terclim by ICS banner
IVES 9 IVES Conference Series 9 PROBING GRAPEVINE-BOTRYTIS CINEREA INTERACTION THROUGH MASS SPECTROMETRY IMAGING

PROBING GRAPEVINE-BOTRYTIS CINEREA INTERACTION THROUGH MASS SPECTROMETRY IMAGING

Abstract

Plants in their natural environment are in continuous interaction with large numbers of potentially pathogenic and beneficial microorganisms. Depending on the microbe, plants have evolved a variety of resistance mechanisms that can be constitutively expressed or induced. Phytoalexins, which are biocidal compounds of low to medium molecular weight synthesized by and accumulated in plants as a response to stress, take part in this intricate defense system.1,2

One of the limitations of our knowledge of phytoalexins is the difficulty of analyzing their spatial responsiveness occurring during plant- pathogen interactions under natural conditions.

Mass spectrometry imaging is an innovative analytical technique for the spatial mapping of molecules within a sample. It has the ability to provide valuable insights into the molecular processes underlying the interaction between a plant and a pathogen. Despite its advantages, to date, the studies of the application of MALDI-MSI to plants is not extensive, even more the ones investigating grapevine compounds.3–5

In a first part, the presentation will focus on the principle and characteristic data provided by this methodology involving a localized ion source and a high-resolution mass spectrometer. The following part will be dedicated to our work on the application of this methodology to investigate phytoalexin accumulation and distribution in leaf tissues of Vitis vinifera cv Chardonnay, infected with Botrytis cinerea, the causal agent for gray mold. We specifically investigate the accumulation and spatial distribution of resveratrol and its derivative oligomers through a time course infection using matrix-assisted laser desorption ionization – mass spectrometry imaging (MALDI-MSI). Our results show that production of stilbene phytoalexins is rapidely activated by the presence of the pathogen, with a high molecular diversity as evidenced by the detection of various oligomeric forms. Moreover, the specific behavior of these compounds towards the propagation of the infection by the pathogen is clearly highlighted at the micron scale.

 

1. Jeandet, P.; Delaunois, B.; Conreux, A.; Donnez, D.; Nuzzo, V.; Cordelier, S.; Clément, C.; Courot, E. Biosynthesis, Metabolism, Molecular Engineering, and Biological Functions of Stilbene Phytoalexins in Plants. BioFactors 2010, 36 (5), 331–341.
2. Jeandet, P.; Uddin, M. S.; Clément, C.; Aziz, A.; Jacquard, C.; Khan, H.; Shah, M. A.; Barka, E. A.; Koffas, M.; Nabavi, S. M.; Sobarzo-Sánchez, E.; Renault, J.-H. Production of High Molecular-Ordered Stilbene Oligomers for the Study of Their Biological Activity: Total Synthesis, Bio-Catalyzed Synthesis and Production by Plant Systems. Nat. Prod. Rep. 2023.
3. Becker, L.; Carré, V.; Poutaraud, A.; Merdinoglu, D.; Chaimbault, P. MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves. Molecules 2014, 19 (7), 10587–10600.
4. Maia, M.; McCann, A.; Malherbe, C.; Far, J.; Cunha, J.; Eiras-Dias, J.; Cordeiro, C.; Eppe, G.; Quinton, L.; Figueiredo, A.; De Pauw, E.; Sousa Silva, M. Grapevine Leaf MALDI-MS Imaging Reveals the Localisation of a Putatively Identified Sucrose Metabolite Associated to Plasmopara Viticola Development. Frontiers in Plant Science 2022, 13.
5. Maia, M.; Carré, V.; Aziz, A.; Jeandet, P. Molecular Localization of Phytoalexins at the Micron Scale: Toward a Better Understanding of Plant-Phytoalexin-Pathogen Dynamics. J. Agric. Food Chem. 2022.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Vincent Carré1, Maia Marisa1, Aziz Aziz2, Jeandet Philippe2

1. LCP-A2MC, Université de Lorraine, Metz, France
2. RIBP, USC INRAE 1488, Université de Reims Champagne-Ardenne, Reims, France

Contact the author*

Keywords

Mass Spectrometry, Imaging Metabolomics, Plant-Pathogen Interaction, Stilbene phytoalexins

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

INVESTIGATION OF FILM COATINGS AS A PROTECTIVE LAYER IN REDUCING THE ABSORPTION OF SMOKE PHENOLS INTO PINOT NOIR GRAPES

Wine grapes exposed to wildfire smoke have resulted in wines with burnt and ashy sensory characteristics¹, that are undesirable qualities in wine. In extreme wildfire events, this can lead to total loss of grape crop. Currently there are no effective solutions in the market to prevent the uptake of smoke compounds into grapes. In this study, previously developed innovative film coatings were tested to analyze their effectiveness in reducing smoke phenol absorption². Four different cellulose nanofiber-based film types were investigated.

UNCOVERING THE ROLE OF BERRY MATURITY STAGE AND GRAPE GENOTYPE ON WINE CHARACTERISTICS: INSIGHTS FROM CHEMICAL CHARACTERISTICS AND VOLATILE COMPOUNDS ANALYSIS

In a climate change context and aiming for sustainable, high-quality Bordeaux wine production, this project examines the impact of grape maturity levels in various cultivars chosen for their adaptability, genetic diversity, and potential to enhance wine quality. The study explores the effects on wine compo-sition and quality through sensory and molecular methods. We studied eight 14-year-old Vitis vinifera cv. grape varieties from the same area (VITADAPT plots 1 and 5): Cabernet Franc, Cabernet Sauvignon, Carmenère, Castets, Cot, Merlot, Petit Verdot, and Touriga Nacional.

IMPACT OF FINING WITH K-CARRAGEENAN, BENTONITE, AND CHITOSAN ON PROTEIN STABILITY AND MACROMOLECULAR COMPOUNDS OF ALBARIÑO WHITE WINE PRODUCED WITH AND WITHOUT PRE-FERMENTATIVE SKIN MACERATION

Pre-fermentative skin maceration is a technique used in white wine production to enhance varietal aroma, but it can increase protein concentration, leading to protein instability and haze formation [1]. To prevent protein instability, wine producers typically use fining agents such as bentonite, before wine bottling, which can negatively impact sensory characteristics and produce waste [2,3]. The aim of this study was to understand the impact of alternative techniques such as the application of polysaccharides (k-carrageenan and chitosan) on protein stability and on the wine macromolecular composition.

Rootstock mediated responses of grapevine (Vitis vinifera L.) metabolism and physiology to combined water deficit and salinity stress in Syrah grafts

Water deficit and salinity are increasingly affecting the viticulture and wine industry. These two stresses are intimately related; understanding the physiological and metabolic responses of grapevines to water deficit, salinity and combined stress is critical for developing strategies to mitigate the nega- tive impacts of these stresses on wine grape production. These strategies can include selecting more tolerant grapevine cultivars and graft combinations, improving irrigation management, and using soil amendments to reduce the effects of salinity. For this purpose, understanding the response of grape- vine metabolism to altered water balance and salinity is of pivotal importance.

AROMA AND SENSORY CHARACTERIZATION OF XINOMAVRO RED WINES FROM DIFFERENT GREEK PROTECTED DESIGNATIONS OF ORIGIN, EFFECT OF TERROIR CHARACTERISTICS

The quality of wines has often been associated with their geographical area of production. The aim of this work was to characterize Protected Designation of Origin (PDO) Xinomavro red wines from different geographical areas of Amyndeon and Naoussa in Northern Greece, elaborated with variables that contribute to their differentiation, such as soil characteristics, altitude, monthly average temperature and rainfall.
Xinomavro fruit parcels from different vineyards within the two PDO zones (5 PDO Naoussa and 6 PDO Amyndeon) were vinified following a standard winemaking process. A total of 25 aroma compounds were quantified using gas chromatography-mass spectrometry (GC-MS) with simultaneous full scan and selected ion monitoring for data recording, and odor activity values (OAVs) were determined.