terclim by ICS banner
IVES 9 IVES Conference Series 9 ASSESSING THE ROLE OF 27 KNOWN BITTER COMPOUNDS IN COMMERCIAL WHITE WINES COMBINING LC-MS QUANTIFICATION AND SENSORY ANALYSIS

ASSESSING THE ROLE OF 27 KNOWN BITTER COMPOUNDS IN COMMERCIAL WHITE WINES COMBINING LC-MS QUANTIFICATION AND SENSORY ANALYSIS

Abstract

The balance between the different flavours of a wine largely determines its perception and appreciation by the consumers. In white wines, sweetness and sourness are usually the two poles balancing the taste properties. The bitter flavour, on the other hand, is frequently associated with a loss of equilibrium and all white wines (dry and sweet, young and aged) are affected.

Several bitter compounds are already well-described in wines. Some are linked to microorganisms as acrolein (Bauer et al., 2010) or oak wood, for example lyoniresinol (Cretin et al., 2015), while others come directly from grapes: mostly phenolic (Hufnagel and Hofmann, 2008) and nitrogen compounds (Roudot-Algaron, 1996). Furthermore, the enhancing role played by ethanol has also been well established (Cretin et al., 2018). The present study aims to determine the influence of twenty-seven known bitter compounds on the taste of various commercial white wines.

Thirty wines have been selected and submitted to sensory analysis by a trained panel. The various intensities of sourness, sweetness and bitterness have been determined for each wine. Jointly, five quantification methods have been developed and validated using liquid chromatography coupled with high resolution mass spectrometry (UHPLC-Exactive, Orbitrap analyzer) in order to determine the amount of the selected bitter compounds.

Potential correlations between the described tastes of the wines and concentrations of bitter molecules have been assessed. For the most relevant compounds, detection thresholds have been updated using the same trained panel, enabling a better understanding of the impact of various compounds.

This study enlightens the role of already known bitter compounds in bitter wine. It is also leading the way to further research as some wine’s taste remain unexplained by the selected compounds, thus confirming the potential presence of still unknown bitter compounds.

 

1. Bauer, R., Cowan, D. A., Crouch, A., 2010. Acrolein in wine : importance of 3-hydroxypropionaldehyde and derivatives in production and detection. J. Agric. Food Chem. 58, 3243-3250.
2. Cretin, B., Sallembien, Q., Sindt, L., Daugey, N., Buffeteau, T., Waffo-Teguo, P., Dubourdieu, D., Marchal, A., 2015. How stereochemistry influences the taste of wine : Isolation, characterization and sensory evaluation of lyoniresinol stereoisomers. Analytica chimica acta. 888, 191-198.
3. Cretin, B., Dubourdieu, D., Marchal, A., 2018. Influence of ethanol content on sweetness and bitterness perception in dry wines. Food science & technology. 87, 61-66.
4. Hufnagel, J.C., Hofmann, T., 2008. Quantitative reconstruction of the nonvolatile sensometabolome of a red wine. J. Agric. Food Chem. 56, 9190-9199.
5. Roudot-Algaron, F., 1996. Le goût des acides aminés, des peptides et des protéines : exemple de peptides sapides dans les hydrolysats de caséines. Lait. 76, 313-348.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Tom Estier1,2 and Axel Marchal1,2

1. Univ. Bordeaux, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
2. Bordeaux Sciences Agro, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, F-33170 Gradignan, France

Contact the author*

Keywords

LC-MS quantification, sensory analysis, bitterness, wine

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

CHARACTERIZATION OF THE VOLATILE COMPOUNDS PROFILE OF COMMERCIAL GRAPPAS OBTAINED FROM THE POMACE OF AMARONE WINES

Grappa is a traditional Italian alcoholic beverage, with an alcohol content generally between 40-60% vol., obtained from the distillation of grape pomace used for the production of wine. Grappa are often aged in wooden barrels. There are various types of grappa: young, aromatic, aged, extra-aged depending on whether the distillate comes from aromatic vines or is aged in wooden barrels for shorter or longer periods. There is also flavored grappa if herbs, fruit or roots are added. All this makes it an extremely heterogeneous product both from an organoleptic and compositional point of view.

SUB-CRITICAL WATER: AN ORIGINAL PROCESS TO EXTRACT ANTIOXIDANTS COMPOUNDS OF WINE LEES

Wine lees are quantitatively the second most important wine by-product after grape stems and marc [1]. In order to recycle, distilleries recovered ethanol and tartaric acid contained in wine lees but yeast biomass is often unused. It has already been demonstrated that this yeast biomass could be upcycled to produce yeast extracts of interest for wine chemical stabilization [2]. In addition, it is well known that lees, during aging, release compounds that preserve wine from oxidation.

EXPLORING THE METABOLIC AND PHENOTYPIC DIVERSITY OF INDIGENOUS YEASTS ISOLATED FROM GREEK WINE

Climate change leads to even more hostile and stressful for the wine microorganism conditions and consequently issues with fermentation rate progression and off-character formation are frequently observed. The objective of the current research was to classify a great collection of yeast isolates from Greek wines based on their technological properties with oenological interest. Towards this direction, fourteen spontaneously fermented wines from different regions of Greece were collected for further yeast typing. The yeast isolates were subjected in molecular analyses and identification at species level.

IMPACT OF CLIMATIC ZONES ON THE AROMATIC PROFILE OF CORVINA WINES IN THE VALPOLICELLA REGION

In Italy, in the past two decades, the rate of temperature increases (0.0369 °C per year) was slightly higher compared to the world average (0.0313 °C per year). It has also been indicated that the number and intensity of heat waves have increased considerably in the last decades. (IEA, 2022). Viticultural zones can be classified with climatic indexes. Huglin’s index (HI) considers the temperature in a definite area and has been considered as reliable to evaluate the thermal suitability for winegrape production (Zhang et al., 2023).

YEAST DERIVATIVE PRODUCTS: CHARACTERIZATION AND IMPACT ON RIBOFLAVIN RELEASE DURING THE ALCOHOLIC FERMENTATION

Light-struck taste (LST) is a wine fault that can occur in white and sparkling wines when exposed to light. This defect is mainly associated to the formation of methanethiol and dimethyl disulfide due to light-induced reactions involving riboflavin (RF) and methionine [1]. The presence of RF in wine is mainly due to the metabolism of yeast [2] which fermenting activity can be favoured by using yeast derivative products (YDPs) as nutrients. Nonetheless, a previous study showed the addition of YDPs before the alcoholic fermentation (AF) led to higher concentrations of RF in wines [3]. Due to the widespread use of YDPs in the winemaking process, this study aimed to understand the possible relation between the content of RF in wine and the YDP adopted as nutrient for AF.