terclim by ICS banner
IVES 9 IVES Conference Series 9 METABOLIC INTERACTIONS OF SACCHAROMYCES CEREVISIAE COCULTURES: A WAY TO EXTEND THE AROMA DIVERSITY OF CHARDONNAY WINE

METABOLIC INTERACTIONS OF SACCHAROMYCES CEREVISIAE COCULTURES: A WAY TO EXTEND THE AROMA DIVERSITY OF CHARDONNAY WINE

Abstract

Yeast co-inoculations in winemaking have been investigated in various applications, but most often in the context of modulating the aromatic profiles of wines. Our study aimed to characterize S. cerevisiae interactions and their impact on wine by taking an integrative approach. Three cocultures and corresponding pure cultures of S. cerevisiae were characterized according to their fermentative capacities, the chemical composition and aromatic profile of the associated Chardonnay wines. The various strains studied within the cocultures showed different behaviors regarding their development. More than half of the 67 volatile compounds quantified were modulated by interactions, including 18 relevant wine aroma compounds. The main families affected were higher alcohols and their associated esters, vinyl phenols, and fatty acids. Coculture makes it possible to obtain new aromatic expressions that do not exist in the original pure cultures attributed to yeast interactions. The sensory profile of the wines related to the cocultures differed from the wines associated with the pure cultures. However, they also differed from the blends (50/50 v/v) of post AF wines from pure cultures. Based on the exometabolome, this was confirmed. The cocultures were revealed as not being simple additions of two wines represented by blend, thereby indicating complex interactions. High resolution mass spectrometry allowed to highlight thousands of cocultures biomarkers. Most of these biomarkers belonged to metabolic pathways involved in nitrogen metabolism. The latter is therefore a marker of changes associated with interactions between two strains of S. cerevisiae. Despite of preserved fermentative properties, the described interactions in- duced a modification of the chemical composition and sensory profile of the wines from the cocultures. A comprehensive approach by combining different techniques is essential to understand yeast interactions and describe the consequences on wine.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Fanny Bordet 1,4, Rémy Romanet1, Florian Bahut1,4, Jordi Ballester2, Camille Eicher1, Cristina Peña3, Vicente Ferreira3, Régis Gou-geon1,5, Anne Julien-Ortiz4, Chloé Roullier-Gall1, Hervé Alexandre1

1. Univ. Bourgogne Franche-Comté, Institut Agro Dijon, PAM UMR A 02.102, 21000 Dijon, France, IUVV, Rue Claude Ladrey, 21000 Dijon, France
2. Centre des Sciences du Goût et de l’Alimentation, Institut Agro Dijon, CNRS, INRA, Université Bourgogne – Franche-Comté, 21000 Dijon, France
3. University of Zaragoza, Dpt. Química Analítica. Facultad de Ciencias, 50009 Zaragoza, Spain
4. Lallemand SAS, 19 rue des Briquetiers, 31000 Blagnac, France
5. DIVVA (Développement Innovation Vigne Vin Aliments) Platform/PAM UMR, IUVV, Rue Claude Ladrey, 21000 Dijon, France

Contact the author*

Keywords

fermentation, interactions, Saccharomyces cervevisiae, metabolomic

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

ASSESSMENT OF ‘DOLCETTO’ GRAPES AND WINES FROM DIFFERENT AREAS OF OVADA DOCG

Dolcetto (Vitis vinifera L.) is one of the traditionally cultivated varieties in Piedmont (north-east Italy). Dolcetto wines have long been associated with local consumption and they are little known internationally. In particular, the Ovada area (south-east Piedmont), even if it represents a small share of the regional PDO Dolcetto production, is one of the oldest and vocated territory, giving wine also suitable for aging. In this study, the basic composition and phenolic content of Dolcetto grapes for Ovada DOCG wines have been investigated in three different vintages (2020-2022), as well as the main aspects of the derived commercial and experimental wines (basic parameters, phenolics, volatile compounds, sensory properties).

EFFECTS OF INDUCED SUNBURN DAMAGES ON WHITE WINE PROPERTIES

Climate change is a great challenge for the environment and affects the wine industry as well. Sunburn damage of sensitive grapes increase with severe heat periods. Besides significant loss of yield sunburn, modifies sensory properties of the wines and may cause climate-related off-flavours. To initiate sunburn in a controlled way, in 2021 sunburn was directly induced in the vineyard with the GrapeBurner device, exposing grapes of the varieties Riesling and Pinot Blanc with UV and IR radiation. This device was first assembled by Kai Müller of the university in Geisenheim and consists of a carriage with 6 UV/IR lamps. A 15 min irradiation was applied in early September at 60°Oe. Due to the colder season in 2021 the grapes were not harmed by previous sunburn damage.

INFLUENCE OF THE NITROGEN / LIPIDS RATIO OF MUSTS ON THE REVELATION OF AROMATIC COMPOUNDS IN SAUVIGNON BLANC WINE

Production of volatile compounds by yeast is known to be modulated by must nitrogen. Nevertheless, various parameter of must quality have an impact on yeast fermentation. In this study we propose to evaluate the impact of nitrogen / lipids balance on a Sauvignon Blanc grape juice (Val de Loire).
Must was prepared from the same grapes at pilot scale. Three modalities were carried out: direct pressing, direct pressing with a pre-fermentation cold stabulation and pellicular maceration before pressing.

SENSORY DEFINITION OF A TECHNICAL UNAVOIDABLE TRANSFER OF AROMA COMPOUNDS VIA SEALING IN A BOTTLING LINE IN ORDER TO PREVENT PROSECUTION DUE TO FRAUDULENT AROMATIZATION OF A SUBSEQUENTLY FILLED WINE

In 2020, 12% of all bottled German wines were aromatized, which may increase further due to rising popularity of dealcoholized wines. As sealing polymers of a bottling line absorb aroma compounds and may release them into regular wines in the next filling¹, this unintentional carry-over bears the risk to violate the legal ban of any aromatization of regular wine. However, following EU legislation, German food control authorities accept a technical unavoidable transfer of aroma compounds, if this is of no sensory significance.

A NEW STRATEGY AND METHODOLOGY FOR THE CHARACTERIZATION OF POLYPHENOLS IN FINING PRECIPITATE

Polyphenols are secondary metabolite widely distributed in plant kingdom such as in fruits, in grapes and in wine. During the winemaking process, polyphenols are extract from the skin and seed of the berries. Fining is an important winemaking step just before bottling which has an impact on wine stabilization and clarification. Most the time, fining agent are animal or vegetal protein while some of them can be synthetic polymer like PVPP or natural origin like bentonite.