. Jeandet, P.; Uddin, M. S.; Clément, C.; Aziz, A.; Jacquard, C.; Khan, H.; Shah, M. A.; Barka, E. A.; Koffas, M.; Nabavi, S. M.; Sobarzo-Sánchez, E.; Renault, J.-H. Production of High Molecular-Ordered Stilbene Oligomers for the Study of Their Biological Activity: Total Synthesis, Bio-Catalyzed Synthesis and Production by Plant Systems. Nat. Prod. Rep. 2023.
3. Becker, L.; Carré, V.; Poutaraud, A.; Merdinoglu, D.; Chaimbault, P. MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves. Molecules 2014, 19 (7), 10587–10600.
4. Maia, M.; McCann, A.; Malherbe, C.; Far, J.; Cunha, J.; Eiras-Dias, J.; Cordeiro, C.; Eppe, G.; Quinton, L.; Figueiredo, A.; De Pauw, E.; Sousa Silva, M. Grapevine Leaf MALDI-MS Imaging Reveals the Localisation of a Putatively Identified Sucrose Metabolite Associated to Plasmopara Viticola Development. Frontiers in Plant Science 2022, 13.
5. Maia, M.; Carré, V.; Aziz, A.; Jeandet, P. Molecular Localization of Phytoalexins at the Micron Scale: Toward a Better Understanding of Plant-Phytoalexin-Pathogen Dynamics. J. Agric. Food Chem. 2022.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Vincent Carré1, Maia Marisa1, Aziz Aziz2, Jeandet Philippe2

1. LCP-A2MC, Université de Lorraine, Metz, France
2. RIBP, USC INRAE 1488, Université de Reims Champagne-Ardenne, Reims, France

Contact the author*

Keywords

Mass Spectrometry, Imaging Metabolomics, Plant-Pathogen Interaction, Stilbene phytoalexins

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

IMPACT OF CLIMATIC ZONES ON THE AROMATIC PROFILE OF CORVINA WINES IN THE VALPOLICELLA REGION

In Italy, in the past two decades, the rate of temperature increases (0.0369 °C per year) was slightly higher compared to the world average (0.0313 °C per year). It has also been indicated that the number and intensity of heat waves have increased considerably in the last decades. (IEA, 2022). Viticultural zones can be classified with climatic indexes. Huglin’s index (HI) considers the temperature in a definite area and has been considered as reliable to evaluate the thermal suitability for winegrape production (Zhang et al., 2023).

Read More

IMPACT OF MANNOPROTEIN N-GLYCOSYL PHOSPHORYLATION AND BRANCHING ON WINE POLYPHENOL INTERACTIONS WITH YEAST CELL WALLS

Yeast cell walls (CWs) may adsorb wine components with a significant impact on wine quality. When dealing with red wines, this adsorption is mainly related to physicochemical interactions between wine polyphenols and cell wall mannoproteins. However, mannoproteins are a heterogeneous family of complex peptidoglycans including long and highly branched N-linked oligosaccharides and short linear O-linked oligosaccharides, resulting in a huge structural diversity.

Read More

2-YEARS STUDY ON COMPARISON BETWEEN THE VOLATILE CHEMICAL PROFILE OF TWO DIFFERENT BLENDS FOR THE ENHANCEMENT OF “VALPOLICELLA SUPERIORE”

Valpolicella is a famous wine producing region in the province of Verona owing its fame above all to the production of two Protected Designation of Origins (PDOs) withered wines: Amarone and Recioto. In recent years, however, the wineries have been interested in the enhancement and qualitative increase of another PDO, Valpolicella Superiore. All the Valpolicella PDOs wines are produced with a unique grape blend, mainly Corvina, Corvinone, Rondinella and a range of other minor varieties.From 2019 Valpolicella product regulation has changed the grape proportion of the blend allowing new composition parameters of wines. For this reason, studying the volatile chemical profiles to support wine makers in the effort to produce high quality wines represents a field of great interest.

Read More

EFFECTS OF WINEMAKING FACTORS AND AGEING ON THE POLYPHENOLIC AND COLORIMETRIC PROFILES IN RED WINES PRONE TO COLOUR INSTABILITY

The effects of (A) grape freezing, and (B) malolactic fermentation, have been evaluated on the chemical and colorimetric profiles of red wines from Schiava grossa cv. grapes, thus prone to colour instability. The aim was to observe if specific variables (e.g. grape freezing) could improve the extraction and stability of pigments. The samples were studied from musts up to twelve months in bottle. The study was conducted with independent parallel micro-vinifications (12 = 4 theses x 3 replicates) under strictly-controlled conditions.

Read More

Overhead spray water treatment as a mitigation strategy for reducing vine stress and preserving grape quality during heatwaves

Changes in climate have been influencing the quality of wine grapes worldwide. The impact of extreme climate events over short periods is increasingly recognized as a serious risk to grape quality and yield quantity. In this study the mitigation effects of a pulsed water spray on vine canopy during heatwave events has been evaluated for maintaining vine condition during the growing season and grape quality. Vines of three varieties (Malbec, Bonarda, and Syrah) under drip irrigation in the UNCuyo experimental vineyard were treated with an overhead pulsed water spray.

Read More